Genes and uses thereof to modulate secondary metabolite biosynthesis

ABSTRACT

The present invention relates to the use of a genome wide expression profiling technology in combination with the detection of the presence of secondary metabolites of interest to isolate genes that can be used to modulate the production of secondary metabolites in organisms and cell lines derived therefrom.

CROSS-REFERENCE TO RELATED APPLICATION

This application is a continuation of PCT International PatentApplication No. PCT/EP04/50171, filed on May 16, 2003, designating theUnited States of America, and published, in English, as PCTInternational Publication No. WO 03/097790 A2 on Nov. 27, 2003, thecontents of the entirety of which is incorporated by this reference.

SEQUENCE LISTING

Submitted with this application is a compact disc containing a SEQUENCELISTING in a file entitled “V116.5T25 seq list” (524 KB, file createdNov. 17, 2004), the material contained in the compact disc beingincorporated herein by this reference in its entirety. There are twoidentical compact discs submitted with this patent application (i.e.,“Copy 1” and “Copy 2”), one being a copy of the other and eachcontaining the single file “V116.5T25 seq list” (524 KB, file createdNov. 17, 2004).

TECHNICAL FIELD

The present invention relates generally to biotechnology, and, moreparticularly, to the use of a genome wide expression profilingtechnology in combination with the detection of the presence ofsecondary metabolites of interest to isolate genes that can be used tomodulate the production of secondary metabolites in organisms and celllines derived thereof.

BACKGROUND

Terrestrial micro-organisms, fungi, invertebrates and plants havehistorically been used as sources of natural products. However, apartfrom several well-studied groups or organisms, such as theactinomycetes, which have been developed for drug screening andcommercial production, production problems still exist. For example, theantitumor agent taxol is a constituent of the bark of mature Pacific yewtrees and its usage as a drug agent has caused concern about cutting toomany of these trees and causing damage to the local ecological system.Taxol contains 11 chiral centers with 2048 possible diastereoisomericforms so that its de novo synthesis on a commercial scale is unlikely.Furthermore, certain compounds appear in nature only when specificorganisms interact with each other and the environment. Pathogens mayalter plant gene expression and trigger synthesis of secondarymetabolites such as phytoalexins that enable the plant to resist attack.Moreover, a lead compound discovered through random screening rarelybecomes a drug because its bioavailability may not be adequate.Typically, a certain quantity of the lead compound is required so thatit can be modified structurally to improve its initial activity.However, current methods for synthesis and development of lead compoundsfrom natural sources, especially plants, are relatively inefficient.Other valuable phytochemicals are quite expensive because they are onlyproduced at extremely low levels. These problems also delay clinicaltesting of new compounds and affect the economics of using these newsources of drug leads. The problems of obtaining useful metabolites fromnatural sources in high quantities may potentially be circumvented bycell cultures. For example the culture of plant cells has been exploredsince the 1960' as a viable alternative for the production of complexphytochemicals of industrial interest. However, despite promisingfeatures and developments, the production of plant-derivedpharmaceuticals by plant cell cultures has not been fully commerciallyexploited. The main reasons for this reluctance are economical onesbased on the slow growth and the low production levels of secondarymetabolites by such plant cell cultures. However, little is known abouthow plants synthesize secondary metabolites and very little is knownabout how this synthesis is regulated. Certainly there is a need for amethod to obtain higher levels of valuable secondary metabolite. Thelatter may include the identification of biosynthetic genes andregulatory genes involved in secondary metabolite biosynthetic pathways.Although genome sequencing of many organisms is now advancing at afrenetic pace, the metabolic pathways of most of the natural productsare not understood. Traditional textbook representations of metabolicpathways neither capture the full number of potential network functionsnor the network's resilience to disruption. Whereas algorithmicapproaches to these latter problems have been proposed, many aspects ofmetabolic network function remain to be clearly delineated. Numerousstudies have investigated the enzymes and regulatory factors controllingbiosynthesis of specific secondary metabolites but little is known aboutthe genetics controlling the quantitative and qualitative naturalvariation in secondary chemistry (QTL-approach, Kliebenstein et al.(2001) Genetics 159: 359, isolation of expressed sequence tags, Sheltonet al. (2002) Plant Science 162, 9, Lange et al. (2000) Proc. Natl.Acad. Sci. 97, 2934, a proteomics approach, Decker et al. (2000)Electrophoresis 21, 3500).

DISCLOSURE OF THE INVENTION

In the present invention, we provide a method that follows a genome wideapproach and correlates gene expression with the production of secondarymetabolites. Thus, through the combination of metabolic profiling andcDNA-AFLP based transcript profiling of elicited tobacco cells we haveisolated genes that are involved in the production of alkaloids andphenylpropanoids. These genes can be used to modulate the production ofsecondary metabolites in plant cells.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1: Semi-hypothetic scheme of the biosynthesis of nicotine alkaloidsin Nicotiana tabacum leaves and BY-2 cells

FIG. 2: The growth curve of tobacco BY-2 cells, determined by packedcell volume (PVC)

FIG. 3: Molecular formulas of the tobacco alkaloids detected from BY-2cells after elicitation with methyl jasmonate

FIG. 4: Nicotine and anabasine content [ug/g (d.w.)] after elicitationwith 50 μM MeJA. Each sample was pooled together from three replicateshake flasks

FIG. 5: Anatabine and anatalline contents [ug/g (d.w.)] afterelicitation with 50 μM MeJA. Each sample was pooled together from threereplicate shake flasks

FIG. 6: Time-course of the accumulation of alkaloids in elicited BY-2cells. Logarithmic scale

FIG. 7: The content of methyl putrecine in free pool of tobacco BY-2cells.

FIG. 8: The content of polyamines (mean, SD, n=3) in free pool oftobacco BY-2 cells

FIG. 9: The content of soluble conjugated polyamines (mean, SD, n=3) intobacco BY-2 cells

FIG. 10: The content of insoluble conjugated polyamines (mean, SD, n=3)in tobacco BY-2 cells

FIG. 11: Functional analysis. Nicotine content in elicitated (50 μMMeJA) BY-2 cells (N=3)

FIG. 12: Functional analysis. Anabasine content in elicitated (50 μMMeJA) BY-2 cells (N=3)

FIG. 13: Functional analysis. Anatabine content in elicitated (50 μMMeJA) BY-2 cells (N=3)

FIG. 14: Functional analysis. Anatalline (1 & 2) content in elicitated(50 μM MeJA) BY-2 cells (N=3)

DETAILED DESCRIPTION OF THE INVENTION

There has always been interest in natural products for flavourings forfood, perfumes, pigments for artwork and clothing, and tools to achievespiritual enlightenment. Especially plant derived drugs are among theoldest drugs in medicine. For example alkaloids are originally describedas structually diverse class of plant derived nitrogenous compounds,which often possess strong physiological activity. Plants synthesizealkaloids for various defence-related reactions, for example, actionsagainst pathogens or herbivores. Over 15.000 alkaloids have beenidentified from plants. Alkaloids are classified into severalbiogenically related groups, but the enzymes and genes have been partlycharacterised only in groups of nicotine and tropane alkaloids, indolealkaloids and isoquinolidine alkaloids (Suzuki et al., 1999). Nicotineand tropane alkaloids share partly the same biosynthetic pathway. Manyplants belonging to, for example, the Solanaceae family have been usedfor centuries because of their active substances: hyoscyamine andscopolamine. Also other Solanaceae plants belonging to the generaAtropa, Datura, Duboisia and Scopolia produce these valuable alkaloids.In medicine they find important applications in ophthalmology,anaesthesia, and in the treatment of cardiac and gastrointestinaldiseases. Although a lot of information is available on thepharmacological effects of tropane alkaloids, surprisingly little isknown about how plants synthesize these substances and almost nothing isknown about how this synthesis is regulated. Nicotine is found in thegenus Nicotiana and also other genera of Solanaceae and is also presentin many other plants including lycopods and horsetails (Flores et al.,1991). Saitoh et al. (1985) performed an extensive study of the nicotinecontent in 52 of the 66 Nicotiana species and concluded that eithernicotine or nornicotine is the predominant alkaloid in the leaves,depending on the species. However, in roots nicotine dominates in almostall species. In callus cultures, the nicotine content is mostlyremarkably lower than in intact plants. The highest production has beenfound in the BY-2 cell line: 2.14% on dry weight basis which resemblesthe nicotine content in intact tobacco plants (Ohta et al., 1978).Although much is known of the alkaloid metabolite content in differentorgans of tobacco, surprisingly little is known about the biosynthesis,metabolism and regulation of various nicotine alkaloids in tobaccocallus and cell cultures.

Many approaches have been developed to overcome the common problem oflow product yield of alkaloid-producing plant cell cultures. Oneapproach is the addition of elicitors. Elicitors are compounds capableof inducing defence responses in plants (Darvil and Albersheim, 1984).Other approaches to increase the product yield of secondary metabolitescomprise the screening and selection of high-producing cell lines, theoptimisation of the growth and product parameters and the use ofmetabolic engineering (Verpoorte et al., 2000). However, metabolicengineering implies detailed knowledge of the biosynthetic steps of thesecondary metabolite(s) of interest. Progress in the elucidation of thebiosynthetic pathways of plant secondary products has long been hamperedby lack of good model systems. In the past two decades plant cellcultures have proven to be invaluable tools in the investigation ofplant secondary metabolite biosynthetic pathways. The tobacco BY-2(Nicotiana tabacum var. “Bright Yellow”) cell line is a very fastgrowing and highly synchronisable cell system and thus desirable forinvestigation of various aspects of plant cell biology and metabolism(Nagata and Kumagai, 1999). In the present invention the formation ofvarious nicotine related alkaloids in tobacco BY-2 cells was taken as anexample for the isolation of genes involved in the biosynthesis ofalkaloids, phenylpropanoids and other secondary metabolites. We haveused a genome wide approach and isolated genes which expressioncorrelated with the occurrence of alkaloids and/or phenylpropanoids.

In one embodiment, the invention provides an isolated polypeptidemodulating the production of at least one secondary metabolite in anorganism or cell derived thereof selected from the group consisting of(a) polypeptide encoded by a polynucleotide comprising SEQ ID NO: 1, 2,3, through 609, 610, 611 or SEQ ID NO: 612, 613, 614, through 869, 870,871 of the accompanying and incorporated herein by reference SEQUENCELISTING, (b) a polypeptide comprising a polypeptide sequence having aleast 60% identity to at least one of the polypeptides encoded by apolynucleotide sequence having SEQ ID NO: 612, 613, 614 through 869,870, 871, (c) a polypeptide comprising a polypeptide sequence having aleast 90% identity to at least one of the polypeptides encoded by apolynucleotide sequence having SEQ ID NO: 1, 2, 3 through 609, 610, 611and (d) fragments and variants of the polypeptides according to (a), (b)or (c) modulating the production of at least one secondary metabolite inan organism or cell derived thereof.

In another embodiment, the invention provides an isolated polypeptideaccording to wherein the polypeptide sequence is depicted in SEQ ID NO:872, 873, 874 through 894 or 895 and polypeptide sequences having atleast 90% identity to SEQ ID NO: 872, 873, 874 through 894 or 895.

In another embodiment, the invention provides an isolated polynucleotideselected from the groups consisting of (a) polynucleotide comprising apolynucleotide sequence having at least one of the sequences SEQ ID NO:1, 2, 3 through 609, 610, 611 or SEQ ID NO: 612, 613, 614 through 869,870, 871; (b) a polynucleotide comprising a polynucleotide sequencehaving at least 60% identity to at least one of the sequences having SEQID NO: 612, 613, 614, . . . , 869, 870, 871; (c) a polynucleotidecomprising a polynucleotide sequence having at least 90% identity to atleast one of the sequences having SEQ ID NO: 1, 2, 3 through 609, 610,611; (d) fragments and variants of the polynucleotides according to (a),(b) or (c) modulating the production of at least one secondarymetabolite in an organism or cell derived thereof.

Accordingly, the present invention provides 611 polynucleotide sequences(SEQ ID NO: 1, 2, 3 through 609, 610, 611) derived from tobaccoBY2-cells for which a homologue exists in other species and 260polynucleotide sequences (SEQ ID NO: 612, 613, 614 through 869, 870,871) derived from tobacco BY2-cells for which no homologue exists inother species. As used herein, the word “polynucleotide” may beinterpreted to mean the DNA and cDNA sequence as detailed by Yoshikai etal. (1990) Gene 87:257, with or without a promoter DNA sequence asdescribed by Salbaum et al. (1988) EMBO J. 7(9):2807.

As used herein, “fragment” refers to a polypeptide or polynucleotide ofat least about 9 amino acids or 27 base pairs, typically 50 to 75, ormore amino acids or base pairs, wherein the polypeptide contains anamino acid core sequence. If desired, the fragment may be fused ateither terminus to additional amino acids or base pairs, which maynumber from 1 to 20, typically 50 to 100, but up to 250 to 500 or more.A “functional fragment” means a polypeptide fragment possessing thebiological property able to modulate the production of at least onesecondary metabolite in an organism or cell derived thereof. In aparticular embodiment the functional fragment is able to modulate theproduction of at least one secondary metabolite in a plant or plant cellderived thereof. The term ‘production’ includes intracellular productionand secretion into the medium. The term ‘modulates or modulation’ refersto an increase or a decrease. Often an increase of at least onesecondary metabolite is desired but sometimes a decrease of at least onesecondary metabolite is wanted. The decrease can for example refer tothe decrease of an undesired intermediate product of at least onesecondary metabolite. With an increase in the production of one or moremetabolites it is understood that the production may be enhanced by atleast 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or at least 100% relativeto the untransformed plant or plant cell which was used to transformwith an expression vector comprising an expression cassette furthercomprising at least one polynucleotide or homologue or variant orfragment thereof of the invention. Conversely, a decrease in theproduction of the level of one or more secondary metabolites may bedecreased by at least 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or at least100% relative to the untransformed plant or plant cell which was used totransform with an expression vector comprising an expression cassettefurther comprising at least one polynucleotide or homologue or variantor fragment thereof of the invention. The terms ‘identical’ or percent‘identity’ in the context of two or more nucleic acids or polypeptidesequences, refer to two or more sequences or subsequences that are thesame or have a specified percentage of amino acid residues ornucleotides that are the same (i.e. 70% identity over a specifiedregion), when compared and aligned for maximum correspondence over acomparison window, or designated region as measured using sequencecomparison algorithms or by manual alignment and visual inspection.Preferably, the identity exists over a region that is at least about 25amino acids or nucleotides in length, or more preferably over a regionthat is 50-100 amino acids or nucleotides or even more in length.Examples of useful algorithms are PILEUP (Higgins & Sharp, CABIOS 5:151(1989), BLAST and BLAST 2.0 (Altschul et al., J. Mol. Biol. 215: 403(1990). Software for performing BLAST analyses is publicly availablethrough the National Center for Biotechnology Informationwww.ncbi.nlm.nih.gov. In the present invention the term ‘homologue’ alsorefers to ‘identity’. For example a homologue of SEQ ID NO: 1, 2, 3through 609, 610 or 611 has at least 90% identity to one of thesesequences. A homologue of SEQ ID NO: 612, 613, 614 through 869, 870 or871 has at least 60% identity to one of these sequences.

According to still further features in the described preferredembodiments, the polynucleotide fragment encodes a polypeptide able tomodulate the secondary metabolite biosynthesis, which may therefore beallelic, species and/or induced variant of the amino acid sequence setforth in SEQ ID NO: 1-871. It is understood that any such variant mayalso be considered a homologue.

The present invention accordingly provides, in one embodiment, a methodfor modulating the production of at least one secondary metabolite inbiological cells or organisms, such as plants, by transformation of thebiological cells with an expression vector comprising an expressioncassette that further comprises at least one gene comprising a fragment,variant or homologue encoded by at least one sequence selected from SEQID NO: 1-871. With “at least one secondary metabolite” it is meant oneparticular secondary metabolite such as for example nicotine or severalalkaloids related with nicotine or several unrelated secondarymetabolites. Biological cells can be plant cells, fungal cells, bacteriacells, algae cells and/or animal cells. In a particular preferredembodiment the biological cells are plant cells. Generally, two basictypes of metabolites are synthesised in cells, i.e. those referred to asprimary metabolites and those referred to as secondary metabolites. Aprimary metabolite is any intermediate in, or product of the primarymetabolism in cells. The primary metabolism in cells is the sum ofmetabolic activities that are common to most, if not all, living cellsand are necessary for basal growth and maintenance of the cells. Primarymetabolism thus includes pathways for generally modifying andsynthesising certain carbohydrates, amino acids, fats and nucleic acids,with the compounds involved in the pathways being designated primarymetabolites. In contrast hereto, secondary metabolites usually do notappear to participate directly in growth and development. They are agroup of chemically very diverse products that often have a restrictedtaxonomic distribution. Secondary metabolites normally exist as membersof closely related chemical families, usually of a molecular weight ofless than 1500 Dalton, although some bacterial toxins are considerablylonger. Secondary plant metabolites include e.g., alkaloid compounds(e.g., terpenoid indole alkaloids, tropane alkaloids, steroidalkaloids), phenolic compounds (e.g., quinines, lignans and flavonoids),terpenoid compounds (e.g., monoterpenoids, iridoids, sesquiterpenoids,diterpenoids and triterpenoids). In addition, secondary metabolitesinclude small molecules, such as substituted heterocyclic compoundswhich may be monocyclic or polycyclic, fused or bridged. Many plantsecondary metabolites have value as pharmaceuticals. Examples of plantpharmaceuticals include, for example, taxol, digoxin, scopolamine,diosgenin, codeine, morphine, quinine, shikonin, ajmalicine andvinblastine.

In another embodiment, the invention provides a recombinant DNA vectorcomprising at least one polynucleotide sequence, homologue, fragment orvariant selected from at least one of the sequences comprising SEQ IDNO: 1-871. The vector may be of any suitable type including, but notlimited to, a phage, virus, plasmid, phagemid, cosmid, bacmid or even anartificial chromosome. The at least one polynucleotide sequencepreferably codes for at least one polypeptide that is involved in thebiosynthesis and/or regulation of synthesis of at least one secondarymetabolite (e.g., a transcription factor, a repressor, an enzyme thatregulates a feed-back loop, a transporter, a chaperone). The term“recombinant DNA vector” as used herein refers to DNA sequencescontaining a desired coding sequence and appropriate DNA sequencesnecessary for the expression of the operably linked codingpolynucleotide sequence in a particular host organism (e.g., plantcell). Plant cells are known to utilize promoters, polyadenlyationsignals and enhancers.

In yet another embodiment, the invention provides a transgenic plant orderived cell thereof transformed with the recombinant DNA vector.

A recombinant DNA vector comprises at least one “Expression cassette”.Expression cassettes are generally DNA constructs preferably including(5′ to 3′ in the direction of transcription): a promoter region, apolynucleotide sequence, homologue, variant or fragment thereof of thepresent invention operatively linked with the transcription initiationregion, and a termination sequence including a stop signal for RNApolymerase and a polyadenylation signal. It is understood that all ofthese regions should be capable of operating in biological cells, suchas plant cells, to be transformed. The promoter region comprising thetranscription initiation region, which preferably includes the RNApolymerase binding site, and the polyadenylation signal may be native tothe biological cell to be transformed or may be derived from analternative source, where the region is functional in the biologicalcell.

The polynucleotide sequence, homologue, variant or fragment thereof ofthe invention may be expressed in for example a plant cell under thecontrol of a promoter that directs constitutive expression or regulatedexpression. Regulated expression comprises temporally or spatiallyregulated expression and any other form of inducible or repressibleexpression. Temporally means that the expression is induced at a certaintime point, for instance, when a certain growth rate of the plant cellculture is obtained (e.g., the promoter is induced only in thestationary phase or at a certain stage of development). “Spatially”means that the promoter is only active in specific organs, tissues, orcells (e.g., only in roots, leaves, epidermis, guard cells or the like).Other examples of regulated expression comprise promoters whose activityis induced or repressed by adding chemical or physical stimuli to theplant cell. In a preferred embodiment the expression is under control ofenvironmental, hormonal, chemical, and/or developmental signals. Suchpromoters for plant cells include promoters that are regulated by (1)heat, (2) light, (3) hormones, such as abscisic acid and methyljasmonate (4) wounding or (5) chemicals such as salicylic acid,chitosans or metals. Indeed, it is well known that the expression ofsecondary metabolites can be boosted by the addition of for examplespecific chemicals, jasmonate and elicitors. In a particular embodimentthe co-expression of several (more than one) polynucleotide sequence orhomologue or variant or fragment thereof, in combination with theinduction of secondary metabolite synthesis is beneficial for an optimaland enhanced production of secondary metabolites. Alternatively, the atleast one polynucleotide sequence, homologue, variant or fragmentthereof is placed under the control of a constitutive promoter. Aconstitutive promoter directs expression in a wide range of cells undera wide range of conditions. Examples of constitutive plant promotersuseful for expressing heterologous polypeptides in plant cells include,but are not limited to, the cauliflower mosaic virus (CaMV) 35Spromoter, which confers constitutive, high-level expression in mostplant tissues including monocots; the nopaline synthase promoter and theoctopine synthase promoter. The expression cassette is usually providedin a DNA or RNA construct which is typically called an “expressionvector” which is any genetic element, for example, a plasmid, achromosome, a virus, behaving either as an autonomous unit ofpolynucleotide replication within a cell (i.e. capable of replicationunder its own control) or being rendered capable of replication byinsertion into a host cell chromosome, having attached to it anotherpolynucleotide segment, so as to bring about the replication and/orexpression of the attached segment. Suitable vectors include, but arenot limited to, plasmids, bacteriophages, cosmids, plant viruses andartificial chromosomes. The expression cassette may be provided in a DNAconstruct which also has at least one replication system. In addition tothe replication system, there will frequently be at least one markerpresent, which may be useful in one or more hosts, or different markersfor individual hosts. The markers may a) code for protection against abiocide, such as antibiotics, toxins, heavy metals, certain sugars orthe like; b) provide complementation, by imparting prototrophy to anauxotrophic host: or c) provide a visible phenotype through theproduction of a novel compound in the plant. Exemplary genes which maybe employed include neomycin phosphotransferase (NPTII), hygromycinphosphotransferase (HPT), chloramphenicol acetyltransferase (CAT),nitrilase, and the gentamicin resistance gene. For plant host selection,non-limiting examples of suitable markers are β-glucuronidase, providingindigo production, luciferase, providing visible light production, GreenFluorescent Protein and variants thereof, NPTII, providing kanamycinresistance or G418 resistance, HPT, providing hygromycin resistance, andthe mutated aroA gene, providing glyphosate resistance.

The term “promoter activity” refers to the extent of transcription of apolynucleotide sequence, homologue, variant or fragment thereof that isoperably linked to the promoter whose promoter activity is beingmeasured. The promoter activity may be measured directly by measuringthe amount of RNA transcript produced, for example by Northern blot orindirectly by measuring the product coded for by the RNA transcript,such as when a reporter gene is linked to the promoter. The term“operably linked” refers to linkage of a DNA segment to another DNAsegment in such a way as to allow the segments to function in theirintended manners. A DNA sequence encoding a gene product is operablylinked to a regulatory sequence when it is ligated to the regulatorysequence, such as, for example a promoter, in a manner which allowsmodulation of transcription of the DNA sequence, directly or indirectly.For example, a DNA sequence is operably linked to a promoter when it isligated to the promoter downstream with respect to the transcriptioninitiation site of the promoter and allows transcription elongation toproceed through the DNA sequence. A DNA for a signal sequence isoperably linked to DNA coding for a polypeptide if it is expressed as apre-protein that participates in the transport of the polypeptide.Linkage of DNA sequences to regulatory sequences is typicallyaccomplished by ligation at suitable restriction sites or adapters orlinkers inserted in lieu thereof using restriction endonucleases knownto one of skill in the art.

In a particular embodiment the polynucleotides or homologues or variantsor fragments thereof of the present invention can be introduced inplants or plant cells that are different from tobacco and thepolynucleotides can be used for the modulation of secondary metabolitesynthesis in plants or plant cells different from tobacco.

The term “heterologous DNA” and or “heterologous RNA” refers to DNA orRNA that does not occur naturally as part of the genome or DNA or RNAsequence in which it is present, or that is found in a cell or locationin the genome or DNA or RNA sequence that differs from that which isfound in nature. Heterologous DNA and RNA (in contrast to homologous DNAand RNA) are not endogenous to the cell into which it is introduced, buthas been obtained from another cell or synthetically or recombinantlyproduced. An example is a gene isolated from one plant species operablylinked to a promoter isolated from another plant species. Generally,though not necessarily, such DNA encodes RNA and proteins that are notnormally produced by the cell in which the DNA is transcribed orexpressed. Similarly exogenous RNA encodes for proteins not normallyexpressed in the cell in which the exogenous RNA is present.Heterologous DNA or RNA may also refer to as foreign DNA or RNA. Any DNAor RNA that one of skill in the art would recognize as heterologous orforeign to the cell in which it is expressed is herein encompassed bythe term heterologous DNA or heterologous RNA. Examples of heterologousDNA include, but are not limited to, DNA that encodes proteins,polypeptides, receptors, reporter genes, transcriptional andtranslational regulatory sequences, selectable or traceable markerproteins, such as a protein that confers drug resistance, RNA includingmRNA and antisense RNA and ribozymes.

In yet another embodiment, the invention provides for a method toidentify genes which expression modulates the production of at least onesecondary metabolite in an organism or cells derived thereof comprisingthe steps of (a) performing a genome wide expression profiling of theorganism or cells on different times of growth, (b) isolating geneswhich expression is co-regulated either with the at least one secondarymetabolite, or with a gene known to be involved in the biosynthesis ofthe secondary metabolite, (c) analysing the effect of over- orunder-expression of the genes in the organism or cell on the productionof the at least one secondary metabolite and (d) identifying genes thatcan modulate the production of the at least one secondary metabolite.

The wording “performing a genome wide expression profiling” means thatthe expression of genes and/or proteins is measured. Preferably, theexpression is measured on different times of growth, on differenttreatments and the like. Usually a comparison of the expression is madebetween two or more samples (e.g., samples that are treated andnon-treated, induced or non-induced). Gene expression can be measured byvarious methods known in the art comprising macro-array technology,micro-array technology, serial analysis of gene expression (SAGE), cDNAAFLP and the like. With array technology complete genes or partsthereof, EST sequences, cDNA sequences, oligonucleotides are attached toa carrier. Protein expression can be measured through various proteinisolation, protein profiling and protein identification methods known inthe art. The analysis of the effect of over- or under-expression ofgenes in for example plants or plant cells can be carried out by variouswell-known methods in the art.

In a further embodiment, the invention provides a method where theperformance of the genome wide expression profiling is preceded by thestep of inducing the production of the at least one secondary metabolitein the organism or cell derived thereof. The wording ‘inducing theproduction’ means that for example the cell culture, such as a plantcell culture, is stimulated by the addition of an external factor.External factors include the application of heat, the application ofcold, the addition of acids, bases, metal ions, fungal membraneproteins, sugars and the like. One approach that has been giveninteresting results for better production of plant secondary metabolitesis elicitation. Elicitors are compounds capable of inducing defenceresponses in plants (Darvil and Albersheim, 1984). These are usually notfound in intact plants but their biosynthesis is induced after woundingor stress conditions. Commonly used elicitors are jasmonates, mainlyjasmonic acid and its methyl ester, methyl jasmonate. Jasmonates arelinoleic acid derivatives of the plasma membrane and display a widedistribution in the plant kingdom (for overview see Reinbothe et al.,1994). They were originally classified as growth inhibitors or promotersof senescence but now it has become apparent that they have pleiotropiceffects on plant growth and development. Jasmonates appear to regulatecell division, cell elongation and cell expansion and thereby stimulateorgan or tissue formation (Swiatek et al., 2002). They are also involvedin the signal transduction cascades that are activated by stresssituations such as wounding, osmotic stress, desiccation and pathogenattack (Creelman et al., 1992; Gundlach et al., 1992; Ishikawa et al.,1994). Methyl jasmonate (MeJA) is known to induce the accumulation ofnumerous defence-related secondary metabolites (e.g., phenolics,alkaloids and sesquiterpenes) through the induction of genes coding forthe enzymes involved in the biosynthesis of these compounds in plants(Gundlach, et al., 1992; Imanishi et al., 1998; Mandujano-Chávez et al.,2000). Jasmonates can modulate gene expression from the(post)transcriptional to the (post)translational level, both in apositive as in a negative way. Genes that are upregulated are e.g.,defence and stress related genes (PR proteins and enzymes involved withthe synthesis of phytoalexins and other secondary metabolites) whereasthe activity of housekeeping proteins and genes involved withphotosynthetic carbon assimilation are down-regulated (Reinbothe et al.,1994). For example: the biosynthesis of phytoalexins and other secondaryproducts in plants can also be boosted up by signal molecules derivedfrom micro-organisms or plants (such as peptides, oligosaccharides,glycopeptides, salicylic acid and lipophilic substances) as well as byvarious abiotic elicitors like UV-light, heavy metals (Cu, VOSO4, Cd)and ethylene. The effect of any elicitor is dependent on a number offactors, such as the specificity of an elicitor, elicitor concentration,the duration of the treatment and growth stage of the culture.

Generally, secondary metabolites can be measured, intracellularly or inthe extracellular space, by methods known in the art. Such methodscomprise analysis by thin-layer chromatography, high pressure liquidchromatography, capillaryelectrophoresis, gas chromatography combinedwith mass spectrometric detection, radioimmuno-assay (RIA) and enzymeimmuno-assay (ELISA).

In yet another embodiment, the method to identify genes which expressionmodulates the production of at least one secondary metabolite in anorganism or cells derived thereof is used to identify genes that areinvolved in the alkaloid biosynthesis.

The definition of “Alkaloids”, of which more than 12,000 structures havebeen described already, includes all nitrogen-containing naturalproducts which are not otherwise classified as peptides, non-proteinamino acids, amines, cyanogenic glycosides, glucosinolates, cofactors,phytohormones or primary metabolites (such as purine and pyrimidinebases). The “calystegins” constitute a unique subgroup of the tropanealkaloid class (Goldmann et al. (1990) Phytochemistry, 29, 2125). Theyare characterized by the absence of an N-methyl substituent and a highdegree of hydroxylation. Trihydroxylated calystegins are summarized asthe calystegin A-group, tetrahydroxylated calystegins as the B-group,and pentahydroxylated derivates form the C-group. Calystegins representa novel structural class of tropane alkaloids possessing potentglycosidase inhibitory properties next to longer known classes of themonocyclic pyrrolidones (e.g., dihydroxymethyldihydroxy pyrrolidine)pyrrolines and piperidines (e.g., deoxynojirimycin), and the bicyclicpyrrolizidines (e.g., australine) and indolizidines (e.g., swainsonineand castanospermine). Glycosidase inhibitors are potentially useful asantidiabetic, antiviral, antimetastatic, and immunomodulatory agents.

In another embodiment, the method to identify genes which expressionmodulates the production of at least one secondary metabolite in anorganism or cells derived thereof is used to identify genes that areinvolved in the phenylpropanoid biosynthesis. “Phenylpropanoids” or“phenylpropanes” are aromatic compounds with a propyl side-chainattached to the aromatic ring, which can be derived directly fromphenylalanine. The ring often carries oxygenated substituents (hydroxyl,methoxyl and methylenedioxy groups) in the para-position. Naturalproducts in which the side-chain has been shortened or removed can alsobe derived from typical phenylpropanes. Most plant phenolics are derivedfrom the phenylpropanoid and phenylpropanoid-acetate pathways and fulfila very broad range of physiological roles in plants. For examplepolymeric lignins reinforce specialized cell wall. Closely related arethe lignans which vary from dimers to higher oligomers. Lignans caneither help defend against various pathogens or act as antioxidants inflowers, leaves and roots. The flavonoids comprise an astonishinglydiverse group of more than 4500 known compounds. Among their subclassesare the anthocyanins (pigments), proanthocyanidins or condensed tannins(feeding deterrents and wood protectants), and isoflavonoids (defensiveproducts and signalling molecules). The coumarins, furanocoumarins, andstilbenes protect against bacterial and fungal pathogens, discourageherbivory, and inhibit seed germination.

In yet another embodiment, the isolated polynucleotides of theinvention, or homologues, or variants, or fragments thereof are used tomodulate the biosynthesis of secondary metabolites in an organism orcell derived thereof. In a particular embodiment the isolatedpolynucleotides, homologues, variants or fragments thereof are used tomodulate the biosynthesis of secondary metabolites in plants or plantcells derived thereof.

In yet another embodiment, the polynucleotides comprising SEQ ID NO: 10,11, 19, 20, 35, 40, 41, 47, 65, 67, 70, 88, 89, 97, 98, 101, 102, 103,106, 107, 108, 117, 118, 120, 121, 123, 124, 126, 128, 130, 131, 132,136, 137, 142, 143, 144, 145, 146, 147, 148, 152, 154, 155, 159, 160,161, 162, 163, 175, 176, 177, 181, 182, 183, 189, 197, 202, 207, 208,209, 210, 217, 219, 220, 221, 233, 235, 236, 237, 239, 240, 241, 242,243, 244, 261, 262, 264, 265, 268, 70, 272, 273, 274, 278, 279, 299,300, 302, 303, 304, 305, 306, 316, 317, 318, 320, 321, 326, 329, 331,332, 333, 334, 341, 344, 348, 349, 350, 351, 354, 355, 356, 358, 372,373, 374, 375, 377, 382, 390, 391, 392, 395, 403, 405, 406, 414, 417,418, 419, 420, 424, 430, 434, 439, 440, 441, 445, 446, 456, 463, 478,485, 491, 497, 507, 508, 510, 518, 519, 527, 529, 531, 532, 534, 567,569, 570, 575, 577, 579, 587, 593, 594, 598, 599, 601, 603, 608, 612,613, 618, 619, 620, 628, 636, 642, 643, 647, 648, 649, 652, 653, 654,655, 656, 657, 659, 660, 662, 664, 670, 671, 674, 675, 676, 677, 679,680, 682, 683, 695, 696, 700, 701, 703, 707, 709, 710, 711, 712, 714,719, 724, 727, 729, 732, 734, 735, 740, 741, 744, 746, 748, 749, 750,751, 753, 754, 755, 757, 758, 759, 760, 761, 762, 763, 764, 766, 767,772, 777, 784, 794, 809, 810, 811, 816, 817, 822, 823, 826, 827, 828,829, 830, 832, 833, 834, 836, 837, 839, 840, 841, 850, 854, 855, 856,858, 859, 861, 864, 865, 488, 489 and/or 490 or fragments or homologuesthereof can be used to modulate the biosynthesis of alkaloids in anorganism or cell derived thereof. In a particular embodiment thepolynucleotides or fragments or homologues thereof can be used tomodulate the biosynthesis of alkaloids in plants or plant cells derivedthereof. The expression of the latter collection of SEQ ID Numberscorrelates with the production of alkaloids in plants.

In yet another embodiment, the polynucleotides comprising SEQ ID NO: 3,4, 5, 7, 15, 17, 21, 23, 29, 30, 32, 33, 39, 42, 44, 45, 46, 48, 49, 50,51, 8, 61, 62, 72, 74, 79, 84, 92, 94, 95, 104, 105, 125, 134, 150, 170,171, 179, 180, 184, 194, 195, 200, 201, 203, 204, 205, 213, 214, 215,218, 245, 249, 250, 251, 252, 254, 255, 266, 275, 276, 281, 282, 285,286, 287, 289, 291, 298, 301, 308, 309, 310, 311, 312, 313, 315, 319,323, 324, 335, 343, 361, 363, 364, 370, 379, 380, 383, 384, 385, 386,398, 401, 402, 407, 415, 416, 423, 432, 433, 437, 443, 444, 447, 448,450, 451, 452, 455, 457, 460, 461, 462, 471, 474, 486, 487, 493, 494,499, 500, 501, 502, 503, 504, 505, 506, 517, 522, 523, 524, 526, 528,538, 541, 543, 544, 545, 546, 547, 553, 554, 555, 562, 568, 571, 572,578, 580, 581, 582, 588, 605, 607, 616, 617, 621, 626, 627, 637, 638,641, 644, 650, 651, 665, 666, 667, 681, 684, 685, 691, 697, 698, 704,708, 713, 720, 721, 728, 730, 736, 745, 752, 756, 771, 776, 778, 782,783, 792, 793, 795, 797, 798, 799, 800, 801, 808, 815, 818, 819, 820,821, 835, 842, 843, 844, 845, 848, 851, 852, 853, 862, 868, 488, 489and/or 490 or fragments or homologues thereof can be used to modulatethe biosynthesis of phenylpropanoids in an organism or cell derivedthereof. In a particular embodiment the polynucleotides or homologues orfragments derived thereof can be used to modulate the biosynthesis ofphenylpropanoids in plants or plant cells derived thereof. Theexpression of the latter collection of SEQ ID Numbers correlates withthe production of phenylpropanoids in plants.

The present invention can be practiced with any plant variety for whichcells of the plant can be transformed with an expression cassette of thecurrent invention and for which transformed cells can be cultured invitro. Suspension culture, callus culture, hairy root culture, shootculture or other conventional plant cell culture methods may be used (asdescribed in: Drugs of Natural Origin, G. Samuelsson, 1999, ISBN9186274813).

By “plant cells” it is understood any cell which is derived from a plantand can be subsequently propagated as callus, plant cells in suspension,organized tissue and organs (e.g., hairy roots). In the presentinvention the word “plant cell” also comprises cells derived from lowerplants such as from the Pteridophytae and the Bryophytae.

Tissue cultures derived from the plant tissue of interest can beestablished. Methods for establishing and maintaining plant tissuecultures are well known in the art (see, for example, Trigiano R. N. andGray D. J. (1999), “Plant Tissue Culture Concepts and LaboratoryExercises”, ISBN: 0-8493-2029-1; Herman E. B. (2000), “Regeneration andMicropropagation: Techniques, Systems and Media 1997-1999”, AgricellReport). Typically, the plant material is surface-sterilized prior tointroducing it to the culture medium. Any conventional sterilizationtechnique, such as chlorinated bleach treatment can be used. Inaddition, antimicrobial agents may be included in the growth medium.Under appropriate conditions plant tissue cells form callus tissue,which may be grown either as solid tissue on solidified medium or as acell suspension in a liquid medium.

A number of suitable culture media for callus induction and subsequentgrowth on aqueous or solidified media are known. Exemplary media includestandard growth media, many of which are commercially available (e.g.,Sigma Chemical Co., St. Louis, Mo.). Examples include Schenk-Hildebrandt(SH) medium, Linsmaier-Skoog (LS) medium, Murashige and Skoog (MS)medium, Gamborg's B5 medium, Nitsch & Nitsch medium, White's medium, andother variations and supplements well known to those of skill in the art(see, for example, Plant Cell Culture, Dixon, ed. IRL Press, Ltd. Oxford(1985) and George et al., Plant Culture Media, Vol 1, Formulations andUses Exegetics Ltd. Wilts, UK, (1987)). For the growth of conifer cells,particularly suitable media include 1/2 MS, 1/2 L. P., DCR, Woody PlantMedium (WPM), Gamborg's B5 and its modifications, DV (Durzan andVentimiglia, In Vitro Cell Dev. Biol. 30:219-227 (1994)), SH, andWhite's medium.

In a particular embodiment, the current invention can be combined withother known methods to enhance the production and/or the secretion ofsecondary metabolites in plant cell cultures such as (1) by improvementof the plant cell culture conditions, (2) by the transformation of theplant cells with a transcription factor capable of upregulating genesinvolved in the pathway of secondary metabolite formation, (3) by theaddition of specific elicitors to the plant cell culture, and 4) by theinduction of organogenesis.

The term “plant” as used herein refers to vascular plants (e.g.,gymnosperms and angiosperms). The method comprises transforming a plantcell with an expression cassette of the present invention andregenerating such plant cell into a transgenic plant. Such plants can bepropagated vegetatively or reproductively. The transforming step may becarried out by any suitable means, including by Agrobacterium-mediatedtransformation and non-Agrobacterium-mediated transformation, asdiscussed in detail below. Plants can be regenerated from thetransformed cell (or cells) by techniques known to those skilled in theart. Where chimeric plants are produced by the process, plants in whichall cells are transformed may be regenerated from chimeric plants havingtransformed germ cells, as is known in the art. Methods that can be usedto transform plant cells or tissue with expression vectors of thepresent invention include both Agrobacterium and non-Agrobacteriumvectors. Agrobacterium-mediated gene transfer exploits the naturalability of Agrobacterium tumefaciens to transfer DNA into plantchromosomes and is described in detail in Gheysen, G., Angenon, G. andVan Montagu, M. 1998. Agrobacterium-mediated plant transformation: ascientifically intriguing story with significant applications. In K.Lindsey (Ed.), Transgenic Plant Research. Harwood Academic Publishers,Amsterdam, pp. 1-33 and in Stafford, H. A. (2000) Botanical Review 66:99-118. A second group of transformation methods is thenon-Agrobacterium mediated transformation and these methods are known asdirect gene transfer methods. An overview is brought by Barcelo, P. andLazzeri, P. A. (1998) Direct gene transfer: chemical, electrical andphysical methods. In K. Lindsey (Ed.), Transgenic Plant Research,Harwood Academic Publishers, Amsterdam, pp. 35-55. Hairy root culturescan be obtained by transformation with virulent strains of Agrobacteriumrhizogenes, and they can produce high contents of secondary metabolitescharacteristic to the mother plant. Protocols used for establishing ofhairy root cultures vary, as well as the susceptibility of plant speciesto infection by Agrobacterium (Toivounen L. (1993) Biotechnol. Prog. 9,12; Vanhala L. et al. (1995) Plant Cell Rep. 14, 236). It is known thatthe Agrobacterium strain used for transformation has a great influenceon root morphology and the degree of secondary metabolite accumulationin hairy root cultures. It is possible that by systematic cloneselection e.g., via protoplasts, to find high yielding, stable, and fromsingle cell derived-hairy root clones. This is possible because thehairy root cultures possess a great somaclonal variation. Anotherpossibility of transformation is the use of viral vectors (Turpen T H(1999) Philos Trans R Soc Lond B Biol Sci 354(1383): 665-73).

Any plant tissue or plant cells capable of subsequent clonalpropagation, whether by organogenesis or embryogenesis, may betransformed with an expression vector of the present invention. The term‘organogenesis’ means a process by which shoots and roots are developedsequentially from meristematic centers; the term ‘embryogenesis’ means aprocess by which shoots and roots develop together in a concertedfashion (not sequentially), whether from somatic cells or gametes. Theparticular tissue chosen will vary depending on the clonal propagationsystems available for, and best suited to, the particular species beingtransformed. Exemplary tissue targets include protoplasts, leaf disks,pollen, embryos, cotyledons, hypocotyls, megagametophytes, callustissue, existing meristematic tissue (e.g., apical meristems, axillarybuds, and root meristems), and induced meristem tissue (e.g., cotyledonmeristem and hypocotyls meristem).

These plants may include, but are not limited to, plants or plant cellsof agronomically important crops, such as tomato, tobacco, diverse herbssuch as oregano, basilicum and mint. It may also be applied to plantsthat produce valuable compounds, for example, useful as for instancepharmaceuticals, as ajmalicine, vinblastine, vincristine, ajmaline,reserpine, rescinnamine, camptothecine, ellipticine, quinine, andquinidine, taxol, morphine, scopolamine, atropine, cocaine,sanguinarine, codeine, genistein, daidzein, digoxin, calystegins or asfood additives such as anthocyanins, vanillin; including but not limitedto the classes of compounds mentioned above. Examples of such plantsinclude, but not limited to, Papaver spp., Rauwolfia spp., Taxus spp.,Cinchona spp., Eschscholtzia californica, Camptotheca acuminata,Hyoscyamus spp., Berberis spp., Coptis spp., Datura spp., Atropa spp.,Thalictrum spp., Peganum spp.

In yet another embodiment, suitable expression cassettes comprising thenucleotide sequences of the present invention can be used fortransformation into other species (different from Tobacco). Thistransformation into other species or genera (different from the genusNicotiana) can be carried out randomly or can be carried out withstrategically chosen nucleotide sequences. The random combination ofgenetic material from one or more species of organisms can lead to thegeneration of novel metabolic pathways (for example through theinteraction with metabolic pathways resident in the host organism oralternatively silent metabolic pathways can be unmasked) and eventuallylead to the production of novel classes of compounds. This novel orreconstituted metabolic pathways can have utility in the commercialproduction of novel, valuable compounds.

The recombinant DNA and molecular cloning techniques applied in thebelow examples are all standard methods well known in the art and are,for example, described by Sambrook et al. (1989) Molecular cloning: Alaboratory manual, second edition, Cold Spring Harbor Laboratory Press.Methods for tobacco cell culture and manipulation applied in the belowexamples are methods described in or derived from methods described inNagata et al. (1992) Int. Rev. Cytol. 132, 1.

The invention is further explained with the aid of the followingillustrative examples.

EXAMPLES

1) Nicotine Alkaloids

First, the identification of various tobacco alkaloids: nicotine,nornicotine, anatabine, myosmine, anabasine and N′-formylnornicotine wasdetermined from leaves, where the occurrence of alkaloids is abundant.Identification was based on the GC-MS spectra and literature (see, FIG.3). There were no alkaloids detected in the control samples of BY-2.Elicitation of BY-2 cells by methyl jasmonate leads to a marked increasein nicotine, anabasine, anatalline, and especially in anatabine content,the latter clearly being the main component (FIGS. 4 & 5). To ourknowledge, this is the first time that besides nicotine, these otheralkaloids has been detected in tobacco BY-2 cell cultures.

Elicitation with methyl jasmonate seems to induce the pathway throughnicotinic acid (FIG. 1). Especially the concentration of anatabine wasraised, which according to literature based on biosynthetic studies, issimply derived from nicotinic acid, but neither through the argininepathway, which leads to nicotine, nor via the lysine pathway which, inturn, leads to anabasine. The elicited BY-2 samples also containedincreased amounts of two isomeric alkaloids with m/z 239 as themolecular ion. It is called anatalline and it has been discoveredearlier only in the roots of N. tabacum, and never in cell cultures. Yetit was not detected in tobacco leaves. Anatalline is composed of threepyridine ring units of which one has no double bonds(2,4-bis-3′-pyridyl-piperidine). Based on the mass spectra, anatallinemay not be derived from anatabine, but rather from anabasine. This isalso in accordance with the information found in the literature. In thegrowth medium of BY-2 cells no alkaloids could be detected.

The elicitation with methyl jasmonate induces the accumulation ofvarious nicotine alkaloids. The accumulation of alkaloid metabolites inthe cells started after 14 hours and reached their maximum levelstowards the end of the experimental period (FIG. 6). The accumulation ofnicotine and anatabine started to take place after 14 and 24 hours,respectively. The contents of anabasine, and two isomers of anatallinein the cells increased only after 48 hours. The maximum concentration ofnicotine was only 4% (on dry weight basis) of that of the main alkaloidanatabine, which reached the highest concentration of 800 μg/g (d.w.).The time-course of the onset of nicotine accumulation is in accordancewith the data reported by Imanishi et al. (1998), who studied onlynicotine alkaloid pattern after elicitation. Anatabine and nicotine aresynthesized first, while anabasine and anatalline, which follow exactlythe similar time-course patterns, accumulate later (FIG. 6).

Instead of nicotine, the level of alkaloids on the other branch of thebiosynthetic pathway, for example, anatabine and anatalline wasremarkably raised, both branches competing for the supply of nicotinicacid. This was the first time that anatalline was found to besynthesised in the cell suspension cultures of tobacco. The resultindicates that nicotine, having two precursors, nicotinic acid andN-methylpyrrolinium, might not be synthesised if the latter is alimiting factor. Thus the pathway from nicotinic acid is directedtowards the other biosynthetic routes (see FIG. 1).

2) Polyanines

The detection of various polyamines in BY-2 cells including spermidine,spermine, putrescine and methylputrescine were detected by HPLC(Scaramagli et al., 1999). In free pool there were no significantchanges between elicited and control samples, except for methylputrescine which accumulates dramatically in elicited cells (FIG. 7,FIG. 8). Soluble conjugates, which are amines conjugated with phenolicacid, mainly cinnamic acid derivatives did not change much except formethyl putrescine, which accumulates in elicited cells from 12 hoursonwards (FIG. 9). Insoluble conjugates which are mainly polyaminesassociated in cell walls showed that especially putrescine and alsomethyl putrescine accumulate in elicited cells (FIG. 10). In short, itseems that elicitor treatment induces the accumulation of intermediatesputrescine and methyl putrescine in nicotine pathway.

3) Sesquiterpenes

The preliminary experiment indicated the presence of various oxygenatedsesquiterpenoid alkaloids, detected in the elicitated cells of tobaccoBY-2. Presumably they are structurally aristolochene-likesesquiterpenes, with the molecular weight of 224. Aristolochenes arecompounds found in the early steps of the biosynthetic pathway ofsesquiterpenes, for example, capsidiol, lubimine, solavetivone,phytuberin and phytuberol.

4) Phenylpropanoids

TLC analysis of BY-2 cells and culture filtrates clearly shows thatapart form nicotine, jasmonates also are able to induce the productionof (several) phenylpropanoid-like substances.

5) Quantitative Analysis of Jasmonate-Modulated Gene Expression

By using the combination of metabolic profiling and cDNA-AFLP basedtranscript profiling of jasmonate-elicited tobacco BY-2 cells we wereable to build an ample inventory of genes involved in plant secondarymetabolism and other jasmonate-regulated cellular events. The growthcurve of tobacco BY-2 cells is shown in FIG. 2. The culture wasinoculated as every 7th day subculturing, 1:100. The growth reached theexponential phase in 6 days. Stationary phase was obtained after 10days. The gene platform that was generated correlates also with earlierreports and reviews on jasmonate-modulated cellular and metabolicevents, pointing to the accuracy and the reliability of the profilinganalysis. Examples are the observed up-regulation of genes involved inthe biosynthesis of jasmonates (an auto-regulatory event) and genesinvolved in defense responses such as proteinase inhibitors andtransposases. At the same time numerous novel genes, either withoutexisting homologues or with homologues of known or unknown function,were identified as jasmonate responsive and correlates with theproduction of alkaloids and phenylpropanoids. Some of them point tocellular or metabolic events that have been not related with jasmonatesbefore.

Tobacco BY-2 cells were elicited with 50 μM methyl jasmonate andtranscript profiles were compared with the transcript profiles ofDMSO-treated cells. Quantitative temporal accumulation patterns ofapproximately 20,000 transcript tags were determined and analyzed. Intotal, 591 differential transcript tags were obtained. Sequencing of thePCR products gave good-quality sequences for approximately 80% of thefragments. To the remaining 20%, a unique sequence could notunambiguously be attributed because the fragments were contaminated withco-migrating bands. These bands have been cloned and PCR products fromfour individual colonies were sequenced. For most of these fragments,two to three different sequences were obtained from the individualcolonies. Homology searches with the sequences from the unique gene tagsrevealed that 64% of these tags displayed similarity with genes of knownfunctions, and 18% of the tags matched a cDNA or genomic sequencewithout allocated function. In contrast, no homology with a knownsequence was found for 18% of the tags.

By average linkage hierarchical clustering of the expression profiles,the genes could be grouped in two main clusters: induced and repressedby jasmonate elicitation. The group of jasmonate repressed genescomprises ca. 18% of the isolated gene tags. The vast majority ofjasmonate modulated genes is upregulated by jasmonate elicitation andcan be subdivided in three categories: early induced (within 1 hourafter the elicitation), intermediate (after two to 4 hours) and lateinduced (after 6 hours or more). These subcategories respectivelycomprise ca. 31%, 27% and 24% of the isolated gene tags.

Among the early induced subgroup figure, all the genes that are known tobe involved with nicotine biosynthesis in Nicotiana species, i.e.,arginine decarboxylase (ADC), ornithine decarboxylase (ODC) andquinolate phosphoribosyltransferase (QPRT). The fourth gene known to beinvolved in nicotine biosynthesis, putrescine methyl transferase (PMT),could not be picked up with the cDNA-AFLP method used here as itsnucleotide sequence does not harbor a BstYI restriction site.Nonetheless, RT-PCR analysis clearly shows that PMT expression is alsoupregulated as early as one hour after jasmonate treatment and thusdemonstrates the co-regulation of the PMT gene(s) with the othernicotine metabolic genes mentioned above. Interestingly, two other genetags coregulated with the above mentioned genes show homology withputative (amine) oxidases and potentially encode the still undiscoveredmethyl putrescine oxidase (MPO). Other gene tags that are found in thissubgroup are the genes involved with jasmonate biosynthesis such asallene oxide synthase, allene oxide cyclase, 12-oxophytodienoatereductase and lipoxygenases.

In the subsequent induction wave (within two to four hours) anothergroup of genes is found that putatively encode enzymes involved inflavonoid metabolism. Amongst these figure phenylalanine ammonia-lyase,chalcone synthase-like proteins, isoflavone synthase-like proteins,leucoanthocyanidin dioxygenase-like proteins and various cytochrome P450enzymes.

6) Functional Analysis of Candidate Genes.

Selected genes were introduced in appropriate vectors forover-expression and/or down-regulation using the Gateway™ technology(InVitrogen Life Technologies). To this end a set of Gateway compatiblebinary vectors for plant transformation was developed (Karimi et al.,2002). For over-expression the pK7WGD2 vector is used in which the geneis put under the control of the p35S promoter. Down-regulation is basedon the post-transcriptional gene silencing effect (PTGS, Smith et al.,2000) and to this end the pK7GWIWG2 is used. For plant celltransformations the ternary vector system (van der Fits et al., 2000)was applied. The plasmid pBBR1MCS-5.virGN54D was used as a ternaryvector. The binary plasmid was introduced into Agrobacterium tumefaciensstrain LBA4404 already bearing the ternary plasmid byelectro-transformation. For hairy root transformation the binary plasmidwas introduced in the Agrobacterium rhizogenes strain LBA9402.

Fresh BY-2 culture was established before the transformation with theparticular construct. Five-day-old BY-2 was inoculated 1:10 and grownfor three days (28° C., 130 rpm, dark). The liquid culture ofAgrobacterium tumefaciens transformed with pK7WGD2-GUS, pK7WGD2-NtCYP1(insert from SEQ ID No 465) or pK7WGD2-NtORC1 (insert from SEQ ID No285) was established two days before the transformation of BY-2. Aloopfull of bacteria from the solid medium was inoculated in 5 ml ofliquid LB medium with the antibiotics (rifampicin, gentamycin,streptomycin and spectinomycin). The culture was grown for two days (28°C., 130 rpm).

The transformation of BY-2 was performed in empty petri dish (Ø4.6 cm)with the cocultivation method. Three-day-old BY-2 (3 ml) was pipettedinto plate and either 50 or 200 μl of bacterial suspension was added.The plates were gently mixed and left to stand in the laminar bench inthe dark for three days. After cocultivation the cells were plated onthe solid BY-2-medium with the selections (50 μg/ml kanamycin, and 500μg/ml vancomycin and 500 μg/ml carbenicillin to kill the excess ofbacteria). The plates were sealed with millipore tape and incubated at28° C. in the dark for approximately two weeks after which the callibecame visible. The transformation was visualised by checking theexpression of GFP (green fluorescent protein) under the microscope.

The suspension culture of the transformed BY-2 was started by taking aclumb of calli (appr. Ø 1 cm) into 20 ml liquid BY-2 medium with theselection. After several subcultures the suspension volume wasincreased. When the growth of the culture reached the normal growthpattern of BY-2 (subculturing every 7th day), the elicitation experimentwas performed as described earlier. Before washing the culture in thebeginning of the experiment, the selection (kanamycin) was stillpresent. The density of the culture as well as the GFP expression andviability of the cells were checked before starting the experiment.

The nicotine alkaloids were detected 24 h and 48 h after elicitationwith MeJA (50 μM). Trace amounts of nicotine was detected in all samplesand no effect of transformed constructs (pK7WGD2-NtCYP1 andpK7WGD2-NtORC1) compared to the control (pK7WGD2-GUS) was observed (FIG.11). Anabasine concentration increased in a function of time and amarked increase compared to the control was observed withpK7WGD2-NtORC1-transformed line, bearing the ORCA homologue gene (FIG.12). Considering the major alkaloid anatabine, no difference in alkaloidaccumulation was observed 24 h after elicitation, but at 48 h bothtransformed constructs, bearing either cyclophilin or AP2 transcriptionfactor, showed clear increase in anatabine levels compared to thecontrol (FIG. 13). The two anatalline isomers followed the similarpattern as anatabine, the transformed lines bearing the putativelyfunctional constructs accumulated notably higher levels of both isomersthan the control line (FIG. 14). The overall levels of accumulatedalkaloids were in each transformed line lower than in untransformedBY-2, suggesting that the transformation protocol itself might have aninhibitory effect on alkaloid production. The effect of excess ofantibiotics possibly still present during the elicitation is also to betested for their contribution to lower accumulation of alkaloids.However, these results indicate that the above mentioned constructs hada considerable positive effect on the alkaloid accumulation compared tothe control line, bearing no functional construct.

7) Isolation of Full-Length Genes and Homologues

-   -   MAP3 (SEQ ID NO: 285 and SEQ ID NO: 872): sequence information        for an AP2-domain transcription factor, induced after 1 hour by        methyl jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx 3e-22):    -   emb|CAB96899.1| AP2-domain DNA-binding protein [Catharanthus        roseus]    -   emb|CAB93940.1| AP2-domain DNA-binding protein [Catharanthus        roseus]    -   gb|AAM45475.1| ethylene-responsive element binding protein 1        [Glycine max]    -   ref|NP_(—)182011.1| putative ethylene response element binding        protein (EREBP) At2g44840 [Arabidopsis thaliana]    -   pir∥T02432 ethylene-responsive transcription factor ERF1        [Nicotiana tabacum]    -   pir∥T07686 transcription factor Pti4 [Lycopersicon esculentum]    -   C330 (SEQ ID NO: 148 and SEQ ID NO: 873): sequence information        for an AP2-domain transcription factor induced after 1 hour by        methyl jasmonate in tobacco BY-2 cells.        Best Homologues found:(lowest blastx 2e-27):    -   ref|NP_(—)199533.1| ethylene responsive element binding factor 2        (EREBP-2) [A. thaliana]    -   dbj|BAA87068.2| ethylene-responsive element binding protein1        homolog [Matricaria chamomilla]    -   gb|AAF63205.1| AF245119_(—)1 AP2-related transcription factor        [Mesembryanthemum crystallinum]    -   pir∥T07686 transcription factor Pti4 [Lycopersicon esculentum]    -   pir∥T02590 ethylene-responsive element binding protein        [Nicotiana tabacum]        Both MAP3 and C330 encode transcription factors belonging to the        AP2-domain transcription factor family, to which also for        instance the ORCA genes belong, known to regulate the jasmonate        responsive biosynthesis of terpenoid indole alkaloids in        Catharanthus roseus (Memelink et al., Trends Plant Sci. 2001,        6(5):212-219). Since both MAP3 and C330 are induced before or        concomitantly with the nicotine biosynthetic genes PMT, ADC,        ODC, QPRT, AP and SAMS, this clearly mirrors a potential role as        activators of nicotine biosynthesis for these genes. This was        confirmed by assessment of nicotine alkaloid accumulation levels        (for MAP3 and reporter gene expression analysis (for C330).    -   C484a (SEQ ID N° 275 and SEQ ID NO: 874): a C3HC4-type RING zinc        finger protein induced after 1 hour by methyl jasmonate in        tobacco BY-2 cells.        Best Homologues found: (lowest blastx 8e-30)>    -   ref|NP_(—)181135.2| putative RING zinc finger protein At2g35910        [A. thaliana]    -   ref|NP_(—)196267.1| C3HC4-type RING zinc finger protein        At5g06490 [A. thaliana]        Zinc finger proteins can be transcriptional regulators reported        to interact for instance with the promoter regions of some genes        involved in the biosynthesis of terpenoid indole alkaloids in        Catharanthus roseus (Ouwerkerk et al., Mol. Gen. Genet. 1999,        261(4-5):610-622). They can also interact with components of the        SCF (Skp1/Cullin/F-box protein)-type E3 ubiquitin ligase complex        involved in protein degradation (e.g., Liu et al, Plant Cell        2002, 14(7):1483-1496). Such a complex has shown to be of        extreme importance in jasmonate-mediated signaling cascades        (Turner et al., Plant Cell. 2002, 14 Suppl:S153-S164) and thus        participates as well in the regulation of plant secondary        metabolism.        C360 (SEQ ID NO: 180 and SEQ ID NO: 875): sequence information        for a protein with similarity to the putative protein At4g14710        [A. thaliana] induced after 4 hour by methyl jasmonate in        tobacco BY-2 cells.        Best Homologues found: (lowest blastx 2e-87)>    -   ref|NP_(—)567441.1| Expressed protein At4g14710 [A. thaliana]    -   ref|NP-567443.1| Expressed protein At4g14716 [A. thaliana]    -   ref|NP_(—)180208.1| unknown protein At2g26400 [A. thaliana]    -   pir∥T02918 probable submergence induced, nickel-binding protein        2A [Oryza sativa]    -   dbj|BAB61039.1| iron-deficiency induced gene [Hordeum vulgare]    -   >pir∥T02787 probable submergence induced protein 2 [Oryza        sativa]

This protein contains an ARD/ARD′ family motif, found in twoacireductone dioxygenase enzymes (ARD and ARD′, previously known as E-2and E-2′) from Klebsiella pneumoniae. The two enzymes share the samesubstrate, 1,2-dihydroxy-3-keto-5-(methylthio)pentene, but yielddifferent products. ARD′ yields the alpha-keto precursor of methionine(and formate), thus forming part of the ubiquitous methionine salvagepathway that converts 5′-methylthioadenosine (MTA) to methionine. Thispathway is responsible for the tight control of the concentration ofMTA, which is a powerful inhibitor of polyamine biosynthesis andtransmethylation reactions [1,2]. ARD yields methylthiopropanoate,carbon monoxide and formate, and thus prevents the conversion of MTA tomethionine. The role of the ARD catalysed reaction is unclear:methylthiopropanoate is cytotoxic, and carbon monoxide can activateguanylyl cyclase, leading to increased intracellular cGMP levels (Duaiet al., J. Biol. Chem. 1999, 274(3):1193-1195; Dai et al., Biochemistry2001, 40(21):6379-6387). This family also contains other members, whosefunctions are not well characterized. The gene isolated here mightprobably regulate/interact with polyamine biosynthesis and thus nicotinebiosynthesis, for which polyamines are precursors.

-   -   C165 (SEQ ID NO: 64 and SEQ ID NO: 876): sequence information        for a putative ligand-gated ion channel protein induced after 6        hour by methyl jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx 2e-80)>    -   ref|NP_(—)172012.1| putative ligand-gated ion channel protein        At1g05200 [A. thaliana]    -   ref|NP_(—)565743.1| putative ligand-gated ion channel protein        At2g32390 [A. thaliana]    -   dbj|BAC57657.1| putative ionotropic glutamate receptor homolog        GLR4 [Oryza sativa (japonica cultivar-group)]    -   dbj|BAC10393.1| putative ligand-gated channel-like protein        [Oryza sativa (japonica cultivar-group)]        Ligand-gated ion channels are important players in plant hormone        induced signaling cascades. They have been found to be involved        for instance in abscisic acid signalling (Pei et al., Nature        2000, 406(6797):731-734; Walden, Curr. Opin. Plant Biol. 1998,        1(5):419-423). Abscisic acid, as well as ethylene and jasmonates        have also been proposed to play a role in wound signalling,        which in many plants leads to the induction of plant secondary        metabolic pathways (Leon et al., J. Exp. Bot. 2001 52(354):1-9).    -   C353a (SEQ ID NO: 172 and SEQ ID NO: 877): sequence information        for a GTP-binding protein induced after 6 hour by methyl        jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx e-102)>    -   emb|CAA69701.1| small GTP-binding protein [Nicotiana        plumbaginifolia]    -   emb|CAC39050.1| putative GTP-binding protein [Oryza sativa]    -   dbj|BAA76422.1| rab-type small GTP-binding protein [Cicer        arietinum]    -   emb|CAA98160.1| RAB1C [Lotus japonicus]    -   pir∥B38202 GTP-binding protein YPTM2 [Zea Mays]    -   dbj|BAA02116.1| GTP-binding protein [Pisum sativum]    -   emb|CAA98161.1| RAB1D [Lotus japonicus]    -   gb|AAF65510.1| small GTP-binding protein [Capsicum annuum]    -   emb|CAA98162.1| RAB1E [Lotus japonicus]    -   ref|NP_(—)193486.1| ras-related small GTP-binding protein RAB1c        At4g17530.1 [A. thaliana]    -   MT101 (SEQ ID NO: 355 and SEQ ID NO: 878): Sequence information        for a GTP-binding-like protein induced after 1 hour by methyl        jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx e-177)>    -   ref|NP_(—)195662.1| GTP-binding-like protein; protein id:        At4g39520.1 [A. thaliana]    -   dbj|BAC22346.1| putative GTP-binding protein [Oryza sativa        (japonica cultivar-group)]        GTP-binding proteins have been reported to be involved in the        induction of phytoalexin biosynthesis in cultured carrot cells        (Kurosaki et al., Plant Sci. 2001 161(2):273-278) and in the        fungal elicitor-induced beta-thujaplicin biosynthesis in        Cupressus lusitanica cell cultures (Zhao & Sakai, J. Exp. Bot.        2003, 54(383):647-656). They are supposed to interact with        receptors, kinases and phosphatases amongst others and as such        participate in many stimulus induced signaling pathways in        plants (Clark et al., Curr. Sci. 2001, 80(2):170-177), and        possibly as well in the onset of secondary metabolite        biosynthetic pathways.    -   T21 (SEQ ID NO: 465 and SEQ ID NO: 879): Sequence information        for a cyclophilin induced after 8 hour by methyl jasmonate in        tobacco BY-2 cells.        Best Homologues found: (lowest blastx 4e-78)>    -   gb|AAA63543.1| cyclophilin [Lycopersicon esculentum]    -   >pir∥CSTO peptidylprolyl isomerase (EC 5.2.1.8) [Lycopersicon        esculentum]    -   >pir∥T50771 peptidylprolyl isomerase (EC 5.2.1.8) [Solanum        tuberosum subsp. tuberosum]    -   emb|CAC80550.1| cyclophilin [Ricinus communis]    -   gb|AAB51386.1| stress responsive cyclophilin [Solanum        commersonii]    -   pir∥T50768 cyclophylin [Digitalis lanata]        Cyclophylins or FK506-binding proteins belong to the large        family of peptidyl-prolyl cis-trans isomerases, which are known        to be involved in many cellular processes, such as cell        signalling, protein trafficking and transcription (Harrar et        al., Trends Plant Sci 2001, 6(9):426-431), and as such might be        involved in regulating plant secondary metabolism.    -   C476a (SEQ ID NO: 264 and SEQ ID NO: 880): sequence information        for a MAP kinase induced after 1 hour by methyl jasmonate in        tobacco BY-2 cells.        Best Homologues found: (lowest blastx 2e-75)>    -   ref|NP_(—)177492.1| MAP kinase At1g73500 [A. thaliana]    -   ref|NP_(—)173271.1| MAP kinase kinase 5 At1g18350 [A. thaliana]    -   ref|NP_(—)188759.1| MAP kinasekinase 5 At3g21220 [A. thaliana]    -   ref|NP_(—)175577.1| MAP kinase kinase 4 (ATMKK4) At1g51660 [A.        thaliana]    -   gb|AAG53979.1|AF325168_(—)1 mitogen-activated protein kinase 2        [Nicotiana tabacum]        MAP kinases have been reported to be both differentially induced        by defense signals such as nitric oxide, salicylic acid,        ethylene, and jasmonic acid as to represent key components of        the signaling cascades induced by these defense signals (e.g.,        Petersen et al., Cell 2000, 103(7):1111-1120; Kumar & Klessig,        Mol. Plant Microbe Interact. 2000, 13(3):347-351; Seo et al.,        Science. 1995, 270(5244):1988-1992), and as such might be        involved in the activation of plant secondary metabolism.    -   MC204 (SEQ ID NO: 315 and SEQ ID NO: 881): sequence information        for a sequence with similarity to the putative protein At5g47790        [A. thaliana] induced after 6 hour by methyl jasmonate in        tobacco BY-2 cells.        Best Homologues found: (lowest blastx e-111)    -   dbj|BAC22308.1| OJ1136_A10.4 [Oryza sativa (japonica        cultivar-group)]    -   ref|NP_(—)199590.1| unknown protein At5g47790 [A. thaliana]        This protein contains a Forkhead-associated (FHA) domain. The        forkhead-associated domain is a phosphopeptide recognition        domain found in many regulatory proteins. It displays        specificity for phosphothreonine-containing epitopes but will        also recognize phosphotyrosine with relatively high affinity. It        spans approximately 80-100 amino acid residues folded into an        11-stranded sandwich, which sometimes contain small helical        insertions between the loops connecting the strands. The domain        is present in a diverse range of proteins, such as kinases,        phosphatases, kinesins, transcription factors, RNA-binding        proteins and metabolic enzymes which take part in many different        cellular processes, such as signal transduction, vesicular        transport and protein degradation (Durocher et al., Mol. Cell        1999, 4(3):387-394; Hofmann & Bucher, Trends Biochem. Sci. 1995,        20(9):347-349), and as such might regulate plant secondary        metabolism.    -   T323 (SEQ ID NO: 509 and SEQ ID NO: 882): Sequence information        for a putative endo-1,4-beta-glucanase induced after 10 hour by        methyl jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx 2e-84)>    -   emb|CAD41248.1| OSJNBa0067K08.12 [Oryza sativa (japonica        cultivar-group)]    -   ref|NP_(—)176738.1| glycosyl hydrolase family 9        (endo-1,4-beta-glucanase) At1g65610 [A. thaliana]    -   ref|NP_(—)199783.1| cellulase [A. thaliana]    -   emb|CAB51903.1| cellulase; endo-1,4-beta-D-glucanase [Brassica        napus]    -   pir∥T07612 cellulase [Lycopersicon esculentum]        The Arabidopsis mutant cev1 links cell wall signaling to        jasmonate and ethylene responses (Ellis et al., Plant Cell 2002,        14(7):1557-1566). CEV1 encodes a cellulose synthase. The cev1        mutant has constitutive expression of stress response genes and        has increased production of jasmonate and ethylene. Conversely,        as such glucanase and cellulase-like proteins might participate        in the onset of plant secondary metabolism by providing cell        wall derived molecules, necessary to elicit secondary metabolic        pathways.    -   T464 (SEQ ID NO: 595 and SEQ ID NO: 883): Sequence information        for an epimerase/dehydratase-like protein induced after 10 hour        by methyl jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx 0.0)>    -   gb|AAM08784.1|AC016780_(—)14 Putative epimerase/dehydratase        [Oryza sativa]    -   ref|NP_(—)198236.1| epimerase/dehydratase-like protein        At5g28840.1 [A. thaliana]        It has been shown that phytoalexin production elicited by        exogenously applied jasmonic acid in rice leaves (Oryza sativa        L.) is under the control of cytokinins and ascorbic acid        (Tamogami et al., FEBS Lett. 1997, 412(1):61-64). MJM tag T464        encodes the homologue of the GDP-mannose 3″,5″-epimerase of A.        thaliana, a key enzyme of the plant vitamin C pathway (Wolucka        et al., Proc. Natl. Acad. Sci. USA 2001, 98(26):14843-14848).        Consequently, increased ascorbate production might stimulate        alkaloid and phenylpropanoid biosynthesis as well, and plant        secondary metabolism in general.    -   C127 (SEQ ID NO: 38 and SEQ ID NO: 884): Sequence information        for an auxin-responsive GH3-like protein induced after 2 hour by        methyl jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx e-180)>    -   ref|NP-200262.1| auxin-responsive-like protein At5g54510 [A.        thaliana]    -   ref|NP_(—)194456.1| GH3 like protein At4g27260 [A. thaliana]    -   dbj|BAB92590.1| putative auxin-responsive GH3 [Oryza sativa        (japonica cultivar-group)]    -   gb|AAD32141.1|AF 123503_(—)1 Nt-gh3 deduced protein [Nicotiana        tabacum]    -   dbj|BAB63594.1| putative auxin-responsive GH3 protein [Oryza        sativa (japonica cultivar-group)]    -   ref|NP_(—)179101.1| putative auxin-regulated protein At2g14960.1        [A. thaliana]    -   pir∥S17433 auxin-regulated protein GH3 [Glycine max]    -   C175 (SEQ ID NO: 71 and SEQ ID NO: 885): Sequence information        for an auxin-responsive GH3-like protein induced after 2 hour by        methyl jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx)    -   ref|NP_(—)200262.1| auxin-responsive-like protein At5g54510 [A.        thaliana]    -   ref|NP_(—)1194456.1| GH3 like protein At4g27260 [A. thaliana]    -   dbj|BAB92590.1| putative auxin-responsive GH3 [Oryza sativa        (japonica cultivar-group)]    -   gb|AAD32141.1|AF123503_(—)1 Nt-gh3 deduced protein [Nicotiana        tabacum]    -   dbj|BAB63594.1| putative auxin-responsive GH3 protein [Oryza        sativa (japonica cultivar-group)]    -   ref|NP_(—)179101.1| putative auxin-regulated protein At2g14960.1        [A. thaliana]    -   pir∥S17433 auxin-regulated protein GH3 [Glycine max]        The Arabidopsis jasmonate (JA) response mutant jar1-1 is        defective in the gene JAR1, one of 19 closely related        Arabidopsis genes that are similar to the auxin-induced soybean        GH3 gene. Analysis of fold predictions for this protein family        suggested that JAR1 might belong to the acyl adenylate-forming        firefly luciferase superfamily. These enzymes activate the        carboxyl groups of a variety of substrates for their subsequent        biochemical modification. An ATP-PPi isotope exchange assay was        used to demonstrate adenylation activity in a glutathione        S-transferase-JAR1 fusion protein. Activity was specific for JA,        suggesting that covalent modification of JA is important for its        function. Six other Arabidopsis genes were specifically active        on indole-3-acetic acid (IAA), and one was active on both IAA        and salicylic acid. These findings suggest that the JAR1 gene        family is involved in multiple important plant signaling        pathways (Staswick et al., Plant Cell 2002, 14(6):1405-1415).        The MJM genes C127 and C175 cluster together with the        Arabidopsis genes At5g54510 and At4g27260, of which the protein        products display activity on IAA. They might participate in the        conversion of free, active IAA in inactive storage forms or        conjugates, and as such relieve the inhibitory effect of active        auxins on secondary metabolism, shown for instance for nicotine        production in tobacco cells (Imanishi et al., Plant Mol. Biol.        1998, 38(6):1101-1111) and terpenoid indole alkaloid production        in Catharanthus roseus cells (Gantet et al., Plant Cell        Physiol., 1998, 39(2):220-225).    -   T424b (SEQ ID NO: 570 and SEQ ID NO: 886): sequence information        for an auxin-induced reductase-like protein induced after 1 hour        by methyl jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx e-144)>    -   pir∥S16390 auxin-induced protein PCNT115 [Nicotiana tabacum]    -   ref∥NP_(—)564761.1| auxin-induced protein At1g60710 [A.        thaliana]    -   ref∥NP_(—)176268.1| auxin-induced protein At1g60690 (aldo/keto        reductase family) [A. thaliana]    -   pir∥T12582 auxin-induced protein [Helianthus annuus]    -   ref∥NP_(—)176267.1| auxin-induced protein At1 g60680.1 [A.        thaliana]    -   ref|NP_(—)172551.1| putative auxin-induced protein [A. thaliana]        This gene might encode a reductase protein capable of reducing        free, active IAA into the inactive form indole-ethanol (Brown &        Purves, J. Biol. Chem. 1976, 251(4):907-913). As such, it might        also be involved in the relieve of the inhibitory effect of        active auxins on secondary metabolism, shown for instance for        nicotine production in tobacco cells (Imanishi et al., Plant        Mol. Biol. 1998, 38(6):1101-1111) and terpenoid indole alkaloid        production in Catharanthus roseus cells (Gantet et al., Plant        Cell Physiol., 1998, 39(2):220-225).    -   T164 (SEQ ID NO: 446 or SEQ ID NO: 887): sequence information        for a probable glutathione S-transferase induced after 1 hour by        methyl jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx e-115)>    -   emb|CAA56790.1| auxin-regulated par glutathione S-transferase        protein STR246C [Nicotiana tabacum]    -   pir∥JQ1606 multiple stimulus glutathione S-transferase response        protein [Nicotiana plumbaginifolia]        This GST protein is induced also by auxins and might be involved        in the transport of IAA-conjugates, detoxification of secondary        metabolites or even in functions distinct from conventional GSTs        (as suggested by some characteristics of parA, Takahashi et al.,        Planta 1995, 196(1):111-117) such as an involvement in        transcriptional regulation.    -   MAP2 (SEQ ID NO: 284 and SEQ ID NO: 888): sequence information        for a protein with similarity to the putative protein At5g28830        [A. thaliana] induced after 6 hour by methyl jasmonate in        tobacco BY-2 cells.        Best Homologues found: (lowest blastx 3e-82)>    -   ref∥NP_(—)198235.1| putative protein At5g28830 [A. thaliana]        This protein contains a Ca-binding EF-hand motif. The EF-hands        can be divided into two classes: signaling proteins and        buffering/transport proteins. The first group is the largest and        includes the most well-known members of the family such as        calmodulin, troponin C and S100B. These proteins typically        undergo a calcium-dependent conformational change which opens a        target binding site. The latter group is represented by calcium        binding D9k and do not undergo calcium dependent conformational        changes. As calmodulins and Ca-molecules have been postulated to        be involved in jasmonate signaling cascades (Leon et al., J.        Exp. Bot. 2001, 52(354):1-9; Yang & Poovaiah, J. Biol. Chem.        2002, 277(47):45049-45058), possibly connected to the onset of        secondary metabolic pathways (Memelink et al., Trends Plant Sci.        2001, 6(5):212-219), they might be involved in nicotine alkaloid        or phenylpropanoid biosynthesis as well.

C1 (SEQ ID NO: 8 and SEQ ID NO: 889): Sequence information for a1,4-benzoquinone reductase-like induced after 12 hour by methyljasmonate in tobacco BY-2 cells.

Best Homologues found: (lowest blastx 5e-79)>

-   -   ref|NP_(—)200261.1| quinone reductase At5g54500.1 [A. thaliana]    -   emb|CAD31838.1| putative quinone oxidoreductase [Cicer        arietinum]    -   gb|AAD38143.1|AF 139496_(—)1 unknown [Prunus armeniaca]    -   ref|NP_(—)194457.1| quinone reductase family protein At4g27270.1        [A. thaliana]    -   gb|AAG53945.1|AF304462_(—)1 quinone-oxidoreductase QR2        [Triphysaria versicolor]    -   dbj|BAB92583.1| putative 1,4-benzoquinone reductase [Oryza        sativa (japonica cultivar-group)]        This reductase-like protein might be directly and actively        involved in the biosynthetic pathway of one of the nicotine        alkaloids.    -   T210 (SEQ ID NO: 466 and SEQ ID NO: 890): Sequence information        for a protein with similarity to the putative protein P0638D12        [Oryza sativa] induced after 6 hour by methyl jasmonate in        tobacco BY-2 cells.        Best Homologues found: (lowest blastx 5e-60)>    -   dbj|BAB55502.1| P0638D12.10 [Oryza sativa (japonica        cultivar-group)]    -   ref|NP_(—)565816.1| expressed protein At2g35680 [A. thaliana]    -   gb|AAK31276.1|AC079890_(—)12 unknown protein [Oryza sativa]    -   ref|NP-200472.1| putative protein At5g56610 [A. thaliana]        This protein contains a dual specificity protein phosphatase        motif. Ser/Thr and Tyr dual specificity phosphatases are a group        of enzymes (EC: 3.1.3.16) removing the serine/threonine or        tyrosine-bound phosphate group from a wide range of        phosphoproteins, including a number of enzymes which have been        phosphorylated under the action of a kinase (Fauman & Saper,        Trends Biochem. Sci. 1996, 21(11):413-417). As such, they might        be involved in the regulation of plant secondary metabolic        pathways.    -   C112 (SEQ ID NO: 22 and SEQ ID NO: 891): Sequence information        for a protein with similarity to the putative protein At3g11810        [A. thaliana] induced after 12 hour by methyl jasmonate in        tobacco BY-2 cells.        Best Homologues found: (lowest blastx 1e-10)    -   ref|NP_(—)187787.1| unknown protein At3g11810 [A. thaliana]    -   ref|NP_(—)178432.1| unknown protein; protein id: At2g03330.1 [A.        thaliana]        This protein contains a TonB motif. In Escherichia coli the TonB        protein interacts with outer membrane receptor proteins that        carry out high-affinity binding and energy-dependent uptake of        specific substrates into the periplasmic space. These substrates        are either poorly permeable through the porin channels or are        encountered at very low concentrations. In the absence of tonB        these receptors bind their substrates but do not carry out        active transport (Buchanan et al., Nat. Struct. Biol. 1999,        6(1):56-63.). As such, this protein might be involved in the        jasmonate-induced signaling cascades and thus in the regulation        of plant secondary metabolic pathways.    -   C454 (SEQ ID NO: 244 and SEQ ID NO: 892): Sequence information        for sequence a putative phosphatase 2C induced after 1 hour by        methyl jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx 4e-85)>    -   ref|NP_(—)180455.1| unknown protein At2g28890 [A. thaliana]    -   ref|NP_(—)563791.1| expressed protein At1g07630 [A. thaliana]    -   ref|NP_(—)195860.1| putative protein At5g02400 [A. thaliana]    -   gb|AAO65883.1| putative protein phosphatase 2C [Oryza sativa        (japonica cultivar-group)]    -   ref|NP_(—)187551.1| unknown protein At3g09400 [A. thaliana]    -   ref|NP_(—)182215.2| unknown protein; protein At2g46920 [A.        thaliana]    -   T172 (SEQ ID NO: 450 and SEQ ID NO: 893): Sequence information        for a protein phosphatase 2C induced after 4 hour by methyl        jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx e-104)>    -   ref|NP_(—)177421.1| protein phosphatase 2C (AtP2C-HA) At1g72770        [A. thaliana]    -   ref|NP_(—)173199.1| protein phosphatase 2C At1g17550 [A.        thaliana]    -   dbj|BAC05575.1| protein phosphatase 2C-like protein [Oryza        sativa (japonica cultivar-group)]    -   ref|NP-200515.1| protein phosphatase 2C, ABI2 At5g57050.1 [A.        thaliana]    -   ref|NP_(—)194338.1| protein phosphatase ABI1 At4g26080 [A.        thaliana]        Phosphatases have been postulated as important participants in        the jasmonate modulated signaling cascades (Leon et al., J. Exp.        Bot. 2001, 52(354):1-9) and as such represent potential powerful        master regulators of plant secondary metabolism. T172 shows most        homology to a group of 4 Arabidopsis PP2C phosphatases to which        also ABI1 and ABI2 belong, acting in a negative feedback        regulatory loop of the abscisic acid signalling pathway (Merlot        et al., Plant J. 2001, 25(3):295-303). C454 shows most homology        to a group of 5 Arabidopsis PP2C phosphatases to which also        POLTERGEIST belongs, encoding a PP2C that regulates CLAVATA        pathways controlling stem cell identity at Arabidopsis shoot and        flower meristems (Yu et al., Curr Biol. 2003, 13(3):179-188).        Both the T172 and C454 sequences are truncated clones and still        lack the N-terminal sequence. However, the clones available        cover the region corresponding to truncated mutant versions of        both ABI (Sheen, Proc. Natl. Acad. Sci. USA 1998, 95(3):975-980)        and Poltergeist phosphatases (Yu et al., Curr Biol. 2003,        13(3):179-188) that were shown to confer constitutive activity        and thus are very well suitable for metabolic engineering        purposes.    -   C477 (SEQ ID NO: 266 and SEQ ID NO: 894): Sequence information        for a putative zinc transporter induced after 4 hour by methyl        jasmonate in tobacco BY-2 cells.        Best Homologues found: (lowest blastx e-121)>    -   gb|AAL25646.1|AF197329_(—)1 zinc transporter [Eucalyptus        grandis]    -   ref|NP_(—)182203.1| putative zinc transporter At2g46800 [A.        thaliana]    -   gb|AAK91869.2| putative vacuolar metal-ion transport protein        MTP1 [Thlaspi goesingense]    -   gb|AAK91871.2| putative vacuolar metal-ion transport protein        MTP1t2 [Thlaspi goesingense]    -   ref|NP 191440.1 | zinc transporter-like protein At3g58810 [A.        thaliana]    -   gb|AAK69428.1|AF275750_(—)1 zinc transporter [Thlaspi        caerulescens]        Divalent cations are important both as cofactors for        biosynthetic enzymes and as active participants in elicitor        induced biosynthesis of plant secondary metabolites. For        instance calcium molecules and transporters/channels have been        shown to mediate fungal elicitor-induced beta-thujaplicin        biosynthesis in Cupressus lusitanica cell cultures (Zhao &        Sakai, J. Exp. Bot. 2003, 54(383):647-656). Zinc cations as well        might be involved, either as a cofactor in enzymes or zinc        finger proteins or as a secondary signal molecule, in        elicitor-mediated induction of tobacco secondary metabolism.    -   C331 (SEQ ID NO: 149 and SEQ ID NO: 895): Sequence information        for a protein with similarity to the putative protein At3g62270        [A. thaliana] induced after 12 hour by methyl jasmonate in        tobacco BY-2 cells.        Best Homologues found: (lowest blastx 7e-13)>    -   ref|NP_(—)191786.1| putative protein; protein At3g62270 [A.        thaliana]    -   ref|NP_(—)182238.2| putative anion exchange protein At2g47160        [A. thaliana]    -   ref|NP_(—)187296.2| unknown protein At3g06450 [A. thaliana]        This protein harbours a HCO3-transporter motif and might thus        function as an anion exchanger. Bicarbonate (HCO3-) transport        mechanisms are the principal regulators of the internal pH of        animal cells. As intracellular pH shifts have been shown to be        part of the signal mechanism leading to the elicitation of        benzophenanthridine alkaloids biosynthesis in cultured cells of        Eschscholtzia californica (Viehweger et al., Plant Cell 2002,        14(7):1509-1525; Roos et al., Plant Physiol. 1998,        118(2):349-364), this anion exchanger encoded by C331 might be        involved in regulating tobacco secondary metabolism.        8) Use of a Reporter Plant Cell Line as a Tool for Functional        Analysis to Accelerate the Identification of Genes with a Role        in Secondary Metabolism

The PMT gene encodes the enzyme putrescine N-methyltransferase,catalysing the first committed step in the production of nicotinicalkaloids. Transcripts of Nicotiana sp. PMT genes are reported to be upregulated by methyl jasmonate. When the flanking regions of Nicotianasylvestris PMT genes were fused to the β-glucuronidase reporter gene andintroduced into N. sylvestris, the reporter transgenes were found to beinducible by methyl jasmonate treatment (Shoji et al., Plant CellPhysiol. 2000, 41(7):831-839). We have applied this knowledge andconstructed a new reporter construct, called pHGWFS7-ppmt2, harbouring aEGFP-GUS fusion reporter gene (in Gateway® vector pHGWFS7; Karimi etal., Trends Plant Sci. 2002, 7(5):193-195), driven by the NsPMT2promoter. To this end, primers were designed for the Adapter attB PCRprotocol (InVitroGen) to amplify the NsPMT2 5′flanking region coveringnucleotides −1713 to +3 (Table 3).

The pHGWFS7-ppmt2 construct was subsequently introduced in the ternaryAgrobacterium tumefaciens transformation system,LBA4404.pBBR1-MCS-5.virGN54D (van der Fits et al., Plant Mol. Biol.2000, 43(4):495-502), allowing efficient transformation of tobacco BY-2cell cultures. Different independent transgenic lines were establishedand the jasmonate inducibility of the promoter in these transgenic BY-2cells was confirmed (Table 4).

These transgenic reporter cell lines are used as a tool to identifypotential master regulatory genes of plant secondary metabolism (andspeed up this process). Overexpression of a single gene most often doesnot affect significantly the final production levels of the targetmetabolite(s). Therefore, when accumulation levels are employed as theonly criteria to evaluate the potential involvement of regulatory genesin plant secondary metabolism, one might easily miss eventuallypromising candidates.

To illustrate the potential of this approach, BY-2-pmt2 cell line 7 wasdouble transformed with the pK7WGD2-C330 construct, harbouring the MJMtag with SEQ ID No 148, an AP2-domain transcription factor encoding gene(also designated as C330 in this application), driven by theconstitutive p35S promoter. Expression analysis of the reporter proteinsdemonstrated clearly that overexpression of the C330 gene induces theNsPMT2 promoter, without the necessity to use elicitors like methyljasmonate (Table 5).

In a next step, we evaluated if there was a correlation between theGUS-activity in the BY-2 reporter cell line (line 7) and nicotinealkaloid accumulation. Table 6A shows a perfect correlation between GUSexpression and nicotine alkaloids (as measured for nicotine, anatabineand anabasine). Table 6B shows the nicotine alkaloid content of the BY-2reporter cell line (line 7) super-transformed with an expression vectorcomprising the C330 gene (SEQ ID NO: 148). Measurements in tables 6A and6B were carried out in the presence or absence of synthetic auxins.“−2,4 D” means in the absence of dichlorophenoxy-acetic acid. “NAA”means in the presence of alfa-naphtalene-acetic acid. “DW” means dryweight, “MeJA” is with the addition of the elicitor methyl jasmonate,“DMSO” means with the addition of dimethylsulfoxide instead of MeJA.

9) Functional Analysis in Hairy Roots of Hyoscyamus muticus

Sterilized leaves of H. muticus were infected with a recombinantAgrobacterium rhizogenes strain (LBA9402) transformed with an expressionvector comprising the C330 gene (SEQ ID NO: 148). As a negative controlwe compared the infection with the LBA9402 wild type strain. The hairyroots appeared in the infected sites approximately 3 weeks afterinfection. The different root clones were separated and they were grownon plates in B50 medium added with cefotaxim to kill the excess ofAgrobacteria. The hairy roots transformed with C330 (4 clones: A, B, Cand D) and the control LBA9402 (one clone) were accurately weighed andthe same amount was added into each of the flasks (50+3 mg) then 20 mlB50 medium was added. For each of the clones three flasks were prepared.After growing for 21 days (16 h light, 8 h dark, 21° C.), the roots werefiltered and lyophilized. The tropane alkaloid extraction and analysiswas performed by a modified method of Fliniaux et al. (1993) J.Chromatography 644: 193. For analysis the three flasks of each clonewere pooled together and 50 mg dry weight (DW) was withdrawn for anextraction. For the GC-MS analysis, the samples were evaporated todryness and 50 μl of CH₂Cl₂ was added. The injected volume was 3 μl. Thewhole sample set was analysed in exactly the same way, which makes itpossible to compare between the samples. In our analysis the hyoscyaminecontent was measured as the sum of hyoscyamine and its isomer littorine,because of the difficult separation of these isomers in analyticalsystems. We observed no significant changes in the growth patternbetween the transformed and untransformed roots. The contents ofhyoscyamine in the hairy roots after 21 d was calculated and it wasfound that the hyoscyamine content was on average 25-fold higher intransformed roots compared to control roots, varying from 12-fold (cloneC) to 62-fold (clone B). In addition to possessing extremely highhyoscyamine content, in the chromatogram of clone B also several (5-10)new peaks were found which are currently being identified.

Materials and Methods

Alkaloid Analysis

Nicotiana tabacum BY-2 cells were cultured in modified Linsmaier-Skoog(LS) medium (Linsmaier & Skoog, 1965), as described by Nagata & Kumagai(1999). First, the growth curve of BY-2 cell culture was determined(FIG. 2) and the late exponential phase was used in elicitationexperiments. Since the ability of high auxin concentration to inhibitthe biosynthesis of nicotine is well known (Hibi et al., 1994; Ishikawa,et al., 1994), the six-day-old culture was prior elicitation washed anddiluted 10-fold with fresh hormone free medium. After 12 hours, thecells were treated with methyl jasmonate (MeJA). MeJA (cis-form, DuchefaM0918) dissolved in dimethyl sulfoxide (DMSO) and was added to theculture medium at a final concentration of 50 μM. Same amount of DMSOalone served as a control. Samples for cDNA-AFLP analysis were taken at0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 20, 24,36, 48, and 98 hours after jasmonate addition or at 0, 2, 4, 6, 8, 10,12, 14, 16, 24, 36, 48, and 98 hours after DMSO addition, respectively.For alkaloid analysis, the samples were taken at 0, 12, 14, 24, 48 and98 hours. Three replicate shake flasks pooled together yielded the totalculture volume of 75 ml. After filtering (Miracloth) under vacuum thecells were lyophilized. Lyophilized cell samples were extracted forGC-MS analysis by a modified method described by Furuya et al. (1971).Cells were weighed and 25 μg of internal standard (5α-cholestan) wasadded. The samples were made alkaline with ammonia (10% (v/v), 1 ml) andwater (2 ml) was added. Alkaloids were extracted by vortexing with 2 mlof dicloromethane. After 30 min the samples were centrifuged (2000 rpm,10 min) and the lower organic layer was separated and transferred intoglass vials. The samples were concentrated to 50 μl and 3 μl aliquotswere injected to GC-MS. In some cases (for derivatization of free fattyacids and more polar compounds) the samples were silylated prior toGC-MS analysis. After evaporation to dryness, 25 μl of dichloromethanewas added and silylation was performed byN-methyl-N-(trimethylsilyl)-trifluoro-acetamide (Pierce, Rockford, USA)at 120° C. for 20 min.

Analysis of Polyamines

Approx. 200 mg FW cells were homogenised using a mortar and pestle with10 vol 4% (v/v) perchloric acid (PCA), and the homogenate left on icefor 60 min then centrifuged at 20 000 g for 30 min. The pellets werewashed twice by resuspending in PCA and centrifugation at 15 000 g for 5min. The washed pellets were resuspended in the original volume of PCA.Aliquots (0.3 ml) of the supernatants and resuspended pellets werehydrolysed by adding an equal volume of 12 N HCl at 110° C. overnight inorder to release PCA-soluble and -insoluble conjugates, respectively.Hydrolysed samples were taken to dryness and resuspended in 0.3 ml 4%PCA. Aliquots (0.2 ml) of the supernatants and of the hydrolysedsupernatants and pellets were derivatised with dansyl chloride (Sigma)after alkalinisation with 1.5 M Na₂CO₃ (1 h at 60° C.), and dansylatedamines extracted in toluene. Standard putrescine, methylputrescine,spermidine and spermine solutions (1 mM in 4% PCA) were subjected to thesame procedure. Samples were injected into a fixed 20-PI loop of an HPLC(Jasco) for loading onto a reverse-phase C18 column (Spherisorb S5 ODS2,5-μm particle size 4.6×250 mm. Phase Sepand eluted with a programmedacetonitrile-water 5-step gradient as follows: 60 to 70% acetonitrile in5.5. min, 70 to 80% in 1.5 min, 80 to 100% in 2 min, 100% for 2 min, 100to 70% in 2 min and 70 to 60% in 2 min, at a flow rate of 1.0 ml min⁻¹.Eluted peaks were detected by a spectrofluorometer (excitation 365 nm,emission 510 nm), and their retention times and areas recorded andintegrated by an attached computer using the Borwin 1.21.60 softwarepackage.

Analysis of Sesquiterpenes

The sesquiterpenoid alkaloids were detected by GC-MS. The extraction wasperformed as described in the section of alkaloid analysis. Thepreliminary identification is based on the MS fragmentation pattern.

Detection of Phenylpropanoids by TLC

Phenylpropanoids (coumarins and flavonoids) were extracted from elicitedBY-2 cells or form the culture filtrate as described by Sharan et al.(1998). The methanol solutions obtained were concentrated and evaluatedqualitatively by TLC using silica gel plates with fluorescent indicatorUV₂₅₄ (Polygram® SIL G/UV₂₅₄, Macherey-Nagel, Düren, Germany) developedwith ethylacetate:methanol:water (75:15:10). Spots were visualized underUV₂₆₀ after staining with AlCl₂ (by spraying with a 1% ethanolicsolution).

RNA Extraction and cDNA Synthesis

Total RNA was prepared by LiCl precipitation (Sambrook, 1989). Startingfrom 5 μg total RNA, first-strand cDNA was synthesized by reversetranscription with a biotinylated oligo-dT₂₅ primer (Genset, Paris,France) and Superscript II (Life Technologies, Gaithersburg, Md.).Second-strand synthesis was performed by strand displacement withEscherichia coli ligase (Life Technologies), DNA polymerase I (USB,Cleveland, Ohio) and RNAse-H (USB).

cDNA-AFLP Analysis

500 nanograms of double-stranded cDNA was used for AFLP analysis asdescribed (Vos et al., 1995; Bachem et al., 1996) with modifications.The restriction enzymes used were BstYI and MseI (Biolabs) and thedigestion was performed in two separate steps. After the firstrestriction digest with one of the enzymes, the 3′ end fragments werecollected on Dyna beads (Dynal, Oslo, Norway) by their biotinylatedtail, while the other fragments were washed away. After digestion withthe second enzyme, the released restriction fragments were collected andused as templates in the subsequent AFLP steps. The adapters used wereas follows: for BstYI, 5′-CTCGTAGACTGCGTAGT-3′ (SEQ ID NO:_) and5′-GATCACTACGCAGTCTAC-3′ (SEQ ID NO:_), and for MseI,5′-GACGATGAGTCCTGAG-3′ (SEQ ID NO:_) and 5′-TACTCAGGACTCAT-3′ (SEQ IDNO:_); the primers for BstYI and MseI were5′-GACTGCGTAGTGATC(T/C)N₁₋₂-3′ (SEQ ID NO:_) and5′-GATGAGTCCTGAGTAAN₁₋₂-3′ (SEQ ID NO:_), respectively. Forpreamplifications, an MseI primer without selective nucleotides wascombined with a BstYI primer containing either a T or a C as nucleotideat the 3′ extremity. PCR conditions were as described (Vos et al.,1995). The obtained amplification mixtures were diluted 600-fold and 5μl was used for selective amplifications using a ³²P-labeled BstYIprimer and the Amplitaq-Gold polymerase (Roche Diagnostics, Brussels,BE). Amplification products were separated on 5% polyacrylamide gelsusing the Sequigel system (Biorad). Dried gels were exposed to KodakBiomax films as well as scanned in a phospholmager (Amersham PharmaciaBiotech, Little Chalfont, UK).

Quantitative Measurements of the Expression Profiles and Data Analysis

Scanned gel images were quantitatively analyzed using the AFLPQuantarPro image analysis software (Keygene N. V., Wageningen, N L).This software was designed for accurate lane definition, fragmentdetection, and quantification of band intensities. All visible AFLPfragments were scored and individual band intensities in each lane weremeasured. The raw data obtained were first corrected for differences intotal lane intensities which may occur due to loading errors ordifferences in the efficiency of PCR amplification with a given primercombination for one or more time points. The correction factors werecalculated based on constant bands throughout the time course. For eachprimer combination, a minimum of 10 invariable bands were selected andthe intensity values were summed per lane. Each summed value was dividedby the maximal summed value to give the correction factors. Finally, allraw values generated by QuantarPro were divided by these correctionfactors. A coefficient of variation (CV) was calculated by dividing themaximum value across the time course by the minimum value. This CV wasused to establish a cut-off value and expression profiles with a CV lessthan 4.0 were considered to be constitutive throughout the time course.Although differential and constant bands can be discriminated by visualscoring, QuantarPro-mediated analysis is more sensitive and reliable. Assuch, transcript tags that had been identified as jasmonate-modulatedafter visual scoring were excluded from the final data set because theyhad a CV lower than our threshold level. Vice versa additionaljasmonate-modulated transcripts were identified that had been missed bythe visual scoring. Subsequently, each individual gene expressionprofile was variance-normalized by standard statistical approaches asused for microarray-derived data (Tavazoie et al., 1999). For eachtranscript, the mean expression value across the time course of theDMSO-treated samples was subtracted from each individual data pointafter which the obtained value was divided by the standard deviation.The Cluster and TreeView software (Eisen et al., 1998) was used foraverage linkage hierarchical clustering.

Characterization of AFLP Fragments.

Bands corresponding to differentially expressed transcripts were cut outfrom the gel and the DNA was eluted and reamplified under the sameconditions as for selective amplification. Sequence information wasobtained by direct sequencing of the reamplified PCR product with theselective BstYI primer or after cloning the fragments in pGEM-T easy(Promega, Madison, Wis.) and sequencing individual clones. The sequencesobtained were compared against nucleotide and protein sequences in thepublicly available databases by BLAST sequence alignments (Altschul etal., 1997).

Isolation of Full-Length cDNA Clones.

Two strategies were followed to obtain full-length cDNA clonescorresponding to the short sequence tags isolated in the cDNA-AFLPanalysis. In the first method the use of gene-specific primers, RT-PCR,5′- and 3′-RACE (InVitroGen Life Technologies) techniques were combinedto yield a full-length cDNA clone. For the second strategy a cDNAlibrary from elicitor treated BY-2 cells was generated in thepCMV-SPORT6 vector (Gateway™, InVitrogen Life Technologies) using amixture of samples taken at different time points after jasmonateelicitation. This library was screened by PCR or colony hybridizationusing gene-specific primers or probes respectively. TABLE 1 Sequenceswith homology to known gene Seq code SEQUENCE Annotation SEQ ID N° BAP1aTTATCTCGGCGGCGAATCTACCCCACTCTTCGAAGA envelope SEQ ID N° 1TAACGCTCATTTTGTTACCATACTCACCTCTCTGAA polyprotein likeCAAACACACAAATACACACGAACTCACAGTCCAAA proteinTAGCTAAAACAAAGGTTTTTGAATTGAAATTGAAG CTCAGATC BAP1bGATCCTCTGAGGCTATTATGCTTGCTGGATTAGCTT glutamate SEQ ID N° 2TCAAGAGAAAATGGCAAAATAAAACGAAAGCCCA decarboxylaseAGGCAAGCCCTGTGACAAGCCCAATATTGTCACTG GTGCCAATGTCCAGGTGTGGTTGGGGCAAATTCGCCGCCGAGATA BAP2 GATCCAGACCATGCACACAAACACAAGATAGAAG abscisic stress SEQ IDN° 3 AAGAGATAGCAGCAGCTGCTGCAGTTGGGGCAAAT ripening protein TCGCCGCCGAGATABAP4a CAGAGCATGCACACAAACACAAGATAGAGGAAGA abscisic stress SEQ ID N° 4GATAGCAGCTGCTGCTGCAGTTGCGTAGACGGCGT ripening proteinAGTGATCCAGAGCATGCACACAAACACAAGATAGA GGAAGAGATAGCAGCTGCTGCTGCAGTTTGGGGCAAATTCGCCGCCGAGATCAG BAP4b GAGAAGACCA AGAAGAAGCA AAGGAAGAAT AP2-domainDNA- SEQ ID N° 5 CTTTATAGGG GAATCCGACA GCGTCCATGG binding proteinGGAAAATTCG CCGCCGAGAT GAG BMAP1 AGGAGCTGAACACACACCAACACCAACACTAACAputative protein SEQ ID N° 6 GGAGCTCCGTGGAGCACTGGCTTATTCGATTGTCATAt1g52200 [A. TTTGGACCAAACTAATGCTACTACGACAGCATTTTTA thaliana]CCTTGTGTGACATGTGGACCGTCGGCTGCATA BMAP2aCTAGTTTGGAATATGAGTTCTCTGCTCTTCGAGAAG putative potassium SEQ ID N° 7CCACAGAATCTGGATTTACATATTTGCTTGGACATG transporter TGGACCGTCGGCTGCAT C1GGGGGAGAAG CGAAGGTCTA AATCTAACCA 1,4-benzoquinone SEQ ID N° 8 AATCCCCAAAATGGCTACCA AAGTTTACAT reductase-like CGTATACTAT TCAATGTATG GTCATGTGGAGAAACTAGCA GAAGAGATAA AGAAAGGGGC AGCTTCTGTT GAAGGAGTTG AAGCTAAATTGTGGCAAGTA CCTGAAACGC TGTCGGAAGA TGTGCTAGCA AAAATGAGTG CACCTCCAAAGAGTGATGTG GCTGTTATAA CACCTCAAGA GCTTGCTGAA GCAGATGGTA TCATTTTTGGATTCCCTACG AGATTCGGAA TGATGGCTGC TCAGTTTAAA GCATTCCTTG ATGCAACTGGAGGTCTATGG AGAACACAAC AACTAGCTGG CAAGGCTGCC GGCATATTCT ATAGCACTGGATCCCAAGGC GGTGGCCAAG AAACTACACC GTTGACTGCG ATAACTCAGC TTGTTCACCACGGGATGATC TTTGTACCTA TCGGATACAC ATTCGGTGCT GGTATGTTTG AAATGGAGAAAGTGAAAGGA GGAAGTCCAT ATGGGGCGGG AACATTTGCT GGGGATGGCT CGAGACAGCCATCCGATCTT GAATTGCAGC AGGCGTTTCA CCAAGGTAAA TACATTGCCG GTATTGCCAAGAAACTCAAG GGTGCAGCCT AATTTCTCTC CTGCAAAGAT AATCTTTGCA TTCACACATTTCTTATAAAA TTTGAAAAAA GTACAAAATT TATCTTTGTG ATTGTTGAAG TCTTTTTTTTTTCCTTTATT GGGTATGAAA TCTCATCTAT ATGTGTCTGA TTCACAGTAA TTGTGTGTGTCAAAAGTACC AAATTGTGTT TTAAAATGGT TGCAAATACA A C10 GATCCCAGAA TAGCGTTGAGATAGATGATC cystatin SEQ ID N° 9 TTGCACGTTT TGCTATCCAA GATTATAACAAAAAACAGAA TGCTCTTTTG GAGTTTGGAA AGGTTGTGAA TGTGAAACAA CAGGTAGTTGCTGGAACCAT ATACTATATA ACACTCGAGG CAATTGAGGG CGGAAAGAAG AAAGTATATGAAGCCAAGAT ATGGGTTA C101 GATCCAATCG TTGGAATTTT GACAAGGCAT chloridechannel SEQ ID N° 10 GACTTTATGC CAGAGGATAT AAAGGGACTG protein C1C-1TACCCACATT TGGTCCATCA CAAGTAGCAG AGAGAAGCTA GCTCTTCCAA CAGGCAATCGGGCAACCATT ATTTGGGGAG TGTTATACAC ACATTCCACA TTGAGCTCTG TACACAATCTTCCCAAATTT TCTCATTGAC AAAATTGAAT TTAGTAGTCT CAATTAGAGC AAAAATTCTCCCTTACTTTG AATTGTTGAA CTTTCTTGTT TTTGGTGGTT TA C102 TGACGATGAGTCCCGAGTAA ACAAAATTGC putative protein SEQ ID N° 11 CATCTCCATCACATCCTAGT GACACTAGTT At5g47690 [A. CACAGAGTTT GGCATCATGG ATGTCCAGACthaliana] AATTGTGTAG ACCGACTGAA ATATGTCTGT TTATGAACTA AACACAAACTAATGACTTTC CTACATGTGG CGCTAATTGA AGAGAAGAGA TCCAAATACC CGTTATGAAGGCATATCAAC ACTACTACCA ATGAGTGTAT GGAACTTATA GAGCATTTAT CATCCTCTTCATCTCAGTGG ACCTCCTTGG ATCACTACGC AGTCA C104 GATCCAAGTG ACACCACTAAGCAACAATGA methylcrotonoyl- SEQ ID N° 12 CTATAGAGTT GAAGTCAATGGTCTAAGCCT CoA carboxylase GAATGTCTGC TTGGCTGCTT ATTCCAAGGA TCAAATTGAGCATATTCACA TCTGGCAGGG CAACTGCCAG CATCACTTCA AACAAAGGAT GGGCCTTGAAATCTTTGATG ATGATGAAAC GATAGACAAG CCTGCTCGCA TGGCAACATC TTATCCTTCTGGCACAGTGG TTGCACCCAT GGCTGGTCTA CTGGTTTA C105 GATCCAAGAA GAGAAAATGTCTGGTGAAGA 40S ribosomal SEQ ID N° 13 GGCTGTTGTT GCTGAGACCC CAGCTCCCGCprotein S12 TGCCGCTCTT GGTGAGCCCA TGGATATCAT GACGGCATTG CAACTTGTCCTCAGGAAATC ACGGGCTCAT GGTGGGTTAG CTAAAGGCCT TCACGAGGGT GCAAAGGTCATCGAGAAGCA TGCTGCCCAA CTTTGTGTAT TGGCAGAGGA CTGCGACCAA CCAGACTATGTGAAATTGGT CAAAGCGCTA TGTGCTGATC ACAATGTTA C106GATCCAACCCAATAACACCTTCAAATGCCACATGG putative protein SEQ ID N° 14TCCAGCTGAATGTTTTTTGGACACTTTAGAGGGTTG At1g07080 [A.TGCAATTTGATGCCTGGCCAGATTTGAATGAACATTT thaliana]TCCTTTCATTTACTGTGTGGAAAGTTTGGTCTACCATAAGAATTATACCCAGTGGGAAACATGTTTTTGAAA AACTGAATTTGAAGGCAAAGCTTGTTA C107TTTGAACCCTGATAACAAAGCTGGGAGGATTACAA 3-deoxy-D-arabino- SEQ ID N° 15AATTTACCAGAATGGGAGCAGAGAACATGAGGGTT heptulosonate 7- A phoshate synthaseC108 AATTACAATA CTTATAGTTT CGATGGAAAG putative protein SEQ ID N° 16AAGAAGCTTG TGCTTTCTAC AACTAGCTGG At1g54320 [A. ATTGGCGGAA AGAATGATTTTCTTGGTATT thaliana] GCTTATCTTA CTGTAGGTGG ATTGTGTTTC TTTCTGGCCATGGCTTTCAC GATCGTGTAT CTAGTTAAGC CAAGGCAGCT TGGAGATCCA ACATACTTTGCGTGGAACCG GAACCCGGGA GGTAACTAGT ATGCAAATGA AGTCTTTTGG CTTGAGCGCTTTACCATCTA AGGTTGATGT TGACAAAGCT TGTGTCTTGT AGCAGCTATC TGTCTACAAGTTCTTTTTTT TTGAAATGTT CTGCATATAC TTTTAAACTC AATTTGCTAG GAAAACAATGATATGTAATG AAGTATTTTC CCTTTGTTAA GTGTTTATCC AAAATTATGT ATGTACAATGGAAGTAATTG CTTAAAGGAC TTGAATGATG CC C109GATCCAAGTGCGGACGGTGTTCACCATGTAAACCG putative protein SEQ ID N° 17GTTCGAGTCTCCGTTCAACCTGGTTTTACCTCTACT At3g22820 [A.TTAGAGTACTACCCTGAAGCTTTGGAGGTGCAAGTG thaliana] TCGCAACAAACTCTTCATGCCTTAC11 GATCCCACAA TATTCATATG TAACTCCGAC putative protein SEQ ID N° 18GAAATGGAAT TTGGTGACGT GGTTTCAGCC At2g23690 [A. ATAAGTGCCG ACGAGGAGCTTCAACCGGGT thaliana] CAACTTTACT TTGCGTTGCC TTTGAGCAAT CTGAAACGTAGGCTTCAGGC TGAGGAAATG GCAGCATTAG CCGTTA C110bTAAGGCTCTCTTCAGAAGCTACGTGTGCCGATGATC CTR1-like protein SEQ ID N° 19CCAATTTCTTGGATC kinase C110c TAAGGTGGTTGAGTTTGAACTTCCACGGCAACAATputative protein SEQ ID N° 20 GTATAGTCTACTTGGATC At2g46260 [A. thaliana]C111 GATCCAAGAA TAAAGGGTCT ATTTTTTCAC putative protein SEQ ID N° 21CAAACAACAT TCAGTATTGG CTTGTCCAAA At2g46750 [A. GTAAAAAACT TTATACAAGATGTGCAAAAA thaliana] CTTGTGGTTT TACAGCCTAA GGCATTATGT GGTTTAGACCTATACAGTGG AATCCTAATG AGGTATGTCA CAGCTTCAAA TGCTTACTTG GGACATCAAGAAGATGCAGT GGATTTTGAT ATAACATATT ATAGAAGCAA AAATCCATTG ACTCCTAGGTTATATGAAGA TATTCTTGAA GAAATAGAGC AAATGGCGAT GTTCAAATAT GGAGCAGAACCTCACTGGGG GAAGAATCGT AATGTGGCAT TCATTGATGT GATTA C112TAGCGGATAACAATTTCACACAGGAAACAGCTATG myosin-heavy-chain SEQ ID N° 22ACCATTAGGCCTATTTAGGTGACACTATAGAACAA kinase-like proteinGTTTGTACAAAAAAGCAGGCTGGTACCGGTCCGGAATTCCCGGGATTTCTTCTTCATCATCGATTTTTAGCTCAAATGTCGTCTGCTTCTACAGAAAATCGTAGCCTT TGGACAGAGATCCGAGAATCAATAAGGAGCATATTGAAAGCTAATTGTGGCCATTTTCATACTCTTTTTATCCTCTTCCTCTTGCCTATCTTTTTCTCTCTCGTCGTTTATCCTTCTTTCCACCTTGCCCTCTTTCATCCGGACTATGATTTCACTCAACCAGTTCAATTTTCACACTTTTTAAGTTCACACTTCGAAATTATTGTACCCATAGTATTTACTCTGTTTCTGGTCCTCCTTTTCCTCTGTGCTGTAGCCACGATACATACAGCGCGCTTCATGTATCCTA TGGTAGACCTATCAACCTCGTTTCCTCTATTAAATCTATCAGAAATTCCTTCTTCCCCCTTCTCTCCACCTTTATCGTTTCGCATACCATTTTCATTTCAATCGCTCTCGTTTTCTCCCTTGTCTTGGTTTTTTTAGTCCAGGTTCTTCAAACTCTTGGATTAATTGAACTAAAATACGACTCGAATCATTTCTTGTTTTTGGTTATTCCCGCGTTGATTGTGCTCGTGCCAGTTTTGATATGGTTGCAGGTTAATTGGTCATTAGCTTATGTGATAGCAGTAGTCGAATC GAAATGGGGTTTCGAAACACTAAGGAGAAGTGCCTATTTGGTAAAGGGGAAGAGATCGGTAGCTTTGTCG ATGATGCTGTTATACGGGCTTTTGATGGGAATAATGGTGGTTTTAGGTGCCATGTATTTAGTCATTATGGAT GCAGCGAAGGGTCGTCAATGGAGAAGTTCAGGGGTAATATTACAGACTGCTATGAGTTTCAATAACTAGCTATCTCATGATGAGTCAATTTCTTGTGGGGAATGTTGTTTTATATCTGCGTTGCAACGACTTGAATGGTGAAAAATTGCCCTTGGAAATCGAGCATCTTCTTCTTCATCAATCTTTAGCTAATGATCACCCACCTCCAATGTTGT CAGCTTCAACGAAAAATCTTAGCCTATGGACAGAGGTCGTAGAATCAGCAATGAGCATATTCAAAGCCAATTCTGGCCATTTCCATGCTCTTTCAATCCTCTTCCTCTTGCCTATCTCTTTCTTTCTCGTCGTGTATCCTTCTTTCCACCTTGCTCTCTTTCATCCGAACTATGATTTCATCAGTTTCGCTCAACGCCATCTTTTCCTTTCAAATTTCGAAATTATTGTACCAACATCGTACTCTTTGTTTTTGGTCCTCCTTTTCCTATGCGCCGTAGCCACAACTACATATAGCGCGGTTCATGCATCCTATAGTAGACCTATAAACCTCGTTTTGTCGATAAAATCGATCAGAAAGTCTTTGTTCCCCCTTCTCTCCACCTTACTCGTTTCGCATACCATTTTCATTTCAATCACTCTTGTTTTCACCCTAGTCTTGACTATTTTAGTTTCAAATTCTTCAACCTCTCGGACTAATTGAAATCAAATACGATTCGGATCACTTCTTGCTTTTGGCTATTCCTGCTTTGGTAGTGCTCGTGCCAGTTCTGCTATGGCTACATGTTAACTGGTCATTAGC TTATGTGATAGCAGTAATTGAATCGAAATGGGGTTACGAAACATTGAGGAGAAGTTCCTATTTTGGTGAAGGGGCAAAGATGGGTAGCTTTTGGGATATATTTTATATTACGGGCTTTCAATGGGAATAATGATGGTTTGTGGTTCAATGTTTTTTGTCATTATGGGTGTAGCGAAGGG TAATAAGTGGAGGAGCTTGGACGTGATACTGCAGACTGCGCTAGTTTCAGTGATGGGATATCTGACGATG AATCAATATCTTGTGGCGAACGTGGTTTTGTATATGAAATGCAAGGATTTGAGCGTTGAAAAATTGCAGTC GGAAACTGGAGGCGAGTACGTTCCCCTGCCCTTGGATGAGAAGAATCAAGCTATTTGAATAATLTGTAAACAGTGAATCTGGTAGGCTATTTGTGTAACACTTCCTTTGATTAATGCTTTGTACGAGTATAATGTTTGGTTGTCTTTGTAGAAAGTTAAACGTGTGTGCTAAATGTTCTGCTCGTCTTTCCTGTTTGTTGAATATTTGAATAAAAAC C114GATCCAAAAGTATGCACGATCTTTCAAGCCATGAT diacylglycerol SEQ ID N° 23AATCATGATGGTGATGATGGGGATAGTGGTGAAGA kinaseGGATTCGGTTGTGGAAGAGCAGAGGAAGTTTGGGGCAGCAGACACATTTCAAAATTTCCTGATGAAGTTTGACATTTCTCATCTCAGTTGATTCTGTTATCTCTCGTCGTTCAAAATTTTGCTTTCTACTACAACCTCCATATTA C116aGATCCAAGATGGGAAGAGGATTTTACTTTTTGTGTTTG putative calcium SEQ ID N° 24GAGGAGCCTCCTGTGAATGATAGGCTGCATTTGGA lipid binding proteinAGTTCTCAGCACCTCAATGAGGATTGGCCTATTGCATCCTAAGGAGGTATTGGGTTATATTGATATAAGCCT TTCCGATGTTGTTA C116bGATCCAAGCCAAAGTTGGAACAAGGCTCTCAAACT subtilisin-like serine SEQ ID N° 25ATCACAAATAGCATCGACCAAGAAGGAGTTTGAAG proteaseCGCTTGGTGTTTTATTTTCTAGTCATTATTATATGAGTACAATGACAATATGAACAATAAAGTATTGTATAG TATGGTTTTATATTA C117aTAATGCCTAAAGTGTCATCTTATAATGCTTTGGATC homeodomain SEQ ID N° 26ACTTGTCATTATTTTCTTCAACTTACACTCAGTTATT protein GGATC C117cGATCCAAGTTGTGGCGGCAAGTTGGCGAGTCGTTT putative DNA- SEQ ID N° 27 A bindingprotein C117d GATCCAAGTTCTTTGAGCAGGGTCTAAATAATCTAT putative protein SEQID N° 28 CATTGGAGGAAAAGGCCAACCGGAAGGATTCGGC P0410E01 [Oryza GATATTAsativa] C118c GATCCAAGCAGATATTGAGATGAAATGTTTTCAGT putative eukaryoticSEQ ID N° 29 TTGATCGGTGTTATTCACATTA translation initiation factor 2alpha C118d GATCCAACGAAAAACAAGAAGCGCCCTGATTTTGT putative cellulose SEQID N° 30 GAAGGATCGACGTTGGATTA synthase C119GATCCACAATCTCTTGGAATGGATTGCAGTGACAC putative ABC SEQ ID N° 31TATTCTCGGAAATCCAACAGAATGTGAACTATACA transporterAAGCCCTTGGAAGTACAATTCACAACAACTTGTCT GGCTTTAGCGAGAATACTGTTAGAAAATCCATCTATACTGATATCGTAGTGTTTA C12 GATCCCAACT ATTGACACCA TACCCCGGAA aldehydeoxidase SEQ ID N° 32 TTTCAACGTT CATTTGGTAA ACAGCGGACA TCATGAAAAACGGGTTCTCT CTTCCAAAGC ATCTGGTGAA CCGCCACTGC TATTGGCAGC TTCAGTCCATTGTGCAACAA GAGCAGCCGT TA C120 TAACGAAGTTGCCAAGGGTTTTGGTGGATC 40Sribosomal SEQ ID N° 33 protein S2 C121GATCCACACCCACATGTGCTACTCCAACTTCAACG methionine synthase SEQ ID N° 34ACATTATCCACTCCATCATAGACATGGATGCTGATG TGATCACCATTGAGAACTCACGTTCTGATGAGAAACTCCTCTCAGTTTTCAGGGAGGGAGTGAAGTACGG AGCTGGCATTGGTCCCGGTGTCTATGACATCCACTCTCCAAGAATACCATCCACAGAGGAGCATAGCTGAT GAGGTTA C124bGATCCACTAATTATTGGAACACAAGTAAAGCCACG membrane protein SEQ ID N° 35CGATGAATTGTTTTGGTTTGGGAAACCGAAGATAC Mlo4 TATTACGGTTA C125TTACGTTTCTGTTTCTGAGTCTGGTTCTCAGGACT methionine synthase SEQ ID N° 36CATCGTCAAGAACTCACGTTCTGATCAGAAACTCCT CTCAGTTTTCAGGGAGGGAGTGAAGTACCGGAGCTGGCATTGGTCCCGGTGTCTATGACATCCACTCTCCA AAGAATACCATNCACAGAGGAGATAG C126TAAGCCCGCACGAGAAGGTGATTTGGAGGGAATTC cathepsin B-like SEQ ID N° 37CACTTCTAACTCATCCTAAACTTTCGGAGCTACCAA cysteine proteinaseAAGAGTTTGATGCACGAAAAGCTTGGCCTCAATGT AGCACTATCGGAAGAATTCTGGATCAGGGACATTGCGGTTCTTGTTGGGCTTTTGGTGCTGTTGAATCGTTGTCTGATCGTTTCTGTATCCATCACAACTTGAATATCTCTCTGTCTGTAAATGATCTGCTAGCATGCTGTGG CTTTTTATGTGGATC C127AGCAGGCTGGTACCGGTCCGGAATTCCCGGGATTG auxin-responsive SEQ ID N° 38TGTGTACAAATTACTAATATAGTTTCTTCACAATTA GH3-like proteinTGGAAAGAAACGTAGCTAATGAGGCACCAAAGGC CACAATAATGGCGGAGGATTACAAGAAGGATCTTGAGTTCATTGAAGAGGTGACTAGCAATGTTGATGAG GTCCAAATGAGAGTTCTTGCTGAAATCCTCTCCCAGAATGCACATGTTGAGTACTTGCAACGCCATAATCTC AATGGCAGCACTGATAGAGAGACATTCAAGAAAGTCGTACCTGTCATCACTTATGAAGATATTCAGCCTGATATCAAACGCATAGCCTATGGTGATAAATCTCCTATCCTCTGCTCCCAACCCATCTCTGAATTATTGTCAAG TTCTGGCACCTCTGGAGGGGAGAGCAAATTGATACCAACAACAGAGCCAGAGATTGGGAAGAGACTACA GCTTCACAAACTTGTGATGTCTGTGTTGAGCCAAGTGGCTCCAGATTCTGGAAAGGGCAAAGGAATGTATT TCATGTTCATAAGCCCTGAACAAAAGACCCCAGGAGGATTATAGCTCGCTTTCTTACAACTAGTTATTTACAATAGTCCTTACTLTCAACTACAGTCGTCTTCATAACCCCCATTGTAACTACACTAGTCCAACTGCAGCCATTCTCTGCCCAGACTCTTACCAAAGCATGTATTTCCCAAATGCTTTGTGGCCTCTGCCAAAACAACCAAGTCCTCCGTGTTGGCTCCTTTTTTGCGACCAGCTTCGTTCGTGCCATCCGATTCCTGGAGAAGCACTGGTCTCTACTT TGTAACGATATCCGAAGCGGAACCATTAACACTCAAATAACTGATCCTTTAGTGAGAGAGGCAGTGATGG AAGTCCTCAAACCTGACCCAACATTAGCTGATTTCTTGAGGTTGAATGCACCAAAGATTCATGGCAAGGG ATCATCACTAGGTTATGGCGTAATACCAAGTATGTGGATGTTTATTGTGACTGGATCCATGTCACAATATATACCGATACTTGATTATTACAGCAACAATCTCCCTCTTATCAGTACTCTGTATGCTTCCTCGGAAAGCCACTTTGGAATCAACTTGAACCCTTTTTGTAAGCCCAGTGATGTCTCTTACACCCTTATTCCCACCATGTGCTATTTTGAGTTCTTACCGTATCGCGGAAACAGTGGAGTCAT TGATTCTATATCCATGCCCAAGTCGCTTAATGAGAAAGAACAACAACAATTGGTTGATTTGGCTGATGTCA AGATTGGCCAGGAGTACGAGCTTGTTGTTACCACATATTCTGGACTCTACAGATATAGAGTCGGTGATGTG CTTCAGGTTGCTGGATACAAGAACAACGCGCCTCGATTCAACTTCCTATGCCGGGAAAATGTAGTCTTGAGTATTGGTGCTGACTTCACTAATGAAGTTTGAGCTACAAAACGCAGTGAAAAATGCAGTGGGCAATCTGGTGC CATTTGATTCTCAGGTAACCGAGTACACCAGCTATGTCGATATTACCACCATTCCAAGCCACTATGTCATATTCTGGGAACTGAATGCGAATGACTCTACCCTGGTTCCTCCTTTCAGTCTTTGAAGATTGTTGCCTCACAATTGAAGAATCTCTTAACTACTTCTACCGCGAGGGCCGT GCGTCTAATGAATCCATCGGGCCTCTAGAAATTAGGGTGTTGGAAATTGGAACTTTTGACAAGCTCATGG ACTACTGCATGAGCTTAGGTGCTTCCATGAACCAATACAAGACGCCCCGCTGTTTGAAATATGCACCCCTT ATTGAGCTATTGAACTCTAGGGTCGTGTCCAGCTACTTCAGTCCCATGTGTCCAAAATGGGTTCCTGGCTAC AAGAAATGGGACGGCAACAATTAAATGTCAAACTTCCGATTTCCCTGCTTGTACCTTCATTCACTATCCAG AAAAAAGACAACCATTTGTGGATTATTTAGTCAATCGTCATCCTAGCTAAGTTAGTCTTTCGTGAACATGGTATGGATTTGTATTTGTCACAAATAAAATATGGCAC TTTTTATTTTCAAAAAAAAAAAAAAA C129bGATCCACCAAGAAGAAAGCATATGGTGTATCTTGG actin related protein SEQ ID N° 39AGGTGCGGTTCTGGCAGGAATTATGAAGGATGCCC CTGAGTTTTTGGATCAATAGACAAGATTATTTAGAAGAGGGAGTTGCTTGCTTA C130 GATCCACACAAAGCAGCTAGAGTTTGGTTAGGCAC putative AP2SEQ ID N° 40 ATTTGATACAGCTGAAGCTGCCGCTAGAGCTTATG domain containingATGAAGCTGCTCTTCGATTCAGAGGAAACAGAGCT proteinAAGCTCAATTTCCCCGAAAATGTCCGCTTATTACCA CAACAACAACAACAATATCAACCCACAACAAGATCAGCC ATTCCAGCT CCTCAGCAGCTTCACAATTCCCATTA C131 TAATCCTTTG AGCGAACGTATAGTGGAGCT H+-transporting SEQ ID N° 41 TCAATATGAT ATACGACTGA AATTAGGAGCATP synthase CTTGATGCCT AAGGAGAGTG CCCAAAAAGT protein 6 TTTGGAAGCTTCCGAAGCTT TACATGGGGA AAGCAACAAT ATCGCCTTTC TTGAATACCT TTTGGAAGATTTGCAGCAAA ACGGAGTAGG GGGAGAAGCC TATAAAGATG CGGTGGATC C133GATCCACAAGTGATCCATCATTCTAAAGGCCATAC putative protein SEQ ID N° 42CATACCAAAATTAGATGATAGCAGCCTTGAAATAA At4g24380 [A.TGCTTGGGTTTATTGAAAAAATTCAAAACCTGTGA thaliana] GACTGCACGAGGAATTA C134GATCCACACCCCATATTGTTCACGCTCACCTCACTG putative protein SEQ ID N° 43ACGAGCCACCATTA PH1760 [P. horikoshii] C135aGATCCAGTATTTGATAGTAGAAATGTCGCGTAAGG high affinity sulfate SEQ ID N° 44AATTTCCAAAAACTATATTCTTCGAATTTTCTGTCC transporterCTGAGGTTTTCATTGAGTAACTTGATTCTTGCTCTCTTGCAGCTGTTACTGATATAGATACAAGTGGAATTC ATTCCTTAGAGGATTTGTTTA C135bGATCCAGGAAGTTGGAAGATATTGGTAATCAGTAC NBS-LRR type SEQ ID N° 45TTTGATGAGTTACTATCAAGGTTTTGCTTCCTAGAT resistance proteinGTGGTACAAGCTTTTGATGGAGAAATATTGGCTTGTAAGTTACACAATCTTGTGCATGATCTTGCACAGTCA GTGGCAGGTTCTGAATGTTTA C136GATCCAGGTA GTTTCAAGAC ATTTGATCTT Peroxidase SEQ ID N° 46 AGCTATTACAAGCTTCTGCTCAAAAGGAGA GGCCTATTCC AATCTGATGC AGCTTTA C137 GATCCAGGAAAGGAGCATTG AGAAGGTGTA Tobacco SEQ ID N° 47 AAATGGATATTGTGATATCTCAAAGGCCCC retrotransposon TCAGGTATGG CACTTTGTTT A Ttol C140TGACTGCGTAGTGATCCAGGCAGCACTGGCTGAGT glutaredoxin SEQ ID N° 48GGACTGGTCAGCGCACTGTGCCAAACGTCTTGATT GGCGGGAAGCACATTGGTGGCTGCGACGCCACAACTGCGTTGCACAGGGAAGGGAAGCTTGTTCCTCTGC TAACTGAGGCTGGAGCACTTGCTAAATCTTCTTCTGCTTAGAGGATCAAATAGTCAGTTGTTTTTTTTAGTA AATCAGTCTCGTGAACTTA C143CCTGACTCGGTTTCGTGATGCTAGCTCGTGAACCAAT putative chorismate SEQ ID N° 49CATTTCCTCGAACCGACCGGCCATTCAAAACAAGCA mutase/prephenateTCGTATTCGCACATCACGAAGGAACAAGCGTCCTT dehydrataseTTCAAAGTTCTATCGGCGTTTGCATTCAGAAACATA AGCTTA C144TAAGCAAAAGAAACTCCAAGTATAGCACCCACAGA caudal protein SEQ ID N° 50TGAGAAATGGGGCTCACCAAAACAATCCTCTCAAA CCAACAATACCTCAACCGTCGAGTGGCGTCTCAACACCTGGATC C145 GATCCAGGTG GCTTGACCAT TCTCCTGCCG leucoanthocyanidin SEQID N° 51 GACCAGGACG TCGCCGGCCTTCAGGTCCGC dioxygenase-like CGCAACCGCGATTTGGATCAC TGTAAAGCCA protein GCTTCTCATGCTTTCATTTGT CAATATAGGTGATCAGATTC AGGTATTA C147b TAATCAGGGGCAATGTTGCTGTGCTGGATC aldehyde SEQ IDN° 52 dehydrogenase C147c GATCCCCTCATCAAGGCCAATGACACCATTA ribosomalprotein SEQ ID N° 53 S4 C149 TGTGATCACAATTGAGAACTCACGTTCCAATGAGAmethionine synthase SEQ ID N° 54 AGCTCCTCTCAGTTTTCAGGGAGGGAGTGAAGTATGGTGCTGGAATTGGCCCTGGTGTCTACGACATCCA CTCTCCAAGAATACCATCAACCGAAGAAATTGCTGATAGAGTGAACAAGATGCTTGCTGTTCTTGACACC AACATCTTGTGGGTCAACCCTGACTGTGGTCTCAAGACCCGCGAGT C150 CCAGGTGGTTTACATACAAAACATATTCCAGCTGTC putative SEQ ID N°55 AGCAGTTTACAGGAGCATATAGTTCGGAATCCAAC aminotransferase-ACAGGCAAGATATAATAGTACAGAGGCATCTTTGC like proteinAAAATGATATTCCAGCAACTGATAATAGAGGGTTT AGGGGTCATGATATGTTGGCACCCTTCACTGCTGGGTGGCAAAGTACTGATGTGGATC C157 GATCCACAGA AATAGGAGGA AAAAATGAGA putativeprotein SEQ ID N° 56 AAATATCTTC TGCTTAGAGTGTTGTCAAAG At1g31040 [A.CTTTTGCCCT CACTGCCTTC CTTCTCATCA thaliana] TTTTTGTCCTCTTCTCTTGGTCTCTCTCTG TATAATTATG TAGTAGATAA AAGC C159 GATCCACAAC AATGCATCACAACTATGGAT putative protein 103 SEQ ID N° 57 TCCAATTATT CGATTTTTTCTTTCCCTCGC [Nicotiana tabacum AATATGATCT A chloroplast] C15bGATCCCACAAATGGAGGGTATATTTGACAACTATTT Chaperonin SEQ ID N° 58CCGTGAAGCGTCAGATTGTTA C16 TTGATTCGGATTGAGGGAGTGAATACTAAAGAAGA putativeribosomal SEQ ID N° 59 AGTGGATTGGTACTTAGGAAAGCGTCTGGCTTATA proteinTTTACAAGGCCAAAACAAAGAAGAATAATTCAGCA TTATCGTTGTATTTGGGGTAAAGTTTGTAGGCCACATGGTAACAGTGGTGTTGTTA C160 GATCCAGGTCTGGTTTTATGATATTGAAATGAAGGAsubtilisin-like serine SEQ ID N° 60 TTACGTGAATTTTCTTTGCGCCATTGGTTATGACCCproteinase CAAAAGGATTTCACCGTTCGTGAAAGATACTTCTTCGTGAATTTGCAGTGAAAAGAGTTTTAGTTAGTCCAGGGGATTTGAATTATCCGTCGTTCTCAGTTGTTTTTA GCAGTGAGAGTGTGGTAAATAC C162aGATCCAGCACCATGAATACATGGGCTTCGAGAACC putative protein SEQ ID N° 61GCAAATATGATCCTTA At2g25740 [A. thaliana] C162bGATCCACAGAGTATTTGCAGCCAAGAGTCGTAGAGA putative protein SEQ ID N° 62ACGGATCAGTGAACGCCTTA At5g37800 [A. thaliana] C163 GATCCAGACC CAACAAAGATGAATGTGCCT glycosylated gag SEQ ID N° 63 TTTGTCGAGA AAAAGGGCACTGGAAGAAAAprotein GACTGTCCGA AGTTGAAGAA TAAGGCCAAA TATAATAATGGAAAGGCCAT TATGGATTGAAATGTAGCTG ATTGTGATGA TTCAGACTTTCTCATTA C165 TGCATCCAAC GCGTTGGGAGCTCTCCCATA putative ligand- SEQ ID N° 64 TGGTCGACCT GCAGGCGGCCGCGAATTCAC gated ion channel TAGTGATTAG CGGATAACAA TTTCACACAG proteinGAAACAGCTA TGACCATTAG GCCTATTTAG GTGACACTAT AGAACAAGTT TGTACAAAAAAGCAGGCTGG TACCGGTCCG GAATTCCCGG GATTTTTTAT TCTTTCAGGT TTAGTTTCTCAACAATGTTT TTGGCACACA GAGAAAACAC AATGAGCACC TTGGGACGCT TAGTGCTCATCTTCTGGCTC TTTGTCGTTC TAATTATCAA TTCGAGCTAT ACAGCTAGCT TGACATCTATCCTGACGGTG CAGCAGCTGT CTTCAGGAAT TCAAGGAATT GACAGTTTAA TTTCAAGTAGTGATCAAATA GGAGTCCAGG ATGGGTCATT TGCATATAAT TACCTCATTG AAGAGCTAGGTGTTTCAGAA TCACGGCTTC GTATATTGAA AACTGAAGAT GAATATGTCA GTGCCCTCGAGAAAGGTCCA CATGGTGGTG GTGTTGCTGG CATTGTCGAC GAGCTCCCTT ATGTTGAGCTCTTCTTATCC AACAACAAAT GCATATTCAG GACAGTAGGG CAGGAGTTCA TAAAGGGCGGATGGGGCTTT GCATTTCAAA GGGACTCTCC GCTGGCTGTT GATCTGTCAA CTGCAATTCTTCAACGGTCA GAGAACGGTG AACTCCAAAG GATTCATGAC AAATGGCTAA CGAACAACGGATGCTCTTCA CAAAACAACC AAGCTGATGA TACTCAGCTT TCTCTCAAGA GCTTCTGGGGCCTATTTCTC ATATGCGCCA TTGCTTGCGT CCTTGCTCTT ATAGTGTTTT TCTGCAGGGTATACTGTCAA TTCCGGAGGT ATCACCCCGA GCCAGAGGAG CCGGAGATCA GTGAACCTGAATCTGCACGA CCTAGTAGGC GTACCCTCCG CTCTGTTAGT TTTAAGGACT TGATAGACTTTGTCGATAGA AGAGAAAGTG AAATTAAGGA AATACTCAAG CGTAAGAGTA GTGATAACAAGAGACATCAA ACTCAGAACT CAGATGGGCA GCCGAGCTCG CCTGTTTGAA ACAAAAATTTGTGGTCGGGT TTGTTAGCTC TTGCTCAATA CACTTATGGT TGATATGTAA ATGATGCATGTACAATTTTA TTGTTGAATT ACCTCATTTC ACAC C166GATCCATCGTCTTGCTCGCTATTACAAGAAAACAA 40s ribosomal SEQ ID N° 65AGAAGCTCCCACCTGTCTGGAAATACGAATCAACC protein S13ACTGCTAGCACGCTTGTGGCTTAGGGTGAGCCTTG GGCTGGAGTAGTTTTGGCTGATGGCAATATGTTGTTTTCTCGTGTCATGAATTACTTTGTTACTCAGGACTCATCGAAGCTCCACTCGTTCTGCTCGGTGACCTCGTCG TCGTTGTCGTGTTTA C169 GATCCATGCAGCAATCAAGC GCTTTGAAGT Glutathione S- SEQ ID N° 66 TGACATGAAT CAATTCCCCACTCTGTTGAG transferase GGTATTTGAG GCTTACCAAG AGCTGCCTGC TTTCCAGGATGCTATGCCAG AAAAGCAGCC TGATGCCACT GCCTGAGGCA AGAATCTCAG GCTATCCATCTCCTTGAAAG TTCCCTTCTC AAACCGTTGA CATACCTGCT GGACTTGCAT TTCGGAGAATTGTTAGCTTT TTCTATTTCT AAAGGCATTA TGACAAGGAT GAGGATGGCG CCTGGTTTCTTCAGGCTAGA C17 GATCCCAACC AGTGCTGCTC CGCCGTGGTG putative protein SEQ IDN° 67 CTATCCATCT CCGCCCCGAT TGACGCCGTG At2g38310 [A. TGGTCCCTAGTCCGCCGTTT CGACAACCCG thaliana] CAAGCGTACA AGCATTTCCT TA C170aGATCCATGGC GGCTGTTCAC TCAGTCCTCC putative glycosyl SEQ ID N° 68GCCACGCGTC CTGTCCAGAACACGTCTTCT transferase ? TCCACTTCAT CGCCGCTGAGTTCGACGCGA CGAGCCCGCG AGTTTTGACA AAGCTGGTCC GATCCATTTT CCCTTCGCTCAACTTCAAAG TCTACATTTT CAGAGAAGAC ACAGTCCTAA ATCTCATCTC TTCATCGATCCGACAAGCTC TCGAAAACCC GTTA C170b GATCCATTTT GCCGACTTCC CTTGCCTACAprobable SEQ ID N° 69 TTGTTCCATC GACCAGAGGCTGTTCACCTT cytochrome P450GGAGACCTGA TGCGGTTATG AGTACGACCG monooxygenase GGCGTGGACG GCACTCGGTCCTCCGGATTT TCAAGGGCCG CCGGGGGCGC ACCGGACACC ACGCGACGTG CGGTGCTCTTCCAGCCGCTG GACCCTAGCC TCCGACTGAG TCGTTTCCAG GGTGGGCAGG CTGTTA C174GATCCATGAA CCCTGCAAGG GCATTTGGGC beta-tonoplast SEQ ID N° 70 CTGCTCTCGTCGGCTGGAGGTGGAGGAACC intrinsic protein ACTGGATTTA CTGGTTGGGC CCTTTTGTGGGTGCAGCCTT GGCTGGACTT ATCTACGAGT ATGGAATCAT ACAGCATGAG GCCGTTCCGCGCCCGACCAC CCATCAGCCA TTGGCACCAG AAGATTACTA AATGCACTTC GATAGCAGTCTTCCATTTGT GAATAAGAGA GGATTGTGCT TA C175ACAGCTATGACCATTAGGCCTATTTAGGTGACACT auxin-responsive- SEQ ID N° 71ATAGAACAAGTTTGTACAAAAAAGCAGGCTGGTAC like proteinCGGTCCGGAATTCCCGGGATGTACAAATTACTAAT ATAGTTTCTTCACAATTATGGAAAGAAGCGTAGCTAATGAGGCACCAAAGGCCACAATAATGGTGGAGG ACTACAAGAAGAATCTTGAGTTCATTGAAGAGGTGACTAGCAATGTTGATGAGGTCCAAATGAGAGTTCT TGCTGAAATCCTCTCCCAGAATGCACATGTTGAGTCTTGCAACGCTATAATCTCAATGGCCGCACTGATA GAGAGACATTCAAGAAAGTCGTACCTGTCATCACTTATGAAGATATTCAGCCTGATATCAAACGTATAGC CTATGGTGATAAATCTCCTATTCTCTGCTCCCAACCCATCTCTGAATTATTGTCAAGTTCTGGCACGTCTGG AGGGGAGAGCAAATTGATACCATCAACAGAGGCAGCGCTTTGGGAGGAGATTACAGCTTCTAAAACTTCT GATGTCTGTGATGAGCCAAGTGGCTCCAGATTTTGGAAAGGGTAAAGGAATGTATTTCATGTTTCATAAGT TCTGAACAGAAGACCCCAGGAGGATTACTAGCACGCTTTTTTACAACTAGTTTTTACAAGAGTCCTTATAT CAACTGCGGATACCCCTGCAGGAAATTCACTAGTCCAACGGCAACCATTCTTTGCCAAGACTCTTACCAA AGTATGTACTCGCAAATGCTCTGTGGCCTCTGCCAAAACCAAGAAGTCCTCCGTGTTGGCTCGCTTTTTGCAACCGGCTTCATTCGTGGCATCCGTTTCTTGGAGAAGCATTGGTCTCTACTTTGTAACGATATGCGAAACGGA ACCATTAACACCCAAATTACAGATCCTTCAGTGAGAGAAGCAGTGATGGAAATCCTCAAACCTGACCCAA ATTAGCTGATTTTCATTGAGGCTGAATGCAGCAAAGACTCATGGCAAGGAATCATCACTAGGTTGTGGCC TAATACCAAGTATGTGGATGCTATTTTGACTGGATCCATGTCACAATATATACCGATACTTGATTATTACAGCAATAGCCTCCCTCTTATCAGTACTTTGTATGGTTCCTCAGAATGCCACTTTGGAATCAACTTGAACCCTTTTTGTAAGCCCAGTGAAGTCTCTTACACCCTTATTCCCACCATGTGCTATTTTGAGTTCTTACCATATCACGGAAATAGTGGAGTCATTGATTCTATCTCCATGCCTAA GTCGCTTAATGAGAAAGAACAACAACAATTGGTTGATTTGGCTGATGTCGAGATTGGCCAGGAGTACGAG CTTGTTGTTACCACATATTCTGGACTCTACAGATATAGAGTCGGTGATGTGCTTCGGGTTGCTGGATACAA GAACAACGCGCCTCGATTCAACTTCCTATGCCGGGAAAATGTAATCTTGAGCATTGGTGCTGACTTCACTA ATGAAGTTGAGCTACAAAACGCAGTGAAAAATGCAGTGGGCAATCTGATGCCATTTGATTCTCAGGTAACCGAGTACACCGGCTATGTCGATATTACCACCATTTCC AGCCACTATGTCATATTCTGGGAGCTGAATGCGAATGACTCTACCCCAGTTCCTCCTTCAGTCTTTGAAGATTGCTGCCTCACAATTGAAGAATCTCTTAACTACTT CTACCGCGAGGGCCGTGCGTCTAATGCATCCATCGGGCCTCTAGAAATTAGGGTGGTGGAAATTGGAACT TTTGACAAGCTCATGGACTACTGCAGTAGCTTAGGTGCTTCCATGAACCAATACAAGACACCCCGTTGTGT CAAATATGCACCCCTTATTGAGCTATTGAACTCTAGGGTCGTCTCCAGATACTTCAGTCCCATGTGTCCAAA ATGGGTTCCTGGCTACAAGAAATGGAACAACACCAGTTAAATGTCAAGCTTCCAATTTCTCTACTTGAAGCTTCATTCTCTATCCCGAAAAAAGACAACCATTTGTGGATTATTTAGTCAATCGTCATCCTAGCTAAGTTGGTCTTTCGTGAACATGGTATGGATTTGTATTTGTCACAAATAAAATGTGGCACTTTTTATTTCTGTAATGGTTT TATTGTGTCAAGTAGTTTAGTGCAAAGACGAGGAGAAGAAGTCAAAAGAGAGGTTTGGTAGACACTTTTAGTGCCCATATTATGTTGGTGGTTTCACTTGTCTTTTCTATTGCATTTGTGAAGTCTGCTATATAATAAACATC CCGGCATCT C177 GATCCATGGCTCGGTTTTGG GCTAAATATG glutathione S- SEQ ID N° 72 TTGACGATAAGTCATATAATACCTGGAATG transferase TGTTTATGCA ACACTGGAGT C C178TGGAACGGCGCTCCTTATTTGAGGAAAGTGGACCT auxin-induced SEQ ID N° 73CAGAAACTATTCTGCATACCAGGAGCTCTCTTCTGC protein IAA4TCTACGAAGAAAGATGTTTACCTGTTTTACTATTGG TCAATATGGATC C18 GATCCCAACGCATCAGGGTG AGTCCTTCAA RNA-binding-like SEQ ID N° 74 AAACACCAGTGAGGCCACGA CTTCCCCGTG protein CCATGATGCA GTAACCGATG CTTGTTCTCATGACATGGAA AGAGTTCAGG AAAGCCTTCT TGGAAGACTT GAGGTCACCA TGGGAAGGCGAAACGAAATT CTGTTTCAGT AATTTCCACC TTTCTTTTCT TTTTTCTTTC TGTATTGCCAACACAGTAAC TTTATTGGTA CTGAACATGG CATTA C180bTAAGGCTACAAGCGTAACTTTTAGTGATAGATCAT ferredoxin-NADP SEQ ID N° 75CATGGATC oxidoreductase C181a TAAGGCTACAAGCGTAACTTTTAGTGATAGATCATferredoxin-NADP SEQ ID N° 76 CATGGATC oxidoreductase C182 GATCCATCAGTTGCTTCTAT AAAGCCATTG patatin SEQ ID N° 77 GACGTCAAACAAGTTTTGCTGCTCTCATTA GGGACTGGCA CTACTGCAGA TTTTGCTGGG ACATACACAGCAAAGGAGGC AGATAATTGG GGTCTTGTTT CCTGGCTATT TCATAATAAT TCGAACCCTCTTATTGAAAT GTCATCTGAA GCAAGTGTTA TTATGAATGAT TATTACATC GCCACCATCTATCGCGCTCT TGGTGCTGAA ACGAATTA C183a GATCCATCAA ACAAATCTGT GTCTGCAGGCauxin induced like- SEQ ID N° 78 AGCTCTTCTA ATAAGATCAGACAAATAGTT proteinAGGCTTCAAC AGCTCCTCAA GAAATGGAAG AAGATAGCAGCTGCCTCCCC CTCCTCCACCCACCTCCATA ACAACCTCCT CAGTATAAACAACAGCACAA GCAGCAGCAC CAAAAGCATCAATAAGTTCC TCAAGAAAACCCTTTCATTC TCGGAAAAGG ACAGATCATC ACCTGCAGAGGTATGCAGCATTA C185c GATCCACCAA AACCCTCGGC AACTTCGTTA rRNA intron- SEQ IDN° 79 CTCAGGACTC ATCAGACTGA GAGCTCTTTC encoded homing TTGATTCTATGGGTGGTGGT GCATGGCCGT endonuclease TCTTAGTTGG TGGAGCGATT TGTCTGGTTA C2GATCCCAGAAGTTAGGACATACGTCCCTAACGTTG lipase-like protein SEQ ID N° 80TCGCGGGGATTATGAGAGGCATCAAAGATGTGATTTCAGCTCGGAGCCACGCGCTTTTTGGTTCCAGGAATT ACCCACTCGGGTGCTTGCCGCTGTATCTCACATCATTTCCTGATAATAATACAGGCGCGTACGACCAAAT GGGTTGCTTGAGGAACTACAATGACTTCGCTTCGTTCATAATAGATACGTGAGCAGGGCTATCGCGAATC TGCAGCGCGAATTCCCGAATGTTAGCATTGTGTACGGGGATTTCTATGGTTCCCTTTTGACAGTTATGCGCAGTGCTTCTTCCTTTGGATTTGACCAGAACACGTTG CTTAGTGCATGTTGTGGAACTGGAGGGAGGTATAACTTTA C201a GATCCCGAAT GACGACAAGC TTCAATCCAT putative protein SEQ ID N°81 TACTGTAAAT GGTAGCAAAA TCCTACCCGA At5g44670 [A. TTGGGGATAC GGTAGAGTTTATACTGTTTT thaliana] AGTTATCAAT TGCACTTTCC CTATTCCAGT TGGTACTGAAAATGGAGGAA AACTCGTAAT TCATGCCGCT ACTAACGGCG GCGGGGACAC TAAATTCAACACCGCCGACA CTTTCGTAGG GTTA C201b GATCCACCTG CCCTTTCAGA TGAGTCAATCN-carbamyl-L- SEQ ID N° 82 ACTAAGGCGA CAGAATTAGC ATGTCAACAG amino acidCTGAATTTGA CTCGCAAGAG AATGATTAGT amidohydrolase CGAGCCTATC ATGACTCCCTGTTTATGGCA AGAATATCCC CGATGGGCAT GATATTCATT CCTTGTTACA AGGGATATAGCCATAAGCGT GAAGAGTTTT CATCTGTTGA CGATATCGCG AACGGGGTAA AAGTTCTAGCGTTGACTCTT GCCAAGTTAT CTCTCTCATA ATCCCTTA C202TTATAGATCAGAAATTTGAAGCCGGAGAAAATGGC dihydrolipoamide SEQ ID N° 83GATAGGGAGCTTAGCAAGAAGAAAGACCACAACA dehydrogenaseATTTTATCTTCCAGATATCTCTATAGCACATCCAAATATTCATTTTCTCTCAGCAGAAATTACTCTTCGGGA TC C203 GATCCCGAGT TGTACGCATGAGCTCGCAAA carbonic anhydrase SEQ ID N° 84 AGATCAAAGC CCAAAGTTTCTCGTATTCGC CTGCTCCGGC TCCACCAGCT GTGCCC C207b GATCCCTATC CAATAGATATGGAATTTCGA chlorophyll a SEQ ID N° 85 CCACCTTGTA TAGTTCTATC AACCATTGGAoxygenase ATCTCAAAGC CAGGCAAGTT GGAAGGGCAG AGTACCAAAG AGTGCTCTACACACCTACAC CAACTTCATG TATGTTTACC TGCATCTAAA CAGAAGACAA GGTTGTTATATAGGATGTCA CTGGATTTTG CTCCCGTGCT AAAACACATC CCTTTCATGC AATACGTGTGGAGGCATTTT GCTGAACAGG TTA C207c GATCCCTGAT GCATATGAGC GGCTGCTTCTlysyl-tRNA SEQ ID N° 86 TGATGCTATA GAAGGTGAAA GGCGGCTTTT synthetaseCATCCGCAGT GATGAGCTGA ATGCTGCTTG GTCTCTTTTC ACACCAGTGT TACTCAGGACTCATCAACAA GCATGAACTT TGCAATGCAT ACACTGAATT GAATGACCCT GTTGTCCAACGCCAGCGTTT TGCTGATCAA CTCAAGGATC GACAATCAGG TGACGATGAA GCTATGGCACTGGATGAGAA CTTTTGTACA GCTCTTGAAT ATGGATTACC TCCTACTGGT GGTTGGGGATTGGGTATTGA CCGACTTGCG ATGTTTGTTA C208 GATCCCCACC ATCAGGTATT CCGAGCCGCAtranslation SEQ ID N° 87 ATAGGTGAAC CGGACCCTCT TGAAGATCAT elongationfactor CGTATTCGAG ACCACCCCAA ACGACCCCTC like protein GTTCGATGTTGTTCTTCATT CCAACAACTG CTTCCAGCAG CCTAACGCCG GACACACGCA CACACGCACCAACAGGTCGT CGTTCTTCCA CACAAACCCG CCGGAACGGA CTCCTTCTCC ACCCAGAACAGACCCAGACC G C212 GATCCCTATG AACGAGCTTT AGCTCGTTCC auxin-induced SEQ IDN° 88 TGGGCTAATT T glutathione S- transferase C213bTAACAACGCAACCACACAGAATCGATCGTTACATA A3 [Nicotiana SEQ ID N° 89 AAGGGATCtabacum] C214a GATCCCTTGG ATGGTACTTG TTGGTGAACG histidyl-tRNA SEQ ID N°90 CGAACTTAGC GAAGGAGTTG TAAAATTGAA synthetase GGATGTGTTT GCAGCTATTGATTATGAAGT CCCCAGAGGT AACCTTGTGA ACGATTTATG CAGAGGATTA GGCATGTAATATCTCAAGTT ATTAGTATTG TTAGATTGAT ACAAGAATGC TTTTTTGGGG GGTGGGGGTT AC214b TAAGCGCAGA TGATAATGGT GAAGGGGGTA potassium SEQ ID N° 91 CATTCGCTCTTTACTCTTTG CTGTGTAGAC transporter ATGCAAAGTT TAGTCTACTT CCCAACCAACAGGCAGCAGA TGAGGAGCTA TCTGCTTACA AATATGGATT CTCCGGGCAG TCGGCATCTTGTTTACCATT GAAGAGATTT CTTGAGAAGC ATAAGAAGTC ACGCACAATA CTGCTTATTGTTGTATTGTT AGGTGCTTGT ATGGTCATAG GAGATGGTGT TCTGACTCCT GCAATGTCAGTTATATCATC AATATCAGGG ATC C215 GATCCCTCTC TATTTGCATA AATGTTGATG putativeprotein SEQ ID N° 92 GATTTGAAGC AATGTTTTTC ATAGGAGTAA At4g25640 [A.ATGCTGCTAT AAGTGTTCGT GTCTCAAATG thaliana] AGCTTGGGCT AGGACGTGCCAGGGCAACCA AGTATAGCGT CTGTGTCACA GTGTTTCAGT CGCTTCTCAT TGGGATAGTATGCATGATTG TAGTATTGGC AGTAAGAAAT CATCTGGCCA TTCTTTTCAC AAACAGCAAGGTTCTGCAAC GTCCCGTACC TGACCTGGCT TGGCTTGTAG GAATAA C216b GATCCCTAGGCATAAAACAA TGAGCAACGC putative protein SEQ ID N° 93 CGCAAGAGATATACGGAATC GCTGACCCCC At2g20240 [A. GAAAATTTTG ATCATTTTCA TTCTCTGATTthaliana] TTGAAAGAAT AGCAGCGCCG TTTTTGGAGC TTGGCAAACC GGACCCCATCCCCCTTTTTG TCGTCGTCTT TCTCAAACCA GACTCCCCTC CCTGATCATT TTTTCTTCTGGGAAAACAAA GCAGCATTTC CATGGTTTTG GCTTTA C217 GATCCCTCAA GTTGCACTTTGAATATGCTT 60S ribosomal SEQ ID N° 94 GTAATAAATA GAAGTAATAT AACAGTGCTTprotein L13a TGTTCTCCAA GGCTTCAAGG TGTGACCATG TTGGATACAA TCTGAAAGTTGTGTTCCAAT CCACGTGATC TTTCTGGCTG TACACGCTAA TCCACCAGAC AACTTGCTTACTCAGGACTC ATCAACTCGC CATTATTGCT CCAATCAAGT ACTGAAGTCT AAATATAGTTGTTTGAAGTA CAATTTTGCT GGAGATTGAT GTTTTGGCTT A C22GATCCAACTTACGACATAGGCCTATTGGAATTGGA ribonucleotide SEQ ID N° 95GTTCAGGGTCTTGCAGACACATTCATGTTGCTTGGC reductaseATGGCATTTGATTCTCGGGAGGCTCAGCAGCTAAA CAAGGACATATTTGAGACAATATACTACCATGCATTA C220 TAGTGCTATGGCTGTGGACTCAGGTGCATTTGTACA putative F1-ATP SEQ ID N°96 CAAAGAGGTATGAATGTACTAAAAATGTCACAGTC synthase subunitTCCCGCACTTCATATTCATCATTTTTGAAAGCGAGG alpha GGAAGGGATC C224a GATCCCTTTACATCATCCAC ATATAATTCA seven in absentia- SEQ ID N° 97 TTCTCAATTCCCATCTTCAA AATCACCCCT TA like protein C224b GATCCCTGGC GACAAGCAATGGAACAACAT auxin-responsive SEQ ID N° 98 GAATTGAATA GCCAATTTCT GTTAGTACCGH3-like protein C227a GATCCCTTCT TTCATATCTG AGATTCAAGC lipase SEQ ID N°99 TGCAATCTGG GGCATATACA ATAACGGTGG GAAGAATTTC TGGGTTCATA ACACAGGACGCTTGGGTTGT TTGCCACAGA GGCTTGCCAC AAGAAATGGG AGCAATTTGA ACGATTATGGATGCATTA C227c GATCCCTGTGGCTAGACTAACTGGCCGAGAGGGTT mitochondrial SEQ IDN° 100 AGCGAGGTTCCTGCTATGGTGAAGTGAAAGATCTT ATPase subunit 9TCACTATAGTGGGAAGAAGACAGGTGGGAGCGAG CGGAGCGAGAGCAAAGCAAGCTCTAGTGGTGGGTTGTCTTCGCGGTCCCATTA C228a GATCCCTTCA ACGGCGTTGC TTGCTGATGG arginine SEQID N° 101 TGTCCGTGAG GCTGCTCAGA TTTATTGTGA ATTTA decarboxylase C228bGATCCCTACG AACTCGGGAA ATGGGCCAGT putative protein SEQ ID N° 102CTTTCAGCTA TTTGATTAGA ATAATCACAC At3g59770 [A. CGATTA thaliana] C230TAATCCATGT CAAACTCGAC TTTTTGCAGC B12D protein SEQ ID N° 103 CGTAGGCGTTGCTGTAGGGA TC C232 TAAACGTGAA TATCGGATTA CACCTCCGCC proline-rich cellSEQ ID N° 104 TCCGCTGTCA ACACAAGTGG GAGACATTCC wall like-proteinTCGAAGCACA TTCAACTTTG ATTTTGACTT TGAGGGAAAG ATTCTGGCCG AAGCAGAAAAGGAAAGCCAG AATTGGAGCA GGCTAGGGCT GGAA C237b GATCCCGTCT ACCTTATTCTTTTCAGCAGC putative protein SEQ ID N° 105 CGCAACAGGC AAGTTTTTGCACCATCTGTT TA At1g22750 [A. thaliana] C238a GATCCGTCAA GTTTGCATGGTGGTTGCCCT prolyl 4- SEQ ID N° 106 GTGATTA hydroxylase C238b GATCCCGTAGAAAATGCTTC TTTTATGCCT cytochrome c-type SEQ ID N° 107 TGGGTATTTATATTATAATT TTCATTTTTT biogenesis protein GGTGTTTAGG ATTA C238cGATCCCGATGTGATTCATAACTTTCATCACACCCCT vestigial protein ? SEQ ID N° 108CTCAATATCTTCAGCTGAAATTTGTTACTCCATTTA C23bGATCCCACCTCAGGAAAAAAAATCTGCTACGTGCA cellulose synthase SEQ ID N° 109GTTTTCCACAAAGGTTTGATGGGATTGATCGTCACG ACAGATACTCAAACAGAAATGTCGTATTCTTTGATATTA C24 TAAAGCAACA AAATCAATTC ACAGCACCTC amino acid transport SEQ ID N°110 ACTTTAGTGT AAGCAAGAAT CAAAAAGCAA related protein GTTGCAGGTACAAATTCCAT AGTGCCAGCT GACCTACCAA AGTTGGGCAT AGCCCATAAC AATGTCAACATTCTCAAAAG AAGATAAAAT CACATCTGTG TTCAACCACA TCATTGAATA TCAAAAGATATAAGAACCTA TAAGCTGGGC GTTCTTGTTC CTTTTTTCCC TTTTGATGAA GGTATCTCTCCTATAAGGGT GGGGGGATC C25 TTCAACAGAA GAACTCCATC ATCAGCCACT proline richprotein SEQ ID N° 111 GAGGAGAGAA CGCCCAACCC CTGGACAAAA TAGAAAACACACAATATTGG CCGCGGACCC CAACTTCAAA AACAGAAATC GACCTTACCC AATTCCCAATTTCCAAGAGC CTCTCACGCA CACACACCCC TGAAACCTAG TAAAAATAGA AGGTCTTTGCACAAAACAAC ATCTCCAAAT GGCTCA C28a GATCCCCTGA ATATTGGGTA GCTGTTGTTA T48protein [Tupaia SEQ ID N° 112 CTCAGGACTC ATCACATGCA GAGGTATCGCherpesvirus] GTGTTTGGAT TGTGTTA C28b GATCCCCTGA ATATTGGGTA GCTGTTGTTA50S ribosomal SEQ ID N° 113 CTCAGGGCTC ATCGAAAGAA CCCCTCATCG proteinGTTGTTTATC TGGTTTA C29a GATCCCCCTGAGTTCGCCAAGGACTTACTGCCCAA heat shockSEQ ID N° 114 GTATTTCAAGCACAATAACTTCTCCAGCTTCGTTCG transcription factorTCAGTTA C3 CATAAGGAGC AGCTGATCGG AGTCCAAAGA NADPH SEQ ID N° 115GAATTCGAGA TGCTATAGCA CATATGAAAT oxidoreductase TCTGGGTAGC TCTGTTGTGTAAGGTGTTCT homolog GTACAATGAC AAACAGGATT TGTGATATTC GTTGTGTAAA AGGCAGCAC30 AGGTATTACA AAACGCATGG GGAGTAGTAG putative protein SEQ ID N° 116TACAAGGGAA AGGGGTAGAA TGTTCACCAG AT5g05250 [A. CTTGTTATTT GTTGAAGACGAGTAGAGTTG thaliana] GTGCTGGTTT AGGAATGGGA TTGTTTTGCA CTCATTTCTGTTTAGCAAGA GTACAGAATT TTAGGG C301 TACCCGAAATCCGAACTCTTGCTCCGAATCAAGCCornithine SEQ ID N° 117 AATGTTCGACGGCAACGCGAGGTGCCCAATGGGTCdecarboxylase CAAAATACGGCGCGCTTCCAGAAGAAGTCGAGCCGCTGCTCCGGGCAGCTCAGGCCGCCCGGCTCACCGT CTCCGGTGTCTCCTTCCACATCGGCAGCGGAGATGCCGATTCAAACGCTTATCTCGGCGCCATAGCCGCG GCTAAGGAAGTGTTTGAAACAGCTGCTAAACTCGGTATGTCGAAAATGACTGTTCTAGACGTCGGCGGCG GGTTTACATCCGGCCACCAGTTCACAACCGCCGCCGTCGCTGTTAGATCAGCTTTA C303 GTGGATGAAATAATGGTCATGAGTTTTTCAAATCTG putativeprotein SEQ ID N° 118 TAGACTGGGATCTGATTATGCAACTTCCCAGGCCA kinaseCCGCTTATACCTGTGCCGCACTGACGAGAATGTGA ATATTATGGAGGGAAATGAAGAAATTGCTGTGGAATTATTTCGAACAGGGAGTGTTTA C304 TAAACCAAAA GCAACTGAAC TCAAGGGCCA F1-ATPasealpha SEQ ID N° 119 CCTCTGAGAG TGAGACATTG TATTGTGTCT subunit ATGTAGCGATTGGACAGAAA CGCTCAACTG TGGCACAATT AGTTCAAATT CTTTCAGAAG CGAATGCTTTGGAATATTCT ATTCTTGTAG CAGCC C305a GATCCGAGGAAGACGAGACAGAAACACCAGCGGAheat shock protein SEQ ID N° 120 TACTTCAACAGAATCAGATGCAGGCTCTGCTGAAGTCTCAGAGGCACAAGTCGTCGAGCCATCAGAAGTA AGGACCGAGAGCAACGATTATTGGGAGTGATTTAC305b TATACAGGAC AACGACGACG ATGAGTCCTG latex-abundant SEQ ID N° 121AGTAATCAAC CGTTTCGGAT TTTCTGAGGA protein AGATATTACT GTACTAATTGATACTGATGA TTCTTACACA CAACCAACTG GTCGGAATAT ACGTAAAGCT TCGTCGGATC C306GTACTCGCGGAGAGGACTATGAATCTGACGATGGG putative protein SEQ ID N° 122GTGGAATCATGGGCCAAATAGTTCGACATCCGAAT At1g26460 [A.GGGCACAGAGTAACCGTGTGGAACATGCTGTTTA thaliana] C308GATCCGAAAGCATCACCCGAAATCCGAACTGTTGC ornithine SEQ ID N° 123TCCGCATCAAGCCCATGCTCGACGGCAACGCGAGA decarboxylaseTGCCCAATGGGCCCGAAATACGGCGCGCTTCCAGA AGAAGTCGACCCGCTGCTCCGGGCAGCTCAAGCCGCCCGTCTCACCGTATCCGGCGTCTCATTCCACATCG GTAGCGGAGATGCCGATTCAAACGCTTATCTCGGCGCCATAGCCGCGGCTAAGGAAGTGTTTTGAAACAGC TGCTAAACTCGGGATGTCGAAAATGACTGTTCTAGACGTCGGTGGCGGGTTTACATCCGGCCACCAGTTC ACAACCGCCGCCGTCGCCGTTA C309ACATGGAGGTGCTTATATTGTGAGACACGCCGCGA S-adenosyl-L- SEQ ID N° 124ATAGCGTGGTCGCAGCAGGACTTGCTCGCCGCTGC methionineATTGTGCAGGTTTCTTATGCTATCGGTGCGGCTGTA synthetaseCCACTGTGCGTGTTTGTTGACACTTACAAAACTGGA ACAATTCCAGACAAGGATATTTTGGCTCTGATCAAGGAGAACTTTGACTTCAGGCCTGGAATGATGTCAA TCAATCTTGACTTGTTA C31 GATCCCCTATTGACTGCCTC TTGCTCTTGC putative protein SEQ ID N° 125 ACTTGCATATACGCTTATAT TCAGGAATAT At1g71240 [A. GCTGTCTTAT GTTTTCCCAG CAATCTTGATthaliana] TGTCTTGGCT GCTGGCATGT TATTACTTTA C310 GATCCGACTT GCTTTGTCTCTTCGGACGAG 40S ribosomal SEQ ID N° 126 TTACTCAGGA GCATATGAAA AGGAATGTTGprotein S5 CCATACTTTT GAGTAGCAGG AAATTTAGGA TCAGTAAAAG AGGCTTACTCAGGACTCATC GTCAGGCTGT TGATATTTCT CCACTTCGCC GTGTTA C311AAACATGAGGACAAACTTAACATGAGGGGGATGC putative heat shock SEQ ID N° 127AGGTTCGGACGAAGTCTAATGAGGTACAAGAAGTC proteinGAGGCATCAGAAGTAAGGACCGAGAGCAACGATT ATTGGGAGTGATGGTTA C312 TAAGCCCCCAAACTAGAGTC TCCTCAGCTC receptor-like protein SEQ ID N° 128 CTAATCTTTGGCCTAAGAGT ATTTTGGTTG kinase TCAGAAATAC TTCAGCGCTG CTTTTTTTAC AAGAAAGTGGAAATTTGGTT TATGGTAACT GGGGTAGTTT CTTGAATCCA ACTGACACAT ATCTGCCAAACCAGAACATC AATGGCTCAA ATGCAACTTC CAGTAATGGA AAATCCAG C313a GATCCGAGACATCCAGCCGA GTCCACAAAT putative pyruvate SEQ ID N° 129 GCAACCGATGAGTCAGTATT GAAGGTTGCA kinase CTGGACCATG GGAAAACAGC AGGTGTTATA AAGCCACATGACCGAGTTGT TGTTTTCCAG AAAGTTGGTG ACTCATCTGT GGTGAAGATT ATTGAGCTTGAGAATTAGGT TTGTACATCT TTGTATGTTT CAATTGGCTG ACATTCTTAG CTTA C314bGATCCGAAAA AGAACAAGAC CAAAAGGTCT putative protein SEQ ID N° 130TGAAAAAGAG AGTGACGAGC AGAAGAGAGG KIAA0565 [Homo AAACAGAAAA TACACAAAAATTGGGAAGGC sapiens] AAAATAGTGA AATCTCCCAC AAATTTCAGC CTAAAACTAG CTTAC314c GATCCGATGG GAAGACCCGG TATGAGGATT calmodulin SEQ ID N° 131TCATTGCCGG GATGGTTGCC AAGTGATTTT TGCATGTGAT TTGCATCTCA GGCTATATTATTCATAGCAG TGAAAGAAGA GCTGACTTTT TCCCTTTGTA GCTTTA C316AGGTCTATTTTTTCACCAAACAACATTCAGTATTGG putative oxidase SEQ ID N° 132CTTTGTCCAAAGTAAAAAACTTTATACAAGATGTGC AAAAACTTGTGGTTTTACAGCCCAAGGCATTATGTGGTTTAGACCTATATAGTGGAATCCTAATGAGGTA TGTCACGGTTTCAAATGCTTACTTGGGACATCAAGAAGATGCAGTGGATTTTTGATATTACATATTATAGAA GCAAAAATCCATTGACTCCTAGGTTATATGAAGATATTCTTTGAAGAAATAGAGCAAATGGCAATGTTCAA ATATGGAGGAGAGCCTCACTGGGGGAAGAATCGTAATGTGGCTTTCATTGATGTGATTA C320 TAATGGGGGAGGCTATAGCTACAATGAATCAAATGubiquitin-specific SEQ ID N° 133 GAGGAAAATTTGGGTCCACAGTTATCTGGTCTTGTCprotease-like protein GGGTCGGATC C322TGCCCTGTTTATCGCTGCACTTTTCCCGAGATACAT RING-H2 zinc SEQ ID N° 134CCGCTACCGCATCTTCACTAACGGTAACAGCATCCT finger protein-likeCCAAACACTTTCCACGCGCCGCCGCCCTTCTGCTGC AACACGTGGACTCGACAATTCGGTCATCGACACTTTCCCCACCTTTTGCATACGCCGAAGTGAAGGATCAT CATATTGGCAAGGGTGGTTTGGAGTGCGCAGTATGCTTGAACGAGTTTGAAGACGACGAAAAGCTGCGGT TGATCCCAAAGTGTGATCACGTGTTCCACCCTGAATGCATCGGTGCTTGGCTCAAGTCTCACGTCACTTGCCCCGTTTGTCGAGCTGACCTTACTACTCCTCAACCTG ATGTTA C323ATCCCCATTGGCCTAGTTGGTTCTATGGTGATTACT amino acid transport SEQ ID N° 135ACCACTATATACTGTATATTTGGCTATAACGCTCTGT protein AAT1CTTTATGCAGCCTTATCAGAACATTGATCCTAATGCTCCGTTTTCTGTGGCGTTCAAAGCTGTTGGATGGAGT TGGGCGCAATACATTGTGGCTGCAGGTGCATTGAAAGGAATGACATCTGTATTGCTTTGTAGGCGCGGTTGGTCAGGCGCGTTATCTCACTCACATTGCACGGACTCACATGATGCCTCCTTGGTTTTCCTATGTTGATGCAA AAACAGGAACGCCCGTTA C324a GATCCGGAGAGCCAAACATT TACGTAGTTT 1- SEQ ID N° 136 TCATCATCAT GAAATGGTTA CCTGAAACGAaminocyclopropane- TTGTCAGATT CTGTAATTTT GCTGAGTATA 1-carboxylateCAGAAGACAA TTTTGCATAT AGTGCTTCAT oxidase GCTCTTACAG TTTGTATGGATCATTGTTCC TTATCGTTTT ATAATGTATT GTATCATTTT ATGAATTCAA TGTTTGGATAGATTTGTATT GTTTGTTATT GTTA C324b GATCCGGGGGTGTAGTTTGGATTGAATTGAACGGGGputative protein SEQ ID N° 137 AAGTGCATGAGTTTATTGCGTTTGATGGTTCACATGAt2g29760 [A. CTAAGTCTGAATACATTTACACCGTTTTAGATAACC thaliana]TAGTCGGTCAAATACAACACATTTACTATTTTTCCAGATGCTGATTCTTTAGTTCTTGAGAATAGCTGAAAGTAATCAGAGTTTTAGATATGCTGAACTTCCAATACAG CCTTAGTTA C325TTCACGATATCGAAACTAGCGATTACGTGAATTTCC putative subtilisin- SEQ ID N° 138TATGCTCCATTGGCTATGACGGCGACGATGTCGCC like serineGTGTTTCGTGAGAGATTCTTCTCGAGTGAATTGCAGT proteinaseGAACAGAATTTGGCTACTCCAGGAGACCTGAATTA CCCGTCGTTCTCTGTTGTTTTTACCGGTGATAGTAACGGTGTGGTTA C326a GATCCGGGAA TATCGTCTAG AAGAACTCCT anionic peroxidaseSEQ ID N° 139 CCATCGCAAC CATCAACTCA GCCAGAGGTT TTGAAGTCAT AGAACAAGCTAAACAAAGAG TAAAAGATAC TTGTCCCAAC ACGCCTGTAT CTTGCGCAGA CATCTTAGCTATTGCTGCTC GTGATTCTGT TGTTTA C326b TAACAGAAGAAGAAGAGATGCCGGCCCTAGGTTGTarginine SEQ ID N° 140 TGCGTAGACGCTACTGTTTCCCCTCCTCTCGGCTATdecarboxylase GCCTTCTCTCGGGATAGCTCTCTTCCCGCGCCGGAGTTCTTTTACCTCCGGCGTACCTCCTACAAACTCCGCC GCCGGTTCCCATTGGTCTCCGGATC C326cGATCCGGGCCGGTTCGGGTTTCGTCAACTTTACTTGA putative protein SEQ ID N° 141ATCCGGAAATGTGCTTCCCATTACTCAGGACTCATC At5g66860 [A.GTTAAACTAAGAAGTAAGATGACTGTACTAGCACT thaliana]CCTATAACTAAAAAGTAACTAGACTCATTCATCAA TATCACTCGCTCTCTCTCTGGTTA C327aGATCCGGGTTGTATTAGATATGGTTTATTACGTTA cytochrome b SEQ ID N° 142TTTTGTACTTTATTTTGAACTTCATTTCTGTTTGATTGGTTCTACTAATTTGAATTGGTTACTCAGGACTCATCAGTCCAGTGGTTCAGTGCCTAGTTTTCAAATTGAA GGTCGGGTGTTA C327b GATCCGGCATGTCTGCTCGA CAAATGGGGA 60S ribosomal SEQ ID N° 143 GGGAGCTGCT ATTAGTATACTCAGGACTCA protein L21 TCACGAAAAG GCAACCCCTA GGACCCAAAC CAGGTTTCATGGTTGAAGGC GCTACATTGG AGACTGTTAC CCCCATACCA TATGATGTGG TTA C328aGATCCGTCGG TCAGAGTGGG AGGGGCCCGC putative protein SEQ ID N° 144AAGCACATGT CGAAAATCAG GATTGATGTC At4g24290 [A. AATGCTGATC AGCACCCCTTTCAGTACGAA thaliana] ACTAAATCAA CCACAGAAGC CAGCTAAGGT GGACCTGAACTCCGCAGTTT ATCCTGGCGG TCCACCTTCA CCGGCAAGGG CGCCAAAGAT GTCGCACTTTGTCGATACAA CAGAAATGGT AAGAGGACCT GAGGAGTCAC CTGGCTACTG GGTGGTAACTGGTGCAAAGC TATGTGTAGA AGATAGTAGG ATAAGAATGA AAGTGAAGTA CTCGCTCTTA C328bGATCCATGCT TGGTGGTATT GGTTCTACCA putative protein SEQ ID N° 145TAGCTCAAGG GATGGCCTTT GGTACTGGAA AC087851 [Oryza GTGCTGTGGC ACACAGGGCTGTAGATGCGG sativa] TCATGGGTCC ACGCACCATT CAACACGAAA CTGTTGCTTCCGAGGTACCT GCTGCAGCAG CAGCTCCTAC AACCATCGGT GCTGGGTCTG ATGCTTGCAGTATGCACTCT AAAGCGTTCC AAGACTGCAT CAATAGCTCT GGAAGCGACA TTGGCAAGTTTCAATTCTAC ATGGATATGT TGCCCGAGTG CAGGAGGAAC TCAATGCTGA ATGCTTA C329aGATCCGGCTA TGTTGCTGAT CAATCTGGTT putative protein SEQ ID N° 146ATGGCATGGT TGATCCTTCT CAGCATTATT At3g63460 [A. ATCCGGAGCA ACCATCCAAGCCGCAGCCAA thaliana] GCATTTCGAA CAGTCCCTAT GCCGAGAA C329bATGGTTACTGGTTTCTATAGCCAAAAGCAAAGAGG ambiguous hit SEQ ID N° 147CTTTGGTGAGAAAGATGAAGCTTTTTGGAGGGTAT TGCTGCGTTTTTTTTGTTTGGCTTCTCTCCGGATCC330 ACGGGGGGGG GGGGGGGGGG GGACTTGAAG ethylene-responsive SEQ ID N° 148ACTGGGAAGC TCCATTAACG AGCTCCGACA element binding ACTCAACAGC CTCTGATTTAAGCCGAAGCA factor ATAGCATTGA GTCCAACATG TTTCCTAATT GCTTGCCCAA TGAATATAATTATACAGCTG ATATGTTTTT TAACGATATC TTTAATGAAG GCATTGTTGG CTATGGATTTGAGCCAGCTT CTGAATTTAC ACTCCCCAGT ATCAAATTGG AGCCAGAAAT GACTGTACAATCACCTGCAA TATGGAATTT ACCGGAGTTT GTGGCGCCGC CGGAGACGGC GGCGGAGGTGAAACTGGAAC CACCGGCGCC GCAAAAGGCA AAGCATTATA GGGGAGTGAG AGTGAGGCCGTGGGGGAAGT TTGCAGCGGA AATTAGGGAT CCGGCAAAGA ATGGGGCAAG GGTGTGGCTGGGTACGTATG AGACGGCAGA GGACGCAGCG TTTGCTTATG ACAAGGCGGC GTTTCGCATGCGGGGGTCAC GTGCATTGCT TAATTTCCCG TTAAGGATTA ATTCTGGTGA GCCTGATCCCATTAGAGTTG GTTCTAAAAG GTCATCAATG TCGCCGGAGT ATTCTTCTTC TTCATCGTCGTCGGCGTCGT CGCCGAAGAG GAGGAAGAAG GTATCTCAAG GGACGGAGCT AACGGTGTTATAGGTCCCAA CTGGGTTCTG TGTAGTGATT AAGAAAAATA GAATTAGTCG AGGGAATTTGTTTTTTACTT GGCTGAAGTA ATGAATTTGT TATTTATTTA TTTTTTGACT GTGGTTGAAATTGAATCAAA AAAAAAAAAA AAAAAGTACT AGTCGACGCG TGGCCTAGTA GTAGTAGA C331GGGTGACACT ATAGAATACT CAAGCTATGC putative protein SEQ ID N° 149ATCCAACGCG TTGGGAGCTC TCCCATATGG At3g62270 [A. TCGACCTGCA GGCGGCCGCGAATTCACTAG thaliana] TGATTAGCGG ATAACAATTT CACACAGGAA ACAGCTATGACCATTAGGCC TATTTAGGTG ACACTATAGA ACAAGTTTGT ACAAAAAAGC AGGCTGGTACCGGTCCGGAA TTCCCGGGAT GTGTCCTTTT CCCAATGTTG ATCATGCTGC TTGTCCCAGTGCGCCAGTAT TTGCTTCCCA AGTTTTTCAA AGGAGGACAT TTGCAAGATT TAGACGCTGCAGAATACGAA GAAGCTCCTG CAATAGCTTA CAATATGTCC TATGGAGATC AAGATCCTCAGGCAAGACCT GCCTGCATTG ATAGTAGTGA AATTCTTGAT GAGATAATCA CAAGAAGCCGTGGGGAGATC CGGCATCCAT GCAGCCCAAG AGTGACTAGT TCCACTCCTA CCAAACTTGAGGAAATCAAG TCTATGCACA GCCCACAGTT AGCACAAAGG GCTTACAGTC CAAGAGTCAATGTACTAAGA GGAGAAAGGA GCCCCAGATT GACGGGCAAG GGACTTGGAA TAAAGCAAACTCCTAGCCCC CAGCCATCTA ATCTGGGTCA AAATGGTCGT GGTCCGTCTT CTACCTAG C332GAGATGTCGTTTCTTGGAATTCCGATGGGACGGCG putative heat shock SEQ ID N° 150TTTGTTGTGTGGCAGCCGGCGGAATTTGCTAGAGA transcription factorTTACTTCCAACTCTCTTCAAACATAGCAACTTCTCCAGCTTTGTCCGGCAGCTCAATACCTATGTATGTTATCCTTCTATTTACTGTCTAAAAAAATTTATTCTTATTC CGTGTTTGCATTA C333 GATCCGATGAAAACGATGTC GTTGTAATCG ferric SEQ ID N° 151 GCGGTGGTCC CGGCGGCTATGTGGCGGCGA leghemoglobin TCAAGGCCGC TCAGCTCGGG CTGAAAACTA reductaseCTTGTATTGA GAAACGTGGT ACCCTCGGTG GTACTTGCCT TA C334GGGGCAAGGGAGTGGCTGGGTACGTATGAGACGG ethylene responsive SEQ ID N° 152CGGAGGACGCAGCGTTGGCATACGACAAGGCGGC element bindingGTTTCGCATGCGGGGGTCACGTGCATGGATTA factor C335c GATCCGTCAA AACCCTCGGCAACTTTGTCA 40S ribosomal SEQ ID N° 153 AGGCAACCTT TGATTGTTTA protein S2C336a GATCCGTTCG TGTATCCTGT GTTTCAAGCT cytochrome P450 SEQ ID N° 154GGACCTAGGG TTTGTTTAGG GAAGGAAATG GCATTCTTGC AGATGAAGAA GGTGGTTGCCGGAGTTCTAC GGCGGTTTAG GGTGGTTCCG GTGGTGGAAA AAGGTGAAGA GGAGCCAGTGTTGATAGCTT ACCTTACTAC TAGGATGAAG GGTGGTTTCT TGGTGAGGAT TGAGCAAAGGACAAATTGAT AGGACCCACA CTCCCTTCCC TTACAATAAT AAAATCTCCG TTA C336bGATCCGTACT GTACTTTTGA GCATTCAAGC ubiquitin SEQ ID N° 155 ACTTTTGAGTGCTCCAAACC CGGATGATCC conjugating enzyme ACTCTCTGAA AACATTGCAAAGCACTGGAA GTCAAATGAG GCTGAAGCTG TTGAAACGGC CAAGGAGTGG ACACGCCTATATGCTAGTGG TGCATGAAGA CATAGCAACG AGATATTCAA AAATAACAAA AATTATGGAATGTATTCTAT TGACTTGCTT ATCAATATGA CACTTCGGAC GGCTGTTA C338GGGAGGGGCCCGCAAGCACATGTCGAAAATCAGG putative protein SEQ ID N° 156ATTGATGTCAATGCTGATCAGCACCCCTTTCAGTAC At4g24290 [A.GAAACTAAATCAACCACAGAAGCCAGCTAAGGTGG thaliana]ACCTGAACTCCGCAGTTTATCCTGGCGGTCCACCTT CACCGGCAAGGGCGCCAAAGATGTCGCACTTTGTCGATACAACAGAAATGGTAAGAGGACCTGAGGAGT CACCTGGCTACTGGGTGGTAACTGGTGCAAAGCTATGTGTAGAAGATAGTAGGATAAGAATGAAAGTGAA GTACTCGCTCTTA C339 TAAGCAGCTCAATTCCGATC TTCACTGGTC putative serine-rich SEQ ID N° 157 TGAGACGGCCCTCTGTTCAA GTACCCCTTC protein TTCTACTCGA GCCTCGGCAG AGCCTTTTTGATCTCATTCG TATTCTAAGG AATTCTAAAG GACTCTTTCA TATTGCACCG GAGCTGGAAAAGATTGGACT ATTCCCTAGC GAGACAACA C34 AACATTCGCATTAGCAACAAAACATTCCTACACATambiguous hit SEQ ID N° 158 CGTAACAGAATCAAGCATTCATAATATTGTAATAGAACCAAAACAAAATGAAAGAAGTAATTCACCACCA AAAATGGAAACCTCGAACCAGACCAGAAAACCTGCCAGAACCGCAACAAAACTCCACAACGGGCCTCAT CGGCACCTCAGATTTGCTCGATTTCTTTTGGAGATGCGACTGCGTG C341a GATCCGTGGC TCTAAGGCTC GGCTCAACTT putative ethylene SEQID N° 159 GCCTCACTTA response element binding protein C341b GATCCGTGATGGACTTCTTC AGGCTTCTGT hypersensitive- SEQ ID N° 160 TTAGCTTA inducedresponse protein C347a GATCCGCAAG GGACCTGCAC CATATAATCT porin SEQ ID N°161 GGAGGTGCCT ACTTATAGTT TCCTGGAAGA GAACAAGTTA CTTATTGGTT ACTCAGGACTCATCGTAGAC TGCGTAGTGA TCTTCTGTAC AGGGACTATG TCAGTGACCA TAAGTTCACCGTCACTACCT ATAGCTCAAC CGGAGTGGCT ATTACCTCAT CTGGTCTGAA GAAAGGTGAATTATTCTTAG CCGACGTTA C347b GATCCGCCCAGGTCAAGATGTTACTGTACGAACAGcytoplasmic SEQ ID N° 162 AAACTGGAAAATCCTTCACTTGCACAGTGCGGTTC aconitatehydratase GACACCGAGGTGGAGTTGGCTTATTTCAACCATGGAGGTATTCTGCCATATGTCATTCGTCAGTTGACTAAGCAATAAGGGACCGTTTTGATAATTTGGCCACCTTC ACGAGCTGCTGGTGCTTA C348 TAACCCCAAAAAGACGAATA TTGTGGTGTT putative ribosomal SEQ ID N° 163 CTAACAGCGGCAGATCAAAG AAGAACTTGA protein TGAGCGAAAT CCGCTGACAA AAAAAAGAGAACTTTTTGAA TTCCGATGCC TAGCGTCCCC TGATAACCTA GGATTAGTGG TGATAGGGCTGATGTGGTAT CTCGGAAACT GGGATTTGAT GGTATCTGTA GAGCGGATC C349a GATCCGCATGACCTTTGTGA GCAACACCCT arogenate SEQ ID N° 164 GATGTTATTC TCCTTTGTACTTCAATTATA dehydrogenase TCTACTGAAC CTGTCCTTAG ATCACTCCCT ATTCAAAGGCTAAAAAGAAA CACATTGTTT GTTGATGTTT TGTCTGTTA C349b TAACATTCCC AGCAATCGATCACAACTACA putative membrane SEQ ID N° 165 AGAAGAGCAA AATAACTATGAGAAGATGTT protein ATCTTCAGCA AATTCAGTCA GACCCATTCT [SaccharomycesTATTACTCCA TTATGTGCCG CTTGCGCAAG cerevisiae] CCCACAGGCA GTGGCGGATC C349cGATCCGCAAA AATCAGAACC TGGAACAATC nucleoside SEQ ID N° 166 AGAGGTGAGTTAGCTGTTGT AGTCGGAAGG diphosphate kinase AACATCATCC ATGGAAGCGATGGACCCGAG ACTGCCAAGG ATGAGATCAA ACTATGGTTC AAACCAGAAG AGTTGGTTA C350TTCTCAGCCAGCCGTGGAACTACAAAGGCCACTCC putative protein SEQ ID N° 167ATCTAAGGCAAAGTATAGACCTCTGGAGACAAGGG At3g52110 [A.GTATCCTTCAAGAACTGGAACAGAGCAGCAATGAA thaliana]GAGAAGAGAAAGGAAGATCAAGGGAAGATGATGA GTAATAATCAACAAGGACAGAGAGGTGGTGCTATTGTTGCTGAAAAAGAAGCTGCTGCTAGAGCTTTGGA TGTCTTCTGGTTCTTGAAACCTTGCACTCTTTCCAGCTGAAATGGTCAAAGCCCACTGCTGCAGAACATTT CATGAAGTGATTCTTTCATACTTA C351aTGACTGCGTAATGATCCGCTATLTTCCACACAGAGG stromalin 3 SEQ ID N° 168ACACCTATTGGACAATCTTCCACCCTTCCATTTCTG CCGACAGTGTTGAGCTCAAAGAACGGCAAAGGAAAAATGACCCCACTCAATTCCAAACTTCAGTTCGTCA CTTTTCCTCTAAGCAACCCAATTAGCTTA C351bGATCCGCCAA AAATACAATA ATTATGAAGG ambiguous hit SEQ ID N° 169 ATGCGACACGCACACCGAGA CATTTTCGGA GAGTGCGAGC AACATAGGTT GGAATATTTA CAGCCTTAGGAGGCTTCAGG AATAATGTAT AACAACGTTT TCTTTATTGC TTTATTTTCA CTTCTCTTA C352bTAAGGGTTCA ACCTTTAGTT CTTACGATTG muconate SEQ ID N° 170 CGTACCCATTGCATTGGAAT TATACGTAGG cycloisomerase TGGAAACCTT GGATTCCCAG CATAGGCGGA TCC352c TGACTGCGTA GTGATCCACC AAAACCCTTG 40S ribosomal SEQ ID N° 171GCAACTTCGT TA protein S2 C353a ATGAATCCAG AATACGACTA TCTTTTCAAGGTP-binding SEQ ID N° 172 CTTTTGCTTA TTGGAGATTC TGGTGTTGGC proteinAAATCATGTC TCCTCTTGAG ATTTGCTGAT GATTCATATC TTGAGAGTTA CATTAGTACCATTGGTGTTG ACTTTAAAAT CCGCACAGTT GAGCAGGATG GGAAAACCAT TAAACTTCAAATTTGGGATA CTGCTGGTCA AGAACGTTTT AGGACAATTA CCAGCAGCTA CTATCGCGGTGCTCACGGCA TAATTGTTGT CTATGATGTA ACCGATCAAG AGAGTTTCAA TAATGTCAAGCAATGGTTGA GTGAAATTGA TCGATATGCA AGTGATAATG TGAACAAACT TCTTGTCGGAAATAAGTGCG ATCTCACAGC GCAGAAGGTA GTTTCCACAG AGATAGCTCA GGCTTTTGCTGATGAGATCG GCATTTCCTT CATGGAAACT AGTGCGAAAA ATGCCACCAA TGTGGAACAGGCTTTCATGG CTATGGCTGC TTCAATCAAG AACAGAATGG CAAGCCAACC AGCATCAAGCAATGCACGGC CTCCAACTGT GCAGATCCGC GGACAACCTG TCAACCAGAA GAGCGGTTGCTGCTCATCTT AA C353b GATCCACCAAAACCCTTGGCAACTTTCGTTTA 40S ribosomal SEQID N° 173 protein S2 C354 AATACGATCCCACTATACATATCGATATACATAG putativeSEQ ID N° 174 AGATTCACCGACTACATTTCAGCCATCCAGCGATC oxidoreductaseCTGATCTATTTGAAAATTGTTAGAATTGATATATCCATATATCATATTTCTGCGGGCATAAGAGTTTTTTCCTTTATGTTCGGTGGAAATCACATGTTATACTATATT CCAATAAATAGATATCTGTGTTATGATACAAGTCCACGTTTTCAAAAAAAAATGGATGAGATTGGGTCCC AGCGGATC C355a GATCCGCCGC TAACACCTAAAACACCCCCC protein kinase SEQ ID N° 175 TCCCTTGAAG CTTCTTCTTC TTCGAACCCACCCACCTCGG CCGTTACCCC TCCTATTA C356a GATCCGCAAC TAATGCTCTT ATCGGTGCAGglutamate/aspartate- SEQ ID N° 176 TCAGTGCTAT AATTTTCTGT GGATACATTGbinding peptide TATATGACAC AGACAACCTG ATTA C356b GATCCGCCGC TAACACCTAAAACACCCCCC extensin SEQ ID N° 177 TCCCTTGAAG CTTCTTCTTC GAACCCACCCGCCTCGGCCG TAACCCCTCC TATTA C358 GATCCTAGTT TGGAATATGA GCTCTCTGCTputative potassium SEQ ID N° 178 CTTCGAGAAG CCACAGAATC TGGATTTACAtransporter TATTTGCTTG GACATGGGGA CGTGAGGGCG AAGAAAAACT CTTGGTTCATCAAGAAACTG TCAATAAATT ACTTCTATGC ATTCATGAGG AAGAACTGTA GAGGAGGCGCTGCAACAATG CGTGTTCCTC ACATGAATAT TATCCAGGTG GGAATGACAT ACATGGTTTGATCTTGGTAC CATTTAGCTT CTTGCTGGCC TTGTAAGTGC TGCATTA C359CTGTACAAGTGATGAAGTGCCCTTCACGGTTTCCTC AtSIK-like protein SEQ ID N° 179TGCAAGAACCAGTGGCAGTTGGTGGTAAACATATG kinaseTCAAAGTCTCCAAGTATGACTGGAATCATCACCCCTGCGCCAAGGTTGAGTTTCTCCCCTTCCTTACCTATCACCCGAGGATCGGCTTCTCCCTCAAAGTCTTCTACG CAGCCCTCGTCTCGTCCTTCATTA C360CCACGCGTCC GCCGAAATTC TGAAGCAATA putative protein SEQ ID N° 180ACAAAGAATG GGTTGCATCG AAAAGGATCC At4g14710 [A. AGGAGAGGAC GTCGTACAGGCATGGTACAT thaliana] GGATGACAGC GATGAGGACC AGAGGCTTCC CCATCACCGTGAGCCAAAGG AATTTGTGTC TCTTGACAAA CTTGCTGAGC TTGGAGTGCT CAGCTGGAGACTTGATGCTG ACAATTATGA GACAGAGGAG GAGTTGAAGA AAATTCGGGA AGCTCGTGGCTATTCTTACA TGGATTTCTG TGAGGTTTGC CCTGAGAAAC TACCGAATTA TGAGGAGAAAATCAAGAACT TTTTTGAAGA ACACCTGCAC ACCGACGAAG AGATCCGTTA CTGTGTTGCAGGAAGTGGTT ATTTTGATCT CCGGGATCGG AATGATGCTT GGATTCGTGT CTGGGTAAAGAAAGGTGGAA TGATTGTTCT GCCTGCTGGA ATTTATCACC GCTTCACACT TGATTCAGACAATTACATTA AGGCAATGCG ACTCTTTGTT GGTGACCCAA TTTGGACTCC ATACAATCGCCCACATGACC ATCTCCCTGC AAGGAAAGAA TATATTGAAT CGTTTATCCA AGCAGAAGGCGCTGGCCGTG CAGTTAATGC TGCTGCTTAA ATTTACTAGA GGCGAAGAAG TTGAAATCCTTATAGGCTGT AATAAATGTT ACCATATGAT GGTTGTGTGG TTCCTGAAGT GTGCGCCTGGCTCAGCTTGT TGAATGTTGT AATTCGAGCA CTAAATAAAT CTCCTATGGG GATATTGAACTTAATAGTTA TATACACCTG GAGTCTATGT TGTGAATTTA AACATTTGTG CATGTCGAGTGGTACAATAT TTCCTGTTTC GGGGCGTAAT TAGCTCTGCC ATTTTTGTTG TTGGATTGCAATGACCTTGA ACTTCTTGAA CTTAAAAAAA AAAAAAAAA C364a GATCCGGGTC ACTTCCCTACATTGGGTGGC probable SEQ ID N° 181 AAGTGATGCT TTATTAGTGC TTTTCTCCCAtranscription factor CGTCCAAGAG GCAAATTGAC TGAAAAATAA C364b GATCCTCAAGCATTTATTCG CCACTTTTAC heme oxygenase SEQ ID N° 182 AACACATACT TTGCGGATTCAGATGGAGGT CGCATGATAG GGAGAAAGGT GGCTGAAAAG ATACTCTGAC TGCGTAGTGATCCGGCTATG TTGCTGATCA ATCTAGTTAT GGCATGGTTG ATCCTTCTCA GCATTATTATCCGGAGCAAC CATCCAAGCC GCAGCCAAGC ATTTCGAACA GTCCTTATGC TGAGAATTATCAACAACCAT C364c GATCCTCAAG CATTAATTTG CCACTTTTAC heme oxygenase SEQ IDN° 183 AACACATACT TTGCGCATTC AGCTGGAGGT CGCATGATAG GAAGAAAGGT GGCTGAAAAAATACTCAACA AGAAAGAGCT GGAATTCTGA CTGCGTAGTG ATCTTGGAGT GAATATGGACGAGGACTACT TACTGCGAAA TGCTAGTAGT CGGTAATTCT TCTTCCTCTG TTGATGCTGTGGAGAGAGCT AGAGCGTGGG G C365 TTGACAGGATCGATCATGCCAAATTCTTCATCATCTputative protein SEQ ID N° 184 TCTTCGCTAATTCCAAACGAGTCCACGCTGATGGAAt1g26190 [A. AGAGCTATCTAATGTTGCACCTGGACAACGTCAAA thaliana]TTATACATCAGTTGGACAATCTTAGCAATCTTCTTC GCGACAGGCTAGGAGAACAATCTCGGCAATCAAGAAAAAGCAAGAGAAGAGATATTACCGATATTTGATTCGATCAGAGTGCCTCTCATTGTAACCTTAGCAGTTGG TGGATTGGGATTATTTTTGTTTA C366aGATCCGGGAA GTTTGGTCCG ATAATATTGA CCR4-associated SEQ ID N° 185TTCTGAATTT GAGCTTATAC GAACAGCTAT factor TGATCAGTAC CCTTACATCT CAATGGATACTGAATTCCCG GGCGTTATTT TCAAGCCGGA GGTTTGGTCT TTCCAGCAAA ATCGCCGGCGACATGGACAA CATTATAAGT TGTTACTCAG GACTCATCAA CTAATGAGGA AACCGCGAAATCTGTATACT TTCTAAAACC CCAAAAGGTT TGCTCTTTCA GTTTTA C366b TAAAGCTAGCGGGGTTAGTG ATATCCTTGT 6-phosphogluconate SEQ ID N° 186 TGACCAGTCCGTGGATAAGA ATCAGTTGAT dehydrogenase TGACGATGTG AGAAAGGCAC TTTATGCATCCAAAATATGT AGCTATGCTC AGGGCATGAA TTTGATAAGG GCAAAGAGCG TTGAAAAAGGATGGGATTTG AAACTAGGGG TGCTTGCTAG GATTTGGAAG GGTGGTTGTA TTATCCGTGCTATATTTTTG GATCGCATCA AGGGGGCTTA TGACAGAAAC CCGGATC C367 GATCCGGCATGTTTTTTTAC TCAGGACTCA ambiguous hit SEQ ID N° 187 TCGTTAAAGA ATCAAAGGTTCAAGTGAAAT CATGCCCCGT GCTCCTAAAG TACGCTTTCA TATTTGGGAA CACTTTGAGGTGAAAGAAGA TAACGGAGAA GTTCGCAAAG TAAAGTGCAA GCAATGTGGT CCAGTCTATAATTTCATCCA AAGAGGGATG GCACATATTG TTTA C368b GATCCCGAGC AGGAGAGCGATAACATTGTT ankyrin like protein SEQ ID N° 188 TTAGTCGTGC AAAAGAAGTTGTGGCTCACA AGTGGAAGCA TCAGAGATAC AGAATAGACA GTAGAGTTTG AACACTTCTTCCTGACTCTG CCTTTAGGGA C369 GATGAAGAAGCTGCAATTGCTTATGATAAAGCGGCethylene-responsive SEQ ID N° 189 TTATCCAATGCGCGGTCCAAAGGCTCATTTAtranscription factor C4 GTTTGACAAT GCCTACTTCA AAAATTTACA peroxidase SEQID N° 190 GCAAGGTATG GGACTATTCA CATCATGATC AAGTGCTTTA CACGGACGGGCGGTCCAAGG GAACTGTCGA CATTTGGGCT AGTAACTCAA AAGCATTCCA AAACGCATTCGTCACTGCAA TGACAAAGCT GGGCCGTGTT GGTGTGAAAA CTGGGAGGAA TGGAAATA C401GATCCTATAG CCAACCTAAC AATTTACCCC putative protein SEQ ID N° 191TCTTCGGATC GGTTCTTGTT GGAAAAGATT At2g44230 [A. CAAAAGGAGA CGCGCTAAAGATCCCAATTG thaliana] ACTATACACT TGTATGGAGT AGTGAGAACT TGAATATCAAGCAGGATAGT GTTGGCTATA TTTGGATGCC AATTCCTCTT GAAGGCTATA AAGCCGTAGGCCACGTTGTA ACAACGTCGC CTCAAAAGCC TTCTCTTGTC ATAATTCGTT GAGTTCGTTATATTTTA C402 GGTGCTTATATTGTTAGACAGGAGGCAAAGAGTGG S-adenosyl-L- SEQ ID N°192 GGGCGCCTCAGGACTTGCTCGCCGTTGTCCTGTGCA methionineGGTTCCTTATGCTATCGGTGTGGCTGAACCACTTTC synthetaseCGTGTTTGTTGACACTTACAAGACTGGAACAATTCC AGACAAGGATATTTTGGCTCTGATCAAGGAGAACTTTGACTTCAGGCCTGGAATGATGTCAATCAATCTTG ACTTGTTA C408ATGCTCTTCTCCTATTCATTTGACTCACAATGTATC beta-glucan binding SEQ ID N° 193CTCCATAATTTCTAATGGATTCTCGGGTGTAATACG proteinAATTGCTCTCTTGGCTAATTCTGATCGCCAATGTGAGAAAATTCTTGATCAGTACAGCTCGGCTTATCCCGTGTCTGGAAGTGCAACTTTGAGGCCTTTTGGTCTTAGTTACAAATGGGATGTGAACGGTAAAGGCAAGTTTGCTTATGCTTGCTCATCCTCTACATCGCCGACTTCTTTCAACAGCAGATTCTTCAGTAACTATTTTGGATGATTTCAAGTATAGGAGCATGGATGGTGAGCTTTGTTGGCG TTGTTGGAAATTCGTGGGAGCTTGAAACGGATTCAATTCCAATATCATGGCATTCGGTTA C409a GATCCTACTAAGGTGGACATGAGTGGTGCTTATATTS-adenosyl-L- SEQ ID N° 194 GTTCGACAGGCAGCAAAGAGTGTGGTCGCCTCAGGmethionine ACTTGCTCGCCGCTGTATTGTGCAGGTTTCTTATGC synthetaseTATCGGTGTGGCTGAACCACTTTCCGTGTTTGTTGA CACTTACAAGACTGGAACAATTCCAGACAAGGATATTTTGGCTCTGATCAAGGAGAACTTTGACTTCAGGC CTGGAATGATGTCAATCAATCTTGACTTGTTAC409b GATCCTCTGA GGCTATTATG CTTGCTGGAT glutamate SEQ ID N° 195AGCTTTTCAA GAGAAAATGG CAAAATAAAA decarboxylase TGAAAGCCCA AGGCAAGCCCTGTGACAAGC CCAATATTGT CACTGGTGCC AATGTCCAGG TGTGTTGGGA GAAATTTGCAAGGTATTCTG AAGTGGAGCT AAAGGAAGTA AAGTTGAGTG ATGGATACTA TGTGATGGACCCTGAGAAAG CTGTGGAAAT GGTGGATGAG AACACAATTT GTGTAGCTGC TATGTTGGGTTCCACACTCA ATGAGATAAA TTTGAAGATG TTTA C410 GATCCTCAAG GCCCCAAAATTTGATATCGG 40S ribosomal SEQ ID N° 196 CAAGCTGATG GAGGTTCATG GTGACTATTCprotein S3a AGAAGATGTT GGCGTGAAGT TGGATCGACC AGCTGATGAG ACCGTTGCTGAGGCAGAACC TGAGATTCCT GGAGCTTAGA CTTGTTTGAT TTGGATTCTG TCTGAATATGGTGCTTGTCT TCTAAATTTA TGAATTTGTT TTAGTTGAGG TGTCAAAGGC GCGGCCTAACAAAATATTGG ATATCTTTCT TTGGTTACGT TTGATGTTA C414c TAAGCATACA TAGAAGTTACACTGCTTTCA DNA polymerase ? SEQ ID N° 197 TCTCACTCGT TGTAGTGCAGATCATACACT GGCTATCTTT AGCACCTAGA GAATGAAGCA TCATCTGATG CCTTTACTGAATTTGCTTTT CAAAACTTCC TGTAATTGCT AGGATC C417aTAAGCACCGTTTAGGAGATTTATTCTACCGTTTGGT vacuolar H+- SEQ ID N° 198GTCCCAAAAGTTCGAGGATC ATPase C418 CCTTGGTGGAGCTTGCGGTTACGATAACCCTTATGexpansin SEQ ID N° 199 ACGCCGGATTTGGAGTAAACACAGCGGCATTGAGTAGCGCACTGTTCAGAAATGGAGAAGCTTGTGGAGC TTGCTACACAGTAAGATGCAACCGCAAACTCGATCGTAAGTGGTGCCTCCCACATGGGGCCGTCACTGTG ACGGCCACCAATTTTTGCCCTCCGAACAACCACGGAGGGTGGTGTGATGCACCACGACAACACTTTGACA TGTCCATGCCCGCTTTCCTTCGCATTGCTCGACAAGGCAATGAAGGCATTGTTCCTATTCTCTACAAAAGG GTGTCATGTAGGAGAAGAGGAGGAGTACGTTTCACATTA C419 GGATATGAGCTCTCTGCTCTTCGAGAAGCCACAGA putative potassium SEQ IDN° 200 ATCTGGATTTACATATTTGCTTGGACATGGGGACGT transporterGAGGGCGAAGAAAAACTCTTGGTTCATCAAGAAAC TGTCAATAAATTACTTCTATGCATTCATGAGGAAGAACTGTAGAGGAGGCGCTGCAACAATGCGTGTTCCT CACATGAATATTATCCAGGTGGGAATGACATACATGGTTTGATCTTGCTGCCATTTAGCTTCTTGCTGGCC TTGTATGTGCTGCATTA C420CAAGTGGACAGAAGTGGTGCTTATGTTTGTGAGACA S-adenosyl-L- SEQ ID N° 201GGCAGCAAAGAGTGTGGTTGCTGCAGGACTTGCTC methionineGCCGCTGTATTGTCCAGGTTTCTTATGCAATTGGTG synthetaseTGGCAGAACCACTCTCCGTGTTTGTTGACACTTACAAAACCGGAACCATTTCCAGACAAGGATATTCTGGCT CTGATCAAGGAGAACTTTGACTTCAGGCCTGGAATGATGGCAATTA C421 CCAATCCGATATAGCCGATGGCTTCCATGAATAT acyl-CoA oxidase SEQID N° 202 ATTAGGCCACTACTCAAGCAGCAACTGCATACTGCTCGACTGTGAAGGAGAGTTGCATATATTTATAGC TGTTGTATTGTGCTGTGCCAATAAACTAAAATTGAAATATCATCTTTCTTTTGGATGATGGCCTCCTTTAT GACTTACATAGCGGTGATTA C422GACAAAACACTTGGATCCTGACAATTATCTGCTGA putative annexin SEQ ID N° 203TACCCAGCACTAGGAATGTTCATCAGCTTAGAGCA ACTTTTGAGTGCTATAAGCAAAATTACGGATTCTCCATCGACCAGGACATTA C423a ACTAGTGATTGACTGCGTAGTGATCCTGCTGGTCCG spermidinesynthase SEQ ID N° 204 GCTCAAGAGCTTGTGGAAAAACCATTCTTTGCAACGATAGCAAGGGCATTA C423b ACTAGTGATTGACTGCGTAGTGATCCTAAGAAAAT putativeprotein SEQ ID N° 205 TGCCCGTGTGATGGACCGACGACTTGAAGGTGAAT kinaseACCCGATTA C425 GGTGCTATTACAATTTTGGACACATCAAGTGATCCA vacuolar H(+)- SEQID N° 206 AGGACACTTGCTGTTGCTTGCTATGATCTATCACAG ATPase subunit-likeTTCATTCAGTGCCATTCTGCTGGGCGAATCATAGTG proteinAATGACCTCAAAGCTAAGGAGCGCGTAATGAAACT GTTGAACCACGAGAATGCAGAGGTCACAAAAAATGCCTTACTCTGTATCCAAAGGCTTTTCCTAGGTGCCA AGTATGCTAGCTTTTTGCAGGTTTA C426aGATCCTCAAG GCCCCTAAGT TTGATATTGG 40S ribosomal SEQ ID N° 207 CAAGCTGATGGAGGTTCATG GTGATTATTC protein S3a AGAAGATGTT GGTGTGAAGT TGGATCGGCCAGTTGATGAG ACAGTGGCAG AGGCAGAACC CGAGGTTCCT GGAGCGTAGA CTCGTTTCGTGCTTCCGAAA TATGTGTTCG AATATGGTGA TAGTCTTTAG AGCCTCACAT TGTTTA C426bGATCCCACCAGATCAGCAGAGGCTCATATTTGCTG ubiquitin SEQ ID N° 208GTAAGCAGCTGGAGGATGGGCGCACCCTTGCAGAT TACAATATCCAAAAGGAATCCACACTCCACCTTGTGCTTCGCCTTCGTGGTGGTGACTATTGAGGATTGAAGTGCTGCTGCTGGGGTTTTACATAAGATGCCTGCTT CTTTGTTCTAATGGTTCTGTTGTTA C428aGATCCTGATG TTACTGCCCG CCCTAAAGCT putative protein SEQ ID N° 209CTTGAGTGCA ATCTCATCTT TA At1g27760 [A. thaliana] C428b GATCCTCCAAGGAGATAGCT TTGGCATCTC putative protein SEQ ID N° 210 ATTTTCTTGGAATTTTGGCT TTA At3g09350 [A. thaliana] C429GATCCTGCTGGTTGGCTAGAATGGGATGGTAATTTT putative SEQ ID N° 211 GCTTTApectinesterase C430 GCTCATTACAATTTTGGACACATCAAGTGATCCAA vacuolar H(+)-SEQ ID N° 212 GGACACTTGCTGTTGCTTGCTATGATCTATCGCAGT ATPase subunit-likeTCATTCAATGCCATTCTGCTGGGCGAATTATAGTGA proteinATGACCTCAAAGCTAAGGAGCGCGTAATGAAACTG TTGAACCACGAAAATGCAGAGGTCACGAAAAATGCCTTACTCTGTATCCAAAGGCTTTTCCTAGGTGCAAAGTATGCTAGCTTTTTGCAGGTTTAGTTCTCATCGAA GGGTTTGATTGTTCAGACGATGAAAACTAGACATATCTTGTTATTTCATTGAAACAAAAGGAGTTTGATCG TGTTCGTGTTA C431a GATCCTGCACGTCTGCCTGC TTTTCATTGT monodehydro SEQ ID N° 213 TGTGTCGGTA CGAATGAGGAAAGGTTGACC ascorbate CCGAAGTGGT ACAAGGAACA TGGCATTGAA reductaseTTGGTCCTTG GAACTCGTGT AAAATCAGCT GACGTGAGAC GGAAGACACT GTTGACTGCAACTGGTGAGA CCATAACCTA CAAGATTCTC ATAGTGGCAA CTGGTGCTCG GGCTTTGAAGCTTGAAGAGT TTGGAGTGAG TGGATCAGAT GCTGATGGTG TATGTTATTT ACGAGATTTGGCTGATGCAA ACAGGCTGGT TA C431b GATCCTCTGAGGCTATTATGCTTGCTGGATTAGCTTglutamate SEQ ID N° 214 TCACGAGAAAATGGCAAAATAAAATGAAAGCCCA decarboxylaseAGGTAAGCCCTGTGACAAGCCCAATATTGTCACTG GTGCCAATGTCCAGGTGTGTTGGGAGAAATTTGCAAGGTATTTTGAAGTGGAGCTAAAGGAAGTAAAGTT GAGTGATGGATACTATGTGATGGACCCTGAGAAAGCTGTGGAAATGGTGGATGAGAACACAATTTGTGTA GCTGCTATCTTTGGGTT C432AAACCGGTGCGATTTGAAAATACTGCTGGCGATCT isoflavone synthase- SEQ ID N° 215TACAGGAAAATCACTATCAGGTCATTCCTTTCGGTT like proteinCAGCAACAAGAATGTGTCCAGGGAATGTCGATGGG TTGAGTTA C433bGATCCTGCTGTAATGGGAATTGGCCCAGCCGTTGC 3-ketoacyl-CoA SEQ ID N° 216GATACCAGCTGCTGTTA thiolase C434a TAAGCAGCGATGACCTCTTTGAAAGTGGAAGCTCAputative protein SEQ ID N° 217 AGTGATGATGCTGATGACGAGTTGACTGATAAAAGAT5g43720 [A. TGCAAGAGAACAAGCTTCTAGTACATCAGTGAAAG thaliana]CAGCTTTCTAGCATGTCCAGCGATGAAAAAAATCAG AGGCAAATATCCGCCCGTGCTCTAATGCCACCACCTCGTCCTTCGAGCAAGTCATTTAGTCATTCAGTAAA TAAAAAATCACGGTTTGGAGGATC C435bGATCCTCAAAATGGACTGTCAAGGAAGTTGCTGAA mutator transposase SEQ ID N° 218TGTGTTACTCAGGACTCATCAAGCGGGGAAATAAA AAAGAAGCAAAACAGATGCTCCATATGCAAAACGACTAGCCACAAAAGAACTACTTGCAAGAAGAGAAC TGAAGGAACAAGCAACTCCATTGTGGCTTA C436aTAAGGCATCA TATATACATC ATCTCGATGC porin SEQ ID N° 219 ATTGAAGAGGAGTGCTGCTG TGGGTGTAAT CACTAGAAGG TTCTCTTCAA ATGAGCACAC ATTTACAGTTGGAGGATCC C436b TAAGCATGGAAACCGCCTTTGTCCTATCTGCAGATG retroelement polSEQ ID N° 220 CAAATGGAAGGAAATCCCTCTCCAATTTCCCACCTT polyproteinCAGTACTGATGTAAACGGTATCAATAATCCCGCG C438GTTTAAGACATTTGATCTTAGCTACTTCAAGCTTTT peroxidase SEQ ID N° 221GCTCAAGAGGAGAGGTCTGTTCCAATCTGATGCAG CCTTA C439a GATCCTGAGA AAGCTGTAGAAATGGTGGAT glutamate SEQ ID N° 222 GAGAACACTA TTTGTGTAGC TGCTATCTTGdecarboxylase GGTTCCACCC TTACTCAGGG GTCATCAATC ACTAGT C439b GATCCTCCAAACCTGAAGAC CAATGCAGTC putative protein SEQ ID N° 223 GAACAACCAGAATGCAAGGG AGAGAAGGTT At4g09150 [A. GATCTGTTCT TA thaliana] C441aGATCCTCAGCAATTCTAATGGTTCACAAGGCCAGA Na+/H+ antiporter SEQ ID N° 224AAGAACGGGCTTCCCTTTTGGAATAAAGGACAAGT AGGGGAATCGAACCAAGTCATTGTAGCATTTGAGACATTCGGACAACTCAGTAAGGTGTCAATTCGACCA CAACTGCAATCTCCGCTATGACAAGTATGCACGAGGACATAATTGCTAGCGCGGAGAGAAAAAGAGTTT CAATGATAATTTTACCGTTCCATAAACATCAGAGAATTGGCGGACAATTTGAAACGACACGAGCTGATCT TAGACTTGTCAATCGAAGAGTTCTACAACACGCACCATGTTCTGTTAGCATATTA C441b TGATGTTGAT ATCGCGACTC ATATACATGT putativeprotein SEQ ID N° 225 CAAGGATGAT GGACCTAAAA GGAGTATACT At5g04740 [A.GCATGTTGAA ACTGCTGATC GATCTGGTTT thaliana] GCTGGTGGAA GTCGTCAAAATAATGGCTGA CATTAGCATT GATGCTGAAT CAGGAGAGAT TGATACAGAA GGTCTAGTTGCGAAGGGCAA GTTCTATGTC AGTTACAGAG GGGCAGCATT ACTCAGGACT CATCGATGAGTCCTGAGTAA CCACAAATGC CAAACCAAAA GAGCCAATAA ATTATACCTT ACATTGAACTGCCATTCTCA AAAAATGGCA CTANGAACTA ATACACACTG TTCGTTGATG GGGTAAAGCAAAAAAATAGG CAAATACTAG GGGAACCATA CAACATCAGC CTAGATACTA TGCAGTTAGTCAGGTTCCTC CATCCTTGTA CCCCCAGCAT CAGCTTCAGG ATC C442ATGTTGGACAACCTTTAGCTCAGTTACTTTATCACT cytochrome P450 SEQ ID N° 226TCGATTGGAAACTCCCTAATGGACAAACTCACCAA AATTTCGACATGACTGAGTCACCTGGAATTTCTGTTACATGAAAGGCTGATCTTATTATGATTGCCACTCCT GCTCATTCTTGATTA C443aGATCCTAGTTTGGAATATGAGCTCTCTGCTCTTCGA potassium SEQ ID N° 227GAACCCACAGAATCTGGATTTACATATTTGCTTGG transporterCATGGGGACGTGAGGGCGAAGAAAAACTCTTGGTT CATCAAGAAACTGTCAATAAATTACTTCTATGCATTCATGAGGAAGAACTGTAGAGGAGGCGCTGCAACA ATGCGTGTTCCTCACATGAATATTATCCAGGTGGGATGACATACATGGTTTGATCTTGGTACCATTTAGCT TCTTGCTGGCCTTGTAAGTGCTGCATTA C443bGATCCATGCA GATATTCCAT GGGGCGATTT glyceraldehyde-3- SEQ ID N° 228AGGTGCAGAT TATGTTGTTG AATCTTCTGG phosphate TGTTTTCACA ACCGTTGAGAAGGCTTCAGC dehydrogenase ACATAAGAAG GGTGGTGCAA AAAAGGTCGT AATCTCAGCTCCATCAGCTG ATGCACCTAT GTTTGTGGTA GGAGTGAATG AGAGAACTTT CAAAACCACCATGGATGTTG TTTATAATGC TAGCTGTAGT ACCAATTGCC TTGCTCCCCT TGCCAAGGTGGTTCATGAGG AGTTTGGCAT TGTTGAAGGA TTA C444 GATCCTCAAG CATTTATTTGCCACTTTTAC heme oxygenase 1 SEQ ID N° 229 AACACATACT TTGCGCATTCAGCTGGAGGT CGCATGATAG GGAGAAAGGT GGCTGAAAAG ATACTCAATA AGAAAGAGCTGGAATTCTAC AAATGGGACG GTGACCTTTC TCAGCTGCTG CAGAATGTTA GAGAGAAGCTGAATAAAGTT GCAGAAAACT GGACTAGAGA GGAGAAGAAT CATTGTTTGG AAGAGACGGGGAAGTCATTTC AAGTTCTCAG GGGAAATCCT CCGATTA C445 GATCCTCTCA TCATTGTCCAGGAGGTCTGT putative inorganic SEQ ID N° 230 TGCTGCTCAC CCTTGGCACGATCTTGAGAT pyrophosphatase TGGACCTGAA GCTCCAAAGG TTTTCAATGT TGTCATTGAGATTACAAAAG GTAGTAAAGT CAAATACGAG CTTGACAAGA AAACTGGTCT CATTA C446aTAATGGAAGA TGCACCACTG GAATGAGCAA cytochrome c SEQ ID N° 231 AGAAAAGTTAGGTCATTTTA TGACTTGCTG oxidase subunit 5c GAGAAAGGTG AAATAAGTTTAGTCGCAGAA GAATAATTTT TCGAGGATC C446bTAATGGATGATACTGCTGAGGCAAAAGCTTGTCAA putative protein SEQ ID N° 232GACGAAGTGAATGCTATTCTGGGAGAGAAGCTATC At5g09260 [A.TGCTGATTATGAAGAGGAAGTTTTAGCACAATTTG thaliana] AGGATC C447GATCCTCATGACATATGTGAACAACATCCTGACAT arogenate SEQ ID N° 233CGTCGTACTCTGCACTTTCCATTA dehydrogenase C448 GATCCTGGTC GCCTGACAGGCAAGAGAGAT catalase 3 SEQ ID N° 234 TTATCTGCAG ATGGATTA C449a GATCCTGCTGTTTTTACTGG GGATACATTG glyoxalase II SEQ ID N° 235 TTTATTGCTG GTTGTGGTAAGTTTTTTGAA GGCAGTGCAG AACAAATGTA TCAGTCACTG TGTGTGACAC TAGGTTTCTTGTCAAAGCCA ACTCGGGTGT ATTGTGGCCA TGAGTACACA GTAAAAAATT TGCAGTTTGC TTTAC449b GATCCTGAGG GTGCTCATTA CAATTTTGGA vacuolar H(+)- SEQ ID N° 236CACATCAAGT GATCCAAGGA CACTTGCTGT ATPase GCTTTGCTAT GATCTATCGC AGTTCATTCAATGCCATTCT GCTGGGCGAA TTATAGTGAA TGACCTCAAA GCTAAGGAGC GCGTAATGAAACTGTTGAAC CACGAAAATG CAGAGGTCAC GAAAAATGCC TTACTCTGTA TCCAAAGGCTTTTCCTAGGT GCAAAGTATG CTAGCTLTTT GCAGGTTTAG TTCTCATCGA AGGGTTTGATTGTTCAGACG ATGAAAACTG GACATATCTT GTTATTTCAT TGAAACAAAA GGAGTTTGATCGTGTTCGTG TTA C449c TGACTGCGTAGTGCTCCTGACGGTTATTGGATCGAG glyoxalase ISEQ ID N° 237 ATTTTTGGCACTAAACCTATCAAAGAAGTTGCTGATGCTGCTTCTTGATTCAGGGGCTCTTCGAGTGTCTAT CACGAGTGTTGATCAACTCAGCTATCTGTTGAAGAGAGAGTTTCTCGTAAACAGCGTTTTCTTTCCAGGTTA C450 GATCCTGGTG TTAGCAACAATGAAGATGAG putative protein 66b SEQ ID N° 238 GATGTTGAGG ATATCAATGTTGCAGAGGAC [Daucus carota] GATATGATGG ATGATGTGCT TGACGTGGAT GATAATAACCAGAGGAGTGA TGAAATTGTA AAAGTTGAAG CCGGTAATGG TAGTACACAG ATTGATCAGCAGAAGATATG CATCTCTTAT CTCTATTAAA GGTTTAGTTT GTGTTTA C451a GATCCTGCTGTAATGCCAAT TGGCCCAGCC 3-ketoacyl-CoA SEQ ID N° 239 GTTGCGATAC CAGCTGGTGTTA thiolase C452a GATCCTGATAGAACTGAATCCGAGGATTCTGATGA putative SRprotein SEQ ID N° 240 TTCAATATAGCCGAGGACATTTTTCAGCAGACAATGATTAGTTAGCTACAAAAGCTGTTTTTGGCAAGTG GTTACCAAGTCTCCGCCATTGATATAGTTACTTCATGGTTA C452b GATCCTGTTT GTGGAAGTGC CCATTGTGCT PHZF-like protein SEQ ID N°241 TTGGCTCCTT ATTGGCATAA AAAGCTTGGC AAATGTGACT TTGTTGCTTT AGCGGCCTCAACTAGAGGTG GCGTTGTGAA CGTGCATCTA GACGAGGAGA ATCAGAGGGT ACTTCTGAGAGGGAAAGCTG TTGTTGTTAT GGAAGGTACT CTTCTAGTTT A C452c GATCCTGAACTTCCCCCTGA AATGAGAGAA mitochondrial SEQ ID N° 242 GCTCATCGTT ACAAGCTTTCAAAATTGCCA ribosomal protein AGGAACAGTT CTTTTACCCG AATCAGAAAT S14CGGTGCGTTT TCACTGGTCG GCCACGTGCT GTGTATGAGA AGTTTAGAAT GTCGCGTATTGTGTTCCGTG GTTTGGCTGC TCGCGGTGCT TTGCAAGGTG TTTA C453TTTCATACCATGGCGATTTGAAAATACATCTGTTTGA cytochrome P450 SEQ ID N° 243TCTTACGGGAAATCACTATCAGTTCATTCCTTTTGG TTCAGGAAGAAGAATGTGTCCTGGAATGTCGTTTGGTTTA C454 ACAGCTATGA CCATTAAGCC TATTTAGGTG putative SEQ ID N° 244ACACTATAGA ACAAGTTTGT ACAAAAAAGC phosphatase 2C AGGCTGGTAC CGGTCCGGAATTCCCGGGAT CTCTCAGTTT TTTTCATCCA TTCCTCTTCA GCCAATCCCA AGAGGGTCATCATTTGCAGC TTCTACTATT CATTCAGGCC CTATCCCGGC CCGTATTTCT AGTACGTACCCTTGCTCGGG CCCGATCGAG AGGGGATTCA TGTCCGGCCC GATTGAGCGG AGCTTCACCTCGGGCCCGTT GGAGAACCAG TATGATCATA TCCAAAGGTA CAAGCCCAAG TCCAAGAAATGGGGTTTAAT TAAAAGTTTA AAGAAAGTGT TGTCAAATTC CTTTTTGGGG TTTAATAAAGAAATGAATTT GGTAGAGAAG AATAATAATA ATGAAGTTAA TGTTCAAGGG AGTAATAGTCATCATAGTAA TGTTGGAAAT AGTTTGAGTA GTCAGAATAG TTTGGTTGAT GATGATGATGAGGGAAATGA CTCATTTAGA GGCCAAAATG TGCAATGGGC TCAAGGTAAA GCAGGGGAAGACAGAGTACA TGTTGTGATT TCTGAGGAAC ATGGTTGGGT TTTTGTAGGG ATATATGATGGATTTAATGG ACCTGATGCT ACTGATTTTC TGTTAAACAA TCTTTATTCA AATGTCTATAAAGAACTCAA GGGATTGCTA TGGAATGATA AGTTAAAAAC CCCCAAGAAT TCGACGAGCAACGAGACTGT TCCGTTAAGA AACTCGGGTT TTAAGGTGGA ACATTTTGTT CAAAATCAAGAATTAGATCA GAGGGAGAAA CTTGATGGGG TTGTTGGTGT TGACCATTCT GATGTATTGAAGGCTTTATC TGAAGGGTTG AGGAAAACCG AGGCGTCGTA TTTGGAGATT GCTGATATGATGGTAAAGGA GAATCCTGAA TTGGCTTTAA TGGGATCTTG TGTTTTAGTA ATGTTGCTTAAAGATCAGGA TGTTTATTTG TTGAATGTTG GAGATAGTAG AGCTGTTTTA GCTCAAAATCCTGAGTCTGA TATTTCTATT AGCAAATTGA AAAGGATAAA TGAGCAGAGT GTAAATAGCATTGATGCACT CTATCGAGCT GAATCTGATC GCAAACATAA TCTAATTCCT TCTCAACTTACTATGGATCA TAGCACATCT ATTAAAGAGG AAGTAATTAG GATTAGAAGT GAGCATTTGGATGATCCTTT CGCGATTAAA AATGATAGAG TGAAAGGTTC CTTGAAAGTT ACTCGAGCTTTCGGGGCAGG ATATCTCAAA CAGCCCAAGT GGAATAATGC ACTTCTAGAG ATGTTCAGAATTAACTACAT TGGGAATTCG CCTTACATCA ACTGTTTACC ATCGCTTTAC CACCACACTCTTGGTTCGAG AGACAGATTT TTGATCTTAT CATCTGATGG TCTTTACCAA TACTTCACAAATGAAGAAGC AGTCTCAGAA GTAGAGACCT TTATGTCTAT ATTCCCCGAG GGAGATCCTGCACAACATCT CGTCGAAGAA GTGTTATTCA GAGCTGCTAA GAAAGCTGGA TTGAACTTCCATGAGTTGCT CGATATACCT CAAGGAGATC GTAGGAAGTA CCATGATGAT GTTTCAATTATCATTTTGTC CTTCGAAGGA AGGATATGGA AATCATCGTT GTAAATCAGC TAGACACAGGAATTTTTATA TTTTACCCTC AGAAATCAGG AAAAAAAGAA AGTACATAGA AAAAATCGAGCTAATTTTGC TGTTAACCGT TGTTTACCCA ATTTTAGCAG TAGTGTTTAT AGTATACAGTCTAGGCTGCT CGATAAAAGA TAGCGAGGCT GAGGTTTCTT GATCCAGAGA TTGTAAAATTGCCAATAAAC TTATAACAAC CCCTGCCTCT TCTACATTCA AATGTTATTA GGACATGGTAAGTTTTGTAA CAGATGGTGC TCCTTGTATA CATTCTGGAG TTCCATTTCA CAAAAAAAAAAAAAAAAAAA AAAAAAAAAA AAAA C456 AAACCGGTTGCGATTGGAAAATACTTCTGGTGCTCTisoflavone synthase- SEQ ID N° 245 TACAGGAAAATCACTATCAGGTCATTTCCTTTCGGTTlike protein CAGGAAGAAGAATGTGTCCAGGGAATGTCGTTGGG TTTAGTTA C457TCGGGTATTG AAGCACAAGA ATGGGAAGTT acetyl Co-A SEQ ID N° 246 GGGTGTTGCAGGAATCTGCA ATGGGGGAGG acetyltransferase AGGCGCATCT GCTCTTGTTG TAGAGCTCATGCCTATAAGG ATGGTGGCAC GTTCATCGCT TTGAAACTGG AATAGTTTGT ACTATATTTACGTCTAGCTG CTGCACAGTT GCATGCCTGC TGAGTTCTGC CACATTGCGT CAAAAGTAGTGAGGTATCTG AATGCTTGTA TCCATTATGT AAAACCATAT AAGCAATAAC CTAATAATACCATGAAAATC GAGCAAACAC TTGTTTCCCT TA C458a GATCCTGGAG AATACTGGAGAGCTGTGATG specific tissue SEQ ID N° 247 AACGATGAGC CAATGCCTGAAGCAATCAAA protein CATCTTATGC CTCAGCATTC TGTTCCTCTC TCCATAGAGAAAACTGATTG TTACACATTA CCTTCTACTG GAGGTGAAGC CTTTGAACCA AGGCCTAATCTATCTGTCTA CCACGATGAC GCCAAGCTGA AAGAAGCTGA GAAATTATTA TTTATGAAAGATTTTGAGCC AAGGCCTACT ATAACTGGTT ATCATAATAA TGATGCTGGT CTTA C458bGATCCTGTAA TGAAGGAAGA AATTGACAGG SKP1-like protein ? SEQ ID N° 248GAGGTTGAGG ATTTTGCTAG GAGACTGAAC TCTGTTTGGC CAGAAAGAAT GCAGGAGATTTTGTCTTTGG GTCAAGAGAG GAGGCCTGTA CCACTATCTG TGAATGGGAA TGGTTCCCTAAAGAGATATA CGGGTTTGGA TGGGAGATAA TGGTTCAAAT GGTGGATGAT GAATCTTTTGGCTTCAGTCG AGCTTACTCA GGACTCATCA TCACTGGTTT TGTTATTACA TAGTGTGTTT GCTTAC461 GATCCTGATCCTAGACATTATTTACCTCTTTACCTT gene feebly protein SEQ ID N°249 AGACCAGCAACCTGATATGTTTTATAGGATGTGCACTTTGTAACCTTTGTATGAGATGAATATGTAACATG GTGTACGTAAAGTTTGAAAGTATAATATGTAAGATCACGTAAATCTATAGGTAAGGCTTA C462 GATCCTGGTAGTTTCAAGACATTTGATCTTAGCTACputative peroxidase SEQ ID N° 250 TTCACAGCTTTTGCTCAAGAGGAGAGGTCTGTTCCAATCTGATGCAGCCTTA C463a GATCCTGAGA AAGCTGTAGA AATGGTGGAT glutamate SEQ IDN° 251 GAGAACACTA TTTGTGTAGC TGCTATCTTG decarboxylase GGTTCCACCC TTAC463c GATCCTGGAT GCAGGCGGGT TTTTATCTAG ADP-ribosylation SEQ ID N° 252TTATTTTTTT CTTCTCAAGT CAGTGTGGTT factor ATGAACATCT CCTTTA C464GATCCTGATAAACCAACATTATCGTAGAGAATGTTT histamine-releasing SEQ ID N° 253TCTCTGTTTCTCCCTCTGAAGAACTTGCTTA factor homolog C465TAATCCAAAGTAGCAGATAATATCATAAATGCGCG putative protein SEQ ID N° 254GAAGAACAACCCAACACAGCTCGATACCAGGGTGT kinaseCACTAGTCAAGAGCATCTATAAAACATAATACAAG TCTGAAGAGTCTATAACTATTACAAATGTCTGATACAAGATAGAAATGATAAAGAGGGAGAAACACATGA CTACGGACATCAAACAACTACCTCGTGGTCTCTAAATGTGCTAGGAGCTCTCAACTTACACTTGCAGGATC C466GAAGCTGGGCACAATGAGCCTAGCTTGGTAGCAAG putative SEQ ID N° 255ACTTGTGAACTTACTCAGATACTATGCTGCTGGGCT transcription factorCGATTCTATTGGTTTCAGCCTTCCACCATACAGCCC SCARECROW TTGCAGGATTA C467ATCCTGTAG AGAAGGGATA TGTGGGTCCT succinate SEQ ID N° 256 GTGCTATGAATATTGATGGT TGCAATGGAC dehydrogenase iron- TTGCTTGTTT GACTAAGATCGATTCGGGTG protein subunit CTGAATCGAC GACTACGCCG TTGCCACATA TGTTTGTGATTA C469a GATCCTCTAC ATGAAAATGC AAATTTCATG putative protein SEQ ID N° 257AATGTGAAAT GGTATACTTT GCTTCGTAAG AT5g08550 [A. TATGGACTCT CTACAGATGAAAATCCAAAT thaliana] AGCTTTGATG ACGCTGATGC CAATCCTGTT CAATTGGTGGTGAAACTTGC AATGGCCATT CTACATAACC GGTTAGCTCA GTGCTGGGAT GTGTTTAGCACCCGTGAGAC ACAGTGTGCT GTATCTGCCA TAAATCTGTT GTTA C469b GATCCCAAGAGACTGGTTGA ATACTACAAA putative glutathione SEQ ID N° 258 AACCGTTTTATGGCCTAGAA TTTCAAAACG S-transferase GTTTGTCAAC CATTGGTGAA ACTGCGAATGAAGCACGCGC TGTATAAGTA TGTCATGGAG TTCTACAGAA TTGTTGATTA GTAATAGATAAATAAATTGG TCATGTCCTT TTTTTTATCT GTAGAATTGT GAATTATTTT TGGGGTTTGGTGTTTATGCT AGGGACTTGG ATTA C471a GATCCTTTTCTGAAAAAATTCTTTTTCCAACGGTTTAChexose transporter SEQ ID N° 259 AAGAGAACAAAGGATCAAGGATTGAACAGTAATTACTGCAAGTATGATAATCAAGGGCTGCAGCTATT ACTTTCATCTTTATATCTGGCCGGTTTA C471bGATCCTTACAGGTGGTTCAGTCATAGAATCTGAGG aldehyde SEQ ID N° 260GTAACTTTGTGCATCCAACAATTGTTGAAATATCTT dehydrogenaseCAAAAGCTGAAGTTTGTGAAGGAAGAATTGTTTGCT CCAGTTCTTTATGTAATGAAGTTTA C472GATCCTTCAC TGTGTAATCA AACAAAAAGA quinolinate SEQ ID N° 261 TGTAAATTGCTGGAATATCT CAGATGGCTC phosphoribosyltrans TTTTCCAACC TTATTGCTTGAGTTGGTAAT ferase TTCATTATAG CTTTGTTTTC ATGTTTA C474TGCGTAATCAAACAAAAAGATGTAAATTGCTGGAA quinolinate SEQ ID N° 262TATCTCAGATGGCTCTTTTCCAACCTTATTGCTTGA phosphoribosyltransGTTGGTAATTTCATTATAGCTTTGTTTTCATGTTTCA feraseTGGAATTTGTTACAATGAAAATACTTGATTTATAAG TTTGGTGTATGTAAAATTCTGTGTTA C475TAACGTTGGTTCTCCAAGGGGAATTTCAGGCGAGC putative lipid SEQ ID N° 263GAGGCAGTGACATGCAGTGCCTCGCAGCTAAGTGA transfer proteinGTGTGTGGGGGCGGTGACGTCGTCACAGGCACCAT CTTCGGCATGTTGCAGCAAAATGAGGGACCAACAGCCTTGTCTGTGTGGGTACATGAAGGATC C476a TGTCTGGATC AAACCTTGCT GCCCCATATC MAPkinase SEQ ID N° 264 CTCTCTCCTT CCTAACATGG TGGGGTGGCT ATGTCTGTCCCCACTATTCC CACGTGCTTT CTCCTCCCCA CTTATATAAA CACAAATTTC ACTGAAGAGGAGAAGAATCC ATTTCCATTC CAACAAATCC AAACGGACCC GACCCGATTC ACCCCACCACATGGCCTTAG TCCGAGAACG TCGACAGCTC AATCTCAGAC TTCCCTTGCC GGAACCCTCCGAACGCCGCC CTCGTTTCCC CTTACCCCTC CCTCCTTCCA TCTCCACCAC CACAACTGCTCCTACCACTA CTATCTCCAT CTCGGAACTC GAAAAGCTTA AGGTTCTCGG TCACGGAAACGGCGGAACTG TGTACAAAGT CCGCCACAAA CGCACATCCG CAATCTACGC TCTCAAAGTCGTTCACGGCG ATAGCGACCC CGAGATTCGC CGTCAAATCC TCCGTGAAAT CTCCATCCTTCGCCGGACGG ATTCTCCTTA CGTCATCAAG TGCCACGGTG TCATCGACAT GCCCGGCGGCGACATCGGTA TCCTTATGGA GTACATGAAC GTCGGCACAC TAGAAAGTCT TTTAAAATCACAAGCAACTT TCTCCGAACT TAGCTTAGCA AAAATCGCTA AGCAAGTACT TAGCGGACTCGACTACTTAC ACAATCACAA AATCATTCAC AGAGATTTAA AACCTTCGAA CCTTCTAGTAAATCGCGAGA TGGAAGTAAA AATCGCCGAT TTCGGAGTGA GTAAAATCAT GTGCAGGACTTTAGATCCTT GCAATTCATA CGTTGGAACT TGTGCTTATA TGAGCCCAGC AAGGTTTGATCCAGACACTT ATGGAGTTAA CTACAACGGT TACGCAGCTG ATATTTGGAG TTTGGGCTTGACTTTAATGG AACTATATAT GGGCCACTTT CCGTTCTTGC CACCTGGACA GAGACCGGACTGGGCTACGC TAATGTGCGC CATATGCTTC GGTGAGCCGC CCAGTTTGCC TGAAGGGACGTCGGGAAATT TCAGAGATTT TATCGAGTGT TGTTTACAGA AAGAGTCCAG TAAAAGGTGGAGCGCTCAGC AACTTTTGCA ACATCCGTTT ATACTGAGCA TCGATTTGAA GTCCACGTAAAAAGGGACAG AGCAAAGCTG AAGACTGGGA AATTGAATAG TTCCGAGTTG TTTGTAAATAGAGAACGGGA CCTTCTTTTT TTTTTTGAAC TTTTTGGGTT AACTTTTTTG TATATTCTTCAACTATGAAT CTGTGAAATC AGAATCATTC TCTGTATCTG GAAAAAGTGC CCATTTTCCATAGCAAAAAA AATCATCTGT GGAATTTTGA GACTTAATGA ATTCAATCTT TTTCCAACAAAAAAAAAAAA C476b GATCCTCGTG AGGTTGCTGC TGCTAAAGCA succinyl-CoA ligaseSEQ ID N° 265 GATTTGAATT ATATTGGCTT GGATGGAGAA ATTGGTTGCA TGGTTA C477CCAGCTATGA CCATTAGTGC CTATTTAGGT putative zinc SEQ ID N° 266 GACACTATAGAACAAGTTTG TACAAAAAAG transporter CAGGCTGGTA CCGGTCCGGA ATTCCCGGGATTTTTTCTAT TCCGTGATCC CCTTTATCTC TTCCCCTTTT TCTCCTTTTT CTTCTTCGTTTAGGTATATA CCCCATATAT ATAGCCTATA AACCATATAG CTATATAAAA CTCTACATCTATTTTGAGAA TTTGATGATT TGGGTCGGCT AAAAATACAA TCTTTTTAAT ACTCTTTTGAAATCTTGGCA CAAATTTGTG AGATGGAGAC GCAGAACCTG GAACGTGGAC ATGTAATTGAGGTACGTTGT GACATGGCAG CTCAAGAAAA GGGGACTAAA ATCTGTGGTT CAGCACCGTGTGGATTCTCA GATGTTAACA CCATGTCTAA GGATGCACAG GAGAGATCAG CATCCATGAGGAAACTTTGC ATCGCGGTTG TCCTCTGCAT CATATTTATG GCTGTTGAGG TTGTTGGTGGTATTAAAGCC AACAGTCTGG CAATATTGAC CGACGCTGCT CATCTACTAT CAGATGTTGCAGCTTTTGCA ATATCCTTGT TTTCACTCTG GGCAGCAGGA TGGGAAGATA ATCCACGCCAGTCCTATGGG TTTTTCAGAA TCGAGATACT CGGGGCATTA GTTTCTATCC AAATGATATGGATTCTAGCT GGGATCCTTG TTTATGAAGC CATTGCTCGA CTTATTCATG ATACAGGTGAAGTTCAAGGC TTCCTCATGT TTGTGGTGTC TGCATTTGGA TTAGTAGTGA ACCTCATCATGGCACTCTTG TTAGGTCATG ATCATGGCCA CGGCCACGGC CATGGCCACA GCCACGGTCATGACCATGAA CACGGCCATA ATCATGGCGA GCATGCTCAT AGCAATACTG ATCATGAGCACGGCCATGGT GAGCATACGC ATATACATGG AATTAGCGTT AGCCGACACC ATCACCATAATGAGGGACCT TCGAGCCGAG ATCAACACTC GCACGCACAT GATGGAGATC ACACCGTGCCTCTACTTAAG AATTCATGTG AGGGTGAAAG TGTATCAGAA GGTGAAAAGA AAAAGAAGCCCCAGAACATA AATGTTCAGG GAGCTTATCT TCATGTAATC GGAGATTCTA TTCACAGCATAGGGGTGATG ATTGGGGGAG CTATTATATG GTATAAACCA GAGTGGAAAA TCATCGATCTAATTTGCACT CTCATTTTCT CTGTAATTGT GCTCGGGACA ACCATTAGGA TGCTTCGGAGTATTCTTGAA GTATTAATGG AGAGTACGCC CAGAGAAATT GATGCAACAA GGCTCCAGAAGGGGCTCTGT GAGATGGAGG ACGTTGTCCC AATCCATGAA TTGCACATAT GGGCAATTACAGTCGGCAAA GTGCTCCTGG CTTGCCATGT CAAGATTAAG TCCGACGCTG ATGCTGACACGGTGCTGGAT AAGGTGAT C478 ATATGTTACAGGGTCCATGCAGAGCGCTATTTGGCT sucrosetransport SEQ ID N° 267 GATCTGTCCGGCGGAAAAGCCGGGAGGATGAGAA proteinCATCAAAGGCCTTCTTCTCCTTCTTCATGGCCGTCGGAAACGTCCTCGGTTACGCCGCCGGTTCCTACTCCCGCCTCTACAAAATCTTCCCCTTCTCTAAAACCCCAGCCTGTGACATCTACTGCGCCAACCTCAAATCATGTT TCTTCATCGCCGTCTTCCTTCTACTCAGCTTAC479 TGTGTAATCAAACAAAAAGATGTAAATTGCTGGAA putative protein SEQ ID N° 268TATCTAGATGGCTCTTTTCCAACCTTATTGCTTGAG AAK58573TTGGTAATTTCATTATAGCTTTGTTTTCATGTTTCAT [Acidianus sp.]GGAATTTGTTACAATGAAAATACTTGATTTATAAGTTTGGTGTATGTAAAATTCTGTGTTACTTCAAATATT TTGAGATGTTGAATATCATGTTCTTA C480TCCAAGAGTCTACCACGAGCTAATTCCGAATGTAG gamma- SEQ ID N° 269TTCTGTACGAGAACTGGACGTGCATCGATGGCGAT glutamyltransferase-CATATTGAACTCTCGGACGAGAAAAAGGCATTTCT like proteinTGGAAGAGAGGGGTCATCAACTCGAGGCACATAAC GGAGGAGCCATCTGTCAGCTAATTGTTCAAAACCTTCCAAATTCTCCCTTA C481 GATCCTTCAC TGTGTAATCA AACAAAAAGA quinolinate SEQID N° 270 TGTAAATTGC TGGAATATCT CAGATGGCTC phosphoribosyltransTTTTCCAACC TTA ferase C482 GATCCTTGGC AGACAAACAG GGTCGAAAGC putativeprotein SEQ ID N° 271 GGGCTTGTGT CACGTACTGC ATCACTTACA AT4g27720 [A.TTTTGAGCTG TATGACCAAA CATTCTCCTC thaliana] AGTACAAAAT TTTGATGTTGGGCCGTATAT TAGGAGGAAT TGCCACCTCT CTCCTATTCT CAGCCTTTGA ATCTTGGCTTGTTGCAGAGC ATAATAAGAG GGGTTTTGAT CAACAATGGC TATCATTA C483a GATCCTTTGGGCAAAGGTCG AGATGGAACT receptor-like protein SEQ ID N° 272 GCTTTCTCTCAGGAAGTATT TGAGAGCTTT kinase ATGTTCAATT TGGATGAAGT TGAGTCTGCT ACACAGTATTTTTCAGAGGC AAATTTGTTA GGGAAGAGTA ATTTCACAGC CGTTTATAAA GGGACACTGAGGGATGGGTC TTCTGTTGCT ATTA C483b GATCCTTTAC AAACAGAGTA GAAAGATGCAmutator-like SEQ ID N° 273 GTGAGACATG AATTACATTG ATTTTGGTTT transposaseTGGCATTCTT TTCTCGCAAG ATATGTTGTA AGCATAGTAT CAGTAGGTCA TTATTCCGATTTTCCCCTCA ATTGGGGAAA GGGAGGAGGT GTGTGACCTT GGTCACGGTT GTACCATTA C483cGATCCTTGGGCCCGATGTCCATGAGGTGGATTACG delta-1-pyrroline-5- SEQ ID N° 274TTGCATGGGTTTGTGATCAAGATGCATATGCATGTA carboxylateGTGGTCAGAAGTGTTCAGCTCAATCAATATTGTTCA dehydrogenaseTGCATGAGAATTGGGGTAGAAGCTCTCTCTTAGAC AAAATGACCGAGCTTGCTGCAAGAAGAAAGTTGGATGATTA C484a AAAACATCAT GAATAACACC ACCTTTTCCG C3HC4-type RING SEQ ID N°275 TCCAAATTTC CGACACCGGA GGTTTCCTCG zinc finger protein GATCGGGAAAAATCGGAGGA TTCGGCTACG GAATTGGTGT TTCAGTAGGT ATTCTTATTT TAATTACAACAATAACCCTC ACTTCCTATT TTTGTACTCG AAATCAAACA TCAGAGTTAC CAACAAGAAGACAAAGAACA ATTAATCGAA ACGAGCTTTC TGGACATTGT GTGGTTGATA TTGGGCTCGATGAAAAAACC CTTTTGAGTT ATCCCAAGTT GTTGTACTCT GAAGCTAAGG TCAATCATAAGGACTCAACA GCTAGTTGTT GTTCCATATG TTTAGGAGAT TACAAGAAAA AAGACATGCTTCGATTGTTG CCAGATTGTG GACATTTGTT TGACTTGAAA TGTGTGGATG CTTGGCTCATGTTGAATCCA AGTTGTCCAG TTTGTAGAAC ATCTCCATTG CCAACACCAC AATCTACTCCTTTGGCTGAG GTTGTTCCTT TGGCAACTAG ACCTTTGGGA TGA C484b GATCCTTGTGCCCCTTCCGG AGCCAGAAGC katanin SEQ ID N° 276 AAGGTGCGCC ATGTTTGAAGAATTACTACC ATCACTGCCT GAAGAGGAGT CACTTCCATA TGATTTATTG GTAGAAAAGACAGAAGGTTT TTCCGGTTCT GATATTCGGT TGTTGTGCAA GGAGGCTGCC ATGCAACCAT TAC485 CTTGGTAGTGCGCTTGGGCTGTTCGGTGTTATTGTG putative vacuolar SEQ ID N°277 GGAATTATTATGTCAGCTCAAGCATCTTGGCCATCC ATP synthaseAAGGGTGCGTAAGGCTTCATATTATGTGCTTGCTAT proteolipid subunitTGCTCCGGACTCATCA C5 GATCCCAAAA ATAAGTACCA ACTTCTTTGC ambiguous hit SEQID N° 278 TATGGTTTTT TGTGGAGAAC ATTTCACATC TTTTTCCCTG GGGATATATACTGTCCTGTC ATTGAATCTA ACAATGTCTT CTTCAACTTT CTTGGCCGCT CACTCCCCTCTGCTCAGCCT CCCCCACAAC CTTCTAAGAA AACAAACAAA ACACAAAATA CTCAATCAGCAGGTGGTTTA C6 GATCCCAAAG AAAGAATGCC AATTTCGGAT transposase-like SEQ IDN° 279 TACGGTCCTA ATATTCGAGA CGAAGTAAGG protein AGATATTATA TAAACAAAGGGCCTTGTCAA CCGATTGGTC ATGCGTTTCC TAAAACTAAG ATTGGGAGTA AAATGCGTCCATTTAGTCCC ACTTGGTTTA C7 GATCCCATCG ATTATTTGGT TTTCCGGTGA putativeprotein SEQ ID N° 280 GGATTCAATC CATCGAGGTT CCATCGTGGT AT5g44010 [A.CTCCGGCTTA CGGTCTATTT GTGTTCAACT thaliana] ATAGTGTCGC ATTTTTCTTGTAAACTAGTT GGAATATCTT TA C8a GATCCCAATT TTTCAGAATT GCTACTCTCAphosphate/phospho SEQ ID N° 281 GTATTGTCTT TTGTGGGTCT GTTGTGGGTGenolpyruvate GCAATATTTC TTTA translocator-like protein C8c GATCCCATTATATCCTACCG CAATTTTTCA putative protein SEQ ID N° 282 GGGTGAAATTGATGGTGAAG GGATGAGTTT At1g10410 [A. TGTCTTGTAC TTTA thaliana] C9GTGCTGTTCC AAGTAATGCC TCTGACAATG pyrophosphate- SEQ ID N° 283 TATATTGCACGCTTCTTGCT CAAAGTTGTG dependent TTCATGGAGC AATGGCAGGG TCCACAGGTTphosphofructo-1- ACACCTCGGG GCTTGTCAAC GGTCGCCAGA kinase-like proteinCTTATATTCC ATTCAATCGT ATAACCGAGA AGCAAAATAT GGTGGTTATA ACTGACAGGATGTGGGCACG TCTTCTTTCG TCAACCAATC AGCCAAGCTT CTTGTGCCCG AAAGATGCTTGAAGAGGTTA MAP2 ACAGCTATGA CCATTAGGAC CTATTTAGGT putative protein [A.SEQ ID N° 284 GACACTATAG AACAAGTTTG TACAAAAAAG thaliana] CAGGCTGGTACCGGTCCGGA ATTCCCGGGA TGTTACTTGA CGTGTTTTCT TTTCTTTTAC TCTCCGCCAATTCAAGACTT CTCAAAGTAC TTTCTCATCT AAAGCAAAAT GTCCGACGGA GGATTAACGGTTTTGGACGG ATCACAGCTG AGAGCCGTCA GCCTATCGTT ACCGTCATCG GACGGCAGCTCAGTCACCGG AGCTCAGCTT CTCGATTTCG CTGAATCCAA AGTCTCAGAG TCGCTCTTCGGCTTCTCATT GCCGGATACT CTCAAGTCCG CCGCTCTCAA ACGCCTCAGC GTCGCCGATGACCTTAATTT CCGCCGTGAA CAGCTCGATC GTGAAAATGC CTCGATCATT CTCCGAAATTACGTCGCTGC CATTGCAGAC GAACTCCAAG ATGATCCTAT AGTCATTGCA ATTTTGGATGGGAAAACTCT TTGTATGTTT TTGGAAGATG AAGACGACTT TGCCATGTTG GCTGAGAATCTTTTCACTGA TTTAGACACA GAAGATAGAG GAAAGATCAG AAGAAATCAA ATACGGGATGCTCTCATTCA TATGGGTGTT GAAATGGGAA TTCCTCCTCT TTCAGAGTTT CCTATACTAAGTGACATTTT AAAGAGGCAT GGAGCTGAAG GAGAGGACGA ACTGGGGCAA GCCCAATTTGCACATTTACT TCAGCCTGTG CTTCAGGAGC TGGCAGATGC TCTTGCTAAG AACCCTGTGGTTGTAGTGCA GAAAATCAAG ATCAATAATG GTTCCAAATT AAGAAAGGTT TTGGCTGATGAAAAGCAACT AAGTGAGACA GTAGAGAAGA TAATGCAGGA AAAGCAGGAT GAGAAGGATAGTCTAAGTAA CAAAGATGCC ATTCGGTGTT ATCTCGAGAA AAATGGAGCA TCATTGGGCTTGCCACCTCT GAAGAATGAT GAAGTGGTGA TTCTTCTATA CGACATTGTA TTAGGTGATATAGAAAATGG AAAGACCGAT GCAGCATCAG ATAAGGATGA AATCTTGGTT TTCCTGAAGGATATCCTTGA GAAATTTGCA GCTCAACTTG AAGTTAACCC AACTTTCCAT GATTTTGACAATTGAAGTTA TATACACCCT CTCAAGATAA GTTATACCAG AAAGATCATA TATATGTATTTTAGCCTTTG CTTTTGGTGC CAAGGCAACT TATAGTGTTT AATTTTTATA TTGTAGAATAACAAGTATTC ATGAGACAGA TAAATCAAAC CCATTTCATT TGCATTTCAA AAAAAAAAAAGGGCGGCCGC TCTAGAGTAT CCCTCGGGGG GCCCAAGCTT ACGCGTACCC AGCTTTCTTGTACAAAGTGG TCCCTATAGT GAGTCGTATT ATAAGCTAGA CACA MAP3a ATCCAGAATTAATAAACCCT AGTAAGTGAA ethylene-responsive SEQ ID N° 285 AGTGAAAGAAACTACTCATC CAAATATCTA transcription factor TAGAAAAGTA AATGAATCCCGCTAATGCAA CCTTCTCTTT CTCTGAGCTT GATTTCCTTC AATCAATAGA AAACCATCTTCTGAATTATG ATTCCGATTT TTCTGAAATT TTTTCGCCGA TGAGTTCAAG TAACGCATTGCCTAATAGTC CTAGCTCAAG TTTTGGCAGC TTCCCTTCAG CAGAAAATAG CTTGGATACCTCTCTTTGGG ATGAAAACTT TGAGGAAACA ATACAAAATC TCGAAGAAAA GTCCGAGTCCGAGGAGGAAA CAAAGGGGCA TGTCGTGGCG CGTGAGAAAA ACGCGACACA AGATTGGAGACGGTACATAG GAGTTAAACG GCGGCCGTGG GGGACGTTTT CGGCGGAGAT AAGGGACCCGGAGAGAAGAG GCGCGAGATT ATGGCTAGGA ACTTACGAGA CCCCAGAGGA CGCAGCATTGGCTTACGATC AAGCCGCTTT CAAAATCCGC GGCTCGAGAG CTCGGCTCAA TTTTCCTCACTTAATTGGAT CAAACATTCC TAAGCCGGCT AGAGTTACAG CGAGACGTAG GCGTACGCGCTCACCCCAGC CATCGTCTTC TTCATGTACC TCATCATCAG AAAATGGGAC AAGAAAAAGGAAAATAGATT TGATAAATTC CATAGCCAAA GCAAAATTTA TTCGTCATAG CTGGAACCTACAAATGTTGC TATAACTGTA TTTAATTTGG AAGGAATTAA TTAAGGTTAT TCTATGTCTTTGTATTAGAA TTTAGAATAA TTCCCTAAAG CTCCTGAAGA ACGAAACTTG TAAACATCTCTCTGTCTCCG TATCATGTTC TAATTTAACA TGAAATTACA TGAGCGCAAA AAAAAAAAAA AAAAMAP3b TTGGGGGAGG TTCGCGGCGA AGATAAGGGA AP2-domain DNA- SEQ ID N° 286CCCGGAGAGA AGAGGCGCGA GATTATGGCT binding protein AGGAACTTAC GAGACCCCAGAGGACGCAGC ATTGGCTTAC GATCAAGCCG CTTTCAAAAT CCGCGGCTCG AGAGCTCGGCTCAATTTTCC TCACTTA MAP3c TTGGGGGAGG TTCGCGGCGG AGATGGAAGC putativeprotein SEQ ID N° 287 ACTTATGGAG GCCAAAGGGG TGAGCAAGTA At5g28830 [A.TATCGAAGTG CCAGGTGCTC TCCTTCCCCA thaliana] GGAAGAGTAT CCTGAAATAGTTGCAGAACA GCTTTACAGG TTTCTGCAAG AGAAGTTTGA GCTTCAGGCT TA MAP4bTTGGGGGAGG TTCGCGGCGG AGATGCACTC calmodulin-related SEQ ID N° 288CGTTATGAAG GGCATTGGAG AGAAGTGTTC protein GCTTA MAP5GGCCGTGGGGGAGGTTTGCGGCTGAAATAAGGGAC AP2-domain DNA- SEQ ID N° 289CCGGAGAGAAGAGGCGCGAGATTATGGCTAGGAA binding proteinCTTACGAGACCCCAGAGGACGCAGCATTTGGCTTAC GATCAAGCCGCTTTCAAAAGCCGCGGCTCGAGAGCTCGGCTCAATTTTCCTCAC MC101 TAAAGGCGCC GACTATGCTG CATCATTCTG putativeprotein SEQ ID N° 290 GGCTGAGGTA TTTGATGGGG TGAGGCAGAG At3g06150 [A.AGGGTTGACA CCACCAGAAG TAATATATAG thaliana] GACCACAGTCACCACAGGCGGATACGCTAG AAGATTGGCA TTCAATCCAA ATAAAATGGA GGCCTTCAAT GGGGTAGTCTTGGATAAGTT GAGGGCATAT GGTTTAGTTG ATCGCGTCAT TGATGATTTC GACATGACTTATCCTTGGCA CTATGATAACCGATGCAATG ACGGGGTGCA TTATGGCCGT GCTCCTGCCA AGMC102 TAAAGGTGGA GAATATTTTG GTGATGGGAC carbonic anhydrase SEQ ID N° 291ACAGCTGCTG TGGAGGTATA AAAGGACTCA TGTCTATCCC TGATGATGGC TCCATAGACAGTCATTTCAT CGAAGAATGG GTCAAAATCT GTTTGATATC AAAGGCAAAG GTAAAGAGAGAACATGGCGA CAAGGATTTC ACTGAACAAT GTACAATATT GGAGAAGGAGGCAGTAAATGAATCACTAGC CAACTTACTG ACATATCCAT TTGTGAGGGA AGCTGTG MC104 TAACCTTGGAAAGACATGGG AGAAGCTGCA P40-like 40S SEQ ID N° 292 AATGGCTGCG AGGGTTATTGTTGCTATTGA ribosomal protein GAATCCAAAG GACATAATTG TGCAATCAGC CAGGCCCTATGGCCAGAGAG CTGTCTTGAA GTTTGCTCAA TACACTGGCG CAAGTGCCAT TGCTGGCCGTCACACTCCCG GTACTTTTAC CAACCAGCTT CAGACTTCAT ACAGTGAGCC CCGACTCCTCATTCTCACTG ACCCAAGAAC TGATCACCAG CCTATCAAGG AAGCTGCACT TGGGAACATCCCTACTATGG CTTTCTGTGA CACTGATTCA CCGATGCGCT ATGTTGACAT TGGTATCCCTGCCAATAACA AAGGGAAGCA CAGTATCGGT GTTCTTTTCT GGCTCTTAGG AAGGATGGTACTGCAGATGC GCGGTAGCAT TCCTCAGGGA CACAA MC105 TAACAGACGT TGATGATATGATGTTATGGG alanine acetyl SEQ ID N° 293 CAGGCGACGA TCGAGTAACT AGGACCATCCtransferase-like GATGGAAAAC TTTGACCTCG AAAGAAGAGG protein CATTGGCCTTCATCAAGGAA GTGTGTATAC CTCACCCCTG GCGTCGATCA ATATGCATCG ATGACCGATCGATCGGGTTT GTATCAGTAT TTCCTGGATC AGGTTATGAT AGAAGCCAAG GTGTCATAGGATATGATATT GCAGTTGAAT ATTGGGGGCA GGGGATTGCT ACAAATGCTA TCAAAATGACAATCCCTCAA GTGTACAATA ACTTTCGTGA AATAGTAAGG CTTCAGGCAT TAGCTAATGTTAAGAATAAG GCATCCCAAA GGGTGTT MC106 AATTCCCCCATGTGCATGCCTGAGTGCACAAACAGputative late SEQ ID N° 294 GAAGGCGAATTGCAATCACCCCGGAGCAGCATGCTembryogenesis TGGATC protein MC107a TAACCCAATTTTGTTGCCAAAGAAAACTGGAGGTGhistone H2A-like SEQ ID N° 295 AAAAGGCTGGCAAAGAACATAAATCTCCTTCCAAAprotein GCAACCAAATCTCCTAAGAAGGCTTAGATTTAGTGGCTGTTATAAGCCTCTTGCTTTTCTATCTTTATTTGG ATC MC107bTAACACGGGAATGATACCAGAGATACAGGCTACAG proline transport SEQ ID N° 296TCAGACCACCTGTAATTGAGAACATGTTGAAAGCT proteinCTGTTCTTTCAGTTCACAGTGGGAGTTGTGCCCTTGCATGCTGTTACTTATATAGGTTATTGGGCTTATGGA TC MC108 TAACAACCCC ATTTGGAATAGCACTTGGAA putative metal SEQ ID N° 297 TTGGTTTATC AAAAGTGTAT AGTGAAAATAtransport protein GTCCAACAGC ACTA MC109CGTTCGTGGGACCTACAAGGGGCGCGAGGGCAAAG putative 60S SEQ ID N° 298TCGTTCAAGTGTACCGTCTGAAATGGGTAATTCACA ribosomal proteinTTGAACGCAGTAACACGTGAGAAGGTTACTC MC113 AGTAAAGGTG CAGAATATTT TGGTGATGGGputative carbonic SEQ ID N° 299 ACACAGCTGC TGTGGAGGTA TAAAA anhydraseMC114c GATCCAGCAG AGTCGGAGGT TGCCGGATTT putative beta- SEQ ID N° 300CCTTCAGAGT GTAAACTTGA AGTACGTTA ketoacyl-CoA synthase MC115 TAAGCACCCTAGTATTTCTG CATACATGGG putative SEQ ID N° 301 ATCAAGACTC GCTGGGAAAGTTTTGGCAAC Dihydroorotase CTTTGTGCGC GGAAATCTTG TATACAAGGA GGGAAATCATGCTTCTCTTG CATGTGCTCT CCCAATTCTG CATAGATAGT TAGTGCATGA GCCTATCAGTAACTCCACCA ACTTACCATA TATCATCCAA ATTATTTCTT CTGTGCAATC TTCATGTTCTTTGTTGTGTC CCTTTGACAT TCTTGGAGAT GACCATATGG CATGATATAC AGATGGAATTGGTGACTTCC ATCATTT MC116 TAAGCAACCC GAAACCCGAT CCGAACCATT putativeprotein SEQ ID N° 302 CAACTCGGAC TAAGTCGGTT CGGACCGAGG At1g71780 [A.TTCCGGAGGT CAAGGTCCAC CTGTATCGGC thaliana] AAGGCAAGGG TCCTATCGACGAATTCACGA TGCCCTTAGG TGGATGGGAC CAGGATCAGC TGGAGGTTCG TGAAATTCTCGACAAATACG GGTTCAAATC GGTCTATGCA TTCAAACCGG ATACGGGTCG GGGCGTTCCCATCAGATTCA ACCCCCGTAA CGGCCGATCT A MC118 TAAGGTATTT GTGAAGTCTTACTATTTTCC N-acetyl-gamma- SEQ ID N° 303 ACAAGGAGAG ACTGCTTCAAGATTTTTTGT glutamyl-phosphate GGAAGAGTTT TGTTTGCTGA GTTTGTAATT reductaselike TCTGTAGAAG TATTCCCGTG TATCCTGGCG protein TAGTTTTCAG ACGTACCCTATATTTGATTG CTAATTTTAT GCCTCAGAAG GAGATTATGT GCCATAGATA AAGTTGAACAGGGGGGTGGA TC MC121a AGTCCTATGTGATTGCAAGAGACCGATTTCTTGTTC putativearginine SEQ ID N° 304 AAAATGGAAAAATGTTTCCTGGTGGCGGAAGAATAmethyltransferase CACATGGCACCATTTAGTGACGAATATTTGTATATGGAAATAGCAACTAAGGCGACCTTTTGGCAGCAACA AAACTACTTTGGGGTTGACTTGACACCCTTGCACGGATC MC121b GCGACTTCCGCTTTCGGTACAGTGCAATCTTCTACC 6,7-dimethyl-8- SEQ IDN° 305 TCGTGCAACAACTGTAAATCCCACACAACTGCACT ribityllumazineCTCCTCTTTACTCTTTGTCTCTGCCTTTCCACAGACA synthaseAAGCATAACCTCTTCACCTGCACTATCATTCACCCA ATCTCAAGGTTTAGGGTCTGCAATTGAGAGACATTGCGACCGGTCGGATC MC123 TAAGCAAAGA GAGGCAGCTT GGTTTGCTGG putative proteinSEQ ID N° 306 TTCTGTGAGA TCAAGACTAC AGTATTTGGG At2g46580 [A. GCCCACTCCAGGACTTCCTT CTCTAGATGA thaliana] GCAACCATTG CACGACTCGT TGGATC MC124CGGGCCCAATTTGCCCTATAGTGAGTCGTATTAAA putative protein SEQ ID N° 307AGCAGGCAAGCCTGTTGGTGGGTTCAAGATAGGTA At1g50570 [A.GACAATCTGGGGAATGGACGGGTTAAAATTTTCAT thaliana]CCGTACTTCCATCAGAGAGTTATCTTACATGCAGG TTTTTCTCTGCAAGGAAACATGGGTTGGTGGATGCTGTTGTGAGATGTAAAAGCTCCGAGCGGACAGCTGT TGTCGCCCTTCCTGGTGGAATTGGTACCCTTGACGAGATTTTTGAGATTATGGCTTTGAT MC125a TAATCTCAAT GCATCTTTGT TTGTTTGAAT acylcarrier protein SEQ ID N° 308 TTGTTCATCA AAATCAAAGG TACACTTGCTCCTTGTCATT TGACTAGTTC AAGGTTGTAG AATTTTGATC CTCTTGAGAG AGGCAATAATCAGACTCTTT GGAAGACCAG TTGCTCAGGC TTTGCCATTG AGGATTATAT CATCCTTTTGTTGCTTTTCT GGAAGACATG ACTCAGTATT TATTCTGTTG CCGTCYLTCC TCTTATAATATTCGAATGCC ACAAATTCAA GCTTGGTTTG ATTGTTGCAC TGATTTGAAA AATCTGTCTAGTCTGGCTCA TGAACTTGTG AAGCTGATGC TGGATC MC125b TAATACAGAA GCCTTACTCTATTGTGTACT putative protein SEQ ID N° 309 TCCATTCTGC TGCAACCTTACAGATTCAAC At1g69340 [A. CAGATCTAGG ATTGATGAAG AGAATACAAC thaliana]AAATACTCGG TCGCAAGCAC CAGCGCAACC TTCATGCGAT ATATGTTCTT CACCCTACTTTTGGACTGAA GAGTGCAATA GTTGCACTAC AGCTCTTTGT GGATTATGTG GTATGGAAAAAAGTAGTGTA TGTAGATCGT CTTCTGCAAC TATTCCGCTA TGTTCCTCGT GAACAGCTAACCATCCCAGA TTTTGTATTC CAGCATGATT TGGAAGTAAA TGGAGGGAAG GGCCTAATTG TGGATCMC126 TAATGGATGC TGCAACGCAA GGTGCCCTAC putative protein SEQ ID N° 310AAGCAGGGAA GCCTGTTGGT GGGTTCAAGA At1g50570 [A. TAGGTAGAGA AGCTGGGGAATGGACGGCTT thaliana] CAAATTTTCA TCCGTACTTG CCATCAGAGA GTTATCTTACATGCAGGTTT TTCTCTGCAA GGAAACATGG GTTGGTGGAT GCTGTTGTGA GATGTAAAAGCTCCGAGCGG ACAGCTGTTG TCGCCCTTCC TGGTGGAATT GGTACCCTTG ACGAGATTTTTGAGATTATG GCTTTGATTC AACTCGAACG AATTGGATC MC129 TAAGCAACCC GAAACCCGATCCGAACCATT putative protein SEQ ID N° 311 CAACTCGGAC TAAGTCGGTTCGGACCGAGG At1g71780 [A. TTCCGGAGGT CAAGGTGATG AGTCCTGAGT thaliana]AATGACAACA ATATAGCATC ATTGGTAGG MC130aGATCCAAGAAGCTCTTTTGCCTAGCCTTATGAGTAA G protein beta SEQ ID N° 312TTTTATGTTTCCTTCTGTGTTTTTCTTACAGATCTTT subunit-like proteinTCCGCAGTAGAAGTTTTGTTTGGATTA MC130b TGAGTATGTG GTGTGTTTGT CCAAAAGGTAputative protein SEQ ID N° 313 GATTTATTGA AAAGTATCAA GCAGCTCAAGAT3g45540 [A. TGTAGATGTG GTCATCTAAC AAATGGTGGA TC thaliana] MC203TAAAGGTGCA GAATATTTTG GTGATGGAAC carbonic anhydrase SEQ ID N° 314ACAGGTGCTG TGCAGGTATA AAAGGACTCA TGTCTATCCC TGATGATGGC TCCATAGACAGTCATTTCAT CGAAGAATGG GTCAAAATCT GTTTGATATC AAAGGCAAAG GTAAAGAGAGAACATGGCGA CAAGGATTTC GG MC204 ATGTATGGTA GATCAGGGCT TGATCGATTT putativeprotein SEQ ID N° 315 AAGAAAGCTC AGTCATTGGA GCCATTTCAG AT5g47790 [A.GTGTCTGCGA ATTCAGCTGC TAAACCAGCA thaliana] TTGCAGCCTA CTACAAAGGCGGTTACACAT CCTTTTCCAG CATATGCACA ATCCACAACA TCTCATCAAC AAACTCAATACGTAAATCCA CAACCTGCTT TGCAGAAATC CGTGGCGGCA GATGCAACCG CTTCTACAGTGCCAACTCAT CATGTCACTC ATGGAGGGGG ACAATCAACT TGGCAGCCTC CTGATTGGGCTATTGAGCCA CGTCCAGGAG TTTATTATCT TGAGGTGATC AAGGATGGTG AGGTACTCGATCGAATTAAT TTGGATAAGC GAAGGCATAT CTTTGGACGG CAGTTTCATA CTTGTGATTTTGTCCTTGAT CATCAGTCAG TCTCACGCCA GCATGCTGCT GTGATTCCTC ACAAAAATGGAAGCATTTAT GTGATTGATT TAGGATCTGC ACATGGAACA TTTGTAGCAA ATGAGAGGCTAACAAAGGAT TCCCCTGTCG AACTTGAGCC CGGACAATCT TTGAAGTTGG CTGTATCAACAAGGCCTTAC ATCTTGAGAA GGAACAATGA TGCTCTCTTC CCTCCTCCAC GGCAACTGGCAGAAATAGAT TTCCCGCCAC CTCCAGATCC TTCAGATGAG GAAGCTGTTT TGGCTTATAACACCTTTTTA AACCGCTATG GGCTTATAAG GCCTGATTCA TTGTCAAAAT CAACAGTATCAACTAGTGGG GAGGATGTCA ACTATTCATC TGACAGGCGC GCGAAAAGAA TTAGGAGAACAAGTGTGTCA TTTAAAGATC AGGTTGGAGG AGAGCTAGTT GAAGTTGTTG GTATTTCGGATGGAGCAGAT GTGGAGACAG AACCTGGTCC ATTGGGTGTG AAAGAAGGAA GTCTTGTCGGAAAATATGAG TCCCTAATAG AACCTACAGT GATACCGAAA GGGAAAGAAC AGTCCTCTGTAAAGGATGCC ACCGTTACCC GAACAGGTGT ATCGGACATA CTTCAACAGG TATTGTCCAAGGTGAAAAAT CCGCCGAAGG GTGGAATTTA CGACGATCTT TATGGAGAAT CAGCTCCTGCTAAAGGGGGA TTTTGGGCAT ATTCTGATTC CAGTCAAACA GCTTCTACTA ACGACGCTAAAGGAGACTCC CCTTGTTCTT TACGCAGAAT CTTTGGACAT ATCTCAAACA ATGTAGACGACGATACCGAT GATTTGTTTG GATAG MC205 TAAAGCAGAT TTGCTCAACA TTACTCAACTputative protein SEQ ID N° 316 TTCTGAGTAT AGAAAAGAAG CA At3g11030 [A.thaliana] MC207a GAGTCCTATGTGATTGCAAGAGACCGATTTCTTGTT putative arginineSEQ ID N° 317 CAAAATGGAAAAATGTTTCCTGGTGTCGGAAGAAT methyltransferaseACACATGGCACCATTTAGTGACGAATATTTGTATAT GGAAATAGCAAATAAGGCGACCTTTTGGCAGCAACAAAACTACTTTGGGGTTGACTTGACACCTTTGCACG GATG MC207bACTCTCTCTTCCACTGCTCAGACAACAATCGAAATT heat shock protein SEQ ID N° 318GATTCTCTGTATGAGGGGGTTGACTTTTATCCTACC 70ATTACTCGTGCTAGATTCGAGGAGTTGAACATGGA TC MC209TAACAAAACAAGCAGTGGCAAGGAGTTCCCAGTGA EEF53 SEQ ID N° 319CAGCTTTTGTATTCGCAAGTCCTAAAGTTGGGGATC MC210bTAACGAAGAAAACAACAACAACAATAACAACAAC putative protein SEQ ID N° 320AACAACAAGCCCAGTGTAATCCCACACGTAGGGAT AT3g24200 [A. C thaliana] MC212TAAGGAGGCT GTAGAATTGA TCAATGGGAG quinolinate SEQ ID N° 321 GTTTGATACGGAGGCTTCAG GAAATGTTAC phosphoribosyl CCTTGAAACA GTACACAAGA TTGGACAAACtransferase TGGTGTTACC TACATTTCTA GTGGTGCCCT GACGCATTCC GTGAAAGCACTTGACATTTC CCTGAAGATC GATACGGAGC TCGCCCTTGA AGATGGAAGG CGTACAAAACGAGCATGAGC GCCATTACTT CTGCTATAGG GTTGGAGTAA AAGCAGCTGA ATAGCTGAAGGGTGCAAATA AGAATCATTT TACTAGTTGT CAAACAAAAG ATCTTGGGAC GGTGAGCTCCGTTTGTGGGA TC MC214 TAAGGTAAGG CACAATAATG TCGTTCCTAT putative pyruvateSEQ ID N° 322 GATGGCTTTG GGAGTCCAAC AACTCAAGAA dehydrogenase AGATTGGCCTAAAGTTGATT ATGAGGATTT kinase GAGAGAAATA CACCAAT MC215 TAAGCCCGAGAGGTTTCTTG GCTCGAAAAT cytochrome P450 SEQ ID N° 323 AGATGTGAAAGGGCAGCATT ATGAGCT hydroxylase MC216 TAACGACTGC AGAATCATCT ATATACGAAGputative protein SEQ ID N° 324 TGCTTGAATC CCATGGATTG CCAATGGGTTAt3g07460 [A. TACTTCCAAA AGGTGTGAAG AATTTCACAT thaliana] TAGACAATTCGGGGAAATTT GTAGTCCATT TGGATCAAGC TTGCAATGCT AAATTCGAGA ATGAGTTTCACTATGATAGG AATGTATCGG GTACAATAAG TTACGGACAG ATCCATGCAC TTT MC219GGAATCGAACTAATCGCATCGGAAAACTTCACATC glycine SEQ ID N° 325ATTCGCCGTAATTGAAGCTCTCGGCAGTGCCTTA hydroxymethyltrans ferase MC220GATCCCTATTTTACAAGAGTGCATTGATGCCATCAC putative protein SEQ ID N° 326TGAACACCAAAGGCTTCTGTCCTTA At1g07970 [A. thaliana] MC222 TAATAGGTATAGCATGCCAC AAATCTGGAG ambiguous hit SEQ ID N° 327 TTGAGGTGGT TATTCTTATACCCCCAAATG CCCCCAGCAT AGCAGCTTAT GGTTCCATTG TTGTTGT MC223TAATGAGACAATGAGATTATACCCTCCGATACCAC cytochrome P450 SEQ ID N° 328TTTTATTGCCTCATTATTCAACTAAAGATTGTATT G MC225TATTGGTACGTCGTAAAATGTGACCGGAAAACCAA polygalacturonase SEQ ID N° 329CCGGATTA inhibitor MC302 CCCCTATATT TTTCCCCTAT ATCTTTTTCT CCTCCCpoly(A)-binding SEQ ID N° 330 protein MC304 TAACGACTGC AGAATCATCTATATACGAAG putative protein SEQ ID N° 331 TGCTTGAATC CCATGGATTGCCAATGGGTT AT3g07470 [A. TACTTCCAAA AGGTGTGAAG AATTTCACAT thaliana]TAGACAATTC GGGGAAATTT GTAGTCCATT TGGATCAAGC TTGCAATGCT AAATTCGAGAATGAGTTTCA CTATGATAGG AATGTATCGG GTACAATAAG TTACGGACAG ATCCATGCACTTTCAGGAAT TGAGGCTCAA GATTTGTTTC TATGGTTTCC AGTGAAGGAT ATTCGGGTTGATATACCCAG TTCTGGTTTG ATTTACTTCA ACGTTGGCGT TGTATCTAAG CAATTCTCTTTGTCTTCATT TGAGACTCCT AGGGATTGTA CTG MC305b TAACATTGTT TACAGAAGAAAAGCAGGGGG Plastid-specific 30S SEQ ID N° 332 TTATGGACTT ATTATTCCCAAGGAAGATGG ribosomal like TAAGACAAAG TTAGAGCCTG TGGAGGTTGA proteinACTAGAGAAA GAAACGTCGA TGGCAGAATA GAAGGAATTG ATGAAAAGTG ATTAGTTAGTGACCGAGTAC ATTTACTTTG CGTTACGATC ACTTTTGTAG AGAAGGTTTT CTGCTTGAGGATGTTTTTGC ACCCATCATC TGCGACAGAC TGACGGAGCA CTACGCA MC306bTAACCATGCTCTTACAGGATTCTTTTGAGGATGACA kinesin like protein SEQ ID N° 333AGGCCAAAATTCTCATGATACTGTGTGCGAGCCCG GATC MC307 TAAGGCTGCT GGTGAAAGAAGTGGCGGATC putative protein SEQ ID N° 334 TCTCGATGGT GTAGCATTTCTCCTAAGTTC At2g44090 [A. AGATTTCCTT GGTGATCCAG CTGCAACTTA thaliana]TGCGGTCGCC GACAGCATCG CTAAGTCGGA TGACGAGGCT GTCGCTCCTG AGCTCAGGTCTTTCCTTCGG GAGCATTGGT CGGAAGCTGC TTTCTCAGAC GGGCTTAGGC AAGGACAAGAACACTACTTG AATATCGTGC GTATTTTGAA ATGGGGGGAA MC308TTGGCAGTGAGATTTTTGCGAATGATTGAGGCTGCT putative Pto kinase SEQ ID N° 335GTCATCTTGTGTGCGCCACTCATGCTTCAAAGAGAC interactorCAGCAATGGGACAGGTAACACTTGTTCCATTTTATT GAATGAAAACCTATGCCAGAAACGCCCTTAMC309a TAATGGTCTA GCATCGGAGG ATGCTCTGGG polyprotein SEQ ID N° 336ATTTCTTGAG GAGTGTTACT GCATTCTCCG TACTATGGGT ATCTCAGGAT CGAGCGGGTTTTCTTTCACT ACTTTCCAAC TTCGAGGAGT CGCGTATGAT TAGTGGCACA CCTATGAGTTAGACAGTCCA GATGAGGCTG CTTCACTAAC TTGGGCTCAG TTTTCGGAGC ACTAC MC309bGATCCGAGCA TTGTGGAGGC ACTATTTCCA ADP-ribosylation SEQ ID N° 337GAACACTCAG GGTCTCATTT TTGTGGTTGA factor-like protein TAGCAATGACAGAGACCGTG TCGTGGAGGC AAGAGATGAA TTGCACAGGA TGTTGAACGA GGATGAGCTTCGGCATGCTG TGCTGCTTGT TTTTGCTAAC AAACAAGATC TTTTCCGCAG TAGAAGTTTTGTTTGGATTA MC310a GATCCGCCGCACAGACCAAAACACCGCCCAGCGTA zinc finger likeSEQ ID N° 338 GGCTTTTCATCTTCGTCAATATTAGCAAATTAGAAC proteinCCCCACCCATTCTCTTCTTTTTCAACAACAGCCAAC CCTCAGCTGCCGACACACACGCACAGTCGCCGATGGACAGAGAATCAGCGAATGCCATAGCCATTTGCTGC CTCTGCTTCTTCCCATTA MC310bAATGAAAGAATGTTGGAGTCCTATGTGATTGCAA putative arginine SEQ ID N° 339GACACCGATTTCTTGTTCAAAATGGAAAAATGTTTC methyltransferaseCTGGTGTCGGAAGAATACACATGGCACCATTTAGT GACGAATACTTGTATATGGAAATAGCAAATAAGGCGACCTTTTGGCAGCAACAAAACTACTTTGGGGTTG ACTTGACACCTTTGCACGGATC MC311aGATCCGACCA AGGCGTCTTA GCATTGAAGG eukaryotic initiation SEQ ID N° 340CCTTGAAGCT TTCCGATTCT TTCATGGAAC factor 3H1 like TCTACAAGAG TAACAACTTTACTGGAGAGA protein AGTTGAGGGA AAAGACTCTT TCATGGGTCG ACATCTTTGAAGAGATACCG ATTA MC401 CAGAATCATC TATATACGAA GTGCTTGAAT putative proteinSEQ ID N° 341 CCCATGGATT GCCAATGGGT TTACTTCCAA At5g19850 [A. AAGGTGTGAAGAATTTCACA TTAGACAATT thaliana] CGGGGAAATT TGTAGTCCAT TTGGATCAAGCTTGCAATGG TAAATTGGAG AATGAGTTTC ACTATGATAG G MC402GATCCTACAATCAACCTGAGAACATGCATAATTTA putative beta-1,3- SEQ ID N° 342TGTTTTCTTGTAGTGTTTTTCTGATCTGATGAAGGTTT glucanaseAGCTACACACCAAGTTTTCTTTTCATTTGCTAACAC CAATGTTCCCACTGAAATGTGGGACAAAAGTAGGAAGCAAAGGGTGAGAGCTGCTTTA MC404 TAACTTCAAT GCGACCAGTG GTGCTCGGATnucellin-like SEQ ID N° 343 AATACCTCGT TTGGCTCTAG GGTGTGGATA proteinTGATCAGTTA CCTGGTCAAT CTCATCATCC TTTAGATGGA GTGCTTGGCC TTGGGAAAGGAAAAGCCAGC ATTGTGTCTC AGCTTCACAG CAAGGGTTTG GTGCGGAATG TGGTAGGCCATTGCTTGAGT GGCACAGAAG TAGGTTTTCT CTTCTTT MC405 TAACGAGTAT GGCGAAGCCTATGAATCCCA NADH SEQ ID N° 344 TGCTGAGTTT CGTTAGTTCA AGGCCAGGATdehydrogenase GGGTCATGCT CTCAAGTTAC TCGTGTATGA subunit 1-like TTTTTTTTAGTCTTGGCAAA TTTTTATGCG protein AGTCTCACCA AAAGATGCAT GTGTGTGTA MC406aGATCCTAGCATTTGAGAAGTTCCTTGAAGAAAACC trehalose-6- SEQ ID N° 345CATACTGGCGTGATAAAGTGGTTTTGCTGCAAATTG phosphate synthaseCTGTGCCAACAAGAACAGATGTTCCTGAATACCAA AAACTTACTAGTCAGGTTCATGAGATTGTTGGACGCATCAATGGCCGGTTGGAACTTTGACTGCAGTGCC TATTCATCATCTGGATCGATCTCTTGACTTTCATGCATTATGTGCACTATATGCTGTAACTGATGTAGCGCT GGTTTACCTCCTTA MC406bTAAGGGGTTTTGAGTTTTGTTTACTACTACCACTGC Nicotiana tabacum SEQ ID N° 346TCTCAGAAAAAATGGATTTGATAGTCTAGTTTTTTA RENT3 repetitiveCACAAACTCTTTTCAAACTATGTCAAGCACTCTCAC sequenceATATACTCTTTAGAATACTAGGTTCTGCCCCTCTTGTGTGAGCTTTGCCTTGGGACCCTTGAGCTCTCTCTGA ACTTGGACACATAAGAGCTGGTCCTTCCATACTACACTTACTCTTGGTTATGCAATCTGGGTGTGAGCACT ACCTAGGATC MC406c TAAGGGAGCTGTTCCAGTTC CAGAGTCAGT 60S ribosomal SEQ ID N° 347 GCTGAAGAAG CAAAAGAGGAGTGAGGAATG protein L7 GGCCCTTGCA AAGAAACAAG AGCTTGAAGG TGCAAAGAAGAAGAGTTCCG AGAACCGGAA ATTGATCTAC AACAGAGCTA AGCAGTATGC TAAGGAATATGAGCAGCAGG ATAAGGAGTT GATTTGCTTG AAGCGCGAGG GTAGATTGAA GGGTGGTTTCTATGTTGACC CTGAGGCAAA GTTGTTGTTC ATCATTAGGA TC MC407 TAAGGCAGAGATGTTCTTTG ATAGAGGAGA putative SEQ ID N° 348 ATTGCTTGGA GGCCTTGTGAAAGGAGAAAG pathogenesis related CAATGGTGAA TTGGCATTGG CTGCTTCAAA proteinATGTCCTTTC ATGAAATAAG AGCAAAACCA GCAACTGCTG CTTATTTTCA AGACAAGATCTCAAGAAAG MC408 TAAGCAGGGG AGGAAGTACT GCAAAATTGG cytosolic pyruvate SEQID N° 349 TGGCCAAGTA CAGACCTGGA ATGCCTATAT kinase TGTCGGTGGT TGTCCCCGAGATCAAAACTG ATTCTTTTGA TTGGACTTGC AGTGACGAGT CTCCAGCAAG GCATAGCCTTATATTCAGGG GAT MC409 TGTATAACCTTTTTGATGTCTCAATTCTTATGCTCTT putativeprotein SEQ ID N° 350 ATGAATAATACATAACAATTGCCACGAAATTTTCT At1g80220 [A.GAAAGAATAGGTGGCTTA thaliana] MC410 TAATGTTTGG CTACTCTTCT GTACAGCTTCputative protein SEQ ID N° 351 CAACATTGGA CAAGGATAAC CTCCGCGGTGAt4g28910 [A. TGGCtTCTCA TCTTCAACAG CTTCACCCTT thaliana] CCCATGGAAGAGGTCCTCTG GGTTCAGATA TGCAGAAAGA TGGACCAAAT ATTTCTCAAG CTACTACGTCATCTATTCCG CACAAGTCAT CTGATTCTGT ACAATATGAT GGGAGGGCAA TGGAGCATGTGAAAGGCAAT GGGAGACAGC ATAAGGCAGA AGAAACTTCC AATTCTCGAG GGGAGGAAAATGTGAAAGGA AGCAACATAA GCTTCAGGGC AAAAGACCCT CCTGACCAGC CCAGAGCAGAAGCAGTTCCT TCTAATTTTC AACTATTAGG CCAGGTCTTG CTGCAGAT MC412 TAATCGCATTGAAGCACGGA GTGAGCAGTT RNA polymerase I, SEQ ID N° 352 TGACATGTACATGCTGTTGG ATGTGAACAC II and III 16.5 kDa TGAGATATAT CCTATGCGCGTCAAAGAGAA subunit ATTTATGATG GTTTTAGCAT CTACTTTGAA CTTGGATGGGACACCAGATA CTGGTTATTT CATTCAGGGT AACAAGAAAT CACTTGCTGA CAAGTTCGAA TATGTCMC413 TACCTGTGGTTGGATCGGTATAGTCGCCACGGTCAC putative esterase SEQ ID N°353 TCGCTTGACCTACTGTCACTGGGCTACCTAAAGTCAACACCACGTTATTACCCACTACCGGAACACCGGTT ACAGTCACCAATTGACCACCAGCAGTCACTGTAAAGCTACCTGTTGTTGGCAAGTGCAGTGGATTA MC414 TAACGAGTAT GGCGAAGACT ATGAAGCCCAputative calcium SEQ ID N° 354 TGATGAGTTT CGTTAGTTCA AGGCTAGGAT bindingprotein GGGTCATGCT ATCAAG MT101 ATGAGAGTTC GAATCCACCA AACAATGGCGGTP-binding-like SEQ ID N° 355 ACCGTTATGC AGAAAATCAA AGATATCGAA proteinGATGAGATGG CTAAGACCCA AAAGAACAAA GCTACTGCTC ATCATCTCGG TTTGTTAAAGGCAAAACTGG CAAAACTTCG AAGGGAGCTT CTTACACCTA CATCAAAAGG TGGTGGTGGAGCTGGAGAAG GTTTTGATGT TACAAAAAGC GGTGATGCAA GAGTGGGTTT AGTGGGCTTTCCTTCAGTTG GAAAGTCGAC ACTCTTGAAC AAATTGACTG GAACTTTTTC TGAGGTTGCTTCATATGAAT TTACCACCTT AACGTGCATT CCTGGTGTCA TCATGTATCG AGGAGCTAAAATCCAGTTGT TGGATCTCCC AGGAATTATT GAGGGTGCCA AGGATGGAAA AGGTAGAGGAAGGCAGGTTA TCAGTACTGC AAGGACTTGC AATTGTATAC TTATTGTTCT TGATGCAATAAAACCAATTA CTCACAAACG TCCCATCGAG AAAGAGCTTG AGGGATTTGG CATCAGGTTGAACAAGGAAC CACCTAATCT GACATTCAGG AGGAAAGAGA AGGGTGGGAT CAATTTAACATCAACAGTGA CCAATACTCA TTTAGACCTC GACACCGTAA AGGCCATATG CAGCGAATACAGAATACATA ATGCTGATGT TCATCTTAGG TATGATGCAA CTGCTGATGA CCTTATTGATGTCATTGAAG GCAGTAGAGT ATACACACCT TGCATCTATG TTGTGAACAA AATTGATCAAATCCCAATGG AAGAGCTGGA GATTCTGGAT AAACTTCCCC ATTATTGTCC GATCAGTGCTCATTTGGAAT GGAATCTTGA TGGCTTGCTG GAGAAGATTT GGGAATATCT CAGTCTAACCCGTATATACA CTAAGCCGAA GGGAATGAAT CCAGACTATG AGGATCCAGT AATTCTATCATCAAAGAGGA GGACAGTGGA GGACTTCTGC GACAGAATCC ACAAGGATAT GGTTAAACAATTCAAATATG CGCTGGTTTG GGGTTCAAGT GCAAAACACA AACCTCAGAG GGTGGGCAGGGAACATGAAC TAGAAGATGA AGACGTCGTC CAAATCATCA AGAAGGTGTG A MT102TAAAAGGGAG AGAGCAGAAC GTGAGGCTTT ubiquinol-- SEQ ID N° 356 GGGAGCTTTGCCTCTCTATC AGCGGACAAT cytochrome-c TCCATGAAGA AATCAAATCT CCCTTGAAGCreductase-like TTTTTCGATT GAGAATAATT ACTGTGTTGC protein TTGTAGATGAGCTTTGCCTC TGTATCAGTC GTACAATTCC ATGAAGAAAT CGAATCTCCC TATAAGTTTT TCMT103 TAGCAACTTTGACAGGTGTCAATGTCGGTGACAAT pathogenesis related SEQ ID N°357 GCAACAGCACAACGAGGTGATTATGCCTTCAGTTT like-proteinCACAGTAAATTGATCGATATTGGGCTATCGATCAA TATGCCTTCAGTTTCAGAGTAAATTGATCGATATTGGGCTATCTTTGTTTCTGAAGCTGCATTGTTGAATCTTTTCATCGGATATCCTTCTTGTTGTTCATTCTGTAGCCTAGCTAATTGTGGACTTTCTATTATCGTGTCTTTTT CGTAATATTGCAAGATC MT104aACAACAATCGAAATTGATTCTCTGTATGAGGGCCTT heat shock protein SEQ ID N° 358GACTTTTATCCTACCATTACTCGTGCTAGATTCGAG 70 GAGTTGAACATGGATC MT106aGATCTAGTGG CCGGTGAATC ACTGATCAAA ribosomal protein SEQ ID N° 359GAGCAGATTT TAGAGAGATT CTTCATCGAT CTAGTGGCCG GTGAATCACT GATCAAAGAGCGAGCAGCCG GCAGGTTTAG CCAGAACTCG TCGATCAC MT106bGATGAGTCCTGACTAAACTAATCGATTTGGGTGGC putative ubiquinone SEQ ID N° 360AATGATAGAAGGTAGTCGTCTTCGGTTGAAAGGGT biosynthesis proteinGGCAGCAGGCTGCTGTTGCAGTTGGTTCTGCATTTG GGGCGTTGCTAGATCA MT108 TAACCACAGATTTCTCAAGC TGAATCATCA water channel SEQ ID N° 361 TGTAGCAAAG ATCAAAAprotein MT109 TAACGTGCTC GGAGAACCTG T ATP synthase beta SEQ ID N° 362subunit MT110 TAAGGAGTTG TCACTGGAGC AGGAATCGTT putative protein SEQ IDN° 363 CATCGTAAAG AGTGACCCCA AAAGCTCAGG At1g79140 [A. TACCAAGAGAAAAAAAGGGA GTGCCTCATT thaliana] AGAGCATATT AGTACGGGGT CTGACCTTGATTTCACTGCT CAAATTGATG AAAATGATGT TAGAAAGAAA CTCTCTGAGC ATTACTTGCTGCTTCATGAC ATAGCTGAAA ATGAAAGAGT AAGAGGGGAA TTGGCTCGGA CAACATTGTCTCTGAAGCTG CACGAACAAT ATAAAAAGCA GAAGAAAAGA AGAACATAGT AGGCATCTG MT111TAAGAGCTGT GGAAAAGGTC TGTTGGAATC putative annexin SEQ ID N° 364TATTCTGAAG GTGGTTATCT GGTGCATTGA TTCACCAGAG AAACATTTTG CTGAGGTTGTCAGAGCCTCG ATTGTCGGGA TAGGAACTGA TGAGGATTCT CTAACAAGAG CCATTGTAGCTCGAGCTGAA GTTGATATGA TGAAAGTAAG GGGAGAGTAT TTCATCGCGA ACAAGACCAGTCTTGATAAT GCAGTTATTG GTGATACATC AGGTGATTAC AGGAAGTTCC TGATGACACT MT112TAAGGGCTTC ACAAATGTGA ATCTCAAAAC glucose-1- SEQ ID N° 365 TACGTGTATCCTGGCATTGC AGAAAAAAGC phosphate AGCTATGCTA GCAGGTTTTT TAGCGCCTCAcytidylyltransferase AGCATGAGCA ATATGAATTG TTCCAGTTCT like proteinATGGCATGTC ATGTTATTAT ATCTTCACGC CGATGACAAA ATAATTGAAT GCAGGAAGAAGCTCCTGGTG CTGCCAGAGT ACAAGTTTAC GACTATTTCA A MT113aGATCTACAGTGTTTTTCAGGCTTCAAATTCATCAAC putative DNA SEQ ID N° 366ATCTCACAAAGGAGCTGTTGCTGTTAGGCAGCCTT replication licensingATATTAGAGTTGTTGGAATGGAAGAAACGAATGAG factorGCCAATTCTCGAGGGTCAGCCAACTTCACAGTAGA TGAGAAAGAAGAATTTCAGAAATTTGCATCCGATAAGGATGCTTATGAAAAGATATGCTCAAAGATTGCT CCCTCAATATTTGGGCATGTTGATGTAAAGAAAGCTGTAGCATGCCTTTTATTTGGAGGGTCAAGGAAGTT CTTGCCCGATGGTGTAAGATTA MT113bGATCTACCAA CCTGAAAATC TGACGCATCC putative protein SEQ ID N° 367CCCCATGCTG CCCATTCAGG CATAGGCCTG At1g07990 [A. TCTCCAAAGG GGTCATCATTGTCTGACGTC thaliana] TCAAAGCGGG AGAAACCCAT GTCATCTGAC ACACCTGCTTTCTCGCTTTG TTGACTGAAG TTACCACCAT TGACAGAATC CAATCCACTA ACAGCATTTGAAGTAGAAGT GGGTGTTGCA TCTGTAGAAT TTTTGCTCTC AGCCAATTCG TCCTCCTCTCCTACTACTAC CTCGTCATCA CTATTGCTAT TCCCATCATT TGATGTTCCA TTA MT113cGATCTATCAGCAAAAGGAAATCTCTTGTGTATGTTT putative protein SEQ ID N° 368ATACTTATAAGATTCAAGATGCTGATCTATTGCAAG AT3g10420 [A.TTGCAACTGTTATGGGGCTTGACGAAGAAGTTGAA thaliana]GTAACAGATGATATTGGTATTGCGGATGCTATTCTA GCATCTAGTGCTGAAATGAAGCAGAATCCTTGGATTCGTAGTGTTGCCAAATCTCATCAAGTTTCTGTCTT TGTTGTAAAGTCAAGTACCATGGCCCAAATGGTGAAAGCTATCCGTATGATTCTTGGAATGGATTCCATTC ACTCAAAACAGCCATTA MT114TAATAACAACGAGAGCAGTCACATCATTCATGTTC 26S proteasome SEQ ID N° 369CTGCTGGTCCTAATGCTCTCTCTGATGTGCTTATAA regulatory subunitGTACTCCTATTTTCACTGGTGATGGTGAGGGTGGAA S5AGTGGATTTGCAGCAGCAGCTGCAGCGGCTGCCGCT GGTGGAGTGTCTGGGTTTGACTTTGGTGTAGATCMT115b GGGATGGAGAGAAATTTTCTCAAGTATGTGTACTGG fatty acid SEQ ID N° 370TCAAATGGTAAGGAGACAGATGATCCAACTGCGAA hydroperoxide lyaseTGATAAACAGTGTCCTGGTAAAGATC MT202 ACCCCGGCTCGAACAGGAGGAGTACGCCATGCTAAputative protein SEQ ID N° 371 TGTGCCTTGGATGATCCACATATAAAGGTCAGGCG rps12[Oenothera CCGATGAGCACATTGAACTATCCATGTGGCTGAGA elata subsp.GCCCTCACAGCCCAGGCACAACGACGCAATTATCA hookeri]GGGGCGCGCTCTACCACTGAGCTAATAGCCCGACG TGCGAGCCTCCCACTGGGGGCCCGCTATGCCAAAAGCGAGAGAAACCCCATCCCTCTCTTTCCTTTTTTCG CCCCCATGTCGCCACACGGGGGGAACATGGGGACGTAAAAAAGGGGGGCCTATCAACTTGTTCCGACCTA GGATAATAAGCTCATGAGCTTGGTCTTACTTCACCGGCGAGAAAGGAAAGAAGACTTCCATCTCCAA MT203aGATCTCCATCCAGTAATTGACCTCAAAATGTAAGC maturase-like SEQ ID N° 372CCAACAAAAAAAAAAAAAAAAACCTTGCCCCTCAT proteinTAACCCTCCAAATTGGGGAAATAACGGGGGGCGGG ATTTTCCTCACAGTGGTCACTTGAAAATCCAAAAAATGGCCGATCGGGTGTACCTAAAAGGGGGATAATG TCGGCCTACCAGGCATGTGTTGGCTAAGTTCCCTTTTCACATGAAATCCCATTCTTCATACCCTTCTTTTGCTTTTCCCACAGTTTCATAATTGGCCTTATAAACATGTTTTTGTTTTTTTTTTGCCCCGGCTTTTTTTTAACTGG CATGGGCTTCCTTTTT MT203b TAAACGAAGATGAGAAGAAA CTGTAACTTG putative protein SEQ ID N° 373 GAGCTCACGCTTATGCCTCC TTCTTTTTCT At2g34600 [A. TTTTCTCCTA AGAATTGCAC TACCCCTTACthaliana] TTCTCAACGG ATAGGGAGGA TAAAGAAAGC ACAGAAGAGA AACAACCACAGCAGCTAACA ATATTTTACA ATGGAAAATT TGTGGTTTCT GATGCTACTG AACTTCAGGCTAAAGCAATA ATATATCTGG CAAGTAGAGA AATGGAGGAG AAAACAAAAA TCCGGTCACCAATTTCAGAA TCATCATCAC CAATTTCAGA GCCTTTCATCA CCATTTTTAC AATCTCCAGCTTCTGATCTTT TCTATGAAGA GATC MT204 TAAAGTACTA ATTCCTATTT ACAATGCTCAprotein kinase-like SEQ ID N° 374 CTGCAGTATT TCTGAGCAGG CTCTTTTCTAprotein ATTTAGTATC AGCTGAGTTT TTGCTTATGT TTACTTTTTA CTCAGGCAAGGTTCTTCTTT CAACAATTGA TATCAGGGGT TAGCTACTGC CATTTC MT205 TAAACTCGGCACCTCCACCA ACTCCAAGTC allene oxide cylase SEQ ID N° 375 ATTTTACTGCAAGAGCCAGA GCGGCTCAAC TGATTCCTAA ACAACTAAAG TTCAAGAGGT AAGTG MT207TAACGTGACG GATTCGCAGC TGTACGATCT poly(A)-binding SEQ ID N° 376GTTCAACCAA GTCGGTCAGG TTGTTTCGGT protein TAGGGTTT MT208 TAAGCACATAACCTACCTTA TTGAGCAGAA 60S ribosomal SEQ ID N° 377 CAAAGCACAG TTGGTGGTTATTGCTCATGA protein L7A TGTGGACCCA ATAGAGTTAG TCGTGTGGCT GCCAGCATTGTGCAGAAAGA TGGAAATTCC GTACTGCATC GTGAAGGGAA AAGCACGTTT AGGATCGATCGTGCACAAGA AAACTGCTTC GGCTCTATGC TTGACAACTG TGAAAAATGA AGATAAAATGGAGTTCAGCA GAATTTTGGA GGCAATCAAG GCAAACTTCA ATGACAAGTA TGAGGAAAACAGAAAGAAAT GGGGCGGTGG TGTCATGGGA TCCAAATCAC AAGCCAGAAC CAAGGCGAAAGAGAGGGTTC TCGCCAAGGA AGGAGGACAG AGAATGAACT AGAGCTTCTA TTTTATGTTGCTGTTTGGGT TAGACCTACA AATTTTGTGT TTTTGATTCG C MT209b TAAGGTTCGATGACGCTAGG ATTATAAGGA Tyl-copia-like SEQ ID N° 378 AGATTTGTAT GTTATTACCGAATGTTGTTC retrotransposon CGAGTCCCGG ATGAGATC MT210aGATCTCGTCGCCTTCCACGTCTATTCCTTCAGCTGT putative protein SEQ ID N° 379TTCCTCTTTTCTAGCCTCATTGCTTTGTGCCTTA AT5g05950 [A. thaliana] MT211TAATCGTGGA ACAGGTCAGA TTATTCCAAC translation SEQ ID N° 380 TGCACGACGTGTAGCCTACT CTTCTTTCCT Elongation Factor TATGGCGACA CCCAGGCTTA TGGAACCTGT2-like protein GTATTATGTG GAGATCCAAA CACCCATGGA TTGTCTCTCT GCTATATACACCGTGTTGTC TCGCAGGCGT GGACATGTTA CTGCTGATGT TCCTCAACCT GGGACACCTGCCTACATCGT CAAGGCATTT TTACCTGTGA TCGAGTCCTT TGGTTTCGAA ACCGACTTGAGGTATCACAC CCAAGGGCAG GCGTTTTGTC TTTCAGTGT MT212 TAATCAGACT AGTGTCCGGGACCAGGTCCT lipase-like protein SEQ ID N° 381 TGAAGAGGTA AAAAGATTGGTTGAGGAATA TAAGAATGAA GAGGTGAGCA TAACAGTAAC CGGCCATAGC CTAGGTGCATCACTTGCAAC CCTAAATGCA GTTGACATAG CTTTCAATGG AGTCAACAAA ACAAGCGAAGGCAAGGAATT TCAAGTGACA GCTTTTGCAT TCGCAAGT MT214a ACAACTGTGT GGATTGTTTTAGCCCAACCC putative SEQ ID N° 382 TGTTAT phytosulfokine peptideprecursor MT301b TAAAGTCCCTGTCAGATATCTGAAGGAAGATAAAC putative GDP- SEQID N° 383 CTCACGGGTCTGCTGGTGGCCTTTATTATTTCAGAA mannoseATTTGATCATGGAGGAACTTCCGTCTCACATTTTTC pyrophosphorylaseTGCTAAACTGCGACGTGTGCTGCAATTTTCCACTGC CAGAGATGCTTGTTGCCCATAGAAGATATGGTGGAATGGGTACATTGCTAGTTATCAAGGTTTCGGCTGAA TCAGCCAACCAGTTTGGAGAGTTGGTTGCAGATCMT301c TAAAAACAGG TGCAAGCATC CCATAGTGAT putative protein SEQ ID N° 384TGTAGTTGAG ATGGACCGCA TATTGCGGCC At1g19430 [A. TGGTGGTTGG GCAATTATACGTGACAAGGT thaliana] CGAAATACTT GATCCGCTAG AGAGTATACT GAGAAGCTTGCATTGGGAGA TACGAATGAC ATTCGCAAAA GATAAGGAAG GCATCCTTTG TGCACAAAAGACCATGTGGA GACCTTGATG AATGGAGCAA ATCTTTCGCT TTCCATTTTC CAGATC MT302aGATCTAATAC CAGTATTCAG TTGTGGAAGT calmodulin-like SEQ ID N° 385AATCTCTTCG AGATTC protein MT302b ATGGTAAGGAGACAGATGATCCAACTGCGAATGATdivinyl ether SEQ ID N° 386 AAACAGTGTCCTGGTAAAGATC synthase like proteinMT303 CTTATTATGCTTTTGCTCGTTTA SEQ ID N° 387 MT305a GATCTGGTAA TTTTGGAAGGGATGGGCCGA putative protein SEQ ID N° 388 TCTTTGCATA CCAACTATAATGCAAAGTTC C42D8.3 AAATGTGATG CTCTAAAGCT TGCTATGGTG [CaenorhabditisAAGAATCAGC GGTTGGCACA AAAGTTGGTT A elegans] MT305b GATCTGTACA TGTCATCGACATTACTAAGA putative protein SEQ ID N° 389 GTTGCTGGTG AACACAACTCTGTTGTAGCA At2g32340 [A. GTTGTTGGGA AGGGTCACCT GCGTGGAATC thaliana]AAGAAGAACT GGAAACAACA CATTGAGGTT A MT306aTGCGTCTGGCTATGGAAGTTTTGGACCATCTTCTTG polygalacturonase SEQ ID N° 390GTCAAACATTTTCGGGTTGTGGCAAACATTCTTCAG inhibiting likeTCACCTGAGAAATCTAGTGGTTTTTGAAATGATAGT protein TAACCTTGTAGTTACTCAAGACTCAMT307a TAACTGAACT TGGATTTTCG CAAGACGGTT hypothetical protein- SEQ ID N°391 ATCAGTTATT TTGTGATAGT CAAAGTGCTA common tobacco TCCACCTTGCGAAGAACGCC TCATTCGATT retrotransposon CCAGATC Ttol MT307aTAACCACACCCCAAATAGACCCGTCATTCTTCAAC ambiguous hit SEQ ID N° 392CAGCACCCCACCCCCGAGTCATCTCCTTCGTCGAAC CTCCAGCGACCACTCCCTGAGCCAGATC MT308aTAAACAGAAG ATAGCTGATG AAATACTAGC putative protein SEQ ID N° 393AACTTTGAGA GGTGTCAATG TCGGTGACAA AT4g09810 [A. TGCAACAGCA CAACGAGGTGATTATGCCTT thaliana] CAGTTTCAGA GTAAATTGAT CGATATTGGG CTATCGATCAATATGCCTTC AGTTTCAGAG TA MT308b TAACCGGGATGCATTTTGCCACAACAACCTTGATGputative protein SEQ ID N° 394 ACTATTGTTCTTAGGTGGCTCGGATACATCCAAGCTAt4g33380 [A. TCTCATTTACCCCTTCCAGATC thaliana] MT309 TAAGGCACCATCAGTTTTTG ATATCAAGAA 40S ribosomal SEQ ID N° 395 TGTTGGCAAA ACCCTCGTTACTAGGACTCA protein S3a GGGTACCAAG ATTGCTTCAG AGGGCCTAAA GCATAGAGTATTTGAAGTGA GTCTGGCTGA TCTTCAAAAG GATGAGGATC AGGCTTTCAG GAAGATCAGGTTGAGAGCTG AGGATGTGCA AGGAAAGAAT GTCCTCACAA ACTTCTGGGG GATGGATTTCACAACAGACA AGTTGAGGTC ACTGGTTCGC AAATGGCAGA CTTTGATTGA GGCCCATGTAGATGTCAAAA CTACAGACAG CTATACCTTG AGGATGTTCT GCATTGCTTT TACAAAGAAGCGTCCAAACC AGCAGAAGCG TACGTGTTAT GCTCAGAGCA GCCAGATCCG TCAGATC MT311aAGAACCTAACAATCTTTACAACCTTCACTCTTACAA putative ribosomal SEQ ID N° 396ACACTCTGGGCTAGCAAACAAGAAAACTGCGACTA protein L28TCCAGGCTGAGGGGAAAGATAACTCTGTGGTGCTT GCCACATCGAAGACCAAGAAGCAAAACAAGCCTTCAACTTTGCTGAACAAATCTGTGATGAAGAACGAAT TCCCCAGAATGACCAAGGGTGTAACCAACCAGGGTGCAGACAACTACTACAGGCCAGATC MT311b TAAGGATAGG ATTGGTTATA GTATGATTACcytosolic cysteine SEQ ID N° 397 GGATGCTGAG GAGAAAGGCC TGATCAAACCsynthase TGGCGAGAGT GTCCTCATTG AACCTACAAG TGGAAACACT GGAGTAGGATTGGCATTTAT GGCTGCTGCT AAAGGCTACA AACTCATCAT AACGATGCCT TCTTCAATGAGTCTTGAGAG GAGAATTATT CTGCGTGCTC CTGGTGCTGA GTTGGTGCTT ACAGATC MT401CTGAGTAAAG GGAATCAAAT ATGAAGCAAA probable glutathione SEQ ID N° 398GGAGGAAAAC TTATCTGATA AAAGCCCTTT S-transferase PARA GCTTCTGGAGATGAACCCTG TTCACAAAAA GATCCCTATT TTGATTCACA ATAGTAAAGC CATTTGTGAGTCTCTAAACA TTCTTGAGTA CATTGATGAA GTCTGGCATG ACAAATGTCC ATTACTTCCTTCTGATCCTT ACGAAAGGTC ACAAGCCAGA TTCTGGGCCG ACTATATTGA CAAGAAGATATATAGGACAG GAAGAAGAGT GTGGAGCGGT AAAGGTGAAG ATCAAGAAGA AGCAAAGAAGGAATTCATAG AAATACTCAA GACTTTGGAA GGAGAGCTTG GAAATAAAAC TTACTTTGGTGGTGATAATC TGGGTTTTGT GGATGTGGCT TTGGTTCCCT TTACTAGTTG GTTTTATTCTTATGAGACTT GTGCAAGCTT TAGTATAGAA GCAGAGTGTC CAAAGCTGGT GGTATGGGCAACAACATGTA GGAGAGCGAG AGTG MT402b CAAAATCCAGCCCCATAACTCCACCACGTATTCGAmyocyte enhancer SEQ ID N° 399 GTCTTGCCGACGAGCTTTCCGTTAGTAGATC factor 2Alike protein MT402c TGTACACTGGTCAGTTTATTTACTGCGGTAAAAAA 60S ribosomalSEQ ID N° 400 GCTAATCTAATGGTGGGTAATGTGTTGGCACTTAG protein L2 ATC MT403aTAAACAGAAGATAGCTGATGAAATACTAGCAACTT putative protein SEQ ID N° 401TGAGAGGTGTCAATGTCGGTGACAATGCAACAGCA AT4g33380 [A.CAACGAGGTGATTATGCCTTCAGTTTCAGAGTAAA thaliana]TTGATCGATATTGGGCTATCGATCAATATGCCTTCAGTTTCAGAGTAAATTGATCGATATTGGGCTATCTTTGTTTCTGAAGCTGCATTGTTGAATCTTTTCATCGGATATCCTTCTTGTTGTTCATTCTGTAGCCTAGCTAATTGTGGACTTTCTATTATCGTGTCTTTTTCGTAATATTG CAAGATC MT403b GATCTTGGAGATGGCTTCAT GCAGCGAAGA GPAA1-like SEQ ID N° 402 CCGTGTTTAT TGTCCACTTTTGGGGTGCCG protein TTGTAACATT GCTTCCGCAC TTTCTGTCTC TAGTACCAGATTCCGCACCT CTGACCAACC TCATAACCTG GATCATGCTT TCAGCGTCCA GTCTCTTGATCTGACAAGTG ATTCTGGGTT CCTCCTTGAG TCTTCCATCC ATGACCCATA CTCGAGGAATGGAATGGACT CTTTTGAAAT CAGTGACAAT TGCTGGTGCC TGTACTGGAC TTTGCATAATGTCAGTC MT407 TGAGTCTTGA GTAATGCATA TATATAGCAC probable glutathione SEQID N° 403 AGGAAGAAGA GTGTGGAGCG GTAAAGGTGA S-transferase PARA AGATCAAGAAGAAGCAAAGA AGGAATTCAT AGAAATACTC AAGACTTTGG AAGGAGAGCT TGGAAATAAAACTTACTTTG GTGGTGATAA TCTGGGTTTT GTGGATGTGG CTTTGGTTCC CTTTACTAGTTGGTTTTATT CTTATGAGAC TTGTGCAAAC TTTAGTATAG AAGCAGAGTG TCCAAAGCTGGTGGTATGGG CAAAAACATG TATGGAGAGC GAGAGTGTCT CAAAGTCCCT TCCTCATCCTCACAAGATC MT409 GGATGGAGAGAAATTTCTCAAGTATGTGTACTGGT allene oxide SEQ IDN° 404 CAAATGGTAAGGAGACAGATGATCCAACTGTGAAT synthaseGATAAACAGTGTGCTGGTAAAGATC MT410a CTGTGTTTTA TATGTTCTTT GAGCAATATCputative protein SEQ ID N° 405 TGCAGCATAT GGAGGACAGC CCTAATTA At1g42470[A. thaliana] MT410c TAATCTGGAT GCAATTGAAG CCCTTGCCAC putative NADH- SEQID N° 406 GGACAACATT GTGTCAAAAG ATGCTTTGAC ubiquinone TTTTGAAGATCACTTCGCAG T oxireductase T1 GATCTCAGAAGTTAGGACATACGTTCCTAACGTTGTlipase-like protein SEQ ID N° 407 CGCTGGGATTATGAGAGGCATCAAAGATGTGATTCAGCTCGGAGCCACGCGCTTTTTGGTTCCAGGAATTTACCCACTCGGGTGCTTGCCGCTTTATCTCACATCAT TTCCTGACAATAATACAGGCGCGTACGATCAAATGGGTTGCTTGAGGAACTACAACGAGTTCGCTTCGTAT CATAATAGATACGTGAGCAGAGCTATCGCGAT101 ATCCAGACAAACGACCTGAAATGGATGAGGTAGTG kinase like protein SEQ ID N°408 AAATTGGTGGAAGCAATTGACACGAGCAAAGGAGGAGGGATGATACCCGAAGACCAAGCTGGTGGCTGT TTCTGCTTTGCTCCTACCAGGGGTCCATAATCTCTCTTTACTATATTTTTCTTTAGCCCCGTTGGATGGTTACT TAAGACTCAT T103 TAAGGATGTCAAAGGTTGTG ATGATGCTAA cell division protein SEQ ID N° 409 GCAAGAGCTTGAGGAGGTTG TTGAGTACCT FtsH protease-like CAAAAATCCT GCTAAGTTCACTCGGCTTGG GGGAAAGTTG CCGAAGGGCA TTCTTTTGAC TGGAGCTCCT GGAACAGGAAAAACCCTCCT TGCCAAGGCT ATCGGTGGAG AAGCAGGGGT GCCTTTCTTT TATAAGGCAG GCTCT104 ACTGGGAAAAAACCTGATCTATTGATTCAGCTTCCT heat shock protein SEQ ID N°410 AATCCACCGAGGAGTCCTGCTGCTCAAGCAGTGAA 101AAAGATGAGGATTGAAGAAATAGTGGACGATGAT GAAATGGAATACTGCTGAGGCCGTAAAATCACTGGGGTAAAATGAAGAGAAGAATACTTCACTTA T106 TTCGGCGAGA TGTTGATCAA TTTCGTACCGfructokinase SEQ ID N° 411 ACGGTCTCCG GCGTTTCCCT TGCCGAGGCT CCGGGGTTCTTGAAGGCTCC GGGCGGTGCA CCGGCAAACG TCGCCATCGC AGTGACTAGG CTGGGGGGAAAGTCGGCGTT CGTCGGGAAA CTCGGCGACG ATGAGTTCGG CCACATGCTC GCCGGGATACTCAAACAAAA CGGCGTCCAA GCCGACGGGA TCAGCTTCGA CAAGGGCGCG AGAACGGCGTTGGCGTTCGT GGCTCTACGC GCCGACGGAG AGCGTGAGTT CATGTTCTAC AGGAATCCCAGTGCCGATAT GCTGCTCACT CCCGACGA T107 TAAACCCAGA GACCTACCAA CTTTTTGACG5′-adenylylsulfate SEQ ID N° 412 CAGTAGAGAA GCACTATGGA ATCCGCATTGreductase AGTACATGTT CCCTGATGCA GTTGAAGTTC AGGCCTTAGT AAGGAACAAGGGCCTCTTCT CTTTCTACGA AGATGGCCAC CAAGAGTGCT GCCGTATAAG GAAAGTTCGACCTGTTGAGG AGAGCACTCA AAGGCTTTAC GTGCGTGGAT CACAG T109AGCTCTCTGGGTCCCTACCGACGCTGAGGTGCGAA small subunit SEQ ID N° 413AGCATGGGGAGCGAACAGGATTAGATACCCTGGTA ribosomal RNACTCCATGCCGTAAACGATGAGTGTTCGCCCTTGGTC TACGCGGATCAGGGGCCCAGCTAACGCGTGAAACACTCCGCCTGGGGAGTACGGTCGCAAGACCGAAACT CAAAGGAATTGACGGGGGCCTGCACAAGCGGTGGAGCATGTGGTTTA T112 GATCTTACTGATGATATTGTTTCTGAATATAGGAAC anionicperoxidase SEQ ID N° 414 AGTCCTCGCGCATTTGCCTCTGATTTTGCTGCTGCTATGATTAGAATGGGAGATATTAGTCCCCTAACTGG TCAAAATGGGATCATAAGAACTGTCTGCGGCTCCCTAAATTGATCATTCAAAAGCTTATTACATGTATTTT GTATTTATTTGATTCTTTA T113aGATCTCATGG CGAAAGCAAG CTATGTGCTT putative protein SEQ ID N° 415ATAATTGTAT TGGTGTATTC TGACATACCG At1g57600 [A. CGGATTGAAG TTGTTCTAATTTTATAGGAA thaliana] CTATGATTTG ATTTTAGGCA TTTGTAACTG GAGAAAGATGAATTGTATAA ATAATAACTT CAGCTGGAGC TCGTATCATG TATCATTTA T113bGATCTATGGTTTTGTCTTGGAACTCAAGCACAAGCT auxin-regulated SEQ ID N° 416TGGTCTTGCTTGAACAAGAAACACTTCTTACCTACT glutathione-SGCAGAAACCAATCATGTCCTTCGTCCCTAGTTGTTC transferaseAAGCATCAATTTATCAATATTGTTGCTACTCTGTCT ATAAATTTTATGGTTTGGTGTAATTTAGTCTTTAT116a GATCTACAAGGGATTTTGGTGAGAGTACAAAAGGA putative protein SEQ ID N° 417GATGCATGCATCTTTTGTGTGGCTATTTCAGCAAGT At1g07280 [A.ATTTTCACATACACCTACTTTA thaliana] T116b GATCTACAGG ATGGTTTTGG CAAATCATGGputative protein SEQ ID N° 418 AACTCAAAGT TATTGTCAAA GATTATCAAGAT3g58130 GAGGAAATTG CCAATTGTGA TATTTGATTG GTTTA (permease-like) [A.thaliana] T117a GATCTACGAA GCCTCTATTG AATGTTATAT zinc finger-like SEQ IDN° 419 GAACTGAAGT ATGATGTTCT TGCTTTA protein T119ACATTCTGAGAATGTTGAATTGGATAAAGTGAACC galactinol synthase SEQ ID N° 420TTGTACACTATCGTGCAGCGGGATCAAAGGCATGG AGGTACACAGGGAAAGAAGAAAATATGCAAAGGGAGGACATAAAATTACTGGTGAAGAAGTGGTGGGAC ATTTACAAGGACGAATCATTGGACTACAAGAATGCGGGTGCTGTTA T12 GATCTCATAA GTCGATTGCC AACTTTCAAA zinc-finger like SEQ IDN° 421 TACAGGACCG GATTCTTCTC GAAGAAAAAG protein AAAATGGGAG AGTGTGTTATATGTTATGCT GCATACAGAA GCGGAGATAT GTTGACCACT TTACCTTGTG CACACATGTTTCATTCAGAA TGTATAAACC GCTGGCTTA T121 GATCTACATT AGTAACCCTG AGATCACAGTputative peroxidase SEQ ID N° 422 GCCCAAAAAA TAGCAAAATC GATTCAGCTGTCTATTTCTC ACCAGGATAT GGTTCTAACT ACACATTCTC CAATACATTC TATGAAAAAGTTGTTGCTCA CGAATCTGTT CTTAGAGTTG ATCAGCAACT ATCATATGGA GCTGACACAAGTGAACTAGT TA T123 GGCACTCTCACAGAATTCTGCACCTCATCAAGCTGT Mobl-likeprotein SEQ ID N° 423 CCAACAATGTCTGCAGGGCCAAAGTCCGAGTATCGTTGGCCTGATGGAGTTA T124 TAACAGAAGC GCGACATTTT GGACACAAGA receptor-likeprotein SEQ ID N° 424 TTTGACGAAC CATATTACTT GGACAGGTTC CATATCCAAATACAAGTGAA CTTCTCTATA CAGCT T126 TAAGACAAGT CTTAGTGGAT CATGCCCTATphenylalanine SEQ ID N° 425 CGAATGGCGA CATGGAGAAG AATTGTAGCAammonia-lyase CTGCAATTTT CCATAAAATC AGTGCAGTTT GAGGAAGAAT TGAAGATTGTTTTGCTTAAG GAAATGGAGA GTGCTAGATG TAAGTTGGAG AACGGCAAGC CCACAATT T13GACTGCGTAGTGATCTCAGAACCAGTCATTCTGTGT methionine S- SEQ ID N° 426TGCTTTGCTTGGAGGATTGTATCTGAAGATGCTTAC methyltransferaseAGCTGGAATTAGTTTTGGATTTTCTGCCTCTAGACCA TCCTGCTTTA T130 TAAGGTTGAGTGCACAATAC CAAAGGACGA NADH-glutamate SEQ ID N° 427 TGGCTCGTTG GCAACTTTTTTGGATTCAGG dehydrogenase T133 TAATCGGGAA ATAATGGCAG ATGCTGAATARNA-binding SEQ ID N° 428 CAGGTGCTTC GTCGGTGGGG GAGCATGGGC proteinTGGCACCGAC CAAACACTTG GGGATGCTTT TTCTCAGTAC GGTGAAAT T139CAGTCAGGGGGGCATGGCTACAATGTCCCGGAGAA putative global SEQ ID N° 429GAGTATGCTTTAGAAGTGAAACTATCAGAGATGCCG transcriptionGGAAGTTTACCTGTTGCGGCTCAGGCTCCTGTATCTG regulatorCCATGGCTTTTCAAAGAACATCATTTGAAACAGCTTAGAGCACAATGTCTTGTGTTTTTGGCTTTTAGGAATG GTTTA T14 GATCTCAATC AGAGAGCAATGGCACGTTTC glutathione S- SEQ ID N° 430 TGGGCTAACT TTTTGGATGA AAAGTGTTTGtransferase CCAAAGATGA AGGAACTTTG TTATGAAAGC AACAATGAAG TAAGGGAGAAAGCCAGGGGA GAACTTCATG AACTCCTTA T141a GATCTAGCAT GTGTCACTTA TTTGTATTTGGTP-binding SEQ ID N° 431 TCTCTAGACC TATGCAATTC AGCAGTTCTC proteinCTTTTGGGGA ACAACTCTTC TAAGCGCATA CTATCAGTTG ATTTC T142CCCATCGTCGAATTGTCCATGCTGCTGATATGACT L-aspartate oxidase- SEQ ID N° 432GGCAGAGAGATTGAAAGAGCCTTATTAGAGGCAGT like protein GTTTA T144GACACCATTGCTTTTTACAGAGTGCAGTGTCATCTG putative SEQ ID N° 433CAAAATATTTCATTCGACACGTTTCAAATCAAAAC cyclopropane-fatty-ACCCTGACTCGAGCTCGTCGGAACATCTCTCGTCAC acyl-phospholipidTATGACCCGAGTAATGAACTCTTCTCGCTATTCCTA synthaseGATCAGACAATGACATACTCATGTGCAATTTTCAA GAGTGAAGAGGAAGACTTGAAAGTTGCACAGGAGAGGAAAATTTCTCTTCTCATTGAAAAGGCAAAAGT TAGCAAGGAACACCACATTCTAGAGATAGGATGTGGTTGGGGAAGTTTGGCCGTGGAAGTTGTTA T145 GATCTAGTGT CGTGGTCCCT CGGAATTTCAputative E2 SEQ ID N° 434 GATTACTTGA GGAACTTGAA CGCGGTGAAA ubiquitin-AGGGTATTGG AGATGGGACC GTGAGCTATG conjugating enzyme GGATGGATGATGGAGATGAT ATTTATATGC GTTCCTGGAC TGGCACCATT ATTGGTCCTC ACAATTCCGTTCATGAAGGT CGCATTTATC AGTTGAAGTT ATTCTGCGAC AAAGATTATC CAGAGAAGCCACCAAGT T146 AATGGGTGCAAGTGTGGATCAAACTGCACCT putative type II SEQ ID N°435 metallothionein T147a TAACATAAAACTAAAAACAGATAAGGTTCATATCA putativeprotein SEQ ID N° 436 CACAAGCAAGAAATCCCAAAAGGAGGGTTCACCTC OSJNBb0072E24ACAAGTATAACAAACTTGAACATACAATTCCAAAC [Oryza sativa]ACTTGCTTTCTTTCAATCATTCTTGCCTGAAACATTT CCAGGAACATTCAAAACACTAGATC T148CTTATTATGTGGACAATTCTGAACCACAGTGGACA putative membrane SEQ ID N° 437CCTTGGTTGGTTCCAATGATTGTGGTTGCCAATGTA proteinGCCATGTTTATTGTAATCATGTTCGTTACTCA T149a GATCTAGGTA CATTGAGCTA TTTCCTTCACribonucleoprotein- SEQ ID N° 438 AGCCAGATGA AGCTAGACGA GCCGAGTCAA likeprotein GGTCACGACA GTGATGCTAA TTATTTCTGG CGGAGCATTT TTAGGCATCATATATTTCGT CCACCTCTTC TCTTGGGGAT ATTGTAGCAG TTGTT T150 GATCTAGGAAGAGAGAGAGA GAGGGAGCTG serine/threonine SEQ ID N° 439 ACCCATAACTCAGGCAGTTG ATCGGAAAAG protein kinase AGATGGGGTG GTCGTTCTCG GGGTTGAATGCTTTATGCGA CGCCGTTA T151a GATCTAGACA GAGAGGGCAG CCAACTTCAAprohibitin-like SEQ ID N° 440 CATTGCTCTA GATGATGTGT CCATAACAAG proteinCCTGACTTTT GGAAAGGAAT TTACAGCTGC AATTGAAGCA AAACAAGTGG CTGCTCAAGAAGCTGAAAGA GCAAAGTTTG TTGTGGAAAA AGCTGAGCAA GATAAGCGAA GTGCTGTTATCAGAGCTCAG GGTGAGGCTA AGAGTGCCCA GCTTATTGGT CAAGCGATTG CCAATAATCCGGCATTTCTC ACACTCAGGA AAATCGAAGC AGCAAGAGAG ATTGCCCAGC CTCTCTCACATGCAGCAAAC AAGGTGTACT TGAG T151b GATCTAGGAA ACTTTCCCGT CACTTTTTTGambiguous hit SEQ ID N° 441 CCCAAATTCT TGAAGCTCCA ACCACTACCA CCTCACAATACTTATATCAA TGGATAGAGC TCCTCAAGAC CTAGCTATTG ATGCCAATTT TACCATGAAAATCCGGCGAT CAAAATCCGG CCAAATTCCG GCGACCTCCC CGAACACCCT CTTTTGGCATACCACCATTT TTTCGGCCAC TTGAATTATA AAATGGTAAT TTTCGGACCA TGTAAACTCATAAAATCGAG TTGGAATGAA AGATAATGAC GCTGAGAAAT ATTAGTAGCT T153 TAAGCATATAGCTTTTCCTT CTGAGCCAGG lectin-like protein SEQ ID N° 442 ATCACACTTCACACTAACCG AATCTCGCAT AGAATCCATA AATGAAGAAA GCATCTCAAT TGGAGAAAAGTTTGTTTTCC CGGGGAATTT GCTTGTCAAC GAAATTCCAC TCATAAGTAG GTTCACATCGTGATCTAAGT TCCATTTCCC ATCGAGAGGT GAGTGATACT GGTAGGAGAG TCCTATTCTTCTCCTTGGGT TATGAAAGAA TTCAATAGCT CCGGGCTCCC TCACTGC T154 TAAGGCTGCCTACGAAGCAA TCTCAGATTT putative reverse SEQ ID N° 443 TACATGCAATAAAAAAGACT ACTCTTGGCT trancriptase CTGGAAAATC AACTCCCTAA ACAAATTGAAATATCTCCTC TGGACAATCA TTTGGGACAG GTTACCCACA AAGCATATGG GGGCCAAAAGAGGGATTTGC CATGACGACA CTTGTAACAT ATGTAATAGG GAGCCTAAGA ACATAGAACA T158GGGCAAACGTGCTGGGAATAAATCTGAATGTGCCA glycine-rich protein SEQ ID N° 444CTCTCTCTTAGCCTTGTTCTCAACAACTGTGGAAGGAATCCTCCTACTGGCTTCACTTGCTAAGCGCAAGTA CCCGATTA T160b GATCTCTTGCCTCGTGCAGA CATGCTTGAT putative SEQ ID N° 445 TCTCGTCCTT TGGCCACTCCTCTTACTAGT retroelement pol GGTACCGAGC TTCCCAATGA CTGCGTAGTG polyproteinATCTAGGGCG GGTTCTGTTG ATGTGTACAT ATAATAAGAT CACATCTAGA TTATGGATTCTCTTTGAGGA TAAGTTTCAC TTTTTGTTCC TACCTTTTTG TAGTAAATTT T164AGGCTGGTACCGGTCCGGAATTCCCGGGATATCGT par peptide SEQ ID N° 446CGACCCACGCGTC CGATATTCTCAAACAAAAAGAATGGAGAGCAACACGTGGTTCTGCTAGATTTCTGGCCAAGCTCTTTTGG TATGAGGCTAAGAATTGCATTGGCCTTAAAGGGAATCAAATATGAAGCAAAGGAGGAAAACTTATCTGAT AAAAGCCCTTTGCTTCTGGAGATGAACCCTGTTCACAAAAAGATCCCTATTTTGATTCACAATAGTAAAGC CATTTGTGAGTCTCTAAACATTCTTGAGTACATTGATGAAGTCTGGCATGACAAATGTCCATTACTTCCTTC TGATCCTTACGAAAGGTCACAAGCCAGATTCTGGGCCGACTATATTGACAAGAAGATATATAGCACAGGA AGAAGAGTGTGGAGCGGTAAAGGTGAAGATCAAGAAGAAGCAAAGAAGGAATTCATAGAAATACTCAA GACTTTGGAAGGAGAGCTTGGAAATAAAACTTACTTTGGTGGTGATAATCTGGGTTTTGTGGATGTGGCTTTGGTTCCCTTTACTAGTTGGTTTTATTCTTATGAGACTTGTGCAAACTTTAGTATAGAAGCAGAGTGTCCAA AGCTGGTGGTATGGGCAAAAACATGTATGGAGAGCGAGAGTGTCTCAAAGTCCCTTCCTCATCCTCACAAGATCTATGGTTTTGTCTTGGAACTCAAGCACAAGCTTGGTCTTGCTTGAACAAGAAACACTTCTTACCTACTGCAGAAACCAATCATGTCCTTCGTCCCTAGTTGTTCAAGCATCAATTTATCAATATTGTTGCTACTCTGTCTA TAAATTTTATGGTTTGGTGTAATTTAGT T168GATCTATCCA TGGAGTGAAT TTCGCATCAG putative lipase SEQ ID N° 447GTGGAGCTGG CTGTTTA T17 GATCTCAATG GTGAATTGAC CTTGAAACAA annexin SEQ IDN° 448 GTAGTTCAAT GCCTTTGCTC ACCTCAATCC TACTTCAGCA ACATTTTGAT CGCGTCCTTAT171 ATGGACATTTGTGTACGAGAAGAAACCTGAAGAAA wound-induced SEQ ID N° 449CCCCAGAGCCTCTCGTTTTGTTGGCTTATGCCCTAC vacuolar membraneATGTGACCAAAGATGTAGAGAGTCACCTTCTCAAG protein Sn-1TAATCTAATCTATGCTATTCAATGGTTCATAGCCAT ATATATATGTATGTTA T172TGGGAGCTGAAAATGGCCTGATTGTTAGCGATAGC protein phosphatase SEQ ID N° 450ATCATTCAGGGAAATGAAGAAGACGAGATTTTATC 2CTGTTGGAGAGGATCCTTGTGTAATTAATGGGGAGG AGTTGTTGCCACTGGGCGCTAGCTCGGAGTTGAGTGCCAATTGCTGTTGAAATCGAGGGTATTGACAAT GGTCAAATACTTGCCAAAGTCATAAGTTTGGAGGAAAGGAGTTTTGAGAGAAAGATCAGTAATCTGTCCG CCGTTGCTGCTATCCCAGATGATGAAATTACTACTGGCCCTACGCTAAAGGCATCCGTAGTGGCTCTTCCGT TGCCTAGTGAGAATGAACCTGTCAAAGAAAGTGTCAAGAGTGTGTTTGAATTGGAATGCGTGCCACTCTG GGGCTCTGTATCTATCTGTGGAAAGAGACCAGAGATGGAGGATGCTCTTATGGTTGTTCCTAATTTCATGAAAATACCTATCAAAATGTTTATTGGTGATCGTGTGA TTGACGGACTAAGTCAACGTTTGAGTCACCTGACATCTCATTTTTATGGTGTATATGATGGTCATGGAGGATCTCAGGTTGCGGATTATTGCTGCAAACGCATTCATTTAGCATTAGTTGAGGAGTTAAAACTTTTCAAAGAT GATATGGTGGACGGGAGTGCAAAGGACACACGTCAGGTGCAGTGGGAGAAGGTCTTTACTAGTTGCTTTCT CAAGGTTGACGATGAAGTTGGGGGGAAAGTGAACAGTGATCCCGGTGAAGACAACATAGATACCACTAG CTGCGCCTCTGAACCTATTGCCCCGGAAACTGTGGGGTCCACTGCGGTTGTAGCGGTGATATGTTCATCTCATATTGTAGTTTCTAATTGTGGGGATTCAAGAGCAG TCCTTTATCGTGGCAAAGAAGCAATGGCACTGTCAATTGATCATAAACCAAGCAGAGAAGATGAGTATGC TAGAATTGAAGCATCTGGTGGCAAGGTCATTCAGTGGAATGGACATCGTGTTTTTGGCGTCCTTGCAATGT CAAGATCTATTGGTGACAGATACTTGAAACCATGGATTTATACCCGAACCAGAAATTATGTTTGTACCACG AGCCAGAGAAGACGAATGCCTAGTTTTAGCTAGTGACGGGTTGTGGGATGTCATGTCAAATGAGGAAGCT TGTGAAGTAGCTAGACGACGAATTCTGCTATGGCACAAAAAGAATGGGACTAATCCTCTGCCGGAAAGGG GCCAAGGAGTTGATCCTGCTGCACAAGCAGCAGCAGAGTATCTCTCGACGATGGCTCTTCAAAAAGGTAG CAAAGACAATATATCTGTGATTGTGGTGGACCTTAAAGCTCAAAGGAAGTTCAAGAGCAAATGTTAAGAG ATGACAATGTTCACCCGCACTTTGGTTTTTAGTATAAATCTATATACGGCTATGGGGTATAATCTCATTATTACATAACTCGGTCCATCCATTTTTTTATGGGCTTAAGGTCTGTGTATGAGAATAGTGTTTAGCATGTATTTA TAGAAAAACAGTTTAACAAATGACGTTTATCCAAATTTTTGGTGTTGTTATGCCAGCAAGTGGCTATGTAA ATTGAGCATGTTGTAGCAATATCAAAGATGCAAGTTCTTTGTTTAAAAAAAAAAAAAAAAAAA T177a TGACTGCGTAGTGCTCTATATGGCAATAGATTTGAAleucine-rich repeat SEQ ID N° 451 GGCAACATTCCCAAGCCTTTTGCTAAATTGAAGTCTprotein CTTAGATTTTTGCGGTTA T177c GATCTATACCAGAAGGAGCTGTTGTATGTAATGTG 60Sribosomal SEQ ID N° 452 GAGCATAAAGTGGGAGATCGTGGTGTTTTTGCTAG protein L2ATGCTCTGGTGATTATGCCATTGTTATCAGCCACAA CCCTGATAATGGTACCACTAGGGTTA T178CTGGAATCAATTGCTTCCTCTGCGGTGCGGGCAGC pyruvate kinase-like SEQ ID N° 453GATTA protein T18 TCAAAAACAA CTTTTATTGT GTTCATGGTT pathogenesis-relatedSEQ ID N° 454 TTAGCCGTGG CCCATTCTTC ATTAGCCCAA protein AACACTCCCAAAGATATCGT TATTGTCCAC AACAAAGCCC GTGCAGAAGT TGGTGTCCCA CTCCCACCAT TA T2TGAGTGAGCT TCATTATCTA CAAGCTTCCA putative cytochrome SEQ ID N° 455TTTATGAAAG TATGAGACTT TACCCTCCTA P450 TCCAATTTGA TTCAAAGTTT TGTTTAGAAGATGATATTTT ACCTGATGGG ACTTTTGTGA AGAAAGGAAC AAGGGTTACG TATCATCCTTATGCAATGGG AAGAATGGAA GAATTATGGG GTTGTGATTT T20GATCTCATTTCGATCCTCACCACCCTCATCTGGCTA 13-lipoxygenase SEQ ID N° 456GCTTCAGCACAACATGCTTCGCTGAATTTCGGCCAG TACCCATACGGCGGCTACGTCCCCAATCGGCCACCTCTCATGCGTAGATTA T201 GATCTCGCTT CGGGATCATT CCCCAAGAGC MRP-like ABC SEQID N° 457 CAGTCCTTTT TGAAGGAACT GTGAGAAGCA transporter ACATTGACCCCATTGGACAA TATTCAGATG ATGAAATTTG GAAGAGCCTC GAACGCTGCC A T203TCATCGAAATAATGAGTCACCATTGATATCGACAC chloroplast putative SEQ ID N° 458ATCTCCGATCGCCAAACGCTCGGGAGTTCCTCTAT protein 1708CAATCCTTTTCCTTCTTCTTGTTGCTGGATATCTCGT [Nicotiana tabacum]TCGTACACATATTGTCTTTGTTTCCCGGGCCTCTAG TGAGTGACAGACAGAGTTCGAAAAGGTCAAATCTTTGATGATTCCATCATCTATGATTGAGTTGCGAAAACTTCTGGATAGGTATCCTACATCTGAACCGAATTCTT TCTGGTTA T204 GATCTCGAGC TCAGATTACAAAGCAAATCA putative protein SEQ ID N° 459 AGCATTTGTT TGGCAAGGAACTAGAAATCG At3g46190 [A. GAACCGCGAA AATGACAACC TCTTGAACCG thaliana]AAACCCATTG ATAAAACCTC GACAAACCTC ACCTACCTCA ACTCCCATGC TTTATGGTTGTGTTTTTTGG TAGAAGAAAT GGTGTTTCGG AGCTAAAGTG AGGAGCTGTT TCGAACAAGGCTTCAGCTGC GTTATTGACT GATTTTTTGG TGAGTTTCGG GGTTA T205aAGATGTGACAGCCCGTTAGATTTACGTCATAAGAG putative apoptosis SEQ ID N° 460GGCTGGCGTCGAGCCGCTTGGATAGATTTGATCGA inhibitor likeCCCCAGGTGCATCCTTGGGGAATTCCTGTGTTCGT proteinCAAGGTCTAAGCCGATTTATTCCTGGCCGGACGT CGACAGGTTTTGAGGGAAGTGACTGACCCGAGATCT205b GATCTCGAAC TAGCGATCTC AAATTTCACC aklanonic acid SEQ ID N° 461TCCAGTTCCA CCGAAAATTA CCGTTCTGCT methyltransferase TGTGAAGCTA CTACTAGCACGATTCCCGAA like protein GAAGTGGAAA CCGGACTTGT TGTCGGTGGG CCCCATGGACCGCCGCCAGG ACTCGCTGGA AGATTATTAC TCTGCCGTTT TCAA T206aGCTATAAACCAGACACAAATATCTCCATCTGGGAG non-photosynthetic SEQ ID N° 462GCAGCATACCAATCTGAAGGTGCATTTCTTGACGA ferredoxinCGATCAAATGGAGAAGGGTTATTTGCTGACTTGTA TTTCATACCCGAGCATC T207b TAACGATGTCAAAAAATTTC TGTCGGAGAC phosphatidylinositol- SEQ ID N° 463 AGAATCAGAGATTATAATCC TCGAGATC specific phospholipase-like protein T208TGAGTAACGTGAGGGAAACTGCTCTTCCTTCAGTA putative protein SEQ ID N° 464ATTGCACAATACCCCGAGATC AT4g02990 [A. thaliana] T21 TATCGATTAT TCATACAGTGAGAGCATAGC cyclophilin SEQ ID N° 465 TTAAAAACTC CACAGAAATT TCTAGAAGAGAGTGAGAGAT GGCAAATCCT AAGGTTTTCT TCGACCTTAC CGTCGGCGGT CTACCGACCGGCCGTGTGGT GATGGAGTTG TTCAACGATG TAGTTCCGAA AACAGCGGAT AACTTCCGAGCACTCTGTAC CGGAGAGAAA GGCGTCGGAA AGTCCGGCAA GCCGTTACAC TACAAAGGATCATCATTTCA CCGTGTGATT CCTGGATTTA TGTGTCAAGG AGGTGATTTC ACTGCTGGAAACGGTACCGG CGGTGAATCG ATCTACGGCG CCAAATTCGC CGACGAGAAT TTCGTTAAAAAGCATACTGG ACCTGGAATT CTCTCTATGG CCAATGCTGG ACCTGGAACT AACGGATCTCAGTTTTTCAT CTGTACGGCC AAAACCGAGT GGCTTGATGG GAAACACGTG GTGTTTGGTCAAGTTATTGA AGGAATGGAC GTGATTAAGA AAGTGGAAGC CGTTGGATCT AGCTCCGGCAGGTGCTCGAA GCCCGTTGTG ATTGCTGACT GTGGTCAACT CTCTTAGATT ATTAATCGTATCAATTAATG TTAATGATGA TCTAGTCTAG TTAACTATGT GATCGCAGTG TACTGATTTGCTGGTTTTCG TTTTTTTTTT AGCCTTTTCC TTTTTGAGAT TGTGGGTCGG GTTTCGGGCGTACTGTGTCG GGTCTTTACT GTAATTGGTG GTGTTTACTA CTACCAGTGC ATGTTGGAATTGGAATAAGA TTAGATTTCT CGGTTTAAAA AAAAAAAAAA AAAAAA T210ACAGCTATGACCTTAGGCCTATTTAGGTGACACTA putative protein SEQ ID N° 466TAGAACAAGTTTGTACAAAAAAGCAGGCTGGTACC P0638D12 [OryzaGGTCCGGAATTCCCGGGATCTCAAAAAACACGATC sativa]AATGATCCGTACAACTCTCTCTTATCGAGTCCTCT ATTTCCAATAATCACCAAATTACCCCACAAGTTTTCGATTGGATCAATTTAGTGTTTGATCTTTAGCTGT TCTGATCAGTTTATTAGTGGAAATGAAGATAGTGGATTTGGATGAGTCGTTAATGGAAAGTGATGGCAAT TGTGTAAATACTGAGAAACGGTTGATTGTTGTTGGTGTTGATGCTAAAAGAGCGTTGGTCGGAGCCGGGG CTCGGATCCTTTTTTACCCGACCCTTTTATACAATGTTTTCCGCAACAAAATTCAATCGGAGTTCAGATG GTGGGATCAAATTGATCAGTTTCTCCTCCTTGGAGCAGTTCCATTTCCCTCGGATGTCCCTCGGTTGAAG CAGCTTGGCGTTGGTGGTGTAATAACACTGAATGAACCTTATGAAACTTTGGTACCATCATCATTGTACC ATGCCCATGGGATAGACCATCTCGTTATTCCTACCAGAGATTATCTTTTTGCACCCTCTTTCGTGGATAT AAATCGAGCAGTAGATTTTATTCACAGGAATGCGTCCATTGGCCAGACTACGTATGTACATTGCAAAGCC GGAAGGGGAAGGAGCACAACCGTTGTGCTTTGCTATTTGGTGGAATATAAGCACATGACTCCTCGTGCTG CCCTTGAATTCGTCCGCTCCAGAAGACCTCGAGTTTTATTGGCTCCTTCTCAATGGAAGGCTGTTCAAGA ATTCAAGCAGCAAAGAGTGGCATCTTATGCGCTCTCTGGTGATGCTGTATTGATCACTAAAGCAGATCTC GAAGGCTATCATAGTTCTTCTGATGATAGTCGCGGTAAGGAACTGGCCATTGTGCCTCGAATAGCAAGAA CACAGCCGATGATAGCTAGATTATCCTGCCTCTTTGCATCCTTGAAAGTATCAGATGGTTGTGGACCTGT TACCAGGCAACTGACCGAGGCACGTGCCTGCTAATCGCAAACTCATCAGCAGCAGCTACCTTGTACAGAA GACCACTGCTTAAATAAGGTCAGAAAGAGTCTTATATCTTTGAATCTGTGCTTCAGAGTGAACATCAAGG GATTATGAATAGAAAAAAACAGCTGAAGAGTACTTCAACATTGTGTAAACATGTTCAGAGTATGACTACT GTGGTCATTAGTAAATATTGCATAATTATACTCTTCCCATAATAAAGGGCGGGTATACAGACTTATTCTG AGAAAAAAAAAAAAAAAAAAA T211TAAGGCAGAA AATAAACTCC TATTGCTTTG beta(1,3)-glucanase SEQ ID N° 467ATGTGCATGT TACAGTATAT GTTACAAAAG regulator AAAAACTTTC TGTTTATATAGTAGGAGAGT TTCATCCCTA GTATAAGTCT AAAAAGGTAA AAAT T213CACTCTCTCTTAGCCTTGGTCTCAACAACTGTGGAA putative SEQ ID N° 468GGAATGCTCCTACTGGCTTCACTTGCTAAGCGGAA strictosidineGTACCCGATTACTCAGGACTCATCATCTACCAGCG synthaseCAGGCAATTTGTTGCTGCGACTGCAAGTGGAGATA AGACAGGCAGGCTGATGAAATATTATAAACCAACAAAAGAAGTAACAGTTGCACTAGGAGGCCTA T214GATCTCGGATTTCTTATTTCATTGCCCTCTTCCTTTA putative protein SEQ ID N° 469TTCCTCACTGGCTGTTCGTATTA P0501G01 [Oryza sativa] T216 TAAACAATGTTCAGCCTTTC GTTGCAAGTT amidophospho SEQ ID N° 470 ATAAATTTGG ATCAGTTGGTGTTGCCCACA ribosyltransferase ATGGCAATTT TGTGAATTAC CTAGCTCTTCGTGCTGAACT TGAGGAAGAC GGGGCAATTT TCAAGACTAG TTCTGAGACT GAGGTGGTTCTTCACCTTAT TGCTAGATCA AAGAAGGAGC TTTTTCTTTT GAGGATT T217 GATCTAGTGTCATGGTCCCT CGGAATTTCA putative E2 SEQ ID N° 471 GATTACTTGA GGAACTTGAACGCGGTGAAA ubiquitin- AGGGTATTGA AAATGGGACC GTGAGGTATG conjugatingenzyme GGATGGATAA TGCAAATTAT ATGTATATGC GTCCCTGGAC TGGCCCCACT ATTGGCCCTCAGGATTCCGT TGACTGCGTA GTGATCTGTA ACTGCCGAAG ATATCATCTT GCCGCCTCATGTAGAAAT T22 GATCTCACTC CAAATCACAA TCTCCGCCGT putative protein SEQ ID N°472 CTGATCCAAT CATGGTGCAC ATTA At2g35930 [A. thaliana] T220CATCCATCATTATCTTAGGTACACCCGTCCAGCCAG glucose 6 SEQ ID N° 473GCAACCCTCTTGGAGCTGCCATTGCAATTCTTGGAA phosphate/phosphateCTGTCTTGCATTCACAGGCAAAACAGTGAAGAGTG translocatorGAATTTATATATCGCGCAGGAAAGGTGTCGGAGAG AACCGAGAGGTGTTGAGAAAACGTATCCCATAATCCTGAATCTACCCTTACTTGAGGTGGAACATGAAAC TTATTAGTATGTACATAGCAATAATGGGTTACTCAAGACT T221 ACTTTGGTACTCCACGTTGTGGGACCTACTGGTGGA putative SEQ ID N° 474TTGGCTACCCCACTTGTCCAAGATTTTGAACGCCAA strictosidineCCTCTTCTCTTTCACAAATGATCTGGACATTGATGAC synthaseGACGACGATATTATTTACTTCACGGATACAAGCAC AATCTACCAGCGCAGGCAATTTGTTGCTGCGACTGCAAGTGGAGATAAGACAGGCAGGCTGATGAAATAT AATAAATCAACAAAAGAAGTAACAGTTGCACTAGGAGGCCTAGCTTTTGCAAATGGTGTAGCCTTACTCAG GACTCATCAC T222a GATCTCTCCAATTTCCTCTT CACTGTCGGT putative protein SEQ ID N° 475 GCCAGAATCCCTGCTCAAGT CTTTGGTTCA At3g56950 [A. ATTACTGGGG TTAGGCTCAT CATTGCAGCAthaliana] TTTCCAAACA TAGGACGAGG ACCTCGTTTG ACCATTGACA TCCACCGAGGTGCACTGATT GAAGGGTGCT TGACATTTGC GATTGTTACC ATTTCACTTG GACTTTCCAGAAGAAGTCGT T222b GATCTCTTGC CTCGTGCAGA CATGCTTGAT retrotransposon- SEQID N° 476 TCTCGTCCTT TGGCCACTCC TCTTACTAGT like protein GGTACCGAGCTTCCCAAGTT GGATGTCACT TCCCTCTCTG ATCCCACCTA TTTCATTCTT CTATTGAGTCGGCTAACTGT AACTATAAGC TACACGCCTC GAACTCGTAT AAAGATTCTT CCTCTAGGGCCTCCTTTCAC CTT T222c CGACAGAGAGCAGCCCTGAATCTTTGGCTATGTCA putativenucleic SEQ ID N° 477 ACTCCGTTCCTACACATTTCTCGTCCTCTTTCTCCAC acid bindingprotein ACGAGTACAACCATAAGCCTTATAAATACTGAAATCTCATCAATAGCTGTGACTTGTAATTGACTAACTAAGCCCATGGCTTCCAACTCTTCCTCCCATAGCCCTCG CACCGTCGAAGAGATC T225GTGATCTCTTGCTGTATCAAGAGGTATTGGAGATCA protein phosphatase SEQ ID N° 478GTGTCTTA 2C T227 TAATCCAAAC AAAACTTCTA CTGCGAAGAA ribosomal protein SEQID N° 480 GGTCCGCGGT GTAAAGAAGA CCAAGGCTGG S19 TGATGCTAAG AAGAAATAAGTCTTATGCAA ACAAAAATCT CAATTTGGGA TTCTTTTGGT GGCCTATGTA TTTGTCTTGTGGTACTGTTG ATTTTGACTT TGATTTTGGG GCGATTCAGT TATCTTCCCA TGGGGATATCTCATGGAAGG CTTAGAGTAC TTGAGAGTTC TATTAGTTAG T T228GGCCTTATTCTTTTGCTTTCAGGATTCATCTTACCAC putative protein SEQ ID N° 481CTACTGATGGCATCGCGCATCATCGACGATCCCTCT At5g05740 [A.GTGTTCCACGAATCATTTCTAGCTGGCGGTATAGCC thaliana]AAGCTTATTCTAGGAGATGCTCTCAAGGAAGGAAC TCCTATATCAGTAAATCCGCTTGTCATATGGGCCTGGGCTGGACTTCTCATTA T229a GATCTCTAGC ACAAAAACGA CCCCCCCCGTnucleoporin-like SEQ ID N° 482 TAGTCATCTT CTCCAGACAA TCCCTAAGTC proteinGACGAGTAGC TGCTGCCTCG TCCTTCACTG AGCATCCAAA GTCCAAACGC CGCTGCTTTCGTCTTCAACC CATCGTCCAA CTTCACGTCG T25 TAAATGGAGCAAGGCTAAGCTTGTCTGGTGATCACputative SEQ ID N° 483 CAGCTCAGTAATGCTGGCCTTGCTGTATCCCTTTGTfolylpolyglutamate AAAAGTTGGCTTAGAAGTACAGGAAACTGGAAAA synthetaseGGCTGTTTGAAGATGCATATGAGAAAGATGGTCTA CCAGAGGAATTCCTGAGGGGTCTTTCAGCTGCACGTCTTTTCTGGCAGGGGTCAGATTGTTGTTGACCCTCTGATCAACACATCTGGAGGACATAAAAGGTTGTCAG GAGATC T27 GATCTCCCAA TACTGACCGGGGGATAGGAA transposase-like SEQ ID N° 484 GTCCATTGCG AGAATATAGCCCTAATATAC protein GAGATGAACT TAGAAGACGT TATATTCAAA TGGGACCTTGCCAGCCTACG AGTCATGATT TTCCTAAAAC TAAGTTTGGG AAGACAATGC GTCAGTTTTATCCTGGTTGG TTTTA T28 GATCTCCTAG GAGTGTTAGT GACAAAGATA nicotinic SEQ IDN° 485 GCCCACGTTC TGTGTTTTTG GATCGCAGTT acetylcholine CATCGTCAAATTCTAGGCGT AGTTCTAGTG receptor epsilon GTACTAGTTC CGAAGCATCC GTACAGTAGCTTTA subunit T3 TGAACCCTTT TTGATGGACT TAAGGGAATA putative protein SEQ IDN° 486 ATTTGGTGAC CCAATCTTCC TCCTCTTGGA P0529E05 [Oryza CTTCAATTTGGACCACCATA TAATTGTAAA sativa] ATTTGGACAA TTTATTTCCT TTGGTCTTGAGCTCTTCCTC TACAATTGAA AGCTTCTTTA TTTGCCATTG AAGTCTAGCA ACCTTAGTAG GCAAT30 GATCTCCCAG AAGGGGTCCA AAGCATCATT putative protein SEQ ID N° 487GCAGATTCTA GTGAATGTGT GTCAATGGGG At2g35930 [A. GAGGAACAGA GTGAAAGCGGCGGAGACTGA thaliana] CGCGGTTAGA T302aTAAAGCCACAGACAAGACCAACTACATTGGTGCTA putative protein SEQ ID N° 488ATGATCTTCAAGCTACCTACTCTCTCTATCCAGGAA At1g76660 [A.GTCCTGCTACTACTCTCAGATCACTACGCAGTCAAT thaliana] CCCGCG T302b GATCTGGATTTACCTCCACC TCCCAGGCCT splicing factor SEQ ID N° 489 GGTTTTCCATCTGTTAGGCC ACTACCTCCA CCTCCTGGGC TTGCGCTGAA TATTCCTAGG CCTCCTAATACAGTCCAGTA TTCCACCTCC ACCAGTGCTG GGGTTGCTGC TCCACCTCGA CCTCCTATGGTTACTCAGGG GTCATCAATC ACTAGT T302c ACTAGTGATTGACTGCGTAGTGATCTGACTTGTCCGpectin SEQ ID N° 490 CTTTGTATAGATGTGAC methylesterase T303bCGAGTTGAATGAATCAAAGCAAGACGAAGTCAGCA cysteine-rich protein SEQ ID N° 491GTCCTCGCTCTTGAATCAGATC T305 GATCTGAGGAGAGTTTGCATTTTGGATTTGCGCACGarabinogalactan- SEQ ID N° 493 AGATGTTTATGATTCTAGGATTTATTTTAGTCATCTprotein TACTCGGCTGATGTTTATTCGCTTTTGTGACTTTTACTCGTGGGCGGTGGTGACCGCGTACATGCTATTTATT TGATTTTTACTATGGTTATTGTTTATTGTTAT308a GATCTGATCC AGCAGTTGTT CTTGCATTTG putative protein SEQ ID N° 494ATATTCAGTG TAATATTGAA TCATTTTATC BAC19.2 AAGTTATCGT TGCTGTCCCTTTTCTTGGTA [Lycopersicon ATCCAGTTGT CTGCTTTGAG ATTTACTCTT esculentum]CTGAATCAAA ATCTTGGAGT TGCTCTTCTT CAGACTGTAT TGAGTTGGAA AATAGCACAAGTCCTCTAAT CTTTGATA T309 ATGGGAACGGCTTCCTGGTTGCACTTGTTGGTACGG putativeprotein SEQ ID N° 495 ACTCTCTTATGGAGTTTTGACTGGTACTTCCCTTGTT 4933419D20[Mus CCCCTGTACTTATTCCTAATACTCCAGGAAATATTG musculus] CGTCACTTGGCTTA T311ATAAACAGCCTTGGATGATTCTTGCTGCTCATCGTG putative protein SEQ ID N° 496CCCTTGGTTACTCCGCTAATGATTGGTATGCTAAGG AJ271664 [CicerAAGGCTCATTTGAAGAGCCCATGGGAAGGGAGCAC arietinum]TTGCACAAACTCTGGCAGAAATATAAGGTTGATAT GGCATTTTATGGGCACGTCCATAACTATGAAAGAGTTTGCCCAATTTACCAGAATCAATGTGTGAACAAG GAGACATCACACTACTCGGGCGTAGTGAAAGGAACAATTCATGTTGAAGTTGGGGGAGGAGGAACCCTTT TGAATAAATT T313aGATCTGAGACCGGGGTTTATCGAGACTGAGTTTTAT phospholipase D SEQ ID N° 497ACTTCTCCTCAAGTGTTCCATTA T314 CTAAGGGTGCTGCCAGCTTTACCTCCCAAGTCATCAelongation factor-1 SEQ ID N° 498 TCATGAACCATCCAGGACCGATTGGAAATGGATATalpha GCTCCAGTGCTTGACTGCCACACCTTCCACATTGCTGTCAAGTTTGCAGAAATTTTGACCAAGATCGACAG GCGTTCTGGTAAGGAGATTGAGAAGGAGCCCAAGTTCTTGAAGAATGGTGATGCTGGTATGGTTA T315a GATCTATGGT TTTGTCTTGG AACTCAAGCAPROBABLE SEQ ID N° 499 CAAGCTTGGT CTTGCTTGAA CAAGAAACAC GLUTATHIONE S-TTCTTACCTA CTGCAGAAAC CAATCATGTC TRANSFERASE CTTCGTCCCT AGTTGTTCAAGCATCAATTT PARA ATCAATATTG TTGCTACTCT GTCTATAAAT TTT T315b GATCTTGATAACAAACGTAA TACTAACATG putative protein SEQ ID N° 500 AAACAAGCTAATGGAACACA AAATTTACAG At2g44270 [A. AGCAAACAGT GTGGAAGCTT GGACTTTTGAthaliana] ATCATCATAT AACTGTATAA TCGTTGTATA ATTCTCAGTG GTGATCATTG CGATCTT319a GATCTTGCCATCACAGAAAAGGATCATTCTGGGCG RNase NGR2 SEQ ID N° 501CATGAGTGGGAAAAACATGGGACATGTGCTTATCC AGTTGTCCATGATGAATATGAGTTCTTTTTGACTACGCTGAATGTTTACTTCAAGTATAATGTTACAGAAGT TGTGCTTGAAGCTGGATATGTACCATCAGATTCCGTAAGTATCCATTACGAGGCATCATTTCATCAATTGA AAATGCTTTCCATGCAACCCCA T319bGATCTCATCA TGAATGTTGG TACTGGTGGC 60S acidic SEQ ID N° 502 GGTGGTGCTGCAGTTGCTGT TGCTGCTCCC ribosomal protein ACTGGTGGTG CCAGTGCCGG TGCTGCAGCTP1-like protein GCTGCCCCTG CTGCGGAGGA AAAGAAGGAA GAGCCTAAGG AAGAAAGTGATGACGACATG GGATTCAGTC TGTTTGATTA GGAGCTCCTT TCAGTATGAT ATTTGGTTCTTTTTTAGAGA ATTG T32 TAACACAGAG AAAGTAGAAG AAACTACAAA SGP1 monomeric SEQID N° 503 ACAAGGACAA CAACAACATG CCAAGAATGG G-protein like ATCATCATATGGTCTATTTC CTTCATTATG protein ATGATCCTGA TGACGACCCA TCTTCTTCTTTGACCTTGAG ATATGAACCT TCTTCTAAGT CTTGGGAGAT C T320a GATCTCCCTACCGGTGGGCT TGCTAACGTC phosphoglycerate SEQ ID N° 504 GCTGCAACCTTTATGAATCT GCATGACTAC mutase GAGACACCAA GCGATTACGA GCCAAGCTTG ATTGAGGTTGTTGACAACTA GATATCTCAG AGAATTTAGG AGGGTTGAAA TTTTGGCGCA AGTTGGAAAGTGATAATGAC TACATTCTAT ACTCTTTCCA GTCTATTTGA ATAAGACATT TTTTTGAGCTTATATTA T320b AGTGATCTCCATCGTGACCTTGGTTTTGATAAGAAA plexus-like proteinSEQ ID N° 505 GAAGCAGCTGCTCCCTTCCTTCTCCACTCCCAGCATCAAGCACATTCCTTAGCACAATCAACCAGTCAACA ACCACCCCAAAACAACCTGCAAAACTCAGCAAAATTCCACCCAAAAACTCCTAGAAGCGCAGTACTTCAG CTCCAGAAAGTCATGAAAACGCAGTTGTAGCACCGTCCCTTTTAGCACCCTTA T320c TAAGGGTGCTAAAAGGGACGGTGCTACAACTACGTcollagen-like SEQ ID N° 506 TTTCATGACTTTTTGGAGCTGAAGTATTTTGCTTCTTprotein GGAGTTTTTGGATGGAAATTTTGCTGAGTTTTGGAGGTTGTTTTGGGGTGGTTGTTGGCTGGTTGATTGTGC TAAGGAATGTGCTTGATGCTGGGAGTGGAGAAGGAAGGGAGAAGCTGCTTCTTTCTTATCAAAACCAAGG TCCCGATGGAGATC T321 TAAGGAAAATAAATGACATG CATTTAGAAC putative protein SEQ ID N° 507 CAATATTCAAGAACAGTGAG TTTATCATCT At2g11600 [A. CTCAAAACAT AAACAAAATG AACTTGGCTTthaliana] CAAATAATCC TTGAACAAAA TAGGGAGATC T322aGATCTCGAGAGAATTTATGGCTTCACTCCAAGAAA putative protein SEQ ID N° 508CCCTCGTGCTGTAAAGCCACCTGATCATTACATAGA At5g22210 [A. ATACATGCGCTTAthaliana] T323 ACAGCTATGACCATTAGGCCTATTTAGGTGACACT cellulase SEQ ID N°509 ATAGAACAAGTTTGTACAAAAAAGCAGGCTGGTACCGGTCCGGAATTCCCGGGATGAACATGAGAGGGAA ACCAAGGCTACTGGTTAATCTCTCAACCATTTGACTTTGATCACCAATTAAGCTCAGATACAATGCACTCA GCAAATCATTGGGGAGGATCATTAGAAATCGCGAACACCGGCGATTCAACGGCGGAGGAATATGACCGGA GTCGGAATTTGGATTGGGACAGAGCATCAGTAAATCATCATCAAAAACAACAACAGTATAATAACTACGA TCAATATTCTCATCGGCATAATTTAGATGAAACGCACAGAGTTGGTTATTAGGTCCGCCGGAGAAGAAGA GAAGAAATACGTCGATTTAGGATGTATTTGTTTGCAGCAGAAAAGCATTCAAATATACTATTTATGGAATTATTATCGCTTTTCTCGTTATCGCTCTGCCTACGATTATCGCCAAGTCTTTGCCTAAGCATAAAACTCGGCCTTCTCCTCCTGATAATTACACTATTGCCCTTCACAAGGCTCTCCTCTTCTTCAACGCTCAAAAATCTGGAAAA TTGCCAAAAAACAATGAGATTCCATGGAGAGGAGACTCAGGTTTACAAGATGGATCAAAACTCACAGACG TTAAAGGAGGGTTGATTGGAGGGTATTATGATGCTGGAGATAACACAAAATTTCACTTTCCAATGTCATTT GCAATGACAATGTTGAGTTGGAGTGTCATTGAATATGAACACAAGTACAGAGCCATTGATGAGTATGATC ATATCAGAGATCTCATCAAATGGGGCACTGATTACTTGCTTCGTACTTTCAACTCCACTGCCACTAAAATT GACAAAATTTATAGCCAGGTTGGTGGTTCTCTAAACAATTCAAGAACACCAGATGATCACTACTGCTGGC AAAGGCCAGAAGACATGAACTATGAACGCCCTGTTCAAACAGCTAATTCGGGGCCTGATCTTGCCGGTGA AATGGCAGCAGCATTGGCTGCAGCCTCCATAGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXACGTAGGAACTG TGGCCCTCGCTATATCTCCTTGGATATTCTTCGCCGTTTTGCCACTTCCCAGATGAATTATATTTTAGGTGA CAATCCCTTGAAGATGAGCTATGTAGTAGGGTATGGAAACAAATTCCCAAGGCATGTACATCATAGGGGT GCATCAATACCCTCTGGTAAAACAAAGTACTCATGCACTGGAGGTTGGAAATGGAGAGATACCAAAAATC CGAATCCTCACAATATTACAGGAGCTATGGTAGGAGGACCTGATAAGTTTGATAAGTTCAAAGACGCGCG CAAAAATTTCAGCTATACAGAGCCAACACTAGCAGGAAATGCAGGACTAGTTGCTGCACTGGTTTCTTTAA CTAGCAGTGGTGGCTATGGTGTTGACAAAAATGCCATTTTCTCAGCTGTTCCACCCTTATATCCAATGAGT CCACCCCCACCTCCCCCATGGAAACCATAATGTGCAAATTTTGCCTTGAAAACCTGCAGCAGCTTAAATTT TGCCTATATTTGGCTGGCTATATCCATGTACAAAATTTCGAGAATAAAGAGTTGTTGTAACTCTGTTTATCTTATGACTCCGCGGCTTAATAAAATTCTTGCATTAA TTTCTTTTTAAAAAAAAAAAAAAAAAA T324aGATCTATCAA GTTTGCATGG TGGGTGCCCT putative prolyl 4- SEQ ID N° 510GTGATTA hydroxylase alpha subunit T327CTACCGAAGGGTACCTTGCAGAAGAAGGGGAGGA expansin SEQ ID N° 511ATAGATTTACAATCAATGGGCACTCTTACTCCAAC TTGGTTCCCGTGACCAATGTTGGAGGTGCAGGAGATGTAAGATCATTGTACATCAAGGGTTCAAGAACTC AGTGGCAACCAATGTCAAGAAATTGGGGCCAAAATTGGCAGAATAACGCTTACCTCAATGGCCAAAGCTT ATCTTTCAAAGTCACCACAAGTGATGGTCGCACTGTGTTTCTTATAATGCAGCTCCTCATTCCTGGTCCTTT GGCCAGACTTTTACTGGAGGACAGTTCCGTTAT328 CCCTTATTGAGCAAAATCTCGAAGCTTGGGGGTAA eukaryotic SEQ ID N° 512GGTATCTTCAGCCTCTTCGGTTCCGGAAGTGCCACT translation initiationGTGCCAGCCTGTTCCAGCCTTGGAAAAGCTTGCAA factor 3CTCTGAGGTTGCTCCAGCAGGTATCTCAGGTGTACC AGACAATCCAGATTGGTAACCTGTCTAAGATGATCCCATTCATTGACTTTGCTGCTATTGAGAAGATCGCT GTTGATGCTGTTAGACATAATTTTGTTGCCGTTAT330 TAACCCCAAA GTTCAAGCAT CTATTGCTGC BTF3b-like SEQ ID N° 513AAACACATGG GTCGTTAGTG GTTCCCCCCA transcription factor GACAAAGAAATTGCAGGATA TCCTTCCTCA AATTATTCAC CAATTGGGTC CTGATAATTT GGAGAATTTGAAGAAGTTGG CTGAGCAGGT CCAGAAGCAG GGTGCTGCTG CAGGTACAGG TGAGGCTGCAGGTGCGGCCG CAGCACAGGA AG T331 TTCCAAAAGTACAACAGGTGTTAGAAGTGCGTTCG RNApolymerase SEQ ID N° 514 GTTGATTCAATATCCATGAATCTAGAAAACAGGAT beta″TGAGGGCTGGAACAAATGTATAACAAGAATTCTTG GAATTCCTTGGGGATTCTTGATTGGTGCTGAGCTAACTATAGCGCAAAGCCGAATCTCTTTGGTTACTCAGG ACTCATCAAGAGACCCCCCGGGGAATCCCGAGAATTCTTGTTATACATTTGTTCCAACCCTCAATCCTCTTT TT T332b GATCTGGGTG AGGCAAAGAAAATTCTTGGC light harvesting SEQ ID N° 515 ATGGAGATAA TTAGAGATAGACATTCAAAG chlorophyll a/b- AAACTCTGTT TATCTCAGAA AGAATATTTG bindingAAGCGAGTAC TACAACGTTT TGGCATAGAT GACAAGACTA AGCCAGTTAG TACTCCACTTGCTCCCCATG TTA T333b CCAGCCGCAC CCTCACCACC AAAACTCCAT putative outer-SEQ ID N° 516 CGTCGGACCT CCCTTCACTA CGCAATAGCC membrane proteinATAAATGAAA CTTCACCTCA CACATGCCCT AAGCTCTTCT TCTTCATTGA CAGACCCAGA TCT335b GATCTGGAAC ATGACACAGC TGAGGCGTCT disease resistance SEQ ID N° 517GCGTCTACTG AGTAGAAACT ATTTGTGTAA like protein GCCTAAA T336a GATCTGGAAACCCCAAAAGT ATAGAAGCAA ambiguous hit SEQ ID N° 518 TTCTTGAGGT TGAGGATATCATATAAACTA CTGTACCATT GGATTTCTTT CCATAATTCT TGAGGTTGAA TATCTCAGGCAATCTTTGAT TCATATGGGA A T336c GATCTGGGGA ATACTGACTT AGTGACTTAC putativeprotein SEQ ID N° 519 AATGTTATGA TGAACCTATA TGCTAAAATG At5g09450 [A.GGAGACCTTG AGAAACTACA GTCGTTAGTG thaliana] CAAGAGATGG AAGATAAGGG AATTGCT337 TAAGGAGAAA CAGAGAAGGA AACTACTGAG putative protein SEQ ID N° 520AAATGATAAT GCAGAAAACA CACCAATACT At3g52110 [A. thaliana] T339TGGTGTCGGAAGAATACACATGGCACCATTTAGTG putative arginine SEQ ID N° 521ACGAATATTTGTATATGGAAATAGGAAATACGGCG methyltransferaseACCTTTTGGCAGCAACAAAACTACTTTGGGGTTGA CTTGACACCTTTGCACAGATC T340GGTTTCATCACTGGTTTTGACTTTGGAGCTTGATTT proline SEQ ID N° 522AGTGGAGTTTTCATGCATAGAAATTTCTGAATTTCT dehydrogenase likeTCTATTGGAAGCTTGAAGAATAGGAGAAGAGGCGT proteinTCCTTTTCCTTGCCTATGTTTTCTCCTCAATCTCCTCCCCTTTTCATTCTCTGTTTTTCCGTCTTTCCCCAGAT C T341TAATGGAGGGCAAGCTGAGGAGTGGAACTACTCTC thymidylate kinase SEQ ID N° 523ATTGTTGATCGCTATTCTTATTCTGGGGTGGCATTT TCATCTGCCAAGGGACTTGATATTGAATGGTGTAAGGCCCCAGAAATAGGATTGTTAGCTCCAGATC T349TGCCAACAGTTCTATGCACATTGGAGATGTCACAA putative protein SEQ ID N° 524TCCCATATCAAATTGCACAAACAGGGCTCTGGGAT AT4g24350 [A.TGGCTGAAACCAAATGCAACTCTGGAACCAAATGA thaliana]TTTTGCTCAATTTGATTTCAAGAATTATAATGTGCC AAAAGGAGGGGATAACAAGTTGGGGCGTGTTGGTTATAGCACGGAGCAGTTTTACTCAACTTCAGGGGAG GTCAATGTACCTCAGAGACCAGTTTGGTTTA T35GATCTCCGTC CGAGTGAATA ATGCATTTCT putative protein SEQ ID N° 525TTTGGCAGGC AATGAAGAGA ATCGGGTGGA At1g70660 [A. TCAAAAAGGT TTGGTTCTGAAATGTTGTAT thaliana] TTTGTAACTG GAGATTGGAG AAAGAACATT GTAGATGAAAATGTATATAG CCTTATTGCT CAGATAGTAG CAACTGTTGT CTTA T351aTTTTCGACAAGCTTGATGAAGATGGTGATGGATTA putative protein SEQ ID N° 526GTAAGTTTAGGTGAACTCAAAGGCCTTCTTGATAA CG14861GATTGGAGCTTGTACAGATCACTACGCAGTCATAA [Drosophila GATC melanogaster] T352GATCTGTTGA TGCAGATATG TGGCATGGGA serine/threonine SEQ ID N° 527ATCAGGATTT GCTATCCTCA AACAATGTCA kinase-like protein CAATCAGTGT ACTAATAT353b GATCTGTCAT TGATGTTCAT TACTACAATC glucan 1,3-beta- SEQ ID N° 528TTTTCTCTGG CATGTTTA glucosidase T354TGTCACAATTCCATCTCAAGTCGCTCCAACTGGGCT putative protein SEQ ID N° 529ATGGGATTGGCTGAAACCAAATGCATCTCTGGAAC AT4g24350 [A.CAAATGATTTTGCTTAATTTGATTCCAAGAATTATA thaliana]GTGCACCAAAAGGAGGGGATAACAAGTTGGGGCG TGTTGGGTATAGCACTGAACAGTTTTATTCAACTTT356 TAACTGAGGC ACAAATGATT GACCACATGT glycine SEQ ID N° 530 CAAAATTAGCTTCAATGAAT AAGGTTTTCA decarboxylase AGTCATATAT TGGGATGGGA TATTATAACAmulti-enzyme CTTTTGTACC ACCTGTTATT TTGAGGAATA complex P subunitTTATGGAGAA TCCTGCTTGG TATACTCAGT ATACTCCTTA TCAGGCTGAG ATTTCGCAGGGACGTCTCGA GTCCCTGCTA A T357 CCATTCTTCTCATTTCTGATGTATTTGGATATGAAGendo-1,3-1,4-beta- SEQ ID N° 531 CTCCACTTTTGAGGAAGATAGCAGATAAAGCCGCAD-glucanase GCTGCAGGGTACTTGGTGGTTGTTCCTGATTTCTTCTATGGTGAACCTCTTGATCGCGAGAAACATAACGT ACAGACATGGTTA T36 TGACGTGCGTAGAGATCTCC GAGATTATCT putative protein SEQ ID N° 532 AGATAGTTTCCATGGGCTGG GACTTTTCCT AT5g13800 [A. CTTCCCACCA CTATCAGAAA GCTCACAGAAthaliana] CTTGTATGGC AGAAAATTAG TGCTCCCGAG AGCATTGCGG AGGTGCTTA T361TAAGCACCAC AATTTGCAGC TGTTACCAGT photosystem II D2 SEQ ID N° 533CGATCGCGAT CGCGCCTACA TGCGCAGACT protein TCCACATCTG TACCATTGTACCATAGTAAC CTTGTTCTGT CTCTTTGTTC ACTTAGAAAT GCTATAAATA CTGCATACAGATGACTATAC ACATTAGCTG ACGCTTGATC ATTCATTGAG GAACCTTGTG GTTTCCACAATTTTTCACTA AGCAGTCGGC ACATGATGTG TTAGTCAATC CCATATGGCA CTCAAATACTGTGTGCCGTA CATATGGAAT AGGGAACTAA GAGAGTTACA TACGGGAGAT CAATAAGGGCTCAGCAACAG GAGTGTCTTC A T362 ACGATGTGCT CCCGGTCCCG AGTGTCTCGC 14-3-3like protein SEQ ID N° 534 GCAGTGTGTC ATCCTCAAAA CCAGCCTTGG GTAAAAATGACAGGTAGGAT GACAATGTTA TGTTATTGTT GGACTTGTGG GAAGTAGTTT GGTCCTTTGAACTTTGTTGC CGGAAAAGCT ATCTAAAGCA CTTTCTGATT TGGGCTTTCA GGACTTCAGGTCATTTATTC CGCCTTA T364a GATCTGTGGA AAAGGAAAGC TGGAGAAACT NADH SEQ ID N°535 TGCTGTGCTG TAATTTATGT ACAGTGCTAT dehydrogenase-like TTGGCTGCTCAACTAAGATT GTTTTGATTC protein TCTCTTAGTC TTATGTTATC TTTTTTCTTGAAAATCCTTG CTTTTTCTTT CTTCTCTTGG AGTTGGGGGT CAATATCCTT TGTTTGTGGT GT364c GATCTGTGGA ATGCAATTGG TTCGTAATAT B12D-like protein SEQ ID N° 536CTGCGGCAAC CCTGAAGTCA GGGTGACCAA GGAAAACAGG GCAGCAGGGG TACTGGACAATTTTTCAGAA GGGGAGAAAT ATGCTGAGCA TGCTCTTAGG AAGTTTGTCC CCTTCTGTAAAGTTAGCATT TTCTTCTGCT TCCCCGTTTT T365a GATCTGTCGA ACCAGAGTTG GAAATGGAGGputative protein SEQ ID N° 537 AAGAGGATGA TCCTTATCCT CCATCCACTGAt4g11570 [A. TGGCCGTTGA TGATGGTTTC TGGTAACATC thaliana] TCTGCAATGTACAGTAGTTG TGCTTACTCA GGACTGATCG TCTAAGGACT TTTATGAGAC ATTCTCGTGTGTTACAATAC AAATATGACA TCTTTGCCTT A T366c GATCTGTACA AGCAAGACTGGATTGGGAGA phospholipase D SEQ ID N° 538 GGAGGACTAT GCGAATGATGTACACTGACA TAATTCAAGC TCTAAAAGTA AAGGGAATTG T T367 GATCTGTTCT TCAATATAACAGAACGTCTT putative protein SEQ ID N° 539 TTTTCCTTA ORF 1901 [Nicotianatabacum plastid] T368 TAATGCTCTC TCTGCACATA CTGGTACATA putativeglyoxalase SEQ ID N° 540 AATAATAATA TTACAAAAAA GGATTTTTAC GGTATGTTTGGGTTGTTGGA AAAGGGGTCT AAATTTATGA GGGGTAAAAT CACTCTTTTT GCCGACAATATCACTCAAAA ACAAATATCT ATCATGTCCA AAGCTAAATT TTCCATCATC AGAGATTCCACTTCTCGTGA GCAGTTCATA TTTGCACCTC TGCTTCCATT TTCGTGAATG AAATTAGGCA TTGTT369 GTAATATCTGCGGCAACCCTGAAGTCAGGGTGACC B12D protein SEQ ID N° 541AAGGAAAACAGGGCAGCAGGGGTACTGGACAATT TTTCAGAACGGGAGAAATATGCTGAGCATGCTCTTAGGAAGTTTGTCCGAAACAAGTCTCCGGAGATTAT GCCATCTATCAACGGCTTCTTTAGCGATCCAAAGTGAAGTTTGACATGGATTA T37 GATCTCCAAG CCTAGCTCCA GCACCAGCAC fasciclin-likeSEQ ID N° 542 CAGGTCCCGA ATACACAAAC CTAACCGACT arabinogalactan-TACTCTCCGT TGCTGGCCCT TTCCACACAT protein 7 TCCTTA T370a GAAAAAGGGAGAAAAAGACT ACACTTAGGA putative ankyrin SEQ ID N° 543 GCACGTTATTCGCCTATTTG AAGCTAAAAA protein CCTACCCCCA CATCTGAAAA GATCGGGAATCGAGGATATA TACAGATC T370b GATCTGTCAA AGGCCAAGTA TTTCACAGAT putativeacetone- SEQ ID N° 544 GAAGGGTTTG GATCAGTGAA GAGAGTTTAC cyanohydrinlyase ATTGTGTGCA CAGAGGATAA ATGGATACCA GAAGAATTCC AACGATGGCA AATTGACAACA T372b TAATGCACCA CTAAACAAGC ATGATAGGAG putative 12- SEQ ID N° 545TACTTTCTAT ATGACAGATC oxophytodienoate reductase 2 T372c GATCTGGAAAGGTGGGTGTA TTATCAGGGC 6-phosphogluconate SEQ ID N° 546 AGTGTTCTTGGATCGGATTA dehydrogenase T39G GATCTCCAAC TGAAATGAAA TGAAGAGGAA maturaseSEQ ID N° 547 GACGATGAGT CCTGAGTAAT GTCAGGGGAG GAGGACTTGG GATCGCGTAAAACACAGACA TCGCCATTGC AGACGAATTC GCCAGAGTCT GAGGACTCAG GTGAGAAGCAGCTACAGAAG TTGAACAAAG CCATAGTAGG AATTGAACCT AAGTAAATTA TATATCCCGATCAAAGAGCT GACGAAAGGA ATGAGCAGAA CGTGGAGTGT AGTGGATATT ATTCGACTAACGAAGACTCT TGGAATAGTT AGAGTAAAAA GTTCCCAAGA GAGCGTCTTT ATGGCGCGCGTCAATCACAT ACAACAAGGA TCAAGGGAGA TCACTACGCA GTCAA T401 TAACACATACACACGCATAA CTCACGAAGT iron(III) ABC SEQ ID N° 548 GGCACGTGTA AAAAAGAATTCCATCGAAGT transporter-like GTTCGAAATT CAAAGGACAC AAAAATCTCT proteinCTCTAAAAAT TCTTGAAAGA GCTGGTGGAT GAAACAGATT CTCTTACAAA CACTTTCAATTCAGACGTAC GATAATTAGC GTGAAGACTT GAAAAGTAGC CACTGCAAAG GAAATGATCCCATTACTGTT AACAAAGGCA TATTC T402 AGAAAAAGTCCGATCACCGGGCGAGGAGTCCGACphenylalanine SEQ ID N° 549 AAAGAGTCCACACGCAATGTGCAATGGACAAATCA ammonialyase TTGATCCAATGTTGGAGAGTCTCAAGAGCTGGAATGGTGCTCCTCTTCCTATCTGTTAGTTGTTTTGCTTGAT TTCGCGCGGCGGGAACTTTTGTTA T404GATATTCTTGGTGGAGTTTTAGCTGCGTTATGATAC fatty acid 9- SEQ ID N° 550TTTTGAAATTGAATTTGGAAAGCTCCTGCTTGGTTC hydroperoxide lyaseTAAGGTGACTTTCAAGTCAGTAACCAAGGCAACGT CTTA T405a GATCTTAGGG CAGGGCATGAACAAAGTCTA lipoic acid synthase- SEQ ID N° 551 TCTGTGCTTA like proteinT405b GATCTTAGAG TGTCTAGGGT TGGGCCAGGA putative protein SEQ ID N° 552GGGTCTCTTA tRNA-Ile [Spinacia oleracea] T406GGACCTGATACGGATACGACAGCCTTTTGGGAGTC putative protein SEQ ID N° 553GGCGCAACATAGGCCCTTTGTTCTCCAAAACTATAC At2g36290 [A.TCTGGGGCTTGTTTAGTATTGGATTCAATGACTCTT thaliana]TGTTATTGTACAAATTTGAATATTTGTCAATATTATCAAATGATTGTTTAGTTGCTTTATTCAAGTAATGAA TGGTTATGTGTTA T407GTTTGAAGATGAAACGTTTGATTTGGAATTTTCTCC putative protein SEQ ID N° 554TGTTTTTGACCCCGCGCTTTATCCGGAGAAATATGT At1g24480 [A.GTCGGAGATCGAACGGACGTTGAAGGCCGGAGGG thaliana]TTTGTGTTTTGCACGTGGCGTTATCTAGACGGGCTG ATAAGTATTCGGCGAACGATTTGTACAGTGTTGAGCCGTTGAAGAAACTGTTTA T408 GATCTTGAGT TCAATTCCAA AGCCATTTAC uraciltransporter- SEQ ID N° 555 CATTATTTAC AACAATGCTT GGTTCTTTAG like proteinCTTGTTTTTA GCAGGGGGAC TTTATTGTAT TCTTTCATAT TTGAAGGGGA AAAAGAAAAATCAAAAGCAC GTAAATCCTT TGCTGCCTAA TGCATCTTAG TGATGTCTCT T409aGCGCGAAACGCGCTATCTGTCGGGGTTCCCCCGAC hemolysin SEQ ID N° 556CCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAGGTTC TCATTTGAATATTTGCTACTACCACCTAGATCT409b GATCTTGGCC TGTTGACAGA TTTAGCCGTT putative protein SEQ ID N° 557TTTCATATAA ACTCCAATAG ATTTTCAGGC At1g49490 [A. ACTATCCCAA AATCCTTTTCTAAGCTCCAA thaliana] CTTCTCTATG AACTTGACGT GAGTAACAAT CTTTTGTGTGGTGAATTTCC TTCGG T409c GATCTTGGAC CCAGAAATAT GCCATGGGAT ubiquitin SEQ IDN° 558 GAAAACATTT GGCTTTACTC CCATGAACAT conjugating enzyme CGGGCCTTTATGCTATAGTA GTAAATAAAA ATAGGCGCGG AGCACAATTT TCTGATATTG GTGTCTTTTGTTATCTGACG TTGTGTC T410 GAGAGAGCTAGAGCGTGGCGTGAAATGTATTTCTT berberinebridge SEQ ID N° 559 GCATAACTATGATAGGTTGGTTC enzyme T411c TCTTGACCAAGATTGACAGG CGT elongation factor-1 SEQ ID N° 560 alpha T414GATCTTGAAGACTTCTGTGCTTTCCTTTAGTGGCTT hexameric SEQ ID N° 561TTGTTGTGCTCTGTGTTTA polyubiquitin T418TCTTCCTCTGTTGATGCTGTGGAGAGAGCTAGAGCG berberine bridge SEQ ID N° 562TGGGGTGAAAAGTATTTCTTGCATAACTATGATAG enzyme G T419TGCAGCGATTGCTGGGTTTGAGGTAACTGTCTTGG collagen-like SEQ ID N° 563CTTAGTAATGCAATTAGTAGTGTCAGACCCTTGTAC proteinTAGCTCCGGAACATGAATCTTATATGTATTTATTCAAAGAACATTGCGACAAATCTTTGTTATGAATTGTCTTTCTGTGCGTTGTATGTTTCCTTTGGGTGTATTTCGTACGAAGGAAATATTTTCCACGAAAAATATTTCCTA GAAAATAAATGGTTTGCTTA T420aGATCTTGCAC TGTAAACACA GTACTTTGGA putative protein SEQ ID N° 564ATACAATTCA ACTTCTGTTT CCTAAAGAAA At3g27330 [A. TAGAAGCAAG AAAAGCAGCTGGAGCTTTGA thaliana] ATAGTAGAGA AGCTCGACGC AAAAGTCCAG TAAGAGCTGCTACAGCTCAT TCTAACATCT CTAGCAGCAG AAT T420b GATCTTGGCT GCAAGTGGGTCATTCTTGGT putative SEQ ID N° 565 CATTCGGAGA GGAGACATGT AATTGGAGAAtriosephosphate AATGATGAAT TTATCGGCAA GAGGGCTGGG isomerase TATGCTTTGAGGCAAGGTGT TGGTGTTATA GCCTGTATTG GAGAGC T421TGTGTTAGGCTTGGCAAAGCCGAAACCCTTCCCAC high-affinity nitrate SEQ ID N° 566AGCCATTGTGGCCATCCTCTTGTTCTCCCTTGGAGC transporterTCAAGCTGCATGTGGCGCTACCTATGGTGTCATCCC TTTCGTGTCGCGAAGATGACTAGGCTTA T422cGATCTTGCCA TGGACGTAAT TATCAACAGC wound-induced SEQ ID N° 567 AGCCATATTGGGTCCTG WRKY-type transcription factor T423TGACTGCGTAGTGATCTTGATGGTGAATTGACCTTG annexin SEQ ID N° 568AAACAAGTTGTTCAATGCCTTTGTTCACCTCAAGCC TACTTCAGCAACATATTGATCGCGTCCTTAT424a GATCTTGAAT ACTATTCGAA ATTCAGAAGA H+-transporting SEQ ID N° 569ACTGCGTGGA GGGGCTATTG AACAACTCGA ATP synthase I AAAAGCTCGT TCTCGCTTACGGAAAGTAGA AAGCGAAGCC GAGCAGTTTC GAGTGAATGG ATACTCTGAA ATAGAACGAGAAAAATTGAA TTTGATTA T424b ACAGCTATGA CCATTAGGCC TATTTAGGTG auxin-inducedSEQ ID N° 570 ACACTATAGA ACAAGTTTGT ACAAAAAAGC protein AGGCTGGTACCGGTCCGGAA TTCCCGGGAT GAAATCACAA CAATGGCCAA AGAGGGAACA AAAGTGCCAAGAATCAAATT GGGTTCACAG GGGCTAGAAG TGTCAGCTCA AGGACTTGGT TGTATGGGTATGTCCGCTTT TTATGGGCCG CCCAAACCCG AGCCCGATAT GATCCAACTC ATTCACCATTCCATCAACTC TGGTGTCACC TTTCTTGATA CATCAGATGT GTATGGGCCC CACACCAATGAAATCCTACT TGGCAAGGCG TTGAAGGGAG GGGTGAGAGA ACGAGTTGAG TTAGCAACAAAATTTGGAGC TATTTTTGCA GATGGAAAGA TAAAAGTGTG TGGAGAGCCA GCCTATGTAAGGGCAGCATG CGAGGCTAGC TTAAAGCGAC TTGATGTTGA CTGCATTGAC TTGTACTACCAGCACCGAAT TGATACACGC GTGCCTATTG AAGTCACGGT TGGAGAACTT AAGAAGCTGGTTGAAGAGGG TAAAATAAAA TATATAGGTC TATCCGAGGC ATCAGCATCG ACGATTAGAAGAGCACATGC AGTTCATCCA ATAACAACAG TACAATTAGA ATGGTCTCTA TGGTCTAGAGATGTAGAGGA AGAAATAATC CCTACTTGCA GAGAACTCGG TATTGGGATT GTGGCATACAGTCCACTAGG ACGGGGATTT TTGTCATCCG GTCCAGAGCT GCTTGAAGAT TTGTCAAGTGAAGATTTCCC AAAGCATCTC CCAAGGTTCC AGGCTGATAA TCTTGAGCAT AACAAAATATTATATGAAAG AATTTGTCAA ATGGCGGCAA AGAAGGGATG TACGCCATCT CAACTAGCCTTGGCTTGGGT ACATCACCAA GGAAATGATG TGTGCCCCAT CCCAGGTACC ACAAAGATCGAAAACCTCAA CCAAAACATT GGAGCTTTGT CAATTAAGTT AACAACAGAA GACATGGTGGAACTTGAATA CATTGCTTCA GCTGATGCAG TCAAAGGTGA AAGAGATGCT TCTGGTGCAAATCACAAAAA CTCTGATACT CCACCATTGT CAACTTGGAA GGCTACGAGA TAAGATTTTCGCGCACTTTC CACGTTACAA TGTATCTGAA ACATGTTCTT GTTGGAAATA GTAAATATTATAAAAGTTTA AACAAGTGTC TAGGCTCATT TGTACTGTCG AGTCATCCCA GAATATTCACTAATCATTGT TCATATAACT TG T426b AGTGATCCTC AAGCATTAAT TTGCCACTTT hemeoxygenase SEQ ID N° 571 TACAACACAT ACTTTGCGCA TTCAGCTGGA GGTCGCATGATAGGAAGAAA GGTGGCTGAA AAAATACTCA ACAAGAAAGA GCTGGAATTC TGACTGCGTAGTGATCTTGG AGTGAATATG GACGACGACT ACTTACTGCG AAATGCTAGT AGTCGGTAATTCTTCTTCCT CTGTTGATGC TGTGGAGAGA GCTAGAGCGT GGGG T426c GATCCGGGTCACTTCCCTAC ATTGGGTGGC probable SEQ ID N° 572 AAGTGATGCT TTATTAGTGCTTTTCTCCCA transcription factor CGTCCAAGAG GCAAATTGAC TGAAAAATAA T429cGATCTTCTAACAGTAAATGAAATATGTTGCGACAC helicase-like protein SEQ ID N° 573ATTTAGAGAATCTGCAGAAAAAAGAGGGTTGTTAC ATTGTGATAACAACTTTGATTGAATGTATGTTAGAGGCTGCATGTTATCAAATGCCTTATAGTTTA T430 CCTACATTGGTCCTCGCCATAACGTATTGGATGACAputative ABC SEQ ID N° 574 GGGCTAAAGCCCCAACTCTAGCCATTCCTTTTGACAtransporter CTGCCCGGCCTGCTGAGCTATGTGATTGTTTCACAAGGCCTCCGGTTAGCCCTTGGCGCCTTGATCATGGAT GCTAAACAAGCTTCAACTGTGGTCACTGTCACCATGCTAGCATTCGTTCTAACAGGAAGGTTCTACGTGC ATAAAGTGCCAGCTTGTGTAGCTTGGATTA T431GACTGGAATGGCTGATCGTAAGATCGCAATGCCAG beta-glucosidase SEQ ID N° 575ATGCCATCCCGGATCGTCAGAGAGTGAACTTTTATC like proteinGTGGGCACCTTTCGGCAGTTCAAGAAGCCATAGAG CTCGGTGTGAAGATTA T432a GATCTGCAAACAATGACTGG AAATCTCTTA phospholipase D- SEQ ID N° 576 CTCAGGTAAAGGAGGTAGGA ATATATCTCG like protein CTGGTTGCTC AGATATAGCA AAAAAGGTTGAAATCTACTA TGACAACCTT TGGAAACTTG CCCACCTTGA TGTTCCAGCT TACACAAGATCAGTTTGGGA TTCACAGTGG CAGATTA T434 AATACGACTC ACTATAGGGC GAATTGGGCCputative SGP1 SEQ ID N° 577 CGACGTCGCA TGCTCCCGGC CGCCATGACG monomericG- GCCGCGGGAA TTCGATTCTG ATCTCGGCGG protein CGAATTTGCC CCAACTGCAGCAGCAGCTGC TATCTCTTCC TCTATCTTGT GTTTGTGTGC ATGCTGTGGA TCAGTACCCCGTCTACGCAA CTGCAGCAGC AGCTGCTATC TCGTCCTNTT GCTGACTGCG TAGTGATCTTCAAGTTCATT ACAGCAAAGC TCTTCAATTT GCCATGGACA TTGGAGCGTA ACCTTACCATTGGAGAACCA ATTATTATTT TTAGGTTT T436a GATCTTCACA GTAGCATCAG GTCATACTGAsubtilisin-like SEQ ID N° 578 CAGGTGGTTT TCCGGGACTC TGACACTGGGproteinase AAGTGGTCTA AAGATTA T438c GATCTTCAAA TTTCTTTGAT TCTAAAGTAA N-SEQ ID N° 579 TGAAAGAAGC ATTA hydroxycinnamoyl/ benzoyltransferase T439GATCTTTACG GGCCCTATTT ATTCTTCAAA acyl CoA reductase SEQ ID N° 580GGAATATTTG ATGACATGAA CACAGAAAAA TTACGTAGAG CAGCGAAGGA GGCTGGTATTGAAATAGACG TGTTCAATTT TGATCCCAAG AGCATCAACT GGGAGGATTA TTTTATGGACACTCACGTAC CTGGCGTTGT AAAATATGTA TTTA T440 GATCTTGGAG TGAATATGGACGACGACTAC berberine bridge SEQ ID N° 581 TTACTGCGAA ATGCTAGTAGTCGTAATTCT enzyme TCTTCCTCTG TTGATGCTGT GGAGAGAGCT AGAGCGTGGG GTGAAATGTATTTCCTGCAT AACTATGATA GGTTGGTTA T441a GATCTATACC AGAAGGAGCT GTTGTATGTA60S ribosomal SEQ ID N° 582 ATGTGGAGCA TAAAGTGGGA GATCGTGGTG protein L2TTTTTGGTAG ATGCTCTGGT GATTATGCCA TTGTGATCAG CCACAACCCT GATAATGGTACCACTAGGGT TA T442 AAAACACCAATTGTCTGTAAACCTTCAGAAATCGC ripening-relatedSEQ ID N° 583 CATTGAACGCGCTTTA hydrolase-like protein T443CCTAAATCTATCAATATGGATGAAAGTTTGGGGGT cytochrome P450 SEQ ID N° 584TACAGCGAGAAAACGCCACTCTTTGAAAGTAATAC hydroxylaseCAAAAAAGGCTTGAGAACTTACGTATTTGAGTTTTCATAGTTATGTTTTGTGCATATTTTCTTACTTATATTTGGAGTAAACCAGTATTCCTGTTGTGTTATGAACAA GTTGTAGTGCTGCCTACTGGAGTTTGTGTTAT446a GATCTTTACA AGGCAGCCGG GGGATTCAAG receptor like protein SEQ ID N°585 GTCAGTGAAC TAATTGGAGT TGGAGGCTTT kinase GGTGCTGTTT ATAAGGGTATTTTGCCTACT AATGGAGCTG AGGTTGCGGT GAAGAGGATA GCAAGCAATT CTCTTCAAGGAATGAGAGAA TTTGCAGCGG AGATTGAAAG CTTAGGCAGG TTA T446bCGACTGGGTAGGGATCTTTGAAGCCGCTAGCAATC lipoxygenase A SEQ ID N° 586GAACTAAGTTTGCCACATCCAGATGGTGACCAATT TGGTGGCATTAGGAAAGTGTATACCCCAGCTGATCAAGGTGCCGAGGGCTCCATCTGGGAACTGGCTAAA GCTTATGTTGCAGGGAATGACTCAGGTGTTCATCAACTAATTAGTCATTGGTTA T447 GATCTTTGCAAGGATTTCTGCAAAAGAGAAAGAAT putativeprotein SEQ ID N° 587 AGAATTCAAGCAACTTCCCCATATCATCACTAGCT At2g34600 [A.CTAACAATTATATTACTAATAATATGTGATGATCTT thaliana]CTATTTCTTTTTACTTTCATTATTTTACTTCTCCTAG TGTGGCTA T448GATCTTGGAGTGAATATGGACGACGACTACTTACT berberine bridge SEQ ID N° 588GCGAACTGCTAGTAGTCGTAATTCTTCTTCCTCTGT enzymeTGATGCTGTGGAGAGAGCTAGAGCGTGGGGTGAAA TGTATTTCTTGCATAACTATGATAGGTTGGTTAT449 GATCTTTTCT GGCCAACTCG GGAACCTACA putative integral SEQ ID N° 589GCTTGCAGCA GCCTCTCTTG GCAATCAAGG membrane protein CATCCAATTA TTTGCTTATGGCCTTATGCT AGGAATGGGC AGTGCAGTGG AAACGCTTTG TGGCCAAGCA TATGGAGCTCACAGATATGA AATGCTAGGA GTCTACCTGC AAAGAGCAAC AGTAGTACTT TCCTTA T454GATCTGTGGA ATGCAATTGG TTCGTAATAT B12D protein SEQ ID N° 590 CTGCGGCAACCCTGAAGTCA GGGTGACCAA GGAAAACAGG GCAGCAGGGG TACTGGACAA TTTTTCAGAAGGGGAGAAAT ATGCTGAGCA TGCTCTTAGG AAGTTTGTCC GAAACAAGTC TCCGGAGATTATGCCATCTA TCAACGGGTT CTTTAGCGAT CCAAAGTGAA GTTTGACATG GATTA T455AGTAATCCCA AAGTTTATCA ATCTAGCCAT putative dTDP-D- SEQ ID N° 591GAGGGGGAAG CCTCTTCCTA TTCACGGAGA glucose 4,6- TGGTTCAAAT GTTAGAAGTTATTTGTACTG dehydratase TGAGGATGTT GATGCGGCTT TCGAGGTTGT TCTTCACCGAGGAGAGGTTG GTCATGTTTA TAACATTGGA ACTAAGAAAG AGAGCAGGGT GATTGATGTTGCCAAAGAGC ACTACGCAGT CG T461 AAGATTGCGA GAAGTCAAAG AACTGAGGTC putative6- SEQ ID N° 592 TTTTGATGTT TTCTTTTTAT TTGACCTAAT phosphogluconateTGCCTAAGGT TCTTCCCGTC ATTGAATCTG dehydrogenase GGAGGCTAGA TTCTGTAGTATCTGTCATGT GGTCGCTCAA ATGTTGGAAC TTTACCTATA TTGTTGTGAA GCCTATTTGTATCTTTA T463a GATCTTAAGT TATAAGTACG TTTCTTTTAT chaperone GrpE SEQ ID N°593 TATTTTCTAT AT type 2 T463b GATCTCACCG GGAAAGTGCA CCAGCTGCCA putativeprotein SEQ ID N° 594 TGCTGTATCA AGTTCA At2g39440 [A. thaliana] T464TAGCGGATAA CAATTTCACA CAGGAAACAG epimerase/dehy SEQ ID N° 595 CTATGACCATTAGGCCTATT TAGGTGACAC dratase-like TATAGAACAA GTTTGTACAA AAAAGCAGGCprotein TGGTACCGGT CCGGAATTCC CGGGATCTCT TTCTAATCTC TCCGCTGCCTCACTTTTCTC CTCCAAATTT TTAGAGAATG GGAAGCTCAG GTGGCATGGA CTATGGTGCTTACACCTATG AGAATCTTGA GAGGGAACCT TACTGGCCAA CCGAGAAGCT CCGTATTTCCATTACTGGGG CCGGAGGATT TATTGCTTCC CACATTGCTC GTCGTTTGAA GAGCGAGGGCCACTACATAA TTGCCTCCGA TTGGAAGAAG AATGAGCACA TGACAGAAGA TATGTTCTGTCATGAGTTTC ATCTTGTGGA TCTTAGGGTT ATGGATAATT GCTTGAAGGT TACAAAAGATGTTGATCATG TCTTCAACCT TGCTGCTGAT ATGGGTGGCA TGGGCTTCAT TCAGTCTAACCATTCTGTTA TTTTCTATAA CAACACTATG ATCAGTTTCA ACATGATGGA AGCTGCTCGGATTAATGGTG TCAAAAGGTT CTTCTATGCA TCTAGCGCTT GCATTTACCC CGAGTTCAAACAACTTGAAA CAAATGTCAG TTTGAAAGAA TCTGATGCAT GGCCAGCAGA GCCTCAAGATGCTTACGGCT TGGAGAAGCT TGCGACCGAA GAGTTGTGCA AGCATTACAA CAAAGATTTTGGAATTGAAT GTTGTATTGG AAGGTTCCAT AACATCTATG GTCCATTTGG AACTTGGAAAGGTGGAAGGG AAAAAGCTCC TGCCGCGTTT TGTAGAAAAG CCCAAACTGC AGTAGATAAGTTTGAAATGT GGGGAGATGG ACTTCAACCA CGTTCATTCA CCTTCATTGA TGAGTGTGTTGAAGGGGTTC TCAGATTGAC AGAGTCTGAC TTCCGGGAGC CAGTGAATAT TGGAAGTGATGAGATGGTGA GCATGAATGA CATGGCTGAG ATGGTTATTA GCTTTGAGGA CAAGAAGCTTCCTGTCCACC ACATTCCTGG CCCAGAAGGT GTTAGTGGTC GCAACTCAGA CAACACCCTTATAAAAGAGA AGCTTGGTTG GGCTCCGACA ATGAGATTGA AGGATGGTTT GAGAATTACATACTTCTGGA TCAAGGAGCA GATCGAGAAA GAGAGATCTC AAGGAGTTAA TATTGCAAATTATGGATCGT ATAAGGTGGT GGGCACTCAA GCTCCAGTTG AACTCGGTTC CCTTCGTGCTGCTGATGGCA AGGAATAAGT TCATCCCTTC TATTAATTGG AAGCCAAATC ACTGCTATGACATTGCTGCT TTATTAATAT GGTTGTCGTA GGTGAATGTG TTAAATTTTC AGTAATTGTTGGCTTTTCTT GGTTTTGAAT CTTGTAATTT AAGCCCCTTG GCTTGTGGGG GGGATGGTTGGATGCTTCAG CTGTATTTAT CAGTTGTTTG AGAAGATCTA TATATGATAA TCCAATAATTGGCAAAC T465 TAATGGCAAA GGGATACAAC CAAAAGAAGG putative SEQ ID N° 596GTATTGATTA TCAAGGAACA TTCTCATCCG retroelement pol TGGTGAAGAT GGTTACTGTAAGAGGACTAC polyprotein GCAGTCA T8 TAAACGTGGT GGATGTTTCT TATGGAGGAGputative polypeptide SEQ ID N° 597 AGAATGGTTT CAATCAAGCA ATTGAGTTGTchain release factor CTGCTGAGAT C T9 GCATCAGGAA CACACAAGAG AATACTGTATputative adenosine SEQ ID N° 598 TACACAGGGT GCTGATCCAG TTGTTGTTGA kinaseTGAGGATGGG AAGGTGAAAT TGTTCCCAGT TATTCCTTTG CCAAAAGAGA AACTTGTTGACACCAACGGT GCTGGTGATG CATTTGTGGG AGGATTCCTT GCACAGTTAG TCCAAGGAAAACCTATTGCA GATTGTGTCA AAGCAGGGTG TTATGCATCG AATGTCATCA TCCAAAGGTCTGGTTGAACA TACCTTAAGA AGCCCGATTT TGAATCACGG ATATTTCCAT C168 TGCAAAATGTTTGCACCTGA AAGAACACAT putative protein SEQ ID N° 599 TGTCCTTGAT GGATCAt3g52140 [A. thaliana] C187b TAATCACAAA GGGTTGCTCA TCATAACTAA putativeprotein SEQ ID N° 600 TAGCTATGCA GTGATTGAGA CAAAGAATGA P0469E09 [OryzaTGGATC sativa] C20 TAATCCAAGT CCCTAGCATA AACACCAAAC putative glutathioneSEQ ID N° 601 CCCAAAAATA ATTCACAATT CTACAGATAA S-transferase AAAAAAGGACATGACCAATT TATTTATCTA TTACTAATCA ACAATTCTGT AGAACTCCAT GACATACTTATACAGCGCGT GCTTCATTCG CAGTTTCACC AATGGTTGAC AAACCGTTTT GAAATTCTAGGCCATAAAAC GGTTTTTGTA GTATTCAACC AGTCTCTTGG GATC C307 TAAAGCAAAGACGTGGTTAC TACAATCTAC metapyrocatechase- SEQ ID N° 602 TTATGCATCATAGAACTAAT GCATTCTCAA like protein AAGTGTATGG GGTCCTCGGA TC C427bTAAACAATAT TTGTAACATA AAAGTTTCAT putative protein SEQ ID N° 603CTGCTAAAAT TGTGTGGAAG TGAGTACAGT At5g12080 [A. TTCTATTTGG AGGATCACthaliana] T108 TAAACAGTGA TGATGATGAT GTGGGCATCT putative protein SEQ IDN° 604 CTGATGAAGA TGAAGAATAT TTCAGAAAGC P0698A04 [Oryza CTCAGGGCAAGCAAAAGAAT AGGGGTGGGC sativa] ATAGTGTAAA ATCTACCAGA GAAATTAGGTTTCTTGCTAC ATGTGCTCGA CGAAAAAGGG GTAGAACATC ATATGAAGAG GAAGAATCATCAGAACATGA TTTCTGAAAA T114a TAAAGTTAGA TGGAAACGAA CCTTTGCTTG guaninenucleotide- SEQ ID N° 605 TAGCATTATG CATGGTAATA TTATATTGTT exchange-likeCACTACACGT TCCTGATGTA GACCCACCTT protein CAAAAACGGG AGAGCGATCATGTAGATCAA TACGCAGTC T147b TAACAGCATG TTCATTTTCA ATAACTCCTG putativeAthila SEQ ID N° 606 TAATGCCTAT TCAACAAATG AAGTTTGAGC retroelementprotein ATCAGTTGTT TCAGTGGATG CAGATGCATC TTTAGCTTCC GATGTGCCAGTTGATGATTT TCCTGCACCA CCCGTAATAA ATTTGGTAAT CAAATCTTCT AGATC T42TAATCATAAA GTTTTGAGGA AGCACCTCAA putative glucan 1,3- SEQ ID N° 607AAGATCAACT TGTAACAGCA TTGTGGAGAT C beta-glucosidase T207a TAACGATGTCAAAAAATTTC TGTCGGAGAC phosphatidylinositol- SEQ ID N° 608 AGAATCAGAGATCATAATCC TCGAGATC specific phospholipase-like protein T325b TAAACGAGCAAAAGAATAAT AAGGGACTTA urdine SEQ ID N° 609 GCATACTGGT AGCAAGAACC CCAGATCphosphorylase like protein T365b TAAGCCGTAC ATCAAATTGG TATATATTGGtranscription factor SEQ ID N° 610 TCACTCACAA AAGACTTTCT GTACCCTAAC rush1 alfa like CTTGCCAATG GAAGTGGGCA ATGGTAAATT protein CGTGCGAGAATCAAATTTCG ACAGATC MC311b GATCCGGAAC GAAGGCGATG AACCTGACTC putative bZIPSEQ ID N° 611 GATCGGAATA TAATATCACC GCAAATGACC transcriptionalTCGACTCTCA AATGGCGACC TTGACCGCGA activator AACTACAATG ATTCAAACTCGAAAAATGCT CAATGATGTT CAACCTGCTT TATTA

TABLE 2 Sequences with no homology Seq Anno- code SEQUENCE tation SEQ IDN° C103 GATCCAAGATAGCCCTATAGGCGTCCGCATTCCCTGGCATCTC No hit SEQ ID N° 612CCTCCATCCTTTCATCCTGTTTCATTTATGTAATTCAGAAACAGGGTTGTATTTATTTTTGGACCTTGTTTGTAGTATTCCTAGACCGTTTGTGAAGTTGTGACACCAGTTTTGGGTAGTATTTGTTTA C110aGATCCAAGTTGTAAACATTGTGGAAATGGAACATGTAATATAT No hit SEQ ID N° 613ATAATGCTTA C115 GATCCAAAGGTACGGCTTAGCAAAATTACAGACATGATCTCGC No hit SEQID N° 614 TTCACACATTTCCAGAGGCAACAGTAGAAGAAAGATACCATATTGGAGAGCTAAAGGTTTTATAAAAAGTTGAAGAAGGTTTATATTAGCCTCATCTACAATCCTGTTGCAGAGATCAACTAAGTGACT TGAAATGCTTTTGTAGACCATTAC117b GATCCAACACGCAACTGTGAGTATTTTTGAAGAGCTCGAACAA No hit SEQ ID N° 615TATAGAAATTAGAAGTT CACTTTATAT TTGATTA C118aGATCCAAGGAGGGTGGTGTAGCGCCTTCACGTCAAAAGACTA No hit SEQ ID N° 616ATGAAGTTGTCATTA C118b GATCCAACAGCTCAACAATGAAAGAAACAAAGCAAAAGATAA No hitSEQ ID N° 617 TCTTTTTTTCATTA C154GATCCACGGCTATAGGTGATGACGATGGCATTGAGATACCTTC No hit SEQ ID N° 618AGGTTTATTCGAAGGTAGAATCAGTCAAACGCA C124aGATCCACCACAACCCACATTTGATTTGATAGCTCAATCTAAAT No hit SEQ ID N° 619TGGAAGCAATAGAGGTAATATTTAGGGAGCACCAGTTA C155GATCCACCAAAACCCTTGGCAACTTCGTTACTCAGGACTCATC No hit SEQ ID N° 620ACACCAATCCATCCCGAACTTGGTGGTTA C129aGATCCACCATTTGGGAATTTGCTGCAATGTAGGGAAAAGAAAC No hit SEQ ID N° 621AAAAATTGAAATGTCACACACTGACTGAGGTAATTACAAAATTATCATTGATCTTTACATTCAAAGTGGCTTA C156aCGACTGCGTAGTGCTCCACTTACCATAGTTTGAGCACGATAGA No hit SEQ ID N° 622CATTCCGGGATCATCTAGTAAGGATCGCTCATTCAGGAGTTGC TTA C156bGATCCACAAGACATGTTCACCACCAACCGGGTACATGTACCAC No hit SEQ ID N° 623GATGTTTTGACAAAATGTTGTGATTTTTTTGCTTA C156cTTGGGCAGTAATACGCTAATCAGAGATTAAGCAGCATAAACAT No hit SEQ ID N° 624ATGAGGCTGATATAGTTATTGTCGCCCACTTAGGGGAAGTTTA C158 GATCCACAAA ATCAAACGGACTATAACAAA TCCAAAACCC No hit SEQ ID N° 625 TAAGTTTTGAATCTGAAATTCGGGTATAAA AACCCTAGGG ATAGCAAGAA ACGGGGA C138aGATCCAGACAAAACACCTTTGTTATGCTCAGGGTTGAGTAGTT No hit SEQ ID N° 626 TAC138b GATCCAGGTACCTCAGAGCGAGCTGGGCATTAGGTGACTGTTT No hit SEQ ID N° 627 AC146 TGACTGCGTAGTGCTCCAGGGTACAGACGTACAGTCCTTATTC No hit SEQ ID N° 628ATTCTTCACTTA C147a GATCCAGCACATGCAGAACAACTCATCCCATTA No hit SEQ ID N°629 C171 GATCCATCCA AATGAGTCGG TGTTAGGAGA ATAGCTGATA No hit SEQ ID N°630 TACTAACTGCCTTGAACTTTG CCTTCAGCTT GCAGCTCCTC TGCATGTAGT GAGGAAGCTAATGCAGCTCC ATTTCCATGA ACCATAACAT TGTCACTTCG TGGGATGATATGTGCTTTGACCATGGTAGC ATGAGGGACA AAATTCTTCA TTGCGTTA C179GATCCCATTGTTGTCATAAGCGAGACAGAAGAAAAATATCAGT No hit SEQ ID N° 631CTTTTGAGGATTGTCCTGGTTTATCT C180aGATCCATTACAACAGATAAATTGCAGTGTTCTGTTGGCTTA No hit SEQ ID N° 632 C181bGATCCATATTCATGTATACAATACACTCATCTGGCCTTA No hit SEQ ID N° 633 C181cGATCCATAGGAGGGAAAGTCTGATGCCAGCGCCGCCTTA No hit SEQ ID N° 634 C183bGATCCATGAA AGCTAGGTTG AAGATTTGTA TCAAAAAGGG No hit SEQ ID N° 635GGCATGATGAATTGAGCATA AAGTTTGCTG CTTCTTGCTG ATGATAGGGGGGAGTGAGCTTTGGCTTGCG TTATTTGTCC TAACTAGCCA ATGGTCTTCTGGTGGCTTCTGGTGATTGGC TAAGTCAAAG CCATGGTAGA TTATTTGTTGCTGGATTGTGCTAAGTGTGC AGTTGGAACA TGTACTGGAG AAAAAACTTT GAGGTGTTGATTA C184GATCCATTGA ATTTCCAGAA GTGCCCTTAC AACAGCAACA No hit SEQ ID N° 636GCAACTGCCCCTGTTGCATA AAGAACAACG GCAGCCATCT GAGTCTTTGAGAGTAACAATTGAGGAAAAT GCTCCTATTA TAGAAGAGGG CCCTGCATC C185aGATCCATTCAATTTGTGGAAGCTGTGGTATATTGGACGTTTATG No hit SEQ ID N° 637AATGGTACGTTCCTTAGTTCTGCCTTA C185bGATCCATGGTTTTGTACTTCGTATGATTTTGAATTACATCTGCT No hit SEQ ID N° 638 GATTAC187a GATCCATAAAGTTACTTGATATGCCATCCTGTCCAGCTATAGA No hit SEQ ID N° 639GGAGTATCAAATTGAAGCATTA C187c GATCCATGGC CATTATTTTC GCTGTATTAC ACATCCATCANo hit SEQ ID N° 640 ATAAAGGTCCGATTTCTCCG TATTA C13 CACAAACAAATAAAGCTATT GTCATTCATT ACTCGAAAAA No hit SEQ ID N° 641 GAAAGTACAACATATCAAAG AGCGATGACA CAAATTATCA GTGATCTCCT ACTGATTCAC AAACCAACTT GTGTTAC14a GATCCCAAAG TAAACAAGCT AGCCACAAAA AGTGCAATTC No hit SEQ ID N° 642TTGATGTATA GCAGAAAACC CCTTGTTA C14bGATCCCACTGGAAGAAGCTGAGTTACTCAGGACTCATCAGGA No hit SEQ ID N° 643GGTGTGGCTGTGTTA C15a GATCCCATGA ATTGTGCTGT GACTCAGGAC TCATCATCAT No hitSEQ ID N° 644 TGACAGCTGC TGTTA C23a GATCCCAATT GTAAGTTCAT GTAAATGTACATCATCGTTA No hit SEQ ID N° 645 TTTTTTTGCA GGTGCCAAAT TTTCACATACAGCACCTTGC CTCGTATCTT TTGTCTGATC TTATATTA C29bGATCCCCAACCGCCATGTTGACTTGAATCAAACAAAAAAAAAT No hit SEQ ID N° 646TGAACAGTTACTAAGTACTTTATAGAGGGCGTTA C32 TGACTGCGTA GTGATCCCCC ATTATGACCAAGTTTGGCAT No hit SEQ ID N° 647 ACATTGTAAC TGAGATATCA TACACTCACATATTGAAGAG TTATCCTTTT TTAGCTTCAT AAATTGATTC ATTTTGCTTA CTCAGGACTCATCGTCA C33 GATCGACTGCGTAGTGATCCCCTCCTGCTGATGAAGTGACCGA No hit SEQ ID N°648 AAATTGCTTAGTGGCATAGCGAAAAAGGCAAGGCGCTTA C35 GATCCCCCAA AAATATACTATTTTGATGGA TTCGTCACAT No hit SEQ ID N° 649 ACTAGTAATA TTTTTGAAGAATTCGGGCAA CCTAGAGTAC GAGTGTATTT GTCCATTA C36 GATCCCCAAG TATACTCATGTATACGTGGA CGTCAAGTAA No hit SEQ ID N° 650 TAAAGTGACT CGAAAGTCAAATGTCGAACC CACAGATACT TACATTA C237a GATCCCGAAC ATTCGATTGG TGAGTTTATGCAGCAGATGT No hit SEQ ID N° 651 GTACAGTGTA CTTTGTTTA C204aGATCCCGGCCACTTTTTAGCTTA No hit SEQ ID N° 652 C204bGATCCCGACCAAACTTATACTTATGAATTAGTCCCTTA No hit SEQ ID N° 653 C205aGATCCCTAAC CTTGTATTAT GCGGCTGTGA CCCGGTTGAT No hit SEQ ID N° 654ATTTATGACC ATTTCTAGTG TGATTCCGTG TTA C205b GATCCCTGAC CACCGAAAACCAGCTCCCAT TCACCTCCGA No hit SEQ ID N° 655 TCTCACACGA AAACAGACCC CTTAC205c GATCCCTGGAGCTGCGAACACGCCTTATGCGTTCGGTCTATTC No hit SEQ ID N° 656TCAGTCCTCCTTGTCGTCCTAGGCATCGTGCTCATTGCTGTTGG CTTGCTATACCTCGGGTTA C206GATCCCTAGT AGGAATGCTT GTTTGCATCA CGTGCATTTG No hit SEQ ID N° 657ACTTTGGGGA CTCAACACAG GGGTTGGGTT CGTCTAGGAC AGGTGCACCC AAAATAACAGCTCCATCTTG A C207a GATCCCTAGT AGGAGCGCTT GTTTGTATCA CGTGCTTTTG No hitSEQ ID N° 658 ACTTAGGGAA CTCAACACAT GGGTTGGGTC CGTCTAGGAC ATGTTTACCCGAAACAAAAG ACCATCCTGA TGCATCTTAC CTGCTACGTG TGCATTTATT TGTTTCGGCTTGTTTGTTGA CCGGTTA C209 GATCCCTAGT AGGAACGTTT GCTTGCATCA TGTGCATTTG Nohit SEQ ID N° 659 ACTTAGGGGA CTCAACACAG GGGTTGGGTC CGTCTAGGAC AGGTGTACCCGAAATAAAGG CCATCTTCAT ACATCTTACC TACTATGTGT GCATTTATTT CCGGC C213aGATCCCTTTC TCTCAGCTTT CTCCCCCCAA GTCTTGAAAT No hit SEQ ID N° 660 GGTTAC216a GATCCCTAGTAGGAACGTTTGTTGTATCACGTGCATTTGACTTA No hit SEQ ID N° 661GGGGGCTCAACACAGGGGTTGAGTCCGTCTAGGACAAGTGTACCCAAAAATAAAAGACCATCCTGAGGTATCCTATGTGCTACATGCTGCAATCTTCAAGGGTGAAAAGGATCATTGGCGGATCAAT GATGGTTA C222 GATCCCTTTTGTAACGACCC ATCACGTGGT CGCCCCCTCA No hit SEQ ID N° 662 GGATAATGTCTATGCTTTCA AATGCTCTCT TTACTACTCC GCCTTACTCA GGAC C227b GATCCCTAAGCTTTTCACTC ACGTTAGTGA TAGGTGTTTA No hit SEQ ID N° 663 GATAGAGTGATTTGTGGTAG TTGAAGTTTG AGTTGAGGTT ATTTGAGCAA TGACTCATGT GTGTTTCTCCTTTGTAAGTA ATCTGCCTTG TTTGCTGCAG TTACATAGAA CTCACATTA C229 GATCCCTTACAAATGACCAG CTGGTTTCAG ATTACTCAGG No hit SEQ ID N° 664 ACTCATCATC ATTAC231a GATCCCTTAC AAATGACCAG CTGGTTTCAG ATTA No hit SEQ ID N° 665 C231bGATCCCTAAT TATTGATGTT TTTTGTTGAT TA No hit SEQ ID N° 666 C231cGATCCCTGGT CTGGGATTCT AGAAGTGCAT TA No hit SEQ ID N° 667 C302ATAATAGCTGAACAAAGTGATAAAAATCTATGTATCATAAGCG No hit SEQ ID N° 668GGGACTGCTCCTTTCAACTGGAGCTTTCACACCGCTGTATCTTCTTCAACATGTTCTATTCCCCTATTGGTTATTATAGTCCTGTGAGAAGCATTTTCCAGGAAATAGATCATGTTTTGCTTTA C313b GATCCGAGGG TAGTTTTTCGGTGTTTAGAT ACTCTATATA No hit SEQ ID N° 669 CTTGTTTCTC CAATCCCAAGAGAAGATCGT TCGAGTTCAA CAGTCAGGCG TCCACCTGCA GAATGCGAGT CAACAGTCCAAGGTTATCAA CAGAAGTTAG TCACAATAAA GAAAAAGAGA GACAGGCAAG AAGTAAATCCAAATGCAGAA GTTGATGAAA GATGTGAACT GCTTA C314aGATCCTGAAACTGGATATCGACTGATAAATTATCATCAACGTT No hit SEQ ID N° 670TTTGCTTGTGTACCATTTCTTTTCCGTAAAAGACATACTGCTTAGTTTTTATGGTCCTACATTCACTGGGGCATAGCGGCAGACTCC CTTA C315ACGGGGTAGCCTGATAGAGAAGGGACCGCTCTTAGAGGGATG No hit SEQ ID N° 671ACCAGGGAAGCTTATGCCCTTA C317 ACACATGCTCAAAGGAAAGGCGCGACCCCAGCGAATACCGATNo hit SEQ ID N° 672 GGAGTTTCTGCGCTCCAATGCACTCTAAGGACGTGGAAACTCCATGCTCGGGTATGGGCGAGTCTTGCATTACTCACAGACTCATC GGCACCATTA C318GAGAGATTGGAGGTCAACTTCGTCAGATAATCACGAGGAAAG No hit SEQ ID N° 673ACCAGCAACTACAAGAGACACAAATAGGTCATCAAGACGCAT GCCTAGCTCCTTCTTGTTCAGGCATTAC321a CGACTGCGTAGTGCTCCGAATTGGAGTATTTTTTTGCTAAGTTT No hit SEQ ID N° 674TTCTTTGGGTCAGAGCTTGTTGTCGCATTA C321b GATCCGATAG TAAAACCAAA TTACTCAGGACTCATCGTCA No hit SEQ ID N° 675 TTA C321cGATCCGAATTGAAGTATTTTTTTGCTAAGTTTTTCTTTGGGTCA No hit SEQ ID N° 676GTGCTTGTTGTCGCATTA C342 TTGAATACAAAATCAAGTAGCCGAAGGCTTTAATTGTGAGCCG Nohit SEQ ID N° 677 GTCAAGTTCAGCAATACTCAGCTGCGCAAAGCCGTAGAGTGGATCAAAATGCAACAATTTCCAGTACTACAGAACAATTATTTCC TGTACCTTCATTTA C343GATCCGCCTC TGGATCTAAG TGGATATGTA CCACTCCCTT No hit SEQ ID N° 678TACTAGGCAG AACCAAATTC TTCGCTAGCT GATAACTGGT CTCATTGTAT TTCCTCTTTA A C344TGTCGTTCCCCTTCATGTGGTTTCTGGGAGCCTATCTTGATCTT No hit SEQ ID N° 679 TAC347c GATCCGCCCTGGCCTGTAAGACTGAAACTACTTTTTGACCTAC No hit SEQ ID N° 680CGAGTAGAAGTCAAGTATCTAACGTACTAAACCCTCTTGTCAGTTTTTTCCTCGTTATTGATTCTCTTGTATGAACAGGACACTATAGACGCCAGTCCCAGTGATTTGATTTTCGACGCAAATCCAGCTCCACATATTGATCAAAATGGCATGGAGCTTCAAGAACTGAACAC CAGGCCTCATCTTGTTA C352aGATCCGCGAG AATGCTGCTG CTTGTTAGTG TCTGTTTGTG No hit SEQ ID N° 681ATTTGCATAG CTTTTGATAT CTTATCTTAT TGGTACCTGA CCATTAGTCT TA C355bGATCCGCAAG TATACTCATG TATACGTGGA CGTCAAGTAA No hit SEQ ID N° 682TAAAGTGACT CGAAAGTCCA ATGTCGAACC CACAGATACT TACATTA C356cGATCCCAAGAGTAGCTGCCTTTTAGACGGTGTGATCTAATCGT No hit SEQ ID N° 683GTGTTTGACTCTATTATGATACCTTCATCTGCTGCATTA C357a GATCCGCCTG GCTCCAAAGCAGAATTTTTG TTGAATCGGT No hit SEQ ID N° 684 TGTATGCTGT TGTCCGCATT A C357bGATCCGCCCCTGCATTCGTGTCAAGTTTCTAAAGCGAGTTTTCA No hit SEQ ID N° 685AATAATTGCTCTGGTATTA C335a GATCCGTCCCTATCCCTGCCTAGTCTATTTCTTTCCTGGATACTNo hit SEQ ID N° 686 GCATTTA C335b GATCCGTGGT TATGCCTCCA CACCTTCTGAAGTAAAAGGT No hit SEQ ID N° 687 CCCTGTTTTA C337GAAAGGATCACGGATTGGAGCTGTGTCTATCTTGTTATAAGGA No hit SEQ ID N° 688TTGTGTTGTAATAAATAAGTTCACATGGTTA C340GGCCCTTCTTCTTGCTATTTTATTGTTAGCTGATATTGCTGCTTT No hit SEQ ID N° 689GATTGGCTTTCTAAAAATTGTAAAATGCATATTCACGCTCGAATTTTCAGAGATGTATTTTGGGTGATTGCTTTGTTTATTTTGAGA AGTAGAGATATTGAATTCCACCTTAC368a AACCGGAGATGAATCAACGACGAACTTTGATTGTCCACAAATT No hit SEQ ID N° 690TGTCCGAGAACGAATCTCTCACCAAGATAACTTGACGTCGAAAACGACTACGAACGGACGACCAAAGAAGGTGGTCGTTTGGCAT CGTTTA C405ATCCTTTCCTTTTTGTTCGCGTCATGTTTCAACCGAGCCTAATA No hit SEQ ID N° 691GTTCTAGGATTCGGTTCTTCTTTCATTAGTTCCCCAAAAATCTGAATTTTACTACTAAGAACTTCATACGAGTTGGTTTA C406 GATCCTATTC GTACGTTTTTTTGAAGCCAT AGTACCAGAA No hit SEQ ID N° 692 TCTATTGTCA TAGGTTTTTTGAGTTTGTTT TTCTTTTATT GCTGTTAGAA TCATATGTTC GGGTGTGACT AAGATAACTGCTTAGTGTCT TTTA C411a GATCCTAGAG AGAGAAAGAG AAAGAGATAG CAGTTGAGTA No hitSEQ ID N° 693 AAGGAGAGAG TCCTGTTTGT TGAAGCTGTA ATGTAAAACG CGTTCTCCCCCTTCCCGCTC TGCTGGTTA C411b CGCGTTGGGAGCTCTCCCTATGGTCGACCTGCAGGCGGCCGCGNo hit SEQ ID N° 694 AATTCACTAGTGATATCGAATTCCCGCCGCCGCCATGGCGGCCGGGAGCATGCGACGTCGGGCCCCATTCGCCCTATAGTGAGTCG TATTAA C414aGATCCTGGTGTATACGCTTCACCTCGTCCAAGATACTACTGATT No hit SEQ ID N° 695GTGGAAAGTGCATGAAAGTCAAAAACACTACTATTTGATACTCACTTGTATTGTTTTACTATAGAATCAAATGGTGTTAGTATGAAG TGAGGGGCTGCTTA C414bGATCCTAACA CAAAGATTTC GTGATGGTTT TGACCTATGC No hit SEQ ID N° 696TCGCAACCTT AGACCTCAAC CTCATTGACT CTTATCATCA GTGTATTGTG TTGTACAAGTATGTGATTCT ATTATCACAA ATGTGTTTCA GTTTCTCCTT TTGCTTA C415 TATATTGGGCATTGGGTCGC ATGTTGCAGG CTGCCATGCC No hit SEQ ID N° 697 CCATGGCTTCGGTGTGTAGT GATCAGAATT CATATTAGGT CTCAACAATG TGCAGCCTGC TATGTAGCCACAAATGACTT ATAGCCGCCT TA C416AAGCTCGGTGTGAGAGCATACACTGGTGCTCATTACTATGTAC No hit SEQ ID N° 698TCTGGCTTA C417b ACTAGTGATTGATGACCCCTGAGTAAGGCGCTTTCAGTGAGAT No hit SEQID N° 699 TCAACAATTAGGACTAAGCGTTACACTCTAGGATCACTACGCA GTCAATCCCGCG C427aGATCCTCAAG CGAATGGGGT CTTCTTGTTG TTTACAAGAG No hit SEQ ID N° 700TAAGGGCCCA GAACTTTTTA GCCACCATAG TTGTTTA C428cGATCCTCCAAGCAAAATAATTGAAAAGGAGGTGGTAGCTGGT No hit SEQ ID N° 701CCATCCTTTA C433a GATCCTCAAAGTTTATGTGTTGTTTATTTATATCATTTTTTCTCG No hitSEQ ID N° 702 ATAGTTA C434b GATCCTCATTCATGGAATGGCTTGTTTCTGAGCAATTTGTTGCTNo hit SEQ ID N° 703 GTACCCACTTCACCGCTTGCAAAAGACATGAGCCTGTTGGAAAAAATTTACGATTCTATCCTTGTGATGGTGAAAGTATTCATTTATGATAAATCTACCACTTTTGATTGAATTTCACGATCCAAAATAAAGGATGGTGTTGCATACTATAAGATTTTAGTTTGGAGATCGGT TTCCCTATTGATCTTA C435aGATCCTACAT GAACGTGAAA TGCATTGTAC GTAAGGCTGC No hit SEQ ID N° 704CATTTTTTTT TACTTTCTTG TGAACCTACT AGGAAGTTGG TTGTGGACTT TATAATATGATTCCAAGAAG ATAATACTGT TGAAAGCAGC GGGGGAAGAT CTACCAAGCA ATGCATAACAAGAAAAGGTG CCTTA C437 GATCCTGTCCTGATGAAAAGTCATTGGGAATAGTTCCATGTAC No hitSEQ ID N° 705 AATTGGCAATTTGGAGCACAATGAACTGGATTCATGTACTAGTTCTGTTTCGGCCTTA C440 GATCCTGATA AACCAACATT ATCGTAGAGA ATTTTTCTCT No hitSEQ ID N° 706 GTTTCTCCCT CTGAAGAACT TGCTTA C451b GATCCTGGTG TATACGCTCCACCTCGTCCA AGATACTACT No hit SEQ ID N° 707 GATTGTGGAA AGTGCATGAAAGTCAAAAAC ACTACTATTT GATACTCACT TGTATTGTTT TACTATAGAA TCAAATGGTGTTAGTATGAA GTGAGGGGCT GCTTTA C463bGATCCTGCTTTCCACTAAAAGCTTGTGAACTTTTGGCCTAAACT No hit SEQ ID N° 708CTTTGTTGCTCAATGATATCATCTGCTTA C468CGACTGCGTAGTGATCCTGCAGTTGATCCTATTGCTTATACAA No hit SEQ ID N° 709GCCTTGTTTTTACTGTCACTTTCTTTGCGGGTACATTCCAAGCT GCATTTGGCCTATTA C470aGATCCTTGCATGTTAGTTTACAATATTCTCAAATTACTCAGATG No hit SEQ ID N° 710TAGTTTACTTTTTCTGTTTCTTTTTCCTCTAGTAAGTATATAAGTTATTTGTTGGAATAAACTCTAGAATGCTTGCTTCTTTATGGCAT ATATTAGCACCTACTTTA C470bTAAACCCAAA ATTGAAAACC AGCTGACACT ACTCGAGTTT No hit SEQ ID N° 711TTTGTTTTTT TGTTTTCTAG TTTTGAATAT CCTATCAGTA TGTGTATTTT CAGTATTTTTGATGCAGAGA AAATGAGTTT TCAAAATCTG GTTTTCTAGT GAAGGAAGGA TC C473GATACAACGTGATATATTGACAGAATTGTGTTTCGGTTATCAT No hit SEQ ID N° 712ATAAACATTATATAGGTTCTGCTTA T114b GATCTACCG TGTGTGCTTC TTAGCCTATTGAAAATCGGA No hit SEQ ID N° 713 TTGCATTTTG CTCTAGGCTT ATGATCTTGTTTTAGCTTGC TCCTATTGGT GTTTATTTTT TACTATGTTT TATGTATTA T117b GATCAACCATGTGTGATTCT CAGTAAATCC GATTGCATAA No hit SEQ ID N° 714 TATATTTTGGATAGTTTA T120 AGTTTGCTTTACGAGATTTCCTAGTTATTATCCTTTGAGTCTGT No hit SEQ IDN° 715 TGTTCTTTTTTATATCGACTTTTACCTTCTAGTTTTGCACAACAATGTCTAGCTTTTTTTGTTATTGCCCTTTCTATTTTGTATTTGAAA AGGTGTGTTA T125aGATCTACCAACTCGGGGGTTTATTTACTGTCATTCGTTACTCAT No hit SEQ ID N° 716GACTCATCA T125b GATCTACCAACTCGGAGGTTTATTTACTGTCATTCGTTACTCAG No hit SEQID N° 717 GACTCATCA T131 GATCTACCGTGTGTGCTTCTTAGCCTATTAAAAATCGGATTGCA Nohit SEQ ID N° 718 TTTTGCTCTAGGCTTA T136ACTAGTGATTGACTGCGTAGTGATCTACGTTGCGTTTGGTTGG No hit SEQ ID N° 719ATGAAAATAGTTGTGGCATACACTTTCTTTTCATGATTTTGGAT TA T138a GATCTACAAACTTGCAGAGG TGAGAGCAAC ATGGATTTAT No hit SEQ ID N° 720 CCTTTTCCTTGGATTATTTA T138b GATCTACAAA CTTGCAGAGG TGAGAGCACC ATGGATTTAT No hit SEQID N° 721 CCTTTTCCTT GGATTATTA T141b GATCTAGTAT GTAATTTCTC TAGTACCATATTTGCGATTT No hit SEQ ID N° 722 TCCCATTATC TTTGTTTGTA GTCTGTATATTATAGTAAGA AATTGAATAA CAAAAGACAT AGAAA T149bGATCTAGAAATATATACCTTGGAGTTTCAGAGCTAACACACGC No hit SEQ ID N° 723AGAATTGGGGTTGTAAATAGTGCAAGTAGCAAATCTGTAATAATTGTTTAGTGTACTCATCACCCTTCTGCTAGTTCAAAGTGGCTCAGTTCAATACAAATTCAAAACTTTTGTTA T160a GATCTGATAT TGCAGGTTTA GCCAAATCATGGTCTCTCTT No hit SEQ ID N° 724 GGGCTGGCTG GAGTCCTCCG ACCTAGATNAAGTCCCTGAC TGCGTAGTGA TCTAGGGCGG GTTCTGTTGA TGTGTACATA TAATAAGATCACGTCTAGAT TATGGATTCT CTTTGAGGAT AAGTTTTACT TTTTGTTCCT ACCTTTTTGT AGTAAT169 AATTGGTGGACAGTATTATAGGCTCAAATATAGGCGAATGCCT No hit SEQ ID N° 725TCGAGCCCCCAACTGCACTGAAAGTCAGAACAATGACTTCAAAGGCACCCCTTGGAACTATATACAACATGTGCAATGCAAACTTGTGTTTGAGTGTGAAATACCATGGATGCAAGTTATCTTTTGAGCTTACTCTTCTATTTCATTCATTTCTGTAATGTCCTGAATACAATCTTATATTCTGCCTAGTAGAGAAGCCCTTCCTCCCCTCTCTTATGT TGTTA T173CGATACTCCAGCAAAGAAGAGAAAAAGCCAGTTTTGGCATCA No hit SEQ ID N° 726AGGGTTCAAATCGAAGTTCCAAGAGTAGTATTTTTCCTCAGATAACTACTGATAGTGATCTTTGGGTGGAGGCTCATATTTAGAGGGATATCTTTATCTAGCACAACTGGATGTCACACTGATAGTGATCTTTCTTGGGTTGTTCTTGTGGAGGAAATTCACCTTGCGATTC CTTA T174GGTTCATACAGTCCAAGACTTATGTGATCTAAATCCAGAATCG No hit SEQ ID N° 727TAGTAGCTGATTCAAAGTCGCGTGAACAACTTCTTCCATGCTCCCAGACTGTACAGAAACTGTTGCAGACCTTCACCTTA T177bGTGCTCTATCCCCACAAAATTCCATTTTTCTTCACCTTAGCTTC No hit SEQ ID N° 728TTTATTTTGGCCGTAGAAACCAGTAGCTCATAGCTATGTGAAC CCTCTTCCCTTACCACCTTA T7GATCTCACCC GGTGCTGCTC CAAGGCAACT CAATAATCAA No hit SEQ ID N° 729AGAAGAAAAT GAAGTGGTCC TCTTGCTGGA AATACAATTA CTGTCGTTTC GATTTA T10GATCTCATCT CAACAGCGGA CATGAACAAG CACATACTTT No hit SEQ ID N° 730GCCCTAATAT TGAAGTGGAC AGTTGGTTA T26 GATCTCCATC GATCGAGTCA GAAAGATCATTGTACATGTG No hit SEQ ID N° 731 CCAATTAGTA ACCAGTGTTT AGATCAACTATGGTGTTATT TTTGGGTCTT ATGTTGAATA ATTATTTGAA GCTTTAGTAC ATTTGATGTTGTAATTGTGG AGTACTTGTA TTTTTTATAC AATATCTTTT ATGTTTA T31GATCTCCTACAGTCCTTGCACGTTTATCTTTTTGTTTCTTCTTTT No hit SEQ ID N° 732TGGGATTTA T34 GATCTCCTCCAAAATCCTTGTAAGAAATAATGCTACAAGCTTA No hit SEQ IDN° 733 TGAATCCATTTTCTGGTTA T40GATATTAAAATGAGGAGATTTACCACTCTCTTGACTATGTATA No hit SEQ ID N° 734CTAATGAAATTATCTCCATATTGAATGGGGATGTAATACCTTT GTCTCTTGATTACTCAAGACTTATT202 GATCTCGGCA TGTATCAAGT CAAGACCGGT TGATTAGCCA No hit SEQ ID N° 735ATCAGGAGAT TTCCTTCTGT ATTTA T206b GATCTCGGAG TGAATATGGA CGACGACTACTTACTGCGAA No hit SEQ ID N° 736 ATGCTAGTAG TCGTAATTCT TCTTCCTCTGTTGATGCTGT GGAGAGAGCT AGAGCGTGGG GTGAAATGTA TTTC T209GCTTGAAGACTAACTTGGAAACCATGCTTTCGCCCTCTAACCC No hit SEQ ID N° 737AGCTTTTAACCAAAACTGTCTGGAACAGCTGCTTTCAAGCATCAAGAATTGATGATGCCCCCCTTAGGACAGCAACTGGGCCCCCGGTAAATGGAACGGGCTGGAAAGAAACAACAATAGCAACTCCTTCTAAAACCCCAGGGAAAGGGGAGATAGAAAGACTATTCACTACAGCGGAGAGAGTCTATTTGATGGTAAGAGCTATAGGAGCC CTACTTA T218a GATCTCTGGTTCAAACTAGA TTCTGGTTCA ATTTTGGTTC No hit SEQ ID N° 738 GCTTTA T218bGATCTCTTGAGAGAGAAGTCGTATGGTCAGTGATTTCCAGTTA No hit SEQ ID N° 739 GTTTAT219 GATCTCTAGT AGGAACGTTT GCTTGCATCA TGTGCATTTA No hit SEQ ID N° 740T223 TTCTCCTTATCATCACTAGTTTAGTTCCATTTGTACATACCTTTT No hit SEQ ID N° 741GTAATTCGCGGGGAGAAAATTGGATAGGTGGATTACTAAGCAT AACACTACTGTATCACTTA T224ACACATTGTTCGGAGCCCAATTGCTGTGAGATTCCTCTTTTTCT No hit SEQ ID N° 742AGAAAAAGGAAATGATGGCGCTAATCTCAGCGACATGCTGATTTTTCATTTGTAATAAAACATTTTCACATCATTTTTGCTTA T229bAGTTGGACGATGGGTTGAAGACGAAAGCAGCGGCGTGTGGAC No hit SEQ ID N° 743TTTGGATGTTCACTGAATGACGAGGCAGCAGCTGCTCGTCGACTTAGGGATTGTCTGGAGAAGATGACTAACGGGGGGGGTCGTTTTTGTGCTAGAGATCACTACGCAGTCAGGAAGTGACTGACCCC T230GTGATCTCTCTTGAAATCTATAATGAGACGTTGACAGAAATAA No hit SEQ ID N° 744GCAATAGATGTGTTATGAGATGTGTTTTCCGCTCTCATTA T231 GATCTCTATT GAATATGGAATTGAAGATAT GATTTGTTCT No hit SEQ ID N° 745 TGTTGTATTT ATGTCCAGATTTCGTGTATT A T303a ACTAGTGATTGACTGCGTAGTGATCTGATTCAAGAGCGAGGAC No hitSEQ ID N° 746 TGCTGACTTCGTCTTGCTTTTGGTCCATTCAACTCGTTTA T304bGATCTGAGGTTGCTGATTTAGATTATGATGACTTTGAGGCTGA No hit SEQ ID N° 747CTTTCAGGTCTTTA T306 GATCTGATAACAGGGTTGGGCTAAAAGAAGCCAAACAGTTGTT No hitSEQ ID N° 748 GATGGCTTAGCTAGAATATAGGTTATTGAAAGTTT T307a GATCTAGGTGCTTCTGGATA ATCTACACGA ACTTCTGGTT A No hit SEQ ID N° 749 T307bTGACTGCGTATTGATCTGAGCAAGCAGTACAGTACAATGATTG No hit SEQ ID N° 750GAATATTGTTA T307c GATCTGATGC TGAGAAATGA GACGGGGTCG TTTGGTAGTT A No hitSEQ ID N° 751 T308a GATCTGATAT TGCAGGTTTA GCCAAATCAT GGTCTCTCTT No hitSEQ ID N° 752 GGGCTGGCTG GAGTCCTCCG ACCTAGATGA AGTCCCTTCT GATAACGGAGTTGGTGTTCC TGCTTGTGCC ACGGAGCAAG GACTAGACTG ATCAATTGCA TTAGTGGATTCAAAAATTTC GATCTCACTG ACTGAATCAA GAAGCATGTC AAGCT T310a GATCTGACTACTCTGGATTC ATTACTAGTA ATATGATTT No hit SEQ ID N° 753 T310b GATCTGACTACTCTGGATTC ATTACTAGTA ATTGATTTT No hit SEQ ID N° 754 GTATTGAAAC CAGAGAGAT313b GATCTGATTCATGTTCCCACATGATACGTAGGCAACCCACATC No hit SEQ ID N° 755AGGTTCGATCACCATTA T315c GATCTGGCCG GCTAAACTAA TATTGGCGCC ACCACTTCCT Nohit SEQ ID N° 756 CAAACTTTAT CTTCTCTGTT ATTGGCCAGA AGAGAGAGAC CTGGCTGGATTTTTACTCTT TCTGGCAGTC TTTGTGATTT TCTCTCTATG ATTCGCTGGA GAATATATTTTTCCAGTGAC CTTTTGTGCA TGATATTTA T316a GATCTAGCAT CCAATGGACC AAGTTCTCTGGTGACAGCCA No hit SEQ ID N° 757 ATTATTGTGA ACGCTTCTTC TGGTTGAGTTGCTGGTTATA CTAGGTAGTT CTCTGTATAC CAGCTCCTTG TTA T316b GATCTATGTGGCTATGATTT TTGCTTAGTA GTTGAATTGT No hit SEQ ID N° 758 ATTATTTTCATTCTGCAATC AAGTACTGCT ATCTTTTATT TCTCGGTTTT CATCAAATGT TTGCTCTTCC TTAT317a GATCTCATGA ATGCATAGTA GAATACTGTT GTCTTTGCTT No hit SEQ ID N° 759ATGTTA T317b GATCTGGGGT TCGCAGCAAC TTTTGAAGAA GAAGAAACTT No hit SEQ IDN° 760 AGGTTTA T322b GATCTAGGAGTTTGTCATCTAAGTAAATCAGTTTTGTATCCTTG No hitSEQ ID N° 761 ATTTTTGCCATACAGAGAGACCAGGGTAAAATGCGCTTA T322cGATCTGTGTGCCTAATGATTTTTGCCTAGTAGATGGATTATATT No hit SEQ ID N° 762ATTTTCATTCTGCAATCAAGTAATACCTATCTTTTATTA T324b GATCTGGTGG CTATTCTTCCTCAAGGTCCT CGATTA No hit SEQ ID N° 763 T324c GATCTACGAA ACGGTGTGTTGTATTCTTGT TCATTA No hit SEQ ID N° 764 T325a GATCTGGTAA CGGATTTGGCTCCGTTGGAG TCGGACAAAA No hit SEQ ID N° 765 ACACTCTCAG CTTTA T326aGATCTGGGCA TGAGGTTCGC AAGCATGTCA TGTTATTGAA No hit SEQ ID N° 766ATCTGCATTT A T326b GATCTGGGCA TGAAGTCTTC AAGCATGTCA TGTTATTGAA No hitSEQ ID N° 767 ATCTGCATTT A T332a GATCTGGGTC TGTCAATGAA GAAGAAGAGCTTAGGGCATG No hit SEQ ID N° 768 TGTGAGGTGA AGTTTCATTT ATGGCTATTGCGTAGTGAAG GGAGGTCCGA CGATGGAGTT TTGGTGGTGA GGGTGCGGCT GGTGTGAAGATGGAGCTAGG TTCTAGGTTT TTTTTGGTGT TA T333a GATCTGGGTC TGTCAATGAAGAAGAAGAGC TTAGGGCATG No hit SEQ ID N° 769 TGTGAGGTGA AGTTTCATTTATGGCTATTG CGTAGTGAAG GGAGGTCCGA CGATGGAGTT TTGGTGGTGA GGGTGCGGCTGGTATGGAGA TGGAGCTAGG T T333c GATCTGATCC AGCAGTTGAT CTAGCATTTCATATTCAGTG No hit SEQ ID N° 770 TAATGACTGC GTAGTGATCT GGGTCTGTCAATGAAGAAGA AGAGCTTAGG GCATGTGTGA GGTGAAGTTT CATTTATGGC TATTGCGTAGTGAAGGGAGG TCCGACTATG CAGTTTTGGT GGTGAGGG T335a GATCTGGTGC GTAGTAACCTGTGCTTTGTT CGAATTCGAG No hit SEQ ID N° 771 GTGCAATCAC ATTCAAGGAAAAATAATATA ATACAAACGA CTTTTTCTTT TTCTACCTTG CTTCAATTTT TACTTCGTATATCATAAATT AGTGGTTTAT TTGTTATGTT TCATCACGTT TTGATAATTT TATTGATTA T336bGAATCCACCTACCTAATAGCAAGAACAATTGAATTTGACCGAA No hit SEQ ID N° 772CAGAGTTCTGAAATTGAGGGGAAGCCCCAACACCGTCTCCCTC CCCGCTAATTCCATTTCTCTAAATTACAT338a GATCTGGGGAAAAAGGGAAACAAAAAAAAAAGCAGAGAAGG No hit SEQ ID N° 773AAATCTGCCGCCTCCCTAAATGACTAACTCCTCCTTA T338b GATCTGGGTG AAAAGTCAGAAAGCAGCAAA GCAATGTTCT No hit SEQ ID N° 774 TTTCTTCCCA AGACGCAACAACTCAATAGC CATGAAAACG CATTGCTTA T338c GATCTGGGGG AAAAGCCAGA GAGCAGCAAAGCAACGTCCC No hit SEQ ID N° 775 TTTCTTCCCA AAATGCAGTA GCTCAATAGCCATGAAAACT CACTCCTTA T343 GATCTGGAGATGCAATTTTTGATAACCAGCAGTTCTATTCAATTNo hit SEQ ID N° 776 TTGTGCAGTCCTTGCTGGTTGTTTCTTTCTCCATTTTTTTTTGTTCTTGTGAACCATTA T345 ATTGGGCTCCACTGCTATAGGCGCCTGCTGCAGTTTCGGTATC No hitSEQ ID N° 777 AGACAACTTGTCTGATTTTGATGGCATTACTCAG T346 GATCTGGAGATCAGGAAATG TTCTAAAATC TCCCTCAATT No hit SEQ ID N° 778 ACGCTCTTGGGCTCTTGATT TTAGGTGCTC TTGGTCATCC ATTA T350a GATCTGTTCA AGTTGGCCGATTAGTCCATC CTTTTTACTG No hit SEQ ID N° 779 AATAACATAC AACTTTGTGCTTCTTTTTAC ATGAATAAAA TACTAGAGAT GTCTTTTCTC AACATTGTT T350b GATCTGTAGAGAAGGCTCGC TCCTAAATTA TATTTCTTTC No hit SEQ ID N° 780 ATTTACCTTTTCTTTCTGCT AATTTCCTTT TCCGTGGTCT CTTTTACTTT TTTCTTGGGA GGGGAAGATGGGAGTGGGGT GTCACCTTCC CTTGTC T350c GATCTGTGAG ACTTAGTAAG AAGCATGGCTGGTTTTTCAT No hit SEQ ID N° 781 ATGTACAGCC CATCTCATTT TAGTGTAGAATAAGCATGAG GTATGGTTCA TACGCTAATA GCACATTGAA TGGTAAATTT TAGGTTTCC T351bGATCTGTAAA TATGTTACAT ATTAGGAGTA TAATGTTTTC No hit SEQ ID N° 782ATTACTAAAG CATGTAAATA TGTTGCTCCG GGCTTTGGTC TATTAGTAAG AGCGCAATGC GTAT353a GATCTGTCAT TGATGTTCAT TACTCCAATC TTTTCTCTGA No hit SEQ ID N° 783CATGTTTA T358 GATCTGTAAA TATGTTACAT ATTAGGAGTA TAATGTTTTC No hit SEQ IDN° 784 ATTACTAAAG CATGTAAATA TGTTGCTCCG GGCTTTGGTC TATTAGTAAG AGCGCAATGCGTA T359a CCATCAGCTAAATTATCTCGAATTTCAATAGTGGTACTCAC No hit SEQ ID N° 785T359b CATCTGTAAA TATGTGACTG ACTGCGTAGT GATCTGTGGA No hit SEQ ID N° 786AACTGCTGAT CCGGTAATTC TCAGAGA T359c GATCTGTACT GTACATGTCA AAAAGGGAC Nohit SEQ ID N° 787 T360a GATCTGTGAG GGG No hit SEQ ID N° 788 T360bGATCTGTTGA GAAATATGCT AATAA No hit SEQ ID N° 789 T360c GATCTGTCAAAGGCCAA No hit SEQ ID N° 790 T364b GATCTGTGGA AAAGGAAAGC TGGAGAAACTTGCTGTGCTG No hit SEQ ID N° 791 TAATTTATGT ACAGTGCTAT TTGGCTGCTCAACTAAGATT GTTTTGATTC TCTCTTAGTC TGATGTTATC TTTTCTCGTG ACAATCCTTCCTTTTTCTTT CTTCTCTTGG AGTTGGGGGG TCAATATCCT TTGTTTGTGG TG T366aGATCTGTCGA AATTTGATTC TCGCACGAAT TGTACCATTG No hit SEQ ID N° 792CCCACTTCCA TTGGCAAGGT TAGGGTACAG AAAGTCTTTT GTGAGTGACC AATATATA T366bGATCTGTGAA TATGTTGCTA TTATATTTAC GCACATCTTA No hit SEQ ID N° 793GATTTCGCTT TTCTTTCTGT TCTGAATCTC T T371TTTATCCGCACGAGGCTTCTGGAATGTAATGGCAGCTGATACA No hit SEQ ID N° 794TTGATGTAAATGTAATGCATTGTGCTTCTCAACCAAAGTACAC TTCCGGGGAGGTCATTA T372aTGACTGCGTAGTGCTCTGTGAGAGGCCATTTGGATCATATATG No hit SEQ ID N° 795TTGCTATCCATTGCATTA T462 GCTAAATGATTTCTAATGGGATGGGCATGCTCCCCACTGCTCT Nohit SEQ ID N° 796 ATGATTTATTATAGTCATACTCTGTTTCGTACCTGGCCGCTAGCCTTTCGCTTCCTCCTTGTACTAGATTTTGACCTTGAATTCCCCCTGAAAGCGAGAACGCACTATATGCCTTTA T403a GATCTTATAG CTAGATGTTG GGTTTTGACAATTGAACTCT No hit SEQ ID N° 797 TATCATTGTA TTTGAGTTTG GACTGTCATGATGAAACTTG ATGAAAACCT GCTTAGTCGA ATCAGTAGCA AAAT T403b GATCTTGGAGTGAATATGGT CGACGACTAC TTATTGCGAA No hit SEQ ID N° 798 ATGCTAGTAGCAGTAGTCCT TCTTCCTCTG TTGATGCTGT GAAGAGAGCT AGAGCG T403c GATCTTAGAGTGAATATGGA CGACGACTAC TTACTGCGAA No hit SEQ ID N° 799 ATGCTAGATAGTCGTAATTC TTCTTCCTCT T466a GATCTTATAT GAGCTATGTC AATTTTGATC GGCTTCTTCTNo hit SEQ ID N° 800 GGATTA T466b GATCTTATAT GAGCTATGTC AATTTTGATCGGCTTCTTCT No hit SEQ ID N° 801 GGATTA T429a GATCTTCCAG GATTATTATTGTCTTCCGCT GCGTGTTACG No hit SEQ ID N° 802 AACACCTATA CGCAATCGTACATTATGGAC CATAAAACCG ATCCCCCTAA TCTTGAATAA AAAATCCATG CTATTTTTTGTTGTCATTCC ATTTA T429b GATCTGGCTGATAGTGCAAAAGATTCAACTATTATTGACATAT Nohit SEQ ID N° 803 GTTGCAACATGTACCATGTGTGGTTTGATCATGGCGCCTAGATGGAAGTGATGCTATAGTAAATAGACTTCACTTGTTTCATGCT ACTTA T432b GATCTTCAACTATCTCAACT GCTGTAGTGC AAAAGCTTGA No hit SEQ ID N° 804 AGTTCATGGGATTGATTTGT TCCAACTTGT TTGTAATGAT AAATATATCA ATGTGATTTC TCCTATATATGTTTTGAGGG ACTTTTCCAA GAAAAAGGAA AAGTGTGGAT TTTATGATTG TGGTGACTGG TAATTAT432c TGACTGCGTCTTGATCTTCAACTATTTCAACTGCTGTAGTGCAA No hit SEQ ID N° 805AAGGTGAACTTCATGGGATTGATTTGCTCCAACTCGTTTGTAATGATAAATATATCAATGTGATTTCTCCTATATATGTTTTGAGGGACTTTTCCAACAAAAAGGAAAAGCGTGGATTTTATGATTGTGG TGACTGGTAATTA T433aGATCTTCCAG AACAGCCATC CACC No hit SEQ ID N° 806 T433b GATCTTCCAGAACAGCCATC CACCAGTGTA AACAAATACA No hit SEQ ID N° 807 AATCAAGGTCCCAATGATGA ATGTGTTCA T436b GATCTTCCAAAATACAGCTAGGAACTAACCACTCAATAGATCANo hit SEQ ID N° 808 TCTCCAATAAATTTTGCGCCTTCCTTCCTTATTA T437TAGGGAATAGGAAGATGTACAAGAGGCAATATGGAGCACAAT No hit SEQ ID N° 809GAACTGGATTCATCTACTATGTTCTTTCGGCCTTA T438a GATCTACATG TCTAATGTAGTTGGGGATTT ACCTTATCCT TA No hit SEQ ID N° 810 T438bGATCTTCTGCAAAGGTAGCAGCTTCCTACTAACCAGATATTA No hit SEQ ID N° 811 T411aTGTATTTCTGCGGCGGGGGGGGGGGGACCTTTGAAAATACCAA No hit SEQ ID N° 812AAACACCCCTTATTTGCCCATTGATTTTGGTTTTAAAAATCA T411b GATCTTGCAT GAATACGGAATATATACTTT GTGCACCGAA No hit SEQ ID N° 813 GTGCCCTTCC CTTCTTGGTT GCTAT416 GATCTTTGGGTTCTCATGGATCGTGGGGACTATGAACTTTGAAA No hit SEQ ID N° 814GGGCTTTA T417 GACTACTTACTGCGAAATGATAGTAGTAGTAATTCTTCTTCCTC No hit SEQ IDN° 815 TGTTGATGCTGCGGAGAGACCTAGAGCGTGCCGTGAAATGTATTTCTTGCATAACTATGATAGGTTGGTTA T422aCATATGGACCAAACTTGTTCTGAGTTTTTGCTAGATTGAGACTG No hit SEQ ID N° 816CATGGTCCTCTC T422b GATCTTGCCA TGGACTAATT ATCAACAGCA GCCATATTGG G No hitSEQ ID N° 817 T426a GATCTTGGAG TGAATATGGT CGACGACTAC TTATTGCGAA No hitSEQ ID N° 818 ATGCTAGTAG CAGTAGTCCT TCTTCCTCTG TTGATGCTGT GGAGAGAGCTAGAGCGTGGG GTGAGA T428a GATCTTGGAG GAATAGAAAG AGCGTTGTAT ATTGCTCGCA Nohit SEQ ID N° 819 CTTTCTATAG TTTTGATTA T428b GATCTTGGAG TAATACAAATAACGTTGTAT ATTGCTCGCA No hit SEQ ID N° 820 CTTTCAATAG TTTTGATTA T441bGATCTTTGGACAAAGTTTGGGGAAATGATTGCTCTGCTCTTT No hit SEQ ID N° 821GTTGTTGGTTA T450a GATCTTTGCATAGTTCGGAAAAATCGAGAATAGACTAAATAAA No hit SEQID N° 822 CTAACGTTCTCTTTTTTCTTTCTTTCTCTTTTTTTTTTTACCTTA T450bGATCTTTGCGTAGTTCGAGAAGATCGAGAATAAAGGAAACAA No hit SEQ ID N° 823ACTAACGTTCTAATTTTCTTTTCTTTTTTTTTCTTTTTTTACCTTA T452aGATCTTTGCGTGTTGCACTACAGATTTTTAGGACCTTCTGACTT No hit SEQ ID N° 824 GTTAT452b GATCTTTCAG TGTTGTACTC TTCCCTGCTT TA No hit SEQ ID N° 825 T456GATCTAAGTAGAGCAGGGTTCTAGATGCCTAGGATGCTTTCTT No hit SEQ ID N° 826GGGTGAATCTGCCTTTTCCTCTTGCTGCCTATCTCTGTGGCAGCTCCAGAGAATGGTGATTGTCTGTTGTTTGAAGCTGCATTA T459aGATCTTTGATATTGGTAGCTTGTGAGTTGAAGACTAAGGCTTA No hit SEQ ID N° 827TTAGTAAAAATAATACATGTATTAGCCTTTGTATTA T459b GATCTTTAGA GAACTATAAGTTTTACTTCT GTTTCTTGAC No hit SEQ ID N° 828 CGTTTTTGAT TTTGTGTTATTGAGATATAC TTGCAATTAC TCAGGACTCA T460aGATCTTTACTTGATTGCTACTCCTTGTGTCGCGTCTTGATTA No hit SEQ ID N° 829 T460bGATCGTTACT TGATTGCTAC TCCTTGTGTC GCGTCTTGAT TA No hit SEQ ID N° 830MC103 TAAACATGCG GAAGTCCAAA GATAATACCA CTACCTAGCC No hit SEQ ID N° 831CACATTGATC CGGTGTCACA AGTCAAGAGC CTCTAATACA AGTCTGAACG ACCTAATACATAAATAATCT AGGAATGTGG AAAGTAATAA GATATGAAGG AGCAATCCGG GTCTACGGATTACATGCAGC TACTTCGATA ACTCCGGCAA ATG MC114a TAACGTATCA GCTTTGTTTTTTCCACGGTT CCACCTAAGT No hit SEQ ID N° 832 AGCTATGTTT CTTGGATC MC114bTAACTAAGGGAAAGAAGAGAAGAACAGAAATGACCTATAGCT No hit SEQ ID N° 833ACATTAGGCATGGATC MC119 TAAGAGGAGT GCAGCTTTTG CTCAAGTTTC AGATTCTCAG Nohit SEQ ID N° 834 CCCATAACAC ATCCTGAGAC TCTTGTTTGT GAGAACAAAC AACTTCATTCTGAAGGAGGG GTTCCCAGTA TTACCAAGGA GCAGTTTGAT CAGCCTTTGA CTCTTCTTCAACAGTCCAAA GTCTCAC MC130c TAATGAGGATGTGGTGGCTCTGTACAAAAGGTAGACTGATTGA Nohit SEQ ID N° 835 GAAGTATCAAACAGCTCAAGTGTAGATGTGGTCATCTAACAAA TGGTGGATCMC202 TAAACATACAATGACTGGGCTGTTATAGCAGGGGTTTCGGGAC No hit SEQ ID N° 836TTCTTTTGTTGGGTTTGTTTTGTTCAAGTTAGTAGTGAAGTTCAGCTCGAGTTCAACTCTTATCTGGACTCTATTGCTTTGGGATC MC210a TAACAATCAG ACTGCATCAAATTTCTACCT AGGCCTACAA No hit SEQ ID N° 837 TAATTTGAGT GTGGTCATGGGATGGGATC MC301 TAAACGATGC CCAACGACCA CCTTCTTTGG TCGTCCGTTC No hit SEQID N° 838 GTAGTCGTTT TCGACGTCAA GTTATCTTGG TGCGAGATTC GTTCTCGGACAGATTTGTTT ACAATCAAAG TTCGTCGTTG ATTCATCTCC GGTTAGTTGT TTTTGAGTTTTATTTTTGTC CAA MC305a TAACTGAACT TTATATAAAC TGTGCCGACA CCCTTCTCTC No hitSEQ ID N° 839 TTCACCTCCG GGGATGTGCT TACTGGTTGA GACTCCCTAT TCTGTTAGTGTCATACCTTG AAATAAGAAA GAGGCCGGAC AAGTTACGAA GCCAGATGGC CTTTTGGTTCCCGGTAAGTT GCCCCCTCCT CGACTCGAGT TGTCCGCTCG GGTACATAGT CTAAAACACTGACCCAGGTT TTGAACATAG AATAACGTGA CTTCATGCCG GATC MC306aTAACATGTTGGACGCGGATATACCTGTTCCAAATATACCAGAG No hit SEQ ID N° 840AGACCAATTTCTCTCATTGCGGATC MT104bAAGTCATAAAGAGGACTGAAAATTGCCAGAACCCTGAAGGAG No hit SEQ ID N° 841CTCCAGGATGACATCTGGCCAGAGCCTACTTGCTGCTGGGGCT GCACAAGCTGGGGGATC MT115aGTAATTGCTCATGTCCTATGCCTTTGGAAAGACATCCAAATGG No hit SEQ ID N° 842CTATGAGATTATATGCCCTCGTTAGACTTTGCCGGCAGATC MT116 AACATGTACC GGGATTCTCAAAGAAACAAG TCATAGCTAC No hit SEQ ID N° 843 ACCAGATGTT GATCATGTTCTTTTAGGAAT CTCGAAGAGA TTACTTCC MT117a TAATGTTATG ACTTGTGGGA GGGATTGTGTTTACAATGAT No hit SEQ ID N° 844 TGTAAAGATG ATTGTTGGAT TTGCTGTAGATGTGTTAGAT C MT117b TAATGTAGGT ATTGTGGGGT GGTAGTGGTT GGAGCTTCGA No hitSEQ ID N° 845 GAATTTGGGC AAAAAAGTGA CGGGAAAGTT TTCTAGATC MT118GAAGAACGGGATTCAAAAGGTAATTTCATTACTCAG No hit SEQ ID N° 846 MT209aTAAGGACGAG GAGGTAAAGG GGATTATTGG GTGTTAGTGT No hit SEQ ID N° 847GGGGTCAAGG AGACAGGCTA GGGCTTGGAG GGGAGATC MT210b TAAGGGAAAA GATAATTTTACTCCAGGACC AGAAGAAACT No hit SEQ ID N° 848 CAAAGACTGG TATGGAAAATTTTGAGATC MT213 GTCCCGAACTGTGCGTCTAGGCGGGTGGGGACACGGGGAGAA No hit SEQ IDN° 849 GGGGCACGATGGTTTTACCCAGGTTGGGGCCCTTTGGAGGGGGGTAAAACCCTCCTCCTGGTTGATTACTCAGGGCTCATT MT214b TTTAACCCAACCCTGTTATCAG Nohit SEQ ID N° 850 MT301a TAAACAGCCCGAAAATCACCCAAAGACACTCTCTAAACTATCC Nohit SEQ ID N° 851 AAAACATCGGCTTTGAATCACCCCAAAACCACGTTTTACGCAGCTAAAATACAGCACTAAACTCCCCAAAAAAGGGTCGATGTCGCACCATATTTGTCAACAAACAGAGCTTCGCTTCAACTGTATAAGATCACTCACGTTCAGTCGCGTTTTTTTTTTTAGTTGGGTTCAAGGTTTCCGACGTGGGTCTCGGGTCAGTAGTTTGTTTGTACGAA AGTTTTAGCAGATC MT303aAAGTGGCACTTTA No hit SEQ ID N° 852 MT303a CTTATTATGCTTTTGCTCGTTTA No hitSEQ ID N° 853 MT304a TAACGATTAT CCGTTTGGAA ACACTAGCAA AACCTGACGC No hitSEQ ID N° 854 CGGGACTCGC GAAAAATCGG AATAAGCCAA CAGGAATTCG TAGACCAAAACTCGAACATA CGGGGAACCT CAAATCCTCG AACGCGGACC AGATC MT304bTAACATACAGTACGAATTTTGTTACTTTATTACTTTGACAGCAA No hit SEQ ID N° 855TGCAAGGGAAAGCAGCCACAAGTTGGTAAGAAATAAAAAAAGGCCAAGAACACTAGTTGATGAGGATGTTGAGGACACCATGG CCAGATC MT304cTAACCAACTGAAACAAAACTGACACTATCATTGCATACAACCT No hit SEQ ID N° 856ACTGTCTACTATTGTTTTAAGTTTCTCTTCATTTTGTATTTTGATGTAATTGTATTATGGGACCACGTTTGTCACCGACCCTCTCCAG ATC MT305cTAACAACCAGAGTTCAAACGATAAAAGGGTGTGGTTGATTTAT No hit SEQ ID N° 857ACCCAACCCACTGAAACTTGAAAAATATACACTACTAGTCAAGCCATCAAGCAATCCAGAAATGCAGAGGAGCCCAGATC MT306bGCGTAGTGCTCTGTCGCTAATGGGGTTTATGCTGCGATTTTTCT No hit SEQ ID N° 858TTCCTGTCAAAAACTATGTGGACTAGGAGTGGAGTGCGTCCTCTACAACAATATCTGAGTTACATCCGATCGGGTCACTCAAGACT CA MT306cTAACGGAAGGAGAAAGGTGGATATTATTGTGGTGTGGCCTTTT No hit SEQ ID N° 859GTCTTTGGTTTTCTATTCTATTGAGCCCTAAAAATAGTGATATC TTGGTCTGATGTTCCCTGTTGCAGATCMT308c TAACCTTTTT TAGCAAGTAA TTAGATTACT AATTTCATTT No hit SEQ ID N° 860TTTAAAAACG GTTACCCGGA GTTTTCTTTT TTTTTTTTTA CACTTGCCAG ATC MT312TAAGGGTGAG CATGGAACTC GGGTGATATG GTAGCCTGTG No hit SEQ ID N° 861 AAMT313 TAATGCTGAT GA No hit SEQ ID N° 862 MT402aGGTGAATTGGCATTGGCTGCTCCAAAATGTCCTTCCATGAAAT No hit SEQ ID N° 863AAGAGCAATACCAGCATCTGCTGCTTATTTTCAAGACAAGATC MT408 TAATGTGTAA TCAGTAGCATCTATGTGCAA CTTTGATGGT No hit SEQ ID N° 864 TTTTGTCACA TCCAAGTAGTGAACACATTC ATCATTGGGA CCCTGATTTG TATTTGTTT MT410b TAATTGTATA TGAGTAACAAAAAAGAGTTA GTTGTATTTT No hit SEQ ID N° 865 ACTTTATCAC CGATTTCCCGAACTACGCAA GATC MAP1 CTGCCACCTCCAATCTCCAGGCATATACAGTCGCCAATTGCTT No hitSEQ ID N° 866 CTCCTCTTCCTATGTGTGCCTCTTCAAGTTCATGTACATGATCCATCTTCCTCTAATTTCTCTGGGCAAGATAATCATAACTATATCTTCACAACCTGAATCTAACTCCTCTGACCAAGATATACCAAAACCCATCTCCAATTTTGAACCATATCCATATCC MAP4a TTGGGGGAGG TTTGCGGCGG AGATAAAATGAAAAAAAAGA No hit SEQ ID N° 867 AATGGAAGTG AACTTGAAGT TA BMAP2aTAAGGTTCAAAGCCAGCTATTACAGTTAGTTGTGTGAGCTTAT No hit SEQ ID N° 868TCGCTCAACCTTAGCGGTAGGACATCTGGTGCGTCGGCTGCAT BMAP3CTCCGACTCGATGTTGTCCTTGGATTTGGATTCCGGAGAGATC No hit SEQ ID N° 869AAATGGTACAAACAGCTTGGAGGATATGACATCTGGACCGTCG GCTGCATA T304a GATCTGAGGTTGCTGATTTA GATTATGATG ACTTTGAGGC No hit SEQ ID N° 870 TGACTTTCAG GTCTTTAT226 GATCTCTA GTGTGAGTCA AAAATGATCA TAATATGAGT No hit SEQ ID N° 871TTTGCCGGAG GCTTGGTTCA ATGAAAAATC GTTGTTTCAG TTGAGGTTCA TTCTTTTTTACTGTTTCGCT CCTAATAAAT TTTTATTGTC AGTTGTCTTC TGATTTTTGC TGTTTTGTCCTATTCATTGT TGTGTTAGTA TTTTTGTTGA ATGTTGCATT GTTTTCTTTG TTTGAAAATTTCAATACGTT GGCCCTATCC TATTTTTGTA ATTTGTTTGG ATTATAATTG TATTGGTGTAGAAGATAAAA TTGTTCCATT A

TABLE 3 Primers used to amplify the NsPMT2 promoter Primer Code SequenceFwP ALGG52 5′-AAAAAGCAGGCTCGAGGAGTGGAATACGAACAAA-3′ RvP ALGG535′-AGAAAGCTGGGTTTTCCAAATTAAACTAAGCAAATTG-3′

TABLE 4 Jasmonate induction of the NsPMT2 promoter in transgenic BY-2cell line 7, represented as GUS activity in units/mg protein/minute.Time (h) +DMSO +MeJA 0 0.2 ± 0.3 0.8 ± 1.0 4 0.2 ± 0.3 2.0 ± 0.3 8 0.2 ±0.3 6.4 ± 0.3 14 0.2 ± 0.3 29.1 ± 1.9  24 2.9 ± 0.6 92.2 ± 6.4 

TABLE 5 Induction of the NsPMT2 promoter in transgenic BY-2 cell line 7,double transformed with pK7WGD2-C330, represented as GUS activity inunits/mg protein/minute. Line Time (h) +DMSO +MeJA BY-2 line 7 0 0.0 ±0.0  0.0 ± 0.0 24 0.9 ± 0.1  399.0 ± 56.4 48 6.0 ± 0.8  663.0 ± 33.6BY-2 line 7-C330 0 0.9 ± 0.1  1.0 ± 0.1 24 6.4 ± 0.1  276.7 ± 55.9 48128.6 ± 0.3  347.8 ± 2.0

TABLE 6 A: Measurement of nicotine alkaloids in BY-2 reporter cell linein the presence and absence of synthetic auxins, in the presence andabsence of MeJA. B: Measurement of nicotine alkaloids in BY-2 reportercell line supertransformed with an expression vector comprising C330, inthe absence of 2,4D, without and with the elicitor MeJA. Reporter cellline (line 7) + expression vector Anatabine Anabasine Nicotinecomprising the C330 gene mg/g DW Mg/g DW mg/g DW −2,4D + DMSO  0 h 0.036ND 0.010 24 h 0.018 ND 0.005 48 h 0.115 0.003 0.271 −2,4D + MeJA  0 h0.038 ND 0.008 24 h 2.065 0.099 0.271 48 h 3.541 0.297 0.283

TABLE 7 Seq code SEQUENCE SEQ ID N° MAP3 MNPANATESF SELDFLQSIENHLLNYDSDF SEIFSPMSSS SEQ ID N° 872 NALPNSPSSS FGSFPSAENS LDTSLWDENFEETIQNLEEK SESEEETKGH VVAREKNATQ DWRRYIGVKR RPWGTFSAEI RDPERRGARLWLGTYETPED AALAYDQAAF KIRGSRARLN FPHLIGSNIP KPARVTARRS RTRSPQPSSSSCTSSSENGT RKRKIDLINS IAKAKFIRHS WNLQMLL C330 MFPNCLPNEY NYTADMFFNDIFNEGIVGYG FEPASEFTLP SEQ ID N° 873 SIKLEPEMTV QSPAIWNLPE FVAPPETAAEVKLEPPAPQK AKHYRGVRVR PWGKFAAEIR DPAKNGARVW LGTYETAEDA AFAYDKAAFRMRGSRALLNF PLRINSGEPD PIRVGSKRSS MSPEYSSSSS SSASSPKRRK KVSQGTELTV LC484a MNNTTFSDPN SDTGGFLGSG KIGGFGYGIG VSVGILILIT SEQ ID N° 874TTTLTSYFCT RNQTSELPTR RQRTINRNEL SGHCVVDIGL DEKTLLSYPK LLYSEAKVNIKDSTASCCSI CLADYKKKDM LRLLPDCGHL FHLKCVDPWL MLNPSCPVCR TSPLPTPQSTPLAEVVPLAT RPLG C360 MGCIEKDPRE DVVQAWYMDD SDEDQRLPHH REPKEFVSLD SEQ IDN° 875 KLAELGVLSW RLDADNYETD EELKKIREAR GYSYMDFCEV CPEKLPNYEE KIKNFFEEHLHTDEEIRYCV AGSGYFDLRD RNDAWIRVWV KKGGMIVLPA GIYHRFTLDS DNYIKAMRLFVGDPIWTPYN RPHDHLPARK EYIESFIQAE GAGRAVNAAA C165 MFFAHRENTM STLGRLVLIFWLFVVLIINS SYTASLTSIL SEQ ID N° 876 TVQQLSSGIQ GIDSLISSSD QIGVQDGSFAYNYLIEELGV SESRLRILKT EDEYVSALEK GPHGGGVAGI VDELPYVELF LSNNKCIFRTVGQEFIKGGW GFAFQRDSPL AVDLSTAILQ RSENGELQRI HDKWLTNNGC SSQNNQADDTQLSLKSFWGL FLICAIACVL ALIVFFCRVY CQFRRYHPEP EEPEISEPES ARPSRRTLRSVSFKDLIDFV DRRESEIKEI LKRKSSDNKR HQTQNSDGQP SSPV C353a MNPEYDYLFKLLLIGDSGVG KSCLLLRFAD DSYLESYIST SEQ ID N° 877 IGVDFKIRTV EQDGKTIKLQIWDTAGQERF RTTTSSYYRG AHGIIVVYDV TDQESFNNVK QWLSEIDRYA SDNVNKLLVGNKCDLTAQKV VSTEIAQAFA DEIGIPFMET SAKNATNVEQ AFMAMAASIK NRMASQPASSNARPPTVQIR GQPVNQKSGC CSS MT101 MRVRIHQTMA TVMQKIKDIE DEMAKTQKNKATAHHLGLLK SEQ ID N° 878 AKLAKLRREL LTPTSKGGGG AGEGFDVTKS GDARVGLVGFPSVGKSTLLN KLTGTFSEVA SYEFTTLTCI PGVIMYRGAK IQLLDLPGII EGAKDGKGRGRQVISTARTC NCILIVLDAI KPITHKRPIE KELEGFGIRL NKEPPNLTFR RKEKGGINLTSTVTNTHLDL DTVKAICSEY RIHNADVHLR YDATADDLID VIEGSRVYTP CIYVVNKIDQIPMEELEILD KLPHYCPISA HLEWNLDGLL EKIWEYLSLT RIYTKPKGMN PDYEDPVILSSKRRTVEDFC DRIHKDMVKQ FKYALVWGSS AKHKPQRVGR EHELEDEDVV QIIKKV T21MANPKVFFDL TVGGLPTGRV VMELFNDVVP KTADNFRALC SEQ ID N° 879 TGEKGVGKSGKPLHYKGSSF HRVIPGFMCQ GGDFTAGNGT GGESIYGAKF ADENFVKKHT GPGILSMANAGPGTNGSQFF ICTAKTEWLD GKHVVFGQVI EGMDVIKKVE AVGSSSGRCS KPVVIADCGQ LSC476a MALVRERRQL NLRLPLPEPS ERRPRFPLPL PPSISTTTTA SEQ ID N° 880PTTTISISEL EKLKVLGHGN GGTVYKVRHK RTSAIYALKV VHGDSDPEIR RQILREISILRRTDSPYVIK CHGVIDMPGG DIGILMEYMN VGTLESLLKS QATFSELSLA KIAKQVLSGLDYLHNHKIIH RDLKPSNLLV NREMEVKIAD FGVSKIMCRT LDPCNSYVGT GAYMSPARFDPDTYGVNYNG YAADIWSLGL TLMELYMGHF PFLPPGQRPD WATLMCAICF GEPPSLPEGTSGNFRDFIEC CLQKESSKRW SAQQLLQHPF ILSIDLKST MC204 MYGRSGLDRF KKAQSLEPFQVSANSAAKPA LQPTTKAVTH SEQ ID N° 881 PFPAYAQSTF SHQQTQYVNP QPALQKSVAADATASTVPTH HVTHGGGQST WQPPDWAIEP RPGVYYLEVI KDGEVLDRIN LDKRRHIFGRQFHTCDFVLD HQSVSRQHAA VIPHKNGSIY VIDLGSAHGT FVANERLTKD SPVELEPGQSLKLAVSTRPY ILRRNNDALF PPPRQLAEID FPPPPDPSDE EAVLAYNTFL NRYGLIRPDSLSKSTVSTSG EDVNYSSDRR AKRIRRTSVS FKDQVGGELV EVVGISDGAD VETEPGPLGVKEGSLVGKYE SLIEPTVLPK GKEQSSVKDA TVTRTGVSDI LQQVLSKVKN PPKGGIYDDLYGESAPAKGG FWAYSDSSQT ASTNDAKGDS PCSLRRIFGH ISNNVDDDTD DLFG T323MHSANHWGGS LEIANTGDST AEEYDRSRNL DWDRASVNHH SEQ ID N° 882 QKQQQYNNYDQYSHRHNLDE TQQSWLLGPP EKKKKKYVDL GCIVCSRKAF KYTIYGIIIA FLVIALPTIIAKSLPKHKTR PSPPDNYTIA LHKALLFFNA QKSGKLPKNN EIPWRGDSGL QDGSKLTDVKGGLIGGYYDA GDNTKFHFPM SFAMTMLSWS VIEYEHKYRA IDEYDHIRDL IKWGTDYLLRTFNSTATK1D KLYSQVGGSL NNSRTPDDHY CWQRPEDMNY ERPVQTANSG PDLAGEMAAALAAASIXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXXXXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXXXRRNCGPRYI SLDILRRFAT SQMNYILGDN PLKMSYVVGY GNKFPRHVHH RGASIPSGKTKYSCTGGWKW RDTKNPNPHN ITGAMVGGPD KFDKFKDARK NFSYTEPTLA GNAGLVAALVSLTSSGGYGV DKNAIFSAVP PLYPMSPPPP PPWKP T464 MGSSGGMDYG AYTYENLEREPYWPTEKLRI SITGAGGFIA SEQ ID N° 883 SHIARRLKSE GHYIIASDWK KNEHMTEDMFCHEFHLVDLR VMDNCLKVTK DVDHVFNLAA DMGGMGFIQS NHSVIFYNNT MISFNMMEAARINGVKRFFY ASSACLYPEF KQLETNVSLK ESDAWPAEPQ DAYGLEKLAT EELCKHYNKDFGIECCIGRF HNIYGPFGTW KGGREKAPAA FCRKAQTAVD KFEMWGDGLQ PRSFTFIDECVEGVLRLTES DFREPVNIGS DEMVSMNDMA EMVISFEDKK LPVHHIPGPE GVSGRNSDNTLIKEKLGWAP TMRLKDGLRI TYFWIKEQIE KERSQGVNIA NYGSYKVVGT QAPVELGSLR AADGKEC127 MERNVANEAP KATIMAEDYK KDLEFIEEVT SNVDEVQMRV SEQ ID N° 884LAEILSQNAH VEYLQRHNLN GSTDRETFKK VVPVITYEDI QPDIKRIAYG DKSPILCSQPISELLSSSGT SGGESKLIPT TEPEIGKRLQ LHKLVMSVLS QVAPDSGKGK GMYFMFISPEQKTPGGLIAR FLTTSYYNSP YFNYSRLHNP HCNYTSPTAA ILCPDSYQSM YSQMLCGLCQNNQVLRVGSF FATSFVRAIR FLEKHWSLLC NDIRSGTINT QITDPLVREA VMEVLKPDPTLADFIEVECT KDSWQGIITR LWRNTKYVDV IVTGSMSQYI PILDYYSNNL PLISTLYASSESHFGINLNP FCKPSDVSYT LIPTMCYFEF LPYRGNSGVI DSISMPKSLN EKEQQQLVDLADVKIGQEYE LVVTTYSGLY RYRVGDVLQV AGYKNNAPRF NFLCRENYVL SIGADFTNEVELQNAVKNAV GNLVPFDSQV TEYTSYVDIT TLPSHYVIFW ELNANDSTLV PPSVFEDCCLTIEESLNYFY REGRASNESI GPLEIRVLEI GTFDKLMDYC MSLGASMNQY KTPRCLKYAPLIELLNSRVV SSYFSPMCPK WVPGYKKWDG NN C175 MERSVANEAP KATIMVEDYKKNIEFIEEVT SNVDEVQMRV SEQ ID N° 885 LAEILSQNAH VEYLQRYNLN GRTDRETFKKVVPVITYEDI QPDIKRIAYG DKSPILCSQP ISELLSSSGT SGGESKLIPS TEAALGRRLQLLKLLMSVMS QVAPDFGKGK GMYFMFISSE QKTPGGLLAR FFTTSFYKSP YINCGYPCRKFTSPTATILC QDSYQSMYSQ MLCGLCQNQE VLRVGSLFAT GFIRGIRFLE KHWSLLCNDIRNGTINTQIT DPSVREAVME ILKPDPKLAD FIEAECSKDS WQGIITRLWP NTKYVDAILTGSMSQYLPIL DYYSNSLPLI STLYGSSECH FGINLNPFCK PSEVSYTLIP TMCYFEFLPYHGNSGVIDSI SMPKSLNEKE QQQLVDLADV EIGQEYELVV TTYSGLYRYR VGDVLRVAGYKNNAPRFNFL CRENVILSIG ADFTNEVELQ NAVKNAVGNL MPFDSQVTEY TGYVDITTIPSHYVIFWELN ANDSTPVPPS VFEDCCLTIE ESLNYFYREG RASNASIGPL EIRVVEIGTFDKLMDYCSSL GASMNQYKTP RCVKYAPLIE LLNSRVVSRY FSPMCPKWVP GYKKWNNTS T424bMAKEGTKVPR IKLGSQGLEV SAQGLGCMGM SAFYGPPKIPE SEQ ID N° 886 PDMIQLIHHSINSGVTFLDT SDVYGPHTNE ILLGKALKGG VRERVELATK FGAIFADGKI KVCGEPAYVRAACEASLKRL DVDCIDLYYQ HRIDTRVPIE VTVGELKKLV EEGKIKYIGL SEASASTIRRAHAVHPITTV QLEWSLWSRD VEEEIIPTCR ELGIGIVAYS PLGRGFLSSG PELLEDLSSEDFPKHLPRFQ ADNLEHNKIL YERICQMAAK KGCTPSQLAL AWVHHQGNDV CPIPGITKIENLNQNIGALS IKLTTEDMVE LEYIASADAV KGERDASGAN HKNSDTPPLS TWKATR T164MESNNVVLLD FWPSSFGMRL RIALALKGIK YEAKEENLSD SEQ ID N° 887 KSPLLLEMNPVHKKIPILIH NSKAICESLN ILEYIDEVWH DKCPLLPSDP YERSQARFWA DYIDKKIYSTGRRVWSGKGE DQEEAKKEFI EILKTLEGEL GNKTYFGGDN LGFVDVALVP FTSWFYSYETCANFSIEAEC PKLVVWAKTC MESESVSKSL PHPHKIYGFV LELKHKLGLA MAP2 MSDGGLTVLDGSQLRAVSLS LPSSDGSSVT GAQLLDFAES SEQ ID N° 888 KVSESLFGFS LPDTLKSAALKRLSVADDLN FRREQLDREN ASIILRNYVA AIADELQDDP IVIAILDGKT LCMFLEDEDDFAMLAENLFT DLDTEDRGKI RRNQIRDALI HMGVEMGIPP LSEFPILSDI LKRHGAEGEDELGQAQFAHL LQPVLQELAD ALAKNPVVVV QKIKINNGSK LRKVLADEKQ LSETVEKIMQEKQDEKDSLS NKDAIRCYLE KNGASLGLPP LKNDEVVILL YDIVLGDIEN GKTDAASDKDEILVFLKDIL EKFAAQLEVN PTFHDFDN C1 MATKVYIVYY SMYGHVEKLA EEIKKGAASVEGVEAKLWQV SEQ ID N° 889 PETLSEDVLA KMSAPPKSDV AVITPQELAE ADGIIFGFPTRFGMMAAQFK AFLDATGGLW RTQQLAGKPA GIFYSTGSQG GGQETTPLTA ITQLVHHGMIFVPIGYTFGA GMFEMEKVKG GSPYGAGTFA GDGSRQPSDL ELQQAFHQGK YIAGIAKKLK GAAT210 MKIVDLDESL MESDGNCVNT EKRLIVVGVD AKRALVGAGA SEQ ID N° 890RILFYPTLLY NVFRNKIQSE FRWWDQIDQF LLLGAVPFPS DVPRLKQLGV GGVITLNEPYETLVPSSLYH AHGIDHLVIP TRDYLFAPSF VDINRAVDFI HRNASIGQTT YVHCKAGRGRSTTVVLCYLV EYKHMTPRAA LEFVRSRRPR VLLAPSQWKA VQEFKQQRVA SYALSGDAVLITKADLEGYH SSSDDSRGKE LAIVPRIART QPMIARLSCL FASLKVSDGC GPVTRQLTEA RACC112 MSSASTENRS LWTEIRESIR SILKANCGHF HTLFILFLLP SEQ ID N° 891IFFSLVVYPS FHLALFHPDY DFTQPVQFSH FLSSHFEIIV PIVFTLFLVL LFLCAVATTTYSALHVSYGR PINLVSSIKS IRNSFFPLLS TFIVSHTIFI SIALVFSLVL VFLVQVLQTLGLIELKYDSN HFLFLVIPAL IVLVPVLIWL QVNWSLAYVI AVVESKWGFE TLRRSAYLVKGKRSVALSMM LLYGLLMGIM VVLGAMYLVI MDAAKGRQWR SSGVILQTAM SSITSYLMMSQFLVGNVVLY LRCNDLNGEK LPLEIEHLLL HQSLANDHPP PMLSASTKNL SLWTEVVESAMSIFKANSGH FHALSILFLL PISFFLVVYP SFHLALFHPN YDFISFAQPH LFLSNFEIIVPTSYSLFLVL LFLCAVATTT YSAVHASYSR PINLVLSIKS IRKSLFPLLS TLLVSHTIFISITLVFTLVL TILVQILQPL GLIEIKYDSD HFLLLAIPAL VVLVPVLLWL HVNWSLAYVIAVIESKWGYE TLRRSSYLVK GQRWVAFGIY LYYGLSMGIM MVCGSMFFVI MGVAKGNKWRSLDVIIQTAL VSVMGYLTMN QYLVANVVLY MKCKDLSVEK LQSETGGEYV PLPLDEKNQA LEC454 SQFFSSIPLQ PIPRGSSFAA STIHSGPIPA RISSTYPCSG SEQ ID N° 892PIERGFMSGP IERSFTSGPL ENQYDHIQRY KPKSKKWGLI KSLKKVLSNS FLGFNKFMNLVEKNNNNEVN VQGSNSHHSN VGNSLSSQNS LVDDDDEGND SFRGQNVQWA QGKAGEDRVHVVISEEHGWV FVGIYDGFNG PDATDFLLNN LYSNVYKELK GLLWNDKLKT PKNSTSNETVPLRNSGFKVE HFVQNQELDQ REKLDGVVGV DHSDVLKALS EGLRKTEASY LEIADMMVKENPELALMGSC VLVMLLKDQD VYLLNVGDSR AVLAQNPESD ISISKLKRIN EQSVNSIDALYRAESDRKHN LIPSQLTMDH STSIKEEVIR IRSEHLDDPF AIKNDRVKGS LKVTRAFGAGYLKQPKWNNA LLEMFRINYI GNSPYINCLP SLYHHTLGSR DRFLILSSDG LYQYFTNEEAVSEVETFMSI FPEGDPAQHL VEEVLFRAAK KAGLNFHELL DIPQGDRRKY HDDVSIIILSFEGRIWKSSL T172 GAENGLIVSD SIIQGNEEDE ILSVGEDPCV INGEELLPLG SEQ ID N°893 ASSELSLPIA VEIEGIDNGQ ILAKVISLEE RSFERKISNL SAVAAIPDDE ITTGPTLKASVVALPLPSEN EPVKESVKSV FELECVPLWG SVSICGKRPE MEDALMVVPN FMIUPIKMFIGDRVIDGLSQ RLSHLTSHFY GVYDGHGGSQ VADYCCKRIH LALVEELKLF KDDMVDGSAKDTRQVQWEKV FTSCFLKVDD EVGGKVNSDP GEDNIDTTSC ASEPIAPETV GSTAVVAVICSSHIVVSNCG DSRAVLYRGK EAMALSIDHK PSREDEYARI EASGGKVIQW NGHRVFGVLAMSRSIGDRYL KPWIIPEPEI MFVPRAREDE CLVLASDGLW DVMSNEEACE VARRRILLWHKKNGTNPLPE RGQGVDPAAQ AAAEYLSTMA LQKGSKDNIS VIVVDLKAQR KFKSKC C477METQNLERGH VIEVRCDMAA QEKGTKICGS APCGFSDVNT SEQ ID N° 894 MSKDAQERSASMRKLCIAVV LCIIFMAVEV VGGIKANSLA ILTDAAHLLS DVAAFAISLF SLWAAGWEDNPRQSYGFFRI EILGALVSIQ MIWILAGILV YEAIARLIHD TGEVQGFLMF VVSAFGLVVNLIMALLLGHD HGHGHGHGHS HGHDHEHGHN HGEHAHSNTD HEHGHGEHTH IHGISVSRHHHHNEGPSSRD QHSHAHDGDH TVPLLKNSCE GESVSEGEKK KKPQNINVQG AYLHVIGDSIHSIGVMIGGA IIWYKPEWKI IDLICTLLFS VIVLGTTIRM LRSILEVLME STPREIDATRLQKGLCEMED VVPIHELHIW AITVGKVLLA CHVKIKSDAD ADTVLDKV C331 MLIMLLVPVRQYLLPKFFKG AHLQDLDAAE YEEAPAIAYN SEQ ID N° 895 MSYGDQDPQA RPACIDSSEILDEIITRSRG EIRHPCSPRV TSSTPTKLEE IKSMHSPQLA QRAYSPRVNV LRGERSPRLTGKGLGIKQTP SPQPSNLGQN GRGPSST

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1. An isolated polypeptide that modulates the production of at least one secondary metabolite in an organism or cell derived therefrom wherein said polypeptide is selected from the group consisting of: (a) a polypeptide encoded by a polynucleotide comprising SEQ ID NO: 1 through 611 or SEQ ID NO: 612 through 871; (b) a polypeptide comprising a polypeptide sequence having a least 60% identity to at least one of the polypeptides encoded by a polynucleotide sequence having SEQ ID NO: 612 through 871; (c) a polypeptide comprising a polypeptide sequence having a least 90% identity to at least one of the polypeptides encoded by a polynucleotide sequence of SEQ ID NO: 1 through SEQ ID NO:610, or SEQ ID NO:611; (d) fragments and variants of the polypeptides according to (a), (b) or (c) that modulate the production of at least one secondary metabolite in an organism or cell derived thereof.
 2. The isolated polypeptide of claim 1 wherein said isolated polypeptide is selected from the group consisting of SEQ ID NOs: 872, 873, 874 through 895 and polypeptide sequences having at least 90% identity to SEQ ID NO: 872, 873, 874 through
 895. 3. An isolated polynucleotide selected from the group consisting of: (a) a polynucleotide comprising a polynucleotide sequence having at least one of the sequences SEQ ID NO: 1 through SEQ ID NO: 611 or SEQ ID NO: 612 through 871; (b) a polynucleotide comprising a polynucleotide sequence having at least 60% identity to at least one of the sequences having SEQ ID NO: 612 through SEQ ID NO: 871; (c) a polynucleotide comprising a polynucleotide sequence having at least 90% identity to at least one of the sequences having SEQ ID NO: 1 through SEQ ID NO: 611; (d) fragments and variants of the polynucleotides of the foregoing (a), (b) or (c), modulating the production of at least one secondary metabolite in an organism or cell derived thereof.
 4. A recombinant DNA vector comprising at least one of the polynucleotide sequences of claim
 3. 5. A transgenic plant or a cell derived therefrom transformed with the recombinant DNA vector of claim
 4. 6. A method of identifying genes, the expression of which modulates the production of at least one secondary metabolite in an organism or cells derived from said organism, said method comprising the steps of: (a) performing a genome wide expression profiling of said organism or cells on different times of growth, (b) isolating genes whose expression is co-regulated either with said at least one secondary metabolite, or with a gene known to be involved in the biosynthesis of said secondary metabolite, (c) analysing the effect of over- or under-expression of said isolated genes in said organism or cell on the production of said at least one secondary metabolite, and (d) identifying genes that can modulate the production of said at least one secondary metabolite.
 7. The method according to claim 6, wherein steps (a) to (d) are preceded by a step of inducing the production of said at least one secondary metabolite in said organism or cell.
 8. The method according to claim 6 wherein said secondary metabolite is an alkaloid or phenylpropanoid.
 9. The method according to claim 7 wherein said secondary metabolite is an alkaloid or phenylpropanoid.
 10. A method of modulating a cell, comprising using the polynucleotide of claim 3 to modulate the biosynthesis of secondary metabolites in the cell.
 11. A method of modulating the biosynthesis of alkaloids in a cell, said method comprising: using a polynucleotide comprising SEQ ID NO: 10, 11, 19, 20, 35, 40, 41, 47, 65, 67, 70, 88, 89, 97, 98, 101, 102, 103, 106, 107, 108, 117, 118, 120, 121, 123, 124, 126, 128, 130, 131, 132, 136, 137, 142, 143, 144, 145, 146, 147, 148, 152, 154, 155, 159, 160, 161, 162, 163, 175, 176, 177, 181, 182, 183, 189, 197, 202, 207, 208, 209, 210, 217, 219, 220, 221, 233, 235, 236, 237, 239, 240, 241, 242, 243, 244, 261, 262, 264, 265, 268, 70, 272, 273, 274, 278, 279, 299, 300, 302, 303, 304, 305, 306, 316, 317, 318, 320, 321, 326, 329, 331, 332, 333, 334, 341, 344, 348, 349, 350, 351, 354, 355, 356, 358, 372, 373, 374, 375, 377, 382, 390, 391, 392, 395, 403, 405, 406, 414, 417, 418, 419, 420, 424, 430, 434, 439, 440, 441, 445, 446, 456, 463, 478, 485, 491, 497, 507, 508, 510, 518, 519, 527, 529, 531, 532, 534, 567, 569, 570, 575, 577, 579, 587, 593, 594, 598, 599, 601, 603, 608, 612, 613, 618, 619, 620, 628, 636, 642, 643, 647, 648, 649, 652, 653, 654, 655, 656, 657, 659, 660, 662, 664, 670, 671, 674, 675, 676, 677, 679, 680, 682, 683, 695, 696, 700, 701, 703, 707, 709, 710, 711, 712, 714, 719, 724, 727, 729, 732, 734, 735, 740, 741, 744, 746, 748, 749, 750, 751, 753, 754, 755, 757, 758, 759, 760, 761, 762, 763, 764, 766, 767, 772, 777, 784, 794, 809, 810, 811, 816, 817, 822, 823, 826, 827, 828, 829, 830, 832, 833, 834, 836, 837, 839, 840, 841, 850, 854, 855, 856, 858, 859, 861, 864, 865, 488, 489 and/or 490 or fragments or homologues thereof to modulate the biosynthesis of alkaloids in the cell.
 12. A method of modulating the biosynthesis of phenylpropanoids in a cell, said method comprising: using a polynucleotide comprising SEQ ID NO: 3, 4, 5, 7, 15, 17, 21, 23, 29, 30, 32, 33, 39, 42, 44, 45, 46, 48, 49, 50, 51, 8, 61, 62, 72, 74, 79, 84, 92, 94, 95, 104, 105, 125, 134, 150, 170, 171, 179, 180, 184, 194, 195, 200, 201, 203, 204, 205, 213, 214, 215, 218, 245, 249, 250, 251, 252, 254, 255, 266, 275, 276, 281, 282, 285, 286, 287, 289, 291, 298, 301, 308, 309, 310, 311, 312, 313, 315, 319, 323, 324, 335, 343, 361, 363, 364, 370, 379, 380, 383, 384, 385, 386, 398, 401, 402, 407, 415, 416, 423, 432, 433, 437, 443, 444, 447, 448, 450, 451, 452, 455, 457, 460, 461, 462, 471, 474, 486, 487, 493, 494, 499, 500, 501, 502, 503, 504, 505, 506, 517, 522, 523, 524, 526, 528, 538, 541, 543, 544, 545, 546, 547, 553, 554, 555, 562, 568, 571, 572, 578, 580, 581, 582, 588, 605, 607, 616, 617, 621, 626, 627, 637, 638, 641, 644, 650, 651, 665, 666, 667, 681, 684, 685, 691, 697, 698, 704, 708, 713, 720, 721, 728, 730, 736, 745, 752, 756, 771, 776, 778, 782, 783, 792, 793, 795, 797, 798, 799, 800, 801, 808, 815, 818, 819, 820, 821, 835, 842, 843, 844, 845, 848, 851, 852, 853, 862, 868, 488, 489 and/or 490 or fragments or homologues thereof to modulate the biosynthesis of phenylpropanoids in the cell. 